库尔勒香梨果实可溶性糖积累及代谢相关酶活性变化

doi:10.6048/j.issn.1001-4330.2018.04.009

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (4): 664-673.DOI: 10.6048/j.issn.1001-4330.2018.04.009

库尔勒香梨果实可溶性糖积累及代谢相关酶活性变化

艾沙江·买买提,张校立,梅闯,马凯,闫鹏,王继勋

新疆农业科学院园艺作物研究所/农业部新疆地区果树科学观测试验站,乌鲁木齐 830091

收稿日期:2018-02-24 出版日期:2018-04-20 发布日期:2018-07-18
通信作者: 王继勋(1965-),新疆吉木萨尔人,研究员,硕士,研究方向为果树资源与育种,(E-mail)eewjx@163.com
作者简介:艾山江·买买提(1982-),男,新疆库尔勒人,助理研究员,博士,研究方向为果树生理,(E-mail)aisajan_116@163.com
基金资助:
自治区公益性科研院所基本科研业务经费项目“库尔勒香梨果实发育过程中木质素代谢的变化与石细胞发育的关系研究”(KY2017075)

Soluble Sugar Accumulation and Related Enzyme Activity in the Fruits of Korla Fragrant Pear

Aishajiang Maimaiti, ZHANG Xiao-li, MEI Chuang, MA Kai, YAN Peng,WANG Ji-xun

Research Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences / Scientific Observing and Experimental Station of Pomology (Xinjiang,Ministry of Agriculture, Urumqi 830091,China

Received:2018-02-24 Online:2018-04-20 Published:2018-07-18
Correspondence author: WANG Ji-xun(1965-), male, native place: Jimusaer, Xinjiang, Research Fellow, master, research field: Fruit tree resources and breeding, (E-mail)eewjx@163.com
Supported by:
Basic Scientific R & D Program of Public Welfare Research Institutes of Xinjiang Uygur Autonomous Region of China "Study on the relationship between the changes of lignin metabolism and the development of stone cells during the development of Korla pear fruit".（KY2017075）

摘要/Abstract

摘要： 【目的】研究库尔勒香梨果实发育过程中糖积累特性及相关酶活性的变化规律。【方法】以15年生库尔勒香梨(Pyrus brestschneideri Rehd)为试材,研究其果实发育过程中总糖、淀粉、蔗糖、果糖、葡萄糖和山梨醇含量以及6种糖代谢相关酶活性的变化。【结果】果实幼果期山梨醇为主要糖,占总糖中的比例50%以上,随着果实的发育,山梨醇所占的比例呈下降趋势,到果实成熟期,总糖中的比例降到24.7%。而在中后期果糖、葡萄糖和蔗糖所占的比例缓慢上升,果糖含量超过山梨醇成为果实中的主要糖。成熟果实中可溶性总糖含量为16.16%,果糖、山梨醇、葡萄糖和蔗糖占可溶性总糖中的比例分别为38.7%、24.7%、 22.0%和14.4%。酸性转化酶(AI)和中性转化酶(NI)在果实发育前期活性较弱,随着果实发育而逐渐增高。果实发育早期蔗糖合成酶(SS)活性随果实发育而下降,而后期则随果实发育而逐步增强。蔗糖磷酸合成酶(SPS)活性随果实发育持续上升,在果实发育的中后期急剧升高,与蔗糖迅速积累的时期相一致。山梨醇脱氢酶(SHD)和山梨醇氧化酶(SOX)的活性变化趋势基本相同,随着果实的发育先降低后增高,但前者的变化幅度大,且其活性水平始终显著高于后者。【结论】在果实幼果期山梨醇为主要糖,而成熟期果糖含量超过山梨醇成为果实中的主要糖。果实发育过程中可溶性糖积累主要受山梨醇的影响,受蔗糖、葡萄糖和果糖积累差异的影响较小。

