甜瓜抗链格孢侵染病程相关蛋白及苯丙烷代谢酶的变化

doi:10.6048/j.issn.1001-4330.2023.07.026

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (7): 1780-1789.DOI: 10.6048/j.issn.1001-4330.2023.07.026

甜瓜抗链格孢侵染病程相关蛋白及苯丙烷代谢酶的变化

刘阳¹^,²(), 白羽嘉¹^,²(), 张郑啸¹^,², 楚晨俐¹, 王甜甜¹, 冯作山¹^,²()

1.新疆农业大学食品科学与药学学院,乌鲁木齐 830052
2.新疆果品采后科学技术重点实验室,乌鲁木齐 830052

收稿日期:2022-01-20 出版日期:2023-07-20 发布日期:2023-07-11
通信作者: 冯作山(1963-),男,新疆人,教授,博士,硕士生/博士生导师,研究方向为农产品加工与综合利用,(E-mail)fengzuoshan@126.com；
白羽嘉(1984-),男,新疆人,副教授,博士,硕士生导师,研究方向为农产品加工与综合利用、作物遗传育种、果品采后生物学与物流保鲜,(E-mail)saintbyj@126.com
作者简介:刘阳(1998-),女,新疆人,硕士研究生,研究方向为农产品加工与综合利用,(E-mail)ly98128@163.com
基金资助:
国家自然科学基金项目(31960489)

Changes of related proteins and phenylpropane metabolic enzymes in melon infected by Alternaria alternata

LIU Yang¹^,²(), BAI Yujia¹^,²(), ZHANG Zhengxiao¹^,², CHU Chenli¹, WANG Tiantian¹, FENG Zuoshan¹^,²()

1. College of Food Sciences and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China
2. Xinjiang Key Laboratory for Postharvest Science and Technology of Fruits, Urumqi 830052, China

Received:2022-01-20 Online:2023-07-20 Published:2023-07-11
Correspondence author: FENG Zuoshan (1963-), male, professor, research field:processing and comprehensive utilization of agricultural products,(E-mail)fengzuoshan@126.com；
BAI Yujia (1984-), male, professor, research field:processing and comprehensive utilization of agricultural products,crop genetics and breeding,postharvest biology and logistics preservation of fruits,(E-mail)saintbyj@126.com
Supported by:
National Natural Science Foundation of China "Genome Sequencing of Alternaria. alternata and Pathogenic Mechanism of Postharvest Melon Fruit Infection"(31960489)

摘要/Abstract

摘要：

【目的】分析常温贮藏期间(25℃)不同甜瓜果皮与果肉组织病程相关蛋白和苯丙烷代谢相关酶的差异,探究甜瓜在常温贮藏下的内源抗病性。【方法】甜瓜果实损伤接种链格孢,贮藏期间分离甜瓜果实外果皮、内果皮和果肉组织,测定不同贮藏天数时甜瓜不同组织的病程相关蛋白和苯丙烷代谢相关酶,分析其不同组织抗病能力的差异性,分析不同贮藏温度对甜瓜抗病性的影响。【结果】贮藏期间伽师瓜的病斑直径小于86-1甜瓜,染菌后16 d,86-1甜瓜的果皮和果肉病斑分别是伽师瓜的1.55倍和1.38倍;在此期间甜瓜内几丁质酶(Chitinase,CHT)、β-1,3-葡聚糖酶(β-1,3-Glucan,GLU)、甜瓜4-香豆酰辅酶A连接酶(Coumaroyl-CoA,4CL)、苯丙氨酸解氨酶(L-Phenylalanin Ammo-Nialyase,PAL)、肉桂酸-4-羟化酶(Cinnamic Acid 4-Hydroxylase,C4H)活性呈先升高后下降趋势,伽师瓜酶活性高于86-1甜瓜,两种甜瓜的酶活性均为外果皮>内果皮>果肉组织,伽师瓜比86-1甜瓜更快到达酶活性高峰。【结论】损伤接种链格孢温度对甜瓜果实病程相关蛋白和苯丙烷代谢酶的变化趋势无明显影响,酶活性均呈现先升高后下降的趋势;伽师瓜优于86-1甜瓜,果皮抗病能力强于果肉,伽师瓜比86-1甜瓜更积极抵御链格孢的侵染。

