补饲不同剂量竹叶提取物对泌乳期伊犁马血液生化指标的影响

doi:10.6048/j.issn.1001-4330.2022.11.022

新疆农业科学 ›› 2022, Vol. 59 ›› Issue (11): 2772-2778.DOI: 10.6048/j.issn.1001-4330.2022.11.022

• 马运动生理与健康养殖 • 上一篇下一篇

补饲不同剂量竹叶提取物对泌乳期伊犁马血液生化指标的影响

吴睿宸¹(), 姚新奎¹^,²^,³, 孟军¹^,²^,³(), 曾亚琦¹^,²^,³, 王川坤¹^,², 阿拉法特·艾合买提¹, 努尔塔依·库尔班塔依¹

1.新疆农业大学动物科学学院,乌鲁木齐 830052
2.新疆农业大学马产业研究院,乌鲁木齐 830052
3.新疆马繁育与运动生理重点实验室,乌鲁木齐 830052

收稿日期:2022-01-11 出版日期:2022-11-20 发布日期:2022-12-28
通信作者: 孟军
作者简介:吴睿宸(1996-),男,重庆彭水人,硕士研究生,研究方向为动物生产学,(E-mail)619673328@qq.com
基金资助:
自治区创新环境(人才、基地)建设专项(PT2220);新疆维吾尔自治区重大科技专项(2022A02007-1)

Effects of Different Dosages of Bamboo Leaf Extract on Blood Biochemical Indexes of Yili Horse during Lactation

WU Ruichen¹(), YAO Xinkui¹^,²^,³, MENG Jun¹^,²^,³(), ZENG Yaqi¹^,²^,³, WANG Chuankun¹^,², Arafat Aihemati¹, Nurtayi Kurbantai¹

1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
2. Institute of Horse Industry, Xinjiang Agricultural University, Urumqi 830052, China
3. Xinjiang Key Laboratory of Equine Breeding and Exercise Physiology, Urumqi 830052, China

Received:2022-01-11 Online:2022-11-20 Published:2022-12-28
Correspondence author: MENG Jun
Supported by:
The Innovation Environment (Talent, Base) Construction Project of Xinjiang Uygur Autonomous Region(PT2220);Major science and technology projects of Xinjiang Uygur Autonomous Region(2022A02007-1)

摘要/Abstract

摘要：

【目的】研究补喂不同剂量竹叶提取物对泌乳期伊犁马抗氧化指标、血清蛋白、免疫指标和激素指标的影响,为分析竹叶提取物能够作为调节伊犁马母马抗氧化、免疫性能及激素的新型无害添加剂提供理论依据。【方法】选取伊犁哈萨克自治州昭苏马场同一草场放牧条件下体况相近的24匹母马作为研究对象,随机分为4组,每组6匹马;对照组不添加竹叶提取物,试验Ⅰ、Ⅱ、Ⅲ组分别添加10、20、30 g/d,饲喂周期为60 d,在第0、15、30、45、60 d通过采集母马的颈静脉血液分析不同补饲梯度血液生化指标的差异。【结果】(1)血液中抗氧化因子:试验Ⅲ组MDA的浓度极显著低于对照组及试验Ⅰ组(P<0.01)。(2)血清蛋白:试验Ⅱ组TP浓度显著低于试验Ⅰ、Ⅲ组(P<0.05),极显著低于对照组(P<0.01);试验Ⅰ组ALB浓度显著低于对照组(P<0.05),极显著低于试验Ⅲ组(P<0.01);试验Ⅱ组GLB浓度极显著低于对照组、试验Ⅰ组(P<0.01);试验Ⅰ组A/G极显著低于试验Ⅱ、Ⅲ组(P<0.01)。(3)血液免疫因子:试验Ⅱ组IL-1β、TNF-α浓度极显著高于对照组(P<0.01),显著高于试验Ⅰ组(P<0.05)。(4)血液激素:试验Ⅱ组PRL浓度极显著高于对照组(P<0.01),显著高于试验Ⅰ组(P<0.05)。【结论】补饲竹叶提取物能够提高泌乳期伊犁马的抗氧化性能。补饲竹叶提取物能够调节激素含量变化,改善泌乳期伊犁马乳汁分泌,提高其产奶性能。

