瓜类褪绿黄化病毒及其系统进化分析

doi:10.6048/j.issn.1001-4330.2021.05.010

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (5): 866-872.DOI: 10.6048/j.issn.1001-4330.2021.05.010

• 园艺特产·土壤肥料·植物保护 • 上一篇下一篇

瓜类褪绿黄化病毒及其系统进化分析

韩盛¹, 麦麦提艾力·阿卜杜纳斯尔¹, 杨渡¹, 温且木·阿布列孜², 玉山江·麦麦提¹

1.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091;
2.阿勒泰市菜篮子工程办公室,新疆阿勒泰 836500

收稿日期:2020-05-20 出版日期:2021-05-20 发布日期:2021-05-26
通信作者: 玉山江·麦麦提(1978-),男,新疆阿克苏人,副研究员,研究方向为瓜类病害,(E-mail)yusanjan418@163.com麦麦提艾力·阿卜杜纳斯尔(1991-),男,新疆喀什人,博士研究生,研究方向为瓜类病害,(E-mail)maimaitiaili495@sina.com
作者简介:韩盛(1981-),男,河北海兴人,副研究员,研究方向为瓜类病害,(E-mail)108731755@qq.com
基金资助:
新疆维吾尔自治区科技援疆计划“阿泰灵在防治甜瓜病毒病的应用及配套技术研究”(2019E0243)

The Occurrence of Cucurbit chlorotic yellows virus in Lop County in Hotan and Its Phylogenetic Analysis

HAN Sheng¹, Maimaitiaili Abudunasier¹, YANG Du¹, Wenqiemu Abuliezi²,Yushanjiang Maimaiti¹

1. Key Laboratory of Integrated Crop Pest Management in Northwestern Oasis of China,MOARA, Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;
2. Altay Vegetable Basket Engineering Program Office, Altay Xinjiang 836500,China

Received:2020-05-20 Online:2021-05-20 Published:2021-05-26
Correspondence author: Yushanjiang Maimaiti(1978-), Male, from Xinjiang of Aksu, Associate researcher, Research area: Melons diseases, (E-mail)yusanjan418@163.com
Supported by:
S & T Supporting Xinjiang Project of Xinjiang Uygur Autonomous Region “Study on the Application and Supporting Technology of Atelim in the Prevention and Control of Melon Virus Disease” (2019E0243)

摘要/Abstract

摘要： 【目的】检测新疆南疆7个县/市瓜类褪绿黄化病毒(Cucurbit chlorotic yellows virus, CCYV),进行系统发育分析,为新疆南疆CCYV预警和防控提供参考。【方法】于2019年,从中国新疆南疆7个县/市采集带有黄化特征的51份甜瓜叶片样品,RT-PCR进行CCYV的检测,进行特异性片段的克隆、测序和系统发育分析。【结果】中国新疆巴楚县、阿克苏市、莎车县、伽师县、疏勒县、疏附县均未检测到CCYV,而洛浦县的13份样本中,8份检出为CCYV阳性,检出率为61.53%,洛浦县CCYV特异性片段克隆测序获得了685 bp的核酸序列,与GenBank中的CCYV分离物外壳蛋白(Coat protein, CP)的一致性达到100%。所得序列与中国新疆(吐鲁番)、中国其他省、苏丹、日本、塞浦路斯、黎巴嫩的CP基因聚在I组(Group),沙特阿拉伯的分离物聚在Ⅱ组,伊朗的分离物聚在Ⅲ组。【结论】CCYV在中国新疆南疆洛浦县甜瓜产区发生,与中国新疆吐鲁番、中国其他省及周围国家的CCYV分离物亲缘关系很近,且群体遗传变异很小。