关键词: 链格孢菌; 甜瓜; 病程相关蛋白; 苯丙烷代谢

Abstract:

【Objective】 To analyze the differences of related proteins and enzymes involved in phenylpropanoid metabolism in pathogenesis from diverse melons’ peel and pulp tissues, which are stored in room temperature (25℃) and explore the endogenous disease resistance of melons stored at room temperature.【Methods】 Melon was were inoculated with Alternaria alternata, and their epricarp, endocarp and pulp tissues were separated during the storage. The pathogenesis related proteins and enzymes involved in phenylpropanoid metabolism in different tissues of muskmelons were measured in different storage days,and the disease resistance of different tissues of melon were analyzed. The indicators in these issues were measured every other day to deduce their the disease resistance. Compared with previous researches, the effects of different storage temperatures on disease resistance of muskmelon were figured out as well.【Results】 During storage, the lesion diameter of Jiashigua was smaller than 86-1 melon’s, the lesion size in the peel and pulp of 86-1 melons reached 1.55 and 1.375 times bigger than Jiashigua’s on the 16th day. Activities of Chitinase (CHT) and β-1,3-glucanase (GLU) in melon showed a trend of increasing first and then decreasing, and the enzyme activity of Jiashigua was higher than 86-1 melon’s, exocarp was higher than endocarp and pulp tissue. Compared to 86-1 melon, Jiashigua reached the peak of enzyme activity in a shorter time.【Conclusion】 It is found that temperature has no obvious effect on the change trend of the pathogenesis related proteins and enzymes involved in phenylpropanoid metabolism in melons, and enzyme activities all show a trend of increasing at first and then decreasing; In terms of disease resistance, Jiashigua is superior to 86-1 melon. In dealing with the infection of Alternaria alternata, peels have stronger disease resistance than pulps do, so Jiashigua is more active to resist than 86-1 melon facing the infection of Alternaria alternata.

Key words: Alternaria alternata; muskmelon; pathogenesis related proteins; phenylpropanoid metabolism

刘阳, 白羽嘉, 张郑啸, 楚晨俐, 王甜甜, 冯作山. 甜瓜抗链格孢侵染病程相关蛋白及苯丙烷代谢酶的变化[J]. 新疆农业科学, 2023, 60(7): 1780-1789.

LIU Yang, BAI Yujia, ZHANG Zhengxiao, CHU Chenli, WANG Tiantian, FENG Zuoshan. Changes of related proteins and phenylpropane metabolic enzymes in melon infected by Alternaria alternata[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1780-1789.

图/表 17

图1 链格孢侵染后甜瓜病斑直径的变化

Fig.1 Change in lesion diameter in two melon by A. alternaria

图2 不同天数甜瓜病斑注:a(伽师瓜6 d)、b(86-1甜瓜6 d)、c(伽师瓜10 d)、d(86-1甜瓜10 d)、e(伽师瓜16 d)、f(86-1甜瓜16 d)

Fig.2 Change in disease spot size of two melon in different days Note:a (Jiashigua 6 d), b(86-1 melon 6 d), c(Jiashigua 10 d), d(86-1 melon 10 d), e(Jiashigua 16 d), f(86-1 melon 16 d)

