新疆农业科学 ›› 2023, Vol. 60 ›› Issue (5): 1208-1215.DOI: 10.6048/j.issn.1001-4330.2023.05.020
收稿日期:
2022-09-25
出版日期:
2023-05-20
发布日期:
2023-05-22
通信作者:
冯宏祖(1968-),男,青海海东人,教授,博士,研究方向为农业有害生物综合防治,(E-mail)fhzzky@163.com作者简介:
姬瑞(1995-),女,甘肃天水人,硕士研究生,研究方向为果树生理与高效栽培,(E-mail)2142949280@qq.com
基金资助:
JI Rui(), WANG Lan, WANG Zhe, GOU Changqing, HAO Haiting, FENG Hongzu(
)
Received:
2022-09-25
Published:
2023-05-20
Online:
2023-05-22
Supported by:
摘要:
【目的】在库尔勒香梨授粉液中添加几丁聚糖,研究植物免疫诱抗剂几丁聚糖对花粉活力、香梨树坐果率及果实品质的影响,筛选出最佳的几丁聚糖喷施浓度,为生产上合理利用几丁聚糖提高香梨坐果率及改善果实品质提供理论依据。【方法】以授粉花粉(鸭梨花粉)与库尔勒香梨树为试材,采用花粉离体萌法和田间人工液体授粉法,研究7个浓度的几丁聚糖处理下授粉花粉活力、香梨坐果率及果实品质的变化。【结果】几丁聚糖浓度在0.25~1.25 mL/L时能够提高花粉萌发率、坐果率及改善果实品质,几丁聚糖浓度达到2.50~5.00 mL/L时会导致花粉萌发率、坐果率及果实品质的下降。0.5 mL/L几丁聚糖在提高花粉萌发率和坐果率的同时改善香梨品质。【结论】香梨授粉液中以添加0.5 mL/L几丁聚糖为最适用量。
中图分类号:
姬瑞, 王兰, 王喆, 苟长青, 郝海婷, 冯宏祖. 授粉液中添加几丁聚糖对库尔勒香梨树坐果率及果实品质的影响[J]. 新疆农业科学, 2023, 60(5): 1208-1215.
JI Rui, WANG Lan, WANG Zhe, GOU Changqing, HAO Haiting, FENG Hongzu. Effects of chitosan to pollination solution on fruit setting rate and fruit quality of Korla Fragrant Pear[J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1208-1215.
图1 不同浓度几丁聚糖处理2 h下库尔勒香梨授粉花粉(鸭梨花粉)萌发率变化 注:同列不同字母表示差异显著 (P<0.05),下同
Fig.1 Effects of different concentrations of chitosan treatment for 2h on the germination rate of pollinated pollen of Kuerlexiangli (Yali pear pollen ) Note : Different letters in the same column indicate significant difference (P< 0.05 ),the same as below
图2 不同浓度几丁聚糖处理2 h下授粉花粉萌发状况 注:A.0 mL/L;B.0.25 mL/L;C.0.50 mL/L;D.1.00 mL/L;E.1.25 mL/L;F.2.50 mL/L;G.5.00 mL/L
Fig.2 Pollen germination after 2 h treatment with different concentrations of chitosan
处理 Treatment | 单果重 Fruit weight(g) | 纵径 Vertical diameter(mm) | 横径 Horizontal diameter(mm) | 果形指数 Shape index | 硬度 Hardness (N) |
---|---|---|---|---|---|
CK | 128.03±7.01b | 67.51±0.92b | 57.50±0.89b | 1.17±0.03c | 11.87±0.69a |
J1 | 133.06±12.79a | 64.58±2.85c | 53.80±2.75c | 1.20±0.02a | 10.10±0.57c |
J2 | 136.25±10.72a | 71.20±2.53a | 59.14±2.76a | 1.21±0.04a | 10.53±0.66bc |
J3 | 136.00±11.95a | 71.83±2.44a | 59.85±2.81a | 1.20±0.03a | 9.90±0.45c |
J4 | 132.73±10.30ab | 63.47±2.55c | 53.35±2.49c | 1.19±0.06b | 11.51±0.16ab |
J5 | 132.08±10.90ab | 59.21±1.85d | 50.33±2.84d | 1.18±0.07bc | 11.70±0.26a |
J6 | 131.66±9.47ab | 63.82±1.74c | 54.11±1.51c | 1.18±0.04bc | 11.98±0.27a |
表1 不同浓度几丁聚糖处理下库尔勒香梨外在品质变化
Tab.1 Changes in external quality of Kuer Fragrant Pear treated with different concentrations of chitosan
