大豆籽粒低聚糖及其组分含量鉴定与特异种质筛选

doi:10.6048/j.issn.1001-4330.2022.02.011

摘要/Abstract

摘要：

【目的】 研究大豆低聚糖及其组分含量遗传变异及其相关关系,遴选低聚糖及其组分含量特异种质,为大豆低聚糖含量遗传改良奠定物质基础。【方法】 采用高效液相色谱方法,鉴定336份大豆资源低聚糖及其组分含量,分析其遗传变异及相关性,并筛选高、低含量特异种质。【结果】 供试大豆资源蔗糖、棉籽糖、水苏糖、低聚糖含量平均为4.01%、1.16%、2.60%及7.77%,3种组分中以蔗糖含量最高,变异系数为15.21%、25.51%、19.16%与9.04%,以棉籽糖变异系数最大;低聚糖与蔗糖、棉籽糖、水苏糖间存在极显著正相关,蔗糖与棉籽糖间存在极显著正相关,而水苏糖与蔗糖、棉籽糖间存在极显著负相关;基于供试品种低聚糖及其组分含量,将其分为3类,并筛选出各组分高、低含量特异种质各5份,其中包括高含量种质呼交282、青杂豆、合丰44等,低含量种质宾县黑豆、哈12-4547、绥无腥豆3号等。【结论】 优化了大豆低聚糖及其组分含量检测方法,分离高效稳定;供试群体材料低聚糖及其组分含量存在丰富遗传变异,具有较大选择潜力。

关键词: 大豆; 低聚糖; 高效液相色谱; 种质筛选; 遗传变异

Abstract:

【Objective】 To determine the genetic variation and correlation of soybean oligosaccharides and its component content and select the specific germplasm of oligosaccharides and its component content which will lay the material foundation for the genetic improvement of soybean oligosaccharides content. 【Methods】 In view of this, the contents of oligosaccharides and its components of 336 soybean varieties were evaluated by high performance liquid chromatography method in this study, and the genetic variation and correlations between the components were analyzed, and also the elite germplasms were selected. 【Results】 The results showed that the sucrose, raffinose, stachyose and oligosaccharide contents in the soybeans were 4.01%, 1.16%, 2.60% and 7.77%, respectively, with the sucrose having the highest content. The coefficient of variation (CV%) was 15.21%, 25.51%, 19.16% and 9.04%, respectively, with the raffinose having the highest CV%. Meanwhile, the significant positive correlations between oligosaccharides and sucrose, raffinose, stachyose, the sucrose and raffinose were found; and the significant negative correlations between sucrose and raffinose, stachyose were also detected. Finally, the 336 soybean varieties were divided into three groups based on the contents of oligosaccharides and its components, and some elite germplasms with higher or lower contents were selected, such as the higher content variety Hujiao 282, Qingzadou, Hefeng 44, the lower content variety Binxianheidou, Ha12-4547, Suiwuxingdou 3, etc. 【Conclusion】 The determination method of soybean oligosaccharides and its components is optimized that can result in high efficient and stable separation; there are abundant genetic variations in the oligosaccharides and their contents in the tested population, which has great potential for selection; the selected specific germplasm lays a material foundation for conventional and molecular breeding of soybean oligosaccharides.

Key words: soybean; oligosaccharide; hplc; germplasms selection; heritable variation

中图分类号:

S565.1

李俊茹, 秦宁, 李文龙, 杜汇, 李喜焕, 张彩英. 大豆籽粒低聚糖及其组分含量鉴定与特异种质筛选[J]. 新疆农业科学, 2022, 59(2): 353-360.

LI Junru, QIN Ning, LI Wenlong, DU Hui, LI Xihuan, ZHANG Caiying. Identification of Seed Oligosaccharide and Its Components and Screening for Elite Germplasms in Soybean[J]. Xinjiang Agricultural Sciences, 2022, 59(2): 353-360.

图/表 11

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低聚糖 Oligosaccharide	回归方程 Linear regression equation	决定系数 Coefficient of determination
蔗糖 Sucrose	Y=88 909X+177.6	0.999 7
棉籽糖 Raffinose	Y=70 846X+6445.9	0.993 6
水苏糖 Stachyose	Y=84 945X-6 584.3	0.999 8

低聚糖 Oligosaccharide	回归方程 Linear regression equation	决定系数 Coefficient of determination
蔗糖 Sucrose	Y=88 909X+177.6	0.999 7
棉籽糖 Raffinose	Y=70 846X+6445.9	0.993 6
水苏糖 Stachyose	Y=84 945X-6 584.3	0.999 8

流动相配比 Ratio of mobile phase	蔗糖Sucrose		棉籽糖Raffinose		水苏糖Stachyose
流动相配比 Ratio of mobile phase	保留时间 Retention time	理论塔板数 Theoretical plate	保留时间 Retention time	理论塔板数 Theoretical plate	保留时间 Retention time	理论塔板数 Theoretical plate
65∶35	5.583	5 342.550	7.035	5 975.969	9.085	6 199.417
70∶30	6.143	6 434.377	8.170	7 861.163	11.271	8 115.926
75∶25	8.725	6 758.959	14.315	8 833.943	24.685	9 113.752

流动相配比 Ratio of mobile phase	蔗糖Sucrose		棉籽糖Raffinose		水苏糖Stachyose
流动相配比 Ratio of mobile phase	保留时间 Retention time	理论塔板数 Theoretical plate	保留时间 Retention time	理论塔板数 Theoretical plate	保留时间 Retention time	理论塔板数 Theoretical plate
65∶35	5.583	5 342.550	7.035	5 975.969	9.085	6 199.417
70∶30	6.143	6 434.377	8.170	7 861.163	11.271	8 115.926
75∶25	8.725	6 758.959	14.315	8 833.943	24.685	9 113.752

编号 Number	蔗糖 Sucrose(%)	棉籽糖 Raffinose(%)	水苏糖 Stachyose(%)
1	5.25	0.45	3.13
2	5.26	0.46	3.17
3	5.28	0.46	3.11
4	5.30	0.47	3.11
5	5.31	0.46	3.07
6	5.24	0.46	3.13
均值	5.27	0.46	3.12
标准差	0.03	0.01	0.03
相对标准偏差	0.53	1.72	1.04