环剥对灰枣果实中糖积累及蔗糖代谢相关酶活性的影响

doi:10.6048/j.issn.1001-4330.2019.07.008

新疆农业科学 ›› 2019, Vol. 56 ›› Issue (7): 1244-1252.DOI: 10.6048/j.issn.1001-4330.2019.07.008

• 园艺·林业·生理生化 • 上一篇下一篇

环剥对灰枣果实中糖积累及蔗糖代谢相关酶活性的影响

杨磊, 樊丁宇, 靳娟, 徐叶挺, 周晓明, 冯贝贝, 郝庆

新疆农业科学院园艺作物研究所/农业部新疆地区果树科学观测实验站,乌鲁木齐 830091

收稿日期:2019-03-18 出版日期:2019-07-20 发布日期:2019-09-05
通信作者: 郝庆(1969- )男,新疆阿克苏人,研究员,研究方向为新疆特色果树栽培与技术推广,(E-mail)haoqingxj@sohu.com
作者简介:杨磊(1981-),男,新疆吉木萨尔人,副研究员,硕士,研究方向为果树栽培与生理,(E-mail)yanglei9961@163.com
基金资助:
自治区科技援疆计划(2018E02017);自治区重点研发计划项目(2017B01002-1);新疆农业科学院青年基金项目(xjnkq-2015015)

Related to Sucrose Metabolism in Huizao (Zizyphus jujuba Mill.)

YANG Lei, FAN Ding-yu, JIN Juan, XU Ye-ting, ZHOU Xiao-ming, FENG Bei-bei, HAO Qing

Institute of Horticultural Crops,Xinjiang Academy of Agricultural Sciences / Scientific Observing and Experimental Station of Pomology Xinjiang,Urumqi 830091,China

Received:2019-03-18 Online:2019-07-20 Published:2019-09-05
Correspondence author: HAO Qing (1969- ), Male, Native Place: Akesu, Xinjiang. Research Fellow. Research Field: Cultivation and technology promotion of Xinjiang fruit Trees,(E-mail)haoqingxj@sohu.com
Supported by:
Supported by S & T Assisting Xinjiang Project (2018E02017);Key R & D Program Project of Xinjiang (2017B01002-1); Youth Fund Project of Xinjiang Academy of Agricultural Science (xjnkq-2015015)

摘要/Abstract

摘要： 【目的】研究环剥后枣果实发育过程中糖分含量的变化与蔗糖代谢相关酶活性之间的关系,改善枣果实品质提供理论依据。【方法】以5a生灰枣为对象,测定环剥后果实发育过程中糖分含量的变化和蔗糖代谢相关酶活性。【结果】(1)灰枣环剥和对照的果实在整个发育过程中,果糖、葡萄糖和蔗糖的含量变化基本一致,其积累随着果实的发育总体呈上升趋势。环剥明显提高了灰枣果实果糖、葡萄糖和果实发育后期的蔗糖含量。果实成熟期蔗糖显著高于果糖和葡萄糖的含量,灰枣果实以积累蔗糖为主。(2)环剥有助于提高环剥口愈合前(7月15日)果实内中性转化酶、蔗糖磷酸合成酶、蔗糖合成酶的活性。果实全红期至实成熟,环剥处理果实的蔗糖合成酶合成方向(SSs)活性显著高于对照,是环剥处理果实蔗糖含量高与对照的主要原因。【结论】环剥有利于果实果糖、葡萄糖和蔗糖的积累,SSs活性对环剥处理果实的蔗糖代谢起主要调节作用。

关键词: 灰枣; 环剥; 糖积累; 蔗糖代谢相关酶

Abstract: 【Objective】 To explore the relationship between the changes of sugar content and the activities of enzymes related to sucrose metabolism during the development of jujube fruit after girdling in the hope of providing a theoretical basis for improving the quality of jujube fruits in production. 【Method】We girdled trunk of the 5-year-old jujube trees and measured the content of glucose, fructose, sucrose, starch and the activities of sucrose metabolism-related enzymes and then studied the changes of content and activities of enzymes related to sucrose metabolism. 【Result】The results showed that: (1)The changes in the content of fructose, glucose and sucrose were basically consistent throughout the whole development process in both girdling and control, its accumulation tended to rise with the development of fruit as a whole, but the girdling significantly increased the fructose, glucose and sucrose content in jujube fruit at the later stage of fruit development. 'The content of sucrose was significantly higher than that in fructose and glucose in fruit ripening stage, which indicated that sucrose accumulation was the main content in jujube fruit. (2)Girdlilng was helpful to increase the activities of neutral invertase, sucrose phosphate synthase and sucrose synthase in fruit before the healing of ring peeling (July 15). From the full red period to the real maturity, sucrose synthase activity in the sucrose synthesis direction (SSs) of trunk girdling fruit was significantly higher than that of the control, which was the main reason for the high sucrose content of the fruit in girdling treatment and control. In summary, trunk girding is beneficial to the accumulation of fructose, glucose and sucrose in fruit, and SSs plays a major role in regulating sucrose metabolism in fruit stripped.【Conclusion】 Trunk girdling is beneficial to the accumulation of fructose, glucose and sucrose in the fruit, and SSs plays a major role in regulating the sucrose metabolism of the fruit in girdling treatment.

Key words: huizao(Zizyphus jujuba Mill.); girdling; sugar accumulation; sucrose metabolism related enzymes

中图分类号:

S665

杨磊, 樊丁宇, 靳娟, 徐叶挺, 周晓明, 冯贝贝, 郝庆. 环剥对灰枣果实中糖积累及蔗糖代谢相关酶活性的影响[J]. 新疆农业科学, 2019, 56(7): 1244-1252.

YANG Lei, FAN Ding-yu, JIN Juan, XU Ye-ting, ZHOU Xiao-ming, FENG Bei-bei, HAO Qing. Related to Sucrose Metabolism in Huizao (Zizyphus jujuba Mill.)[J]. Xinjiang Agricultural Sciences, 2019, 56(7): 1244-1252.

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