关键词: 瓜类褪绿黄化病毒; 和田; 甜瓜; 病毒分布; 病毒进化

Abstract: 【Objective】 To detect Cucurbit chlorotic yellows virus(CCYV)in seven counties/city in Southern Xinjiang and analyze the phylogenetic relationship with a view to providing a reference for CCYV early warning and control in Southern Xinjiang. 【Methods】 In 2019, 51 samples of muskmelon leaves with yellowing symptoms were collected from 7 counties/city in southern Xinjiang and CCYV detection was carried out by RT-PCR for cloning, sequencing and phylogenetic analysis of specific fragments. 【Results】 CCYV was not detected in Bachu County, Aksu City, Shache County, Jiashi County, Shule County and Shufu County, while 8 of 13 samples in Lop County were detected with CCYV, and detection ratio was 61.53%. A 685 bp nucleic acid sequence of CCYV in Lop County was obtained by sequencing, and the consistency with the CCYV isolate shell protein (coat protein, cp) in the GenBank reached 100%. The phylogenetic tree based on CP gene sequence showed that the CP genes of Xinjiang (Turpan), other provinces of China, Sudan, Japan, Cyprus and Lebanon were clustered in group I, Saudi Arabia isolates were clustered in group II, Iran isolates were clustered in group III. 【Conclusion】 CCYV that first occurred in the main melon area of Lop County of southern Xinjiang has close genetic relationships with Turpan, Xinjiang,China, and surrounding countries whose population genetic variation is very low.

Key words: Cucurbit chlorotic yellows virus; Hotan; muskmelon; virus distribution; virus evolution

中图分类号:

S436.5

韩盛, 麦麦提艾力·阿卜杜纳斯尔, 杨渡, 温且木·阿布列孜, 玉山江·麦麦提. 瓜类褪绿黄化病毒及其系统进化分析[J]. 新疆农业科学, 2021, 58(5): 866-872.

HAN Sheng, Maimaitiaili Abudunasier, YANG Du, Wenqiemu Abuliezi,Yushanjiang Maimaiti. The Occurrence of Cucurbit chlorotic yellows virus in Lop County in Hotan and Its Phylogenetic Analysis[J]. Xinjiang Agricultural Sciences, 2021, 58(5): 866-872.