关键词: 库尔勒香梨; 果实发育; 糖积累动态; 糖代谢酶

Abstract: 【Objective】 This project aims to explore the sugar accumulation patterns and enzyme activities in sugar metabolism during fruit development.【Method】15 years old Korla fragrant pear (Pyrus brestschneideri Rehd) trees were used as material to analyze the variation of the major soluble sugar contents and related enzyme activities in sugar metabolism.【Result】Results showed that at early stage, sorbitol was the main component of soluble sugars accounting for more than 50% of total soluble sugars, and the proportion of sobitol tended to decrease along with the development of young fruits. However, the proportion of fructose, glucose and sucrose rose slowly at the later stage, and the fructose content surpassed the sorbitol to become the main sugar in the fruit. In the ripening stage, total soluble sugar contents increased to 16.16%, and fructose, sorbitol, glucose and sucrose contents accounted for 38.7%, 24.7%, 22.0% and 14.4% of total sugar content, respectively. The activities of acid invertase (AI) and neutral invertase (NI) were relatively low at early stage and increased strongly with fruit development. The sucrose synthase activity (SS) reduced at the early stage, and increased gradually at the later period. SPS activity increased slowly during the early stage of fruit growth,but showed a steep rise afterwards when sucrose accumulated sharply at the ripening stage. Variation trends of sorbitol dehydrogenase (SDH) and sorbitol oxidase (SOX) activities were basically the same, decreased at the early stage and then increased at the later stage. 【Conclusion】In the process of fruit development, the accumulation of total soluble sugar is mainly influenced by sorbitol, sucrose, glucose and fructose have little contribution to the total soluble sugar. Sorbitol is the main component of soluble sugars at early stage, However, at the ripening stage, fructose content surpassed the sorbitol to become the main sugar in the fruit.

Key words: Korla fragrant pear;fruit development; sugar accumulation; sugar-metabolizing enzymes

中图分类号:

S661.2

艾沙江·买买提,张校立,梅闯,马凯,闫鹏,王继勋. 库尔勒香梨果实可溶性糖积累及代谢相关酶活性变化[J]. 新疆农业科学, 2018, 55(4): 664-673.

Aishajiang Maimaiti, ZHANG Xiao-li, MEI Chuang, MA Kai, YAN Peng,WANG Ji-xun. Soluble Sugar Accumulation and Related Enzyme Activity in the Fruits of Korla Fragrant Pear[J]. Xinjiang Agricultural Sciences, 2018, 55(4): 664-673.