关键词: 竹叶提取物; 泌乳期伊犁马; 抗氧化; 免疫; 激素

Abstract:

【Objective】 This experiment aims to investigate the effects of different doses of bamboo leaf extract on the antioxidant index, serum protein, immune index and hormone index of Yili horse during lactation in order to provide a theoretical basis for mastering the bamboo leaf extract that can be used as a new harmless additive to regulate the equine anti-oxidation, immune performance and hormones. 【Methods】 Twenty-four mares with similar body condition under grazing conditions on the same pasture in Zhaosu Stud-Farm of Yili Kazakh Autonomous Prefecture were selected as the research objects, and they were randomly divided into 4 groups with 6 horses in each group; The control group was not added with bamboo leaf extract, experiment I. Groups II and III were supplemented with bamboo leaf extract dosages 10 g/d, 20 g/d, and 30 g/d, respectively, and the feeding cycles were 60 days. On the 0 th, 15 th, 30 th, 45 th, and 60 th day, the mare's jugular vein blood was collected for analysis to study the effects of different supplementary feeding gradients on their blood biochemical indexes. 【Results】 (1) Antioxidant factors in blood: the concentration of MDA in test group III was significantly lower than that of control group and test group I (P<0.01). (2) Serum protein: The TP concentration of test group II was significantly lower than that of test groups I and III (P<0.05), and it was extremely significantly lower than that of control group (P<0.01); the concentration of ALB in test group I was significantly lower than that of control group (P<0.05), extremely significantly lower than test group III (P<0.01); test group II GLB concentration was extremely significantly lower than the control group, test group I (P<0.01); test group I A/G was extremely significantly lower than test II, Group III (P<0.01). (3) Blood immune factors: The concentration of IL-1β and TNF-α in the test group II was extremely significantly higher than that of the control group (P<0.01), and significantly higher than that in the test group I (P<0.05). (4) Blood hormones: The concentration of PRL in the test II group was extremely significantly higher than that in the control group (P<0.01), and significantly higher than that in the test I group (P<0.05). 【Conclusion】 Under the conditions of this experiment, supplementary feeding with bamboo leaf extract can improve the antioxidant performance of Yili horses during lactation and regulate changes in hormone content, improve milk secretion during lactation, and improve its milk production performance.

Key words: bamboo leaf extract; Yili horse during lactation; antioxidant; immunity; hormone

中图分类号:

S829.4

吴睿宸, 姚新奎, 孟军, 曾亚琦, 王川坤, 阿拉法特·艾合买提, 努尔塔依·库尔班塔依. 补饲不同剂量竹叶提取物对泌乳期伊犁马血液生化指标的影响[J]. 新疆农业科学, 2022, 59(11): 2772-2778.

WU Ruichen, YAO Xinkui, MENG Jun, ZENG Yaqi, WANG Chuankun, Arafat Aihemati, Nurtayi Kurbantai. Effects of Different Dosages of Bamboo Leaf Extract on Blood Biochemical Indexes of Yili Horse during Lactation[J]. Xinjiang Agricultural Sciences, 2022, 59(11): 2772-2778.