参考文献 20

[1]	Okuda M,Okazaki S,Yamasaki S,et al. Host range and complete genome sequence of Cucurbit chlorotic yellows virus, a new member of the genus Crinivirus[J]. Phytopathology,2010,100(6):560-566.
[2]	Gu QS,Liu YH,Wang YH,et al. First report of Cucurbit chlorotic yellows virus in Cucumber, Melon, and Watermelon in China[J]. Plant Disease,2011,95(1):73.
[3]	古勤生,彭斌,刘珊珊,等. 瓜类新病毒病害(一):瓜类褪绿黄化病[J]. 中国瓜菜,2011,24(3):47.GU Qinsheng,PENG Bin,LIU Shanshan,et al. Diseases of new viruses in cucurbits (1):Cucurbit chlorotic yellows virus[J]. China Cucurbits and Vegetables,2011,24 (3):47.
[4]	Hamed,Menzel,Dafalla,et al. First report of Cucurbit chlorotic yellows virus infecting Muskmelon and Cucumber in Sudan[J]. Plant Disease,2011,(95):1321.
[5]	Abrahamian PE,Sobh H,Abou-Jawdah Y. First report of Cucurbit chlorotic yellows virus on Cucumber in Lebanon[J]. Plant Disease, 2012,95(1):73.
[6]	Bananej K,Menzel W,Kianfar N,et al. First report of Cucurbit chlorotic yellows virus infecting Cucumber, Melon, and Squash in Iran[J]. Plant Disease,2013,97(7):1,005.
[7]	Orfanidou C,Maliogka VI,Katis NI. First report of Cucurbit chlorotic yellows virus in cucumber, melon, and watermelon in Greece[J]. Plant Disease,2014,98(10):1005.
[8]	Amer MA. Serological and molecular characterization of Cucurbit chlorotic yellows virus affecting cucumber plants in Egypt[J]. International Journal of Virology,2015,11(1):1-11.
[9]	Saleh MAA,Al-Shahwan I,Amer MA,et al. First report of Cucurbit chlorotic yellows virus in Cucumber in Saudi Arabia[J]. Plant Disease,2015,99(5):1-2.
[10]	刘珊珊,彭斌,吴会杰,等. 海南省和河南省发生甜瓜褪绿黄化病的分子鉴定[J]. 果树学报,2013,(2):113-115.LIU Shanshan,PENG Bin,WU Huijie,et al. Molecular identification of Cucurbit chlorotic yellows virus in Hainan and Henan provinces[J]. Journal of Fruit Science,2013,(2):113-115.
[11]	彭斌,刘莉铭,刘珊珊,等. 瓜类褪绿黄化病毒新疆分离物基因组分析[J]. 植物病理学报,2017,47(6):730-737.PENG Bin,LIU Liming,LIU Shanshan,et al. The genome of Cucurbit chlorotic yellows virus from Xinjiang autonomous regionof China [J]. Acat Phytopathologica Sinica,2017,47 (6):730-737.
[12]	唐鑫,张德咏,李凡,等. 瓜类褪绿黄化病毒(Cucurbit chlorotic yellows virus)在湖南省的首次报道及其流行动态研究[J]. 植物病理学报,2017,(4):144-147.TANG Xin,ZHANG Deyong,LI Fan,et al. The first report and the epidemical dynamic of the Cucurbit chlorotic yellows virus(CCYV) in Hunan province[J]. Acat Phytopathologica Sinica,2017,(4):144-147.
[13]	杨世安,李战彪,秦碧,等. 广西三种甜瓜病毒分离物的分子检测与鉴定[J]. 植物保护,2017,(3):83-89.YANG Shian,LI Zhanbiao,QIN Bi,et al. Molecular detection and identification of three melon virus isolates in Guangxi[J]. Plant Protection,2017,(3):83-89.
[14]	Li J,Liang X,Wang X,et al. Direct evidence for the semipersistent transmission of Cucurbit chlorotic yellows virus by a whitefly vector[J]. Scientific Reports,2016,6(1):1-8.
[15]	刘珊珊,严蕾艳,王毓洪,等. 甜瓜褪绿黄化病的发生与传毒介体烟粉虱群体数量关系的调查[J]. 中国瓜菜,2016,29(11):7-10.LIU Shanshan, YAN Leiyan, WANG Yuhong, et al. Investigation on the relationship between the occurrence of Cucurbit chlorotic yellows virus and Bemisia tabaci populations[J]. China Cucurbits and Vegetables,2016, 29 (11):7-10.
[16]	Tang X , Shi X , Zhang D , et al. Detection and epidemic dynamic of ToCV and CCYV with Bemisia tabaci and weed in Hainan of China [J]. Virology Journal, 2017, 14(1):169.
[17]	曹骞,李晶,买热木古丽·克依木,等. 新疆地区烟粉虱生物型的区域分布及其携带的番茄黄化曲叶病毒检测[J]. 昆虫学报,2013,56(6):652-664.CAO Qian, LI Jing, Mairemu Guli·Yimu, et al. Regional distribution of Bemisia tabaci biotypes and detection of tomato yellow leaf curl virus in Xinjiang[J]. Acta Entomologica Sinica,2013,56(6):652-664.
[18]	潘卫萍,张以和,吉艳玲. 吐鲁番首次发生甜瓜褪绿黄化病毒病[J]. 蔬菜,2017,(2):60-61.PAN Weiping,ZHANG Yihe,JI Yanling. The first occurrence of Cucurbit chloroticyellows virus in Turpan[J]. Vegetables,2017,(2):60-61.
[19]	臧连毅,孙晓辉,苏文敏,等. 瓜类褪绿黄化病毒山东分离物全基因组序列扩增及分析[J].植物保护学报,2019,46(6):1195-1202.ZANG Lianyi,SUN Xiaohui,SU Wenmin,et al. Amplification and analysis of whole genome sequence of Cucurbit chloroticyellows virus from Shandong Province,China[J]. Journal of Plant Protection,2019,46(6):1195-1202.
[20]	段晓东,马丽娟,姚正培,等. 新疆地区烟粉虱类群mtDNA COI基因序列分析[J]. 生物安全学报,2011,(1):60-65.DUAN Xiaodong,MA Lijuan,YAO Zhengpei,et al. Sequence analysis of mtDNA COI gene of Bemisia tabaci populations in Xinjiang[J]. Journal of Biosafety,2011,(1):60-65.