参考文献

[1] 伍涛,陶书田,张虎平,等. 疏果对梨果实糖积累及叶片光合特性的影响[J]. 园艺学报,2011,38(11):2 041-2 048.
   WU Tao,TAO Shu-tian,ZHANG Hu-ping,et al. (2011). Effects of Fruit Thinning on Fruit Sugar Accumulation and Leaf Photosynthetic Characteristics of Pear [J]. Acta Horticulturae Sinica, 38(11): 2,041-2,048. (in Chinese)
[2] 黄艳花, 曾明. 梨果实糖代谢及调控因子的研究进展[J]. 植物生理学报,2013, 49(8): 709-714.
   HUANG Yan-hua, ZENG Ming. (2013). Research advances in sugar metabolism and regulatory factors in pear fruits plant [J]. Physiology Journal, 49(8): 709-714. (in Chinese)
[3] 霍月青. 砂梨品种资源糖酸及石细胞含量特点研究[D].武汉: 华中农业大学硕士学位论文,2007.
   HUO Yue-qing. (2007). Study on characteristics of sugars,acids and stone cell in different sand pears from germplasm resources [D]. Master Dissertation: Huazhong Agricultural University, Wuhan.(in Chinese)
[4] 姚改芳,张绍铃,曹玉芬,等. 不同栽培种梨果实中可溶性糖组分及含量特征[J]. 中国农业科学,2010,43(20): 4 229-4 237.
   YAO Gai-fang, ZHANG Shao-ling, CAO Yu-fen, et al. (2010). Characteristics of components and contents of soluble sugars in pear fruits from different species [J].Acta Horticulturae Sinica, 43(20): 4,229-4,237. (in Chinese)
[5] 熊碧玲,汪志辉,刘燕. 鲜黄梨果实糖积累及山梨醇转化相关酶活性的变化[J]. 中国南方果树, 2011,40(4): 24-26.
   XIONG Bi-ling,WANG Zhi-hui, LIU Yan. (2011). Changes in sugar accumulation and enzymatic activities of sorbitol metabolism in developing fruit of pyrus pyrifolia cv. Sunhwang [J]. South China Fruits, 40 (4): 24-26. (in Chinese)
[6] 崔艳波,陈慧,乐文全,等. 京白梨与鸭梨正反交后代果实性状遗传倾向研究[J]. 园艺学报,2011,38(2):215-224.
   CUI Yan-bo,CHEN Hui,YUE Wen-quan,et al. (2011). Studies on genetic tendency of fruit characters in reciprocal crosses generation between Jingbaili and Yalipear cultivars [J]. Acta Horticulturae Sinica,38(2):215-224. (in Chinese)
[7] 杨志军,乐文全,张绍铃,等. 鸭梨×京白梨杂交后代果实可溶性糖积累差异以及相关酶活性研究[J]. 园艺学报,2012,39(6):1 055-1 063.
   YANG Zhi-jun,YUE Wen-quan,ZHANG Shao-ling,et al. (2012). The Difference of Soluble Sugar Accumulation and Related Enzymes Activities in Pear Fruit from Hybrid Offspring [J]. Acta Horticulturae Sinica, 39(6):1,055-1,063. (in Chinese)
[8] 李芳芳,何子顺,陶书田,等. 套袋对库尔勒香梨果实发育过程中可溶性糖含量的影响[J]. 果树学报,2014,31(6): 1 072-1 078.
   LI Fang-fang,HE Zi-shun,TAO Shu-tian,et al. (2014). Effects of bagging on soluble sugars contents during fruit development of Korla fragrant pear [J]. Journal of Fruit Science, 31(6): 1,072-1,078. (in Chinese)
[9] 王德孚,杨志军,孙江妹,等,吴俊梨不同品种果实可溶性糖积累差异及代谢相关酶活性[J]. 果树学报,2014,31(1):30-38.
   WANG De -fu,YANG Zhi -jun,SUN Jiang -mei,et al. (2014). Difference in soluble sugar accumulation and related enzyme activity of fruits among different pear cultivars [J]. Journal of Fruit Science, 31(1): 30-38. (in Chinese)
[10] 许让伟. 砂梨果实和叶片中糖积累及代谢相关酶活性变化研究[D]. 武汉: 华中农业大学硕士论文,2009.
   XU Rang-wei. (2009). Studies on changes of sugar accumulatiom and related enzymes activities in sand pear fruits and leaves[D]. Master Dissertation: Huazhong Agricultural University, Wuhan.(in Chinese)
[11] 于年文,李俊才,王家振,等. 南国梨果实糖积累过程中相关酶的作用[J]. 西南农业学报,2011,24(3):919-923.
   YU Nian-wen,LI Jun-cai,WANG Jia-zhen,et al. (2011). Roles of related enzymes in accumulation of sugars in Nanguoli pear fruit[J]. Southwest China Journal, 24(3): 919-923. (in Chinese)
[12] 刘松忠,刘军,朱青青,等. 肥料种类对不同采收期黄金梨糖酸含量和风味的影响[J]. 果树学报,2012,29(5): 804-808.