图3 链格孢侵染后甜瓜外果皮CHT变化

Fig.3 Changes in CHT activity in melon epricarp infected by A. alternaria

图4 链格孢侵染后甜瓜内果皮CHT变化

Fig.4 Changes in CHT activity in melon endocarp by A. alternaria

图5 链格孢侵染后甜瓜果肉CHT变化

Fig.5 Changes in CHT activity in melon pulp by A. alternaria

图6 链格孢侵染后甜瓜外果皮GLU变化

Fig.6 Changes in GLU activity in melon epricarp by A. alternaria

图7 链格孢侵染后甜瓜内果皮GLU变化

Fig.7 Changes in GLU activity in melon endocarp by A. alternaria

图8 链格孢侵染后甜瓜果肉GLU变化

Fig.8 Changes in GLU activity in melon pulp by A. alternaria

图9 链格孢侵染后甜瓜外果皮PAL变化

Fig.9 Changes in PAL activity in melon by A. alternaria pericarp

图10 链格孢侵染后甜瓜内果皮PAL变化

Fig.10 Changes in PAL activity in melon by A. alternaria endocarp

图11 链格孢侵染后甜瓜果肉PAL变化

Fig.11 Changes in PAL activity in melon pulp by A. alternaria

图12 链格孢侵染后甜瓜外果皮C4H变化

Fig.12 Changes in C4H activity in melon epricarp A. alternaria

图13 链格孢侵染后甜瓜内果皮C4H变化

Fig.13 Changes in C4H activity in melon endocarp A. alternaria

图14 链格孢侵染后甜瓜果肉C4H变化

Fig.14 Changes in C4H activity in melon pulp A. alternaria

图15 链格孢侵染后甜瓜外果皮4CL变化

Fig.15 Changes in 4CL activity in melon epricarp A. alternaria

图16 链格孢侵染后甜瓜内果皮4CL变化

Fig.16 Changes in 4CL activity in melon endocarp A. alternaria

图17 链格孢侵染后甜瓜果肉4CL变化

Fig.17 Changes in 4CL activity in melon pulp A. alternaria

参考文献 27

[1]	柳涛. 哈密瓜采后病原菌的生长特性及致病机理初步研究[D]. 石河子: 石河子大学, 2014.
	LIU Tao. Preliminary study on the growth characteristics and pathogenic mechanism of postharvest pathogenic bacteria of Hami melon[D]. Shihezi: Shihezi University, 2014.
[2]	Yang B, Yong H G, Chun L W, et al. Melon production in China[J]. Acta Horticulturae, 2007, 731:493-500.
[3]	薛耀碧, 周雅涵, 邓丽莉, 等. 采后果实病程相关蛋白研究进展[J]. 食品工业科技, 2015, 36(6):5.
	XUE Yaobi, ZHOU Yahan, DENG Lili. et al. Research progress of protein related to postharvest fruit course[J]. Science and Technology of Food Industry, 2015, 36(6):5.
[4]	Zhao J, Bao S W, Ma G P, et al. Characterization of Alternaria species associated with muskmelon foliar diseases in Beijing municipality of China[J]. Journal of General Plant Pathology, 2016, 82(1):29-32. DOI URL
[5]	王瑾, 白羽嘉, 冯作山, 等. 链格孢侵染采后甜瓜病程相关蛋白基因表达及酶活性变化规律[J]. 食品科学, 2021, 42(14):94-102.
	WANG Jing, BAI Yujia, FENG Zuoshan, et al. Changes of protein gene expression and enzyme activity in melon infected by Alternaria alternata after harvest[J]. Food Science, 2021, 42(14):94-102. DOI URL
[6]	李梦, 冯作山, 张明明, 等. 厚皮甜瓜果实对链格孢侵染抵抗能力的差异[J]. 食品工业科技, 2018, 39(6):6.
	LI Meng, FENG Zuoshang, ZHANG Mingming, et al. Difference of resistance of muskmelon fruits to Alternaria alternata infection[J]. Science and Technology of Food Industry, 2018, 39(6):6.
[7]	葛永红, 王毅, 毕阳. 柠檬酸处理对厚皮甜瓜黑斑病的抑制及苯丙烷代谢的作用[J]. 食品工业科技, 2013, 34(19):5.