图/表 4

参考文献 29

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项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	P值P-value
项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	Trt	Date	Trt×Date
丙二醛 MDA (nmol/ml)	2.986^A	3.029^A	2.759^AB	2.409^B	0.132	0.005	0.005	0.233
羟自由基 -OH (U/ml)	574.81	599.336	518.152	528.126	34.195	0.307	0.053	0.605
超氧化物岐化酶 SOD (U/ml)	75.796	78.255	74.031	69.985	3.352	0.377	0.003	0.814

项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	P值P-value
项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	Trt	Date	Trt×Date
丙二醛 MDA (nmol/ml)	2.986^A	3.029^A	2.759^AB	2.409^B	0.132	0.005	0.005	0.233
羟自由基 -OH (U/ml)	574.81	599.336	518.152	528.126	34.195	0.307	0.053	0.605
超氧化物岐化酶 SOD (U/ml)	75.796	78.255	74.031	69.985	3.352	0.377	0.003	0.814

项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	P值P-value
项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	Trt	Date	Trt×Date
总蛋白 TP(g/L)	76.218^Aa	74.175^ABa	70.625^Bb	74.196^ABa	1.049	0.003	0	0.39
白蛋白 ALB(g/L)	22.124^ABab	20.706^Bc	21.457^ABbc	22.741^Aa	0.451	0.016	0.005	0.968
球蛋白 GLB(g/L)	54.093^A	53.469^A	49.168^B	51.455^AB	1.018	0.004	0.009	0.545
白蛋白球蛋白比 A/G	0.414^AB	0.391^B	0.441^A	0.445^A	0.012	0.009	0.43	0.979

项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	P值P-value
项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	Trt	Date	Trt×Date
总蛋白 TP(g/L)	76.218^Aa	74.175^ABa	70.625^Bb	74.196^ABa	1.049	0.003	0	0.39
白蛋白 ALB(g/L)	22.124^ABab	20.706^Bc	21.457^ABbc	22.741^Aa	0.451	0.016	0.005	0.968
球蛋白 GLB(g/L)	54.093^A	53.469^A	49.168^B	51.455^AB	1.018	0.004	0.009	0.545
白蛋白球蛋白比 A/G	0.414^AB	0.391^B	0.441^A	0.445^A	0.012	0.009	0.43	0.979

项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	P值P-value
项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	Trt	Date	Trt×Date
免疫球蛋白G IgG (g/L)	19.232	19.527	18.706	18.638	0.443	0.448	0.098	0.54
免疫球蛋白M IgM (g/L)	1.266	1.292	1.231	1.200	0.043	0.474	0.045	0.565
白细胞介素 IL-1β(pg/ml)	19.430^Bb	20.421^ABb	25.656^Aa	22.043^ABab	1.618	0.043	0.758	0.999
肿瘤坏死因子 TNF-α(pg/ml)	46.979^Bb	48.609^ABb	59.546^Aa	51.853^ABab	3.318	0.045	0.815	0.999

补饲不同剂量竹叶提取物对泌乳期伊犁马血液生化指标的影响

Effects of Different Dosages of Bamboo Leaf Extract on Blood Biochemical Indexes of Yili Horse during Lactation

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项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	P值P-value
项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	Trt	Date	Trt×Date
甲状腺素 T4(ng/ml)	29.415	28.982	29.653	29.759	0.717	0.882	0.001	0.383
孕酮 P(ng/ml)	10.601	8.418	9.754	10.749	1.157	0.491	0.668	0.829
雌二醇 E2 (pg/ml)	209.404	323.876	361.104	272.865	57.8	0.284	0.366	0.737
催乳素 PRL (ug/ml)	0.537^Bb	0.572^ABb	0.730^Aa	0.620^ABab	0.042	0.011	0.699	0.995

项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	P值P-value
项目 Items	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial group Ⅱ	试验Ⅲ组 Trial group Ⅲ	标准误 SE	Trt	Date	Trt×Date
甲状腺素 T4(ng/ml)	29.415	28.982	29.653	29.759	0.717	0.882	0.001	0.383
孕酮 P(ng/ml)	10.601	8.418	9.754	10.749	1.157	0.491	0.668	0.829
雌二醇 E2 (pg/ml)	209.404	323.876	361.104	272.865	57.8	0.284	0.366	0.737
催乳素 PRL (ug/ml)	0.537^Bb	0.572^ABb	0.730^Aa	0.620^ABab	0.042	0.011	0.699	0.995