   LIU Song-zhong,LIU Jun,ZHU Qing-qing,et al. (2012). Effects of manure types on sugar and acid contents and flavor of pear(Pyrus pyrifolia Hawangkumbae)at different mature stages [J]. Journal of Fruit Science, 29(5): 804-808.(in Chinese)
[13] Li, M., Feng, F., & Cheng, L. (2012). Expression patterns of genes involved in sugar metabolism and accumulation during apple fruit development. Plos One, 7(3): e33055.
[14] Ito, A., Sakamoto, D., & Moriguchi, T. (2012). Carbohydrate metabolism and its possible roles in endodormancy transition in japanese pear. Scientia Horticulturae, 144(3): 187-194.
[15] 戴美松,徐飞,施泽彬,等. 砂梨山梨醇转运蛋白(SOT)基因家族成员表达特性及在果实糖积累中的作用初探[J]. 园艺学报,2015,42 (8):1 457-1 466.
   DAI Mei-song,XU Fei,SHI Ze-bin,et al. (2015). Preliminary Study on Expression Characteristics of Sorbitol Transporter(SOT)Gene Family and the Role in Sugar Accumulation in Pyrus pyrifolia Fruits[J].Acta Horticulturae Sinica, 42 (8):1,457-1,466. (in Chinese)
[16] 潘俨,孟新涛,车凤斌,等. 库尔勒香梨果实发育成熟的糖代谢和呼吸代谢响应特征[J]. 中国农业科学,2016, 49(17):3 391-3 412.
   PAN Yan, MENG Xin-tao, CHE Feng-bin, et al. (2016).Metabolic Profiles of Sugar Metabolism and Respiratory Metabolism of Korla Pear (Pyrus sinkiangensis Yu) Throughout Fruit Development and Ripening[J]. Scientia Agricultura Sinica, 49(17):3,391-3,412.(in Chinese)
[17] 袁晖,韦云,李馨玥,等. '南果梨'及其芽变'南红梨'果实中糖分积累与相关基因表达差异分析[J]. 果树学报, 2017,34(5):534-540.
   YUAN Hui, WEI Yun, LI Xin-yue, et al. (2017). Differences in sugar accumulation and the related gene expression infruit development between'Nanguo'and its mutant'Nanhong'pears[J].Journal of Fruit Science, 34(5): 534-540. (in Chinese)
[18] 赵世杰. 植物生理学实验指导[M].北京:中国农业科技出版社,1998.
   ZHAO Shi-jie (1998). Experimental guidance of plant physiology [M]. Beijing: China Agricultural Science and Technology Press. (in Chinese)
[19] Yamaguchi, H. (1996). Changes in the amounts of the nad-dependent sorbitol dehydrogenase and its involvement in the development of apple fruit. American Society for Horticultural Science, 121(5): 848-852.
[20] 王永章, 张大鹏. '红富士'苹果果实蔗糖代谢与酸性转化酶和蔗糖合酶关系的研究[J]. 园艺学报, 2001,28(3): 259-261.
   WANG Yong-zhang, ZHANG Da-peng. (2001). Study on the relationships between acid invertase, sucrose synthase and sucrose metabolism in red Fuji apple fruit[J]. Acta Horticulturae Sinica, 28(3): 259-261. (in Chinese)
[21] 魏建梅. 苹果(Malus domestica Borkh.) 果实质地品质发育及采后调控的生理和分子基础[D]. 杨凌: 西北农林科技大学博士论文,2009.
   WEI Jian-mei. (2009). Study on the physiological and melecular mechanism of fruit texture development and post-harvest regulation of apple (Malus domestica Borkh.) [D]. PhD Dissertation. Northwest A&F University, Yangling. (in Chinese)
[22] 柯凡君, 张虎平, 陶书田, 等. 套袋对梨果实发育过程中糖组分及其相关酶活性的影响[J]. 西北植物学报, 2011,31(7):1 422-1 427.
   KE Fan-jun, ZHANG Hu-ping, TAO Shu-tian , et al. (2011). Sugar Component Contents and Metabol ism-related Enzyme Activities in Developing Pear Fruits after Bagging[J].Acta Bot .Boreal .-Occident .Sin. ,31 (7):1,422-1,427. (in Chinese)
[23] Rolland, F., & Sheen, J. (2002). Sugar sensing and signaling in plants. Plant Cell, 14(Suppl): s185.
[24] 李馨玥,李通,袁晖,等. 南果梨果实发育过程中糖分积累与相关基因表达分析[J]. 果树学报,2016,33(增刊):59-64.
   LI Xin-yue,LI Tong,YUAN Hui,et al. (2016). Sugar accumulation and expression of related genes in'Nanguo'pear fruit[J].Journal of Fruit Science, 33(supplement):59-64. (in Chinese)
[25] 梁东. 苹