	GE Yonghong, WANG Yi, BI Yang. Effect of citric acid treatment on black spot of muskmelon and phenylpropane metabolism[J]. Science and Technology of Food Industry, 2013, 34(19):5.
[8]	刘瑶瑶, 毕阳, 李国林, 等. 热水和壳聚糖处理对厚皮甜瓜采后病害及苯丙烷代谢的影响[J]. 食品工业科技, 2013, 34(15):5.
	LIU Yaoyao, BI Yang, LI Guolin, et al. Effects of hot water and chitosan treatment on postharvest diseases and phenylpropane metabolism of muskmelon[J]. Science and Technology of Food Industry, 2013, 34(15):5.
[9]	玛尔哈巴·帕尔哈提, 白羽嘉, 王瑾, 等. 采后伽师瓜和86-1甜瓜果实抗链格孢菌侵染研究[J]. 食品与机械, 2018, 34(12):6.
	Maerhaba Paerhati, BAI Yujia, WANG Jin, et al. Study on the resistance of postharvest Jiashigua and 86-1 melon fruits to Alternaria alternata infection[J]. Food and Machinery, 2018, 34(12):6.
[10]	周爱东, 徐小明, 王岚, 等. 植物病原真菌致病基因研究进展[J]. 绿色科技, 2017,(3):113-115.
	ZHOU Aidong, XU Xiaoming, WANG Lan, et al. Research progress of pathogenic genes of plant pathogenic fungi[J]. Green Science and Technology, 2017,(3):113-115.
[11]	刘丹阳, 鲁丁强, 庞广昌. 甜瓜贮藏保鲜技术研究进展[J]. 食品研究与开发, 2021, 42(19):200-205.
	LIU Danyan, LU Dingqiang, PAN Guangchan. Research Progress on Storage Technology of Muskmelon[J]. Food Research and Development, 2021, 42(19):200-205.
[12]	刘利华, 林奇英, 谢华安, 等. 病程相关蛋白与植物抗病性研究[J]. 福建农业学报, 1999, 14(3):6.
	LIU Lihua, LIN Qiying, XIE Huaan, et al. Study on disease-related proteins and plant disease resistance[J]. Journal of Fujian Agriculture, 1999, 14(3):6.
[13]	曾睿慷, 白羽嘉, 王乾乾, 等. 链格孢菌侵染采后伽师瓜抗病关键酶活性变化规律[J]. 食品研究与开发, 2022, 43(16):8-17.
	ZENG Ruikan, BAI Yujia, WANG Qianqian, et al. Changes of disease-resistant key enzyme activities of postharvest Jiashi melon infected by Alternaria alternata[J]. Food Research and Development, 2022, 43(16):8-17.
[14]	张培岭, 黄伟, 马玲, 等. 链格孢侵染对甜瓜病程相关蛋白活性及基因表达的影响[J]. 食品工业科技, 2017, 38(18):6.
	ZHANG Peiling, HUANG Wei, MA Ling, et al. Effect of Alternaria alternata infection on protein activity and gene expression related to melon disease course[J]. Science and Technology of Food Industry, 2017, 38(18):6.
[15]	Wessels J G H, Sietsma J H. Plant carbohydrates[M]// Encyclopedia of plant physiology (New Series).Berlin: SpringerVerlag, 1981.
[16]	Ruiz H J, Sentandreu R. Fungal cell wall synthesis and assembly[J]. Current Topics in Medical Mycology, 1989, 3:168-217. PMID
[17]	张博阳, 朱天辉, 韩珊, 等. 桑氏链霉菌几丁质酶ChiKJ40基因的克隆表达及其抑菌作用[J]. 微生物学通报, 2018, 45(5):11.
	ZHANG Boyang, ZHU Tianhui, HAN Shan, et al. Cloning and expression of chitinase ChiKJ40 gene from Streptomyces sangensis and its antibacterial effect[J]. Bulletin of Microbiology, 2018, 45(5):11.
[18]	温晓丽. 外源磷酸钠对枣果实活性氧和苯丙烷代谢关键酶活性及酚类物质积累的影响[J]. 食品工业科技, 2022, 43(17):381-386.
	WEN Xiaoli. Effects of exogenous sodium phosphate on the activity of key enzymes and phenolic substances in reactive oxygen species and phenylpropane metabolism in jujube fruit[J]. Science and Technology of Food Industry, 2022, 43(17):381-386.