瓜类褪绿黄化病毒及其系统进化分析

The Occurrence of Cucurbit chlorotic yellows virus in Lop County in Hotan and Its Phylogenetic Analysis

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

参考文献 20

相关文章 15

编辑推荐

Metrics

[1]	郑贺云, 姚军, 李超, 张翠环, 耿新丽. 不同贮藏温度对甜瓜品质的影响及预测模型建立[J]. 新疆农业科学, 2023, 60(8): 1950-1957.
[2]	姚军, 郑贺云, 张翠环, 再吐娜·买买提, 耿新丽. LED持续光照处理对甜瓜贮藏特性及糖代谢的影响[J]. 新疆农业科学, 2023, 60(7): 1689-1697.
[3]	李自芹, 李文绮, 贾文婷, 李宇辉, 刘成江. 氯化钙与1-MCP对西州蜜甜瓜采后贮藏品质的影响[J]. 新疆农业科学, 2023, 60(7): 1698-1704.
[4]	刘阳, 白羽嘉, 张郑啸, 楚晨俐, 王甜甜, 冯作山. 甜瓜抗链格孢侵染病程相关蛋白及苯丙烷代谢酶的变化[J]. 新疆农业科学, 2023, 60(7): 1780-1789.
[5]	贾斌鑫, 杨世英, 王艳, 杨朋朋, 何伟忠, 王成, 刘峰娟, 范盈盈. 不同品种厚皮甜瓜采后糖含量及糖代谢酶活性比较[J]. 新疆农业科学, 2023, 60(6): 1476-1484.
[6]	田敬宇, 高雁, 高兴旺, 曾军, 赵鹏安, 苏比努尔·居来提. 哈密瓜种植对根际土壤细菌群落组成和多样性的影响[J]. 新疆农业科学, 2023, 60(5): 1253-1262.
[7]	杨建超, 徐诚, 杨平, 杨鸿基, 张凯浩, 马新超, 高亚宁, 陈有福, 付湘, 轩正英. 氮素指数施肥对设施薄皮甜瓜养分承载及氮吸收利用的影响[J]. 新疆农业科学, 2023, 60(3): 590-600.
[8]	郭阳, 郭俊先, 史勇, 刘丽, 方文艳, 刘彦岑. 特征波长筛选结合PCA-LSSVM对甜瓜叶片SPAD值的预测[J]. 新疆农业科学, 2023, 60(3): 616-623.
[9]	张浩, 刘熠, 周勃, 黄远, 符小发, 马跃, 吴海波, 胡国智, 毛建才, 陈积豪, 梁其干, 王敏. 氮素运筹对甜瓜芳香物质的影响[J]. 新疆农业科学, 2023, 60(12): 2993-3007.
[10]	杨志毅, 王惠林, 王瑞, 王超楠, 徐宝林, 邱火箭. 六种铜制剂对主要甜瓜生长指标、产量和品质的影响[J]. 新疆农业科学, 2023, 60(12): 3008-3017.
[11]	杨世英, 马玉娥, 贾斌鑫, 华震宇, 沈琦, 刘峰娟, 王成. 油菜素内酯对甜瓜农药残留的降解作用分析[J]. 新疆农业科学, 2023, 60(10): 2541-2550.
[12]	才羿, 戴冬洋, 谭海, 王迪, 杨芬, 王岭, 盛云燕. 甜瓜AMS基因结构分析及基因编辑载体构建[J]. 新疆农业科学, 2023, 60(1): 61-68.
[13]	熊韬, 闫淼, 王豪杰, 毛建才, 王江涛, 胡国智. 盐碱胁迫对甜瓜种子萌发及幼苗生长发育的影响[J]. 新疆农业科学, 2022, 59(8): 1965-1974.
[14]	才仁加甫, 曹彪, 白云岗, 刘旭辉, 余其鹰, 刘敏杰. 和田河沙漠段生态输水植被恢复遥感评价和植被变化驱动因素分析[J]. 新疆农业科学, 2022, 59(8): 2041-2050.
[15]	何宗龙, 侯晨曦, 洪文娟, 姚力丹, 决肯•阿尼瓦什. 和田多胎红羊屠宰性能及6月龄羊肉成分分析[J]. 新疆农业科学, 2022, 59(8): 2073-2080.