处理 Treatment | 单果重 Fruit weight(g) | 纵径 Vertical diameter(mm) | 横径 Horizontal diameter(mm) | 果形指数 Shape index | 硬度 Hardness (N) |
---|---|---|---|---|---|
CK | 128.03±7.01b | 67.51±0.92b | 57.50±0.89b | 1.17±0.03c | 11.87±0.69a |
J1 | 133.06±12.79a | 64.58±2.85c | 53.80±2.75c | 1.20±0.02a | 10.10±0.57c |
J2 | 136.25±10.72a | 71.20±2.53a | 59.14±2.76a | 1.21±0.04a | 10.53±0.66bc |
J3 | 136.00±11.95a | 71.83±2.44a | 59.85±2.81a | 1.20±0.03a | 9.90±0.45c |
J4 | 132.73±10.30ab | 63.47±2.55c | 53.35±2.49c | 1.19±0.06b | 11.51±0.16ab |
J5 | 132.08±10.90ab | 59.21±1.85d | 50.33±2.84d | 1.18±0.07bc | 11.70±0.26a |
J6 | 131.66±9.47ab | 63.82±1.74c | 54.11±1.51c | 1.18±0.04bc | 11.98±0.27a |
处理 Treatment | 可溶性固形物 Soluble solid (%) | 可溶性糖 Soluble sugar (%) | 可滴定酸 Titratable acidity (%) | VC (mg/100g) | 固酸比 Solid acid ratio | 糖酸比 Sugar acid ratio |
---|---|---|---|---|---|---|
CK | 11.66±0.89bc | 11.35±0.12bc | 0.58±0.05bc | 5.53±0.16c | 20.15±1.56bc | 19.70±1.55bc |
J1 | 12.90±0.46a | 11.38±0.07bc | 0.39±0.02d | 6.12±0.06ab | 33.32±2.27a | 29.38±1.85a |
J2 | 13.10±0.97a | 11.70±0.04a | 0.36±0.02d | 6.20±0.10ab | 34.64±2.00a | 32.46±2.06a |
J3 | 13.21±0.09a | 11.41±0.06b | 0.38±0.04d | 6.03±0.06abc | 36.66±2.43a | 30.35±2.98a |
J4 | 12.60±0.46ab | 11.72±0.06a | 0.57±0.03c | 5.91±0.06abc | 22.24±0.91b | 20.71±0.90b |
J5 | 11.34±0.14c | 11.23±0.04cd | 0.64±0.03ab | 6.41±0.06a | 17.68±0.83c | 17.49±0.81bc |
J6 | 11.27±0.09c | 11.10±0.06d | 0.67±0.02a | 5.74±0.06bc | 16.72±0.53c | 16.47±0.49c |
表2 不同浓度几丁聚糖处理下库尔勒香梨内在品质变化
Tab.2 Changes of internal quality of Kuerle Fragrant Pear different concentrations of chitosan treatment
处理 Treatment | 可溶性固形物 Soluble solid (%) | 可溶性糖 Soluble sugar (%) | 可滴定酸 Titratable acidity (%) | VC (mg/100g) | 固酸比 Solid acid ratio | 糖酸比 Sugar acid ratio |
---|---|---|---|---|---|---|
CK | 11.66±0.89bc | 11.35±0.12bc | 0.58±0.05bc | 5.53±0.16c | 20.15±1.56bc | 19.70±1.55bc |
J1 | 12.90±0.46a | 11.38±0.07bc | 0.39±0.02d | 6.12±0.06ab | 33.32±2.27a | 29.38±1.85a |
J2 | 13.10±0.97a | 11.70±0.04a | 0.36±0.02d | 6.20±0.10ab | 34.64±2.00a | 32.46±2.06a |
J3 | 13.21±0.09a | 11.41±0.06b | 0.38±0.04d | 6.03±0.06abc | 36.66±2.43a | 30.35±2.98a |
J4 | 12.60±0.46ab | 11.72±0.06a | 0.57±0.03c | 5.91±0.06abc | 22.24±0.91b | 20.71±0.90b |
J5 | 11.34±0.14c | 11.23±0.04cd | 0.64±0.03ab | 6.41±0.06a | 17.68±0.83c | 17.49±0.81bc |
J6 | 11.27±0.09c | 11.10±0.06d | 0.67±0.02a | 5.74±0.06bc | 16.72±0.53c | 16.47±0.49c |
指标 Index | 主成分1 Principal component 1 | 主成分2 Principal component 2 | 主成分3 Principal component 3 |
---|---|---|---|
单果重 Fruit weight | 0.291 | -0.274 | -0.095 |
