伊犁马LPL基因多态性及其与肉质性状关联性分析

doi:10.6048/j.issn.1001-4330.2022.11.021

新疆农业科学 ›› 2022, Vol. 59 ›› Issue (11): 2765-2771.DOI: 10.6048/j.issn.1001-4330.2022.11.021

• 马运动生理与健康养殖 • 上一篇下一篇

伊犁马LPL基因多态性及其与肉质性状关联性分析

王建文¹^,²(), 丁玉泽¹, 李璐伶¹, 姚新奎¹^,², 曾亚琦¹^,², 闫睛¹, 任万路¹, 王川坤¹, 孟军¹^,²()

1.新疆农业大学动物科学学院,乌鲁木齐 830052
2.新疆马繁育与运动生理重点实验室/新疆农业大学马产业研究院,乌鲁木齐 830052

收稿日期:2022-01-11 出版日期:2022-11-20 发布日期:2022-12-28
通信作者: 孟军
作者简介:王建文(1988-),男,湖北天门人,博士,副教授,研究方向为马类科学,(E-mail)275139618@qq.com
基金资助:
新疆维吾尔自治区天池博士计划;新疆维吾尔自治区重大科技专项(2022A02007-1);自治区创新环境(人才、基地)建设专项(PT2220);新疆农业大学大学生创新项目(dxscx2020140)

Polymorphism of LPL Gene and Its Relationship with Meat Quality Traits in Yili Horse

WANG Jianwen¹^,²(), DING Yuze¹, LI Luling¹, YAO Xinkui¹^,², ZENG Yaqi¹^,², YAN Jing¹, REN Wanlu¹, WANG Chuankun¹, MENG Jun¹^,²()

1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
2. Xinjiang Key Laboratory of Equine Breeding and Exercise Physiology / Research Institute of Horse Industry,Xinjiang Agricultural University,Urumqi 830052,China

Received:2022-01-11 Online:2022-11-20 Published:2022-12-28
Correspondence author: MENG Jun
Supported by:
Dr. Tianchi Project of Xinjiang Uygur Autonomous Region;Major science and technology projects of Xinjiang Uygur Autonomous Region(2022A02007-1);The Innovation Environment (Talent, Base) Construction Project of Xinjiang Uygur Autonomous Region(PT2220);Undergraduate Innovation Project of Xinjiang Agricultural University(dxscx2020140)

摘要/Abstract

摘要：

【目的】研究伊犁马脂蛋白脂酶(lipoprotein lipase,LPL)基因多态性及其对肉质性状的影响,为专门化肉用马选种提供技术支撑。【方法】以41匹成年伊犁马为研究对象,采集颈静脉血液样本及其三角肌、背阔肌、臂三头肌、腹外斜肌和臀中肌5个部位肌肉样本,提取伊犁马DNA样本并测定肉质性状,利用PCR测序法筛选LPL基因突变位点并进行多态性分析,结合伊犁马肉质性状数据与LPL基因多态性进行关联性分析。【结果】所选马匹中共检测出2个突变位点(g.715T>C和g.12074C>A),均为内含子突变,2个位点均只检测出2种基因型,为低度多态位点。g.715T>C位点TT基因型马匹背阔肌和臀中肌失水率显著高于TC基因型马匹(P<0.05),背阔肌剪切力极显著大于TC基因型马匹(P<0.01),腹外斜肌剪切力极显著小于TC基因型马匹(P<0.05),三角肌和臀中肌剪切力显著大于TC基因型马匹(P<0.05);g.12074C>A突变位点CC基因型马匹臂三头肌和臀中肌失水率极显著低于CA基因型马匹(P<0.01),背阔肌和腹外斜肌失水率显著低于CA基因型马匹(P<0.05),臂三头肌熟肉率极显著高于CA基因型马匹(P<0.01),三角肌和腹外斜肌熟肉率与CA基因型马匹呈显著差异(P<0.05),臂三头肌和腹外斜肌剪切力极显著高于CA基因型马匹(P<0.01)。【结论】LPL基因g.715T>C和g.12074C>A位点是影响伊犁马肉质性状的潜在位点。