[19]	尚军, 吴旺泽, 马永贵. 植物苯丙烷代谢途径[J]. 中国生物化学与分子生物学报, 2022, 38(11):1467-1476.
	SHANG Jun, WU Wangze, MA Yonggui. Metabolic pathway of plant phenylpropane[J]. Chinese Journal of Biochemistry and Molecular Biol, 2022, 38(11):1467-1476.
[20]	蒋超男, 李灿婴, 李伊涵, 等. 采后核黄素处理对苹果青霉病及活性氧和苯丙烷代谢的影响[J]. 保鲜与加工, 2021, 21(3):1-7.
	JIANG Chaonan, LI Canying, LI Yihan, et al. Effects of postharvest riboflavin treatment on apple penicilliosis and metabolism of active oxygen and phenylpropane[J]. Preservation and Processing, 2021, 21(3):1-7.
[21]	Jiang H, Wang B, Ma L, et al. Benzo(1, 2, 3)thiadiazole 7 carbothioic acids mehyl ester(BTH) promotes tuber wound healing of potato by elevation of pheny lpropanoid metabolism[J]. Postharvest Biology and Technology, 2019, 153:125 -132. DOI:10.1016/j.postharvbio.2019.03.003. DOI
[22]	范存斐, 毕阳, 王云飞, 等. 水杨酸对厚皮甜瓜采后病害及苯丙烷代谢的影响[J]. 中国农业科学, 2012,(3):186-191.
	FAN Cunfei, BI Yang, WANG Yunfei, et al. Effects of salicylic acid on postharvest diseases and phenylpropane metabolism of muskmelon[J]. Scientia Agricultura Sinica, 2012,(3):186-191.
[23]	王瑾, 冯作山, 白羽嘉, 等. 链格孢侵染对伽师瓜果皮活性氧代谢的影响[J]. 现代食品科技, 2019, 35(11):83-92,211.
	WANG Jin, FENG Zuoshan, BAI Yujia, et al. Effects of Alternaria alternata infection on reactive oxygen species metabolism inpericarp of Jiashi muskmelon[J]. Modern Food Science and Technology, 2019, 35(11):83-92,211.
[24]	曹建康, 姜微波, 赵玉梅. 果蔬采后生理生化实验指导[M]. 北京: 中国轻工业出版社, 2007:126-127.
	CAO Jiankang, JIANG Weibo, ZHAO Yumei. Postharvest physiological and biochemical experiment instruction of fruits and vegetables[M]. Beijing: China Light Industry Press, 2007:126-127.
[25]	李梦, 冯作山, 张明明, 等. 甜瓜抵抗链格孢侵染能力差异与活性氧相关代谢的关系[J]. 食品工业科技, 2018, 39(19):7.
	LI Meng, FENG Zuoshan, ZHANG Mingming, et al. The relationship between the difference of the resistance capacity and reactive oxygen species related metabolism in muskmelon inoculated with Alternaria alternata[J]. Science and Technology of Food Industry, 2018, 39(19):7.
[26]	张培岭, 白羽嘉, 马玲, 等. 采后链格孢侵染对甜瓜果实苯丙烷代谢的影响[J]. 保鲜与加工, 2018, 18(6):7.
	ZHANG Peiling, BAI Yujia, MA Ling, et al. Effect of Alternaria alternata infection on phenylpropane metabolism in melon fruit after harvest[J]. Preservation and Processing, 2018, 18(6):7.
[27]	刘筱, 潘静宇, 李永才, 等. 寡雄蛋白(Oligandrin)处理对马铃薯块茎干腐病及苯丙烷代谢的影响[J]. 食品工业科技, 2017, 38(6):7.
	LIU Xiao, PAN Jingyu, LI Yongcai, et al. Effects of Oligandrin treatment on dry rot of potato tuber and phenylpropane metabolism[J]. Science and Technology of Food Industry, 2017, 38(6):7.

甜瓜抗链格孢侵染病程相关蛋白及苯丙烷代谢酶的变化

Changes of related proteins and phenylpropane metabolic enzymes in melon infected by Alternaria alternata

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