纵径 Vertical diameter | 0.261 | 0.537 | -0.043 |
横径 Horizontal diameter | 0.228 | 0.615 | -0.045 |
果形指数 Shape index | 0.327 | -0.239 | 0.022 |
硬度 Hardness | -0.319 | 0.076 | 0.319 |
可溶性固形物 Soluble sugar | 0.332 | -0.004 | 0.190 |
可溶性糖 Soluble sugar | 0.215 | -0.022 | 0.905 |
可滴定酸 Titratable acidity | -0.338 | -0.048 | 0.092 |
维生素C Vitamin C | 0.284 | -0.437 | -0.080 |
固酸比 Solid acid ratio | 0.343 | 0.015 | -0.083 |
糖酸比 Sugar acid ratio | 0.342 | 0.031 | -0.093 |
特征值 Eigenvalue | 8.279 | 1.423 | 0.749 |
贡献率 Contribution rates(%) | 75.265 | 12.939 | 6.812 |
累积贡献率 Cumulative variance(%) | 75.265 | 88.204 | 95.017 |
权重 Weight | 0.792 | 0.136 | 0.072 |
表3 各果实品质指标主成分分析的特征向量
Tab.3 Characteristic vector of principal component analysis of fruit quality index
指标 Index | 主成分1 Principal component 1 | 主成分2 Principal component 2 | 主成分3 Principal component 3 |
---|---|---|---|
单果重 Fruit weight | 0.291 | -0.274 | -0.095 |
纵径 Vertical diameter | 0.261 | 0.537 | -0.043 |
横径 Horizontal diameter | 0.228 | 0.615 | -0.045 |
果形指数 Shape index | 0.327 | -0.239 | 0.022 |
硬度 Hardness | -0.319 | 0.076 | 0.319 |
可溶性固形物 Soluble sugar | 0.332 | -0.004 | 0.190 |
可溶性糖 Soluble sugar | 0.215 | -0.022 | 0.905 |
可滴定酸 Titratable acidity | -0.338 | -0.048 | 0.092 |
维生素C Vitamin C | 0.284 | -0.437 | -0.080 |
固酸比 Solid acid ratio | 0.343 | 0.015 | -0.083 |
糖酸比 Sugar acid ratio | 0.342 | 0.031 | -0.093 |
特征值 Eigenvalue | 8.279 | 1.423 | 0.749 |
贡献率 Contribution rates(%) | 75.265 | 12.939 | 6.812 |
累积贡献率 Cumulative variance(%) | 75.265 | 88.204 | 95.017 |
权重 Weight | 0.792 | 0.136 | 0.072 |
处理 Treatment | F1 | F2 | F3 | F | 综合排名 Integ- rated rank |
---|---|---|---|---|---|
CK | -2.391 | 2.068 | 0.268 | -1.294 | 5 |
J1 | 1.725 | -0.955 | -0.600 | 1.022 | 3 |
J2 | 3.816 | 0.022 | 0.489 | 3.535 | 1 |
J3 | 3.120 | 0.798 | -0.743 | 2.871 | 2 |
J4 | -0.550 | -0.568 | 1.629 | -0.262 | 4 |
J5 | -2.746 | -1.537 | -0.329 | -3.098 | 7 |
J6 | -2.974 | 0.173 | -0.713 | -2.775 | 6 |
表4 不同浓度几丁聚糖处理的品质综合评价
Tab.4 Comprehensive quality evaluation of different concentrations of chitosan treatment
处理 Treatment | F1 | F2 | F3 | F | 综合排名 Integ- rated rank |
---|---|---|---|---|---|
CK | -2.391 | 2.068 | 0.268 | -1.294 | 5 |
J1 | 1.725 | -0.955 | -0.600 | 1.022 | 3 |
J2 | 3.816 | 0.022 | 0.489 | 3.535 | 1 |
J3 | 3.120 | 0.798 | -0.743 | 2.871 | 2 |
J4 | -0.550 | -0.568 | 1.629 | -0.262 | 4 |
J5 | -2.746 | -1.537 | -0.329 | -3.098 | 7 |
J6 | -2.974 | 0.173 | -0.713 | -2.775 | 6 |
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