关键词: 伊犁马; LPL基因; 多态性; 肉质性状

Abstract:

【Objective】 To investigate the polymorphism of lipoprotein lipase gene (LPL)and its effect on meat quality traits in Yili horses, and to provide technical support for the selection of specialized meat horses. 【Methods】 In this study, blood samples were collected from 41 adult Yili horses from the jugular vein and five muscle parts: deltoid muscle, latissimus dorsi, arm triceps, external oblique muscle and gluteal muscle to extract Yili horse DNA samples and determine meat quality traits. PCR sequencing was used to screen LPL gene mutation loci and perform polymorphism analysis, combined with data on meat quality traits of Yili horse and LPL gene polymorphism for association analysis. 【Results】 Two mutant loci (g.715T>C and g.12074C>A) were detected in the selected horses, both of which were intronic mutations. Only 2 genotypes were detected for both loci, which were low polymorphic loci. g.715T>C locus TT genotype horses had significantly higher dorsalis latissimus and gluteal musclewater loss rate than TC genotype horses(P<0.05), significantly greater dorsalis latissimus shear force than TC genotype horses(P<0.01), significantly less external oblique muscleshear force than TC genotype horses(P<0.05), and significantly greater deltoid muscle and gluteal muscleshear force than TC genotype horses(P<0.05). g. 12074C > A mutant horses with the CC genotype had a significantly lower water loss rate in the arm triceps and gluteal muscle(P<0.01), a significantly lower water loss rate in the latissimus dorsi and oblique abdominis than in the CA genotype(P<0.05), a significantly higher cooked meat rate in the arm triceps than in the CA genotype(P<0.01), a significantly difference of cooked meat rate was found in the deltoid muscle and oblique abdominis than in the CA genotype(P<0.05), and a significantly higher rate of shear force in the arm triceps and oblique abdominis than in the CA genotype(P<0.01).【Conclusion】 The g.715T>C and g.12074C>A loci of the LPL gene may be potential loci for meat quality traits in Yili horses.

Key words: Yili horse; LPL gene; polymorphism; meat quality traits

中图分类号:

S821.9⁺1

王建文, 丁玉泽, 李璐伶, 姚新奎, 曾亚琦, 闫睛, 任万路, 王川坤, 孟军. 伊犁马LPL基因多态性及其与肉质性状关联性分析[J]. 新疆农业科学, 2022, 59(11): 2765-2771.

WANG Jianwen, DING Yuze, LI Luling, YAO Xinkui, ZENG Yaqi, YAN Jing, REN Wanlu, WANG Chuankun, MENG Jun. Polymorphism of LPL Gene and Its Relationship with Meat Quality Traits in Yili Horse[J]. Xinjiang Agricultural Sciences, 2022, 59(11): 2765-2771.

图/表 7

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名称 Name	引物序列(5’→3’) Sequences(5’→3’)	产物大小 Size(bp)
第1外显子 Exon 1	F:GATTCATTATCCATATCTCCCTTG R:GCAAGAGGAGAAGTTTCCAAAG	664
第3外显子 Exon 3	F:AAAATAGTCTGTAATTCTGCTGCAA R:CACTTAGTTCTCGGTCTCAGCA	785
第4外显子 Exon 4	F:TTCAGATGTATGGAAATTCAGATGT R:GCAGGACTCTCCATGCTCAA	621
第10外显子 Exon 10	F:CTTCTCGCTGGCACAGTTAATC R:TCCTTCCCGTCTTCACAACC	515

名称 Name	引物序列(5’→3’) Sequences(5’→3’)	产物大小 Size(bp)
第1外显子 Exon 1	F:GATTCATTATCCATATCTCCCTTG R:GCAAGAGGAGAAGTTTCCAAAG	664
第3外显子 Exon 3	F:AAAATAGTCTGTAATTCTGCTGCAA R:CACTTAGTTCTCGGTCTCAGCA	785
第4外显子 Exon 4	F:TTCAGATGTATGGAAATTCAGATGT R:GCAGGACTCTCCATGCTCAA	621
第10外显子 Exon 10	F:CTTCTCGCTGGCACAGTTAATC R:TCCTTCCCGTCTTCACAACC	515

组分 Component	浓度 Concentration	体积 Volume(μL)
模板DNA Template DNA	20~50 ng/μL	2
上游引物 Upstream Primer	10 μM	1
下游引物 Downstream primer	10 μM	1
dNTP	10 mM	1
Taq Buffer(含Mg^2＋) Taq Buffer (With Mg^2＋)	10×	2.5
Taq Plus DNA聚合酶 Taq Plus DNA Polymerase	5 U/μL	0.2
ddH₂O		17.3

组分 Component	浓度 Concentration	体积 Volume(μL)
模板DNA Template DNA	20~50 ng/μL	2
上游引物 Upstream Primer	10 μM	1
下游引物 Downstream primer	10 μM	1
dNTP	10 mM	1
Taq Buffer(含Mg^2＋) Taq Buffer (With Mg^2＋)	10×	2.5
Taq Plus DNA聚合酶 Taq Plus DNA Polymerase	5 U/μL	0.2
ddH₂O		17.3

SNPs	样本数 Samples(匹)	基因型频率 Genotypefrequency		基因频率 Genefrequency		χ²
g.715T>C	41	TT 0.878 0 (36)	TC 0.122 0 (5)	T 0.939 0	C 0.061 0	0.172 9
g.12074C>A	41	CC 0.731 7(30)	CA 0.268 3(11)	C 0.865 9	A 0.134 1	0.984 1

伊犁马LPL基因多态性及其与肉质性状关联性分析

Polymorphism of LPL Gene and Its Relationship with Meat Quality Traits in Yili Horse

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参考文献 28

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Metrics

SNPs	纯合度 Ho	杂合度 He	有效等位基因数 Ne	多态信息含量 PIC	HWE P值 HWE P value
g.715T>C	0.885 5	0.114 5	1.129 3	0.108 0	0.677 6
g.12074C>A	0.767 7	0.232 3	1.302 6	0.205 3	0.321 2

SNPs	部位 Parts	基因型 Genotype	失水率 Water loss rate (％)	熟肉率 Cooked meat rate (％)	剪切力 Shear force (N)
g.715T>C	三角肌	TT(36) TC(5) P	8.35±0.75 8.41±1.06 0.860	55.74±1.52 54.97±2.25 0.323	117.73±10.76 101.25±38.04 0.036
	背阔肌	TT(36) TC(5) P	8.60±1.11 7.42±1.45 0.038	55.23±1.23 54.93±2.26 0.653	100.39±11.57 74.15±26.47 0.000
	臂三头肌	TT(36) TC(5) P	7.72±1.05 9.28±1.88 0.008	55.88±1.19 56.19±2.49 0.797	124.60±13.88 125.94±23.74 0.854
	腹外斜肌	TT(36) TC(5) P	8.38±0.81 7.77±1.44 0.402	58.89±1.77 58.48±2.69 0.757	77.23±16.56 106.11±27.50 0.002
	臀中肌	TT(36) TC(5) P	10.47±1.86 8.24±1.12 0.013	56.44±1.15 56.36±1.68 0.901	101.67±18.21 79.06±21.18 0.015
g.12074C>A	三角肌	CC(30) CA(11) P	8.24±0.79 8.66±0.70 0.116	55.99±1.42 54.72±1.80 0.020	117.68±11.54 110.35±25.96 0.385
	背阔肌	CC(30) CA(11) P	8.19±0.86 9.18±1.68 0.017	55.23±1.44 55.07±1.19 0.741	99.12±12.07 91.94±24.24 0.213
	臂三头肌	CC(30) CA(11) P	7.51±1.04 8.98±1.24 0.003	56.33±1.26 54.79±1.00 0.001	129.88±11.49 110.79±14.98 0.002
	腹外斜肌	CC(30) CA(11) P	8.12±0.92 8.81±0.66 0.013	58.45±1.49 59.91±2.42 0.025	89.09±16.70 58.00±6.44 0.000
	臀中肌	CC(30) CA(11) P	9.32±1.12 12.58±1.63 0.000	56.47±1.14 56.30±1.40 0.678	100.68±19.61 94.08±20.36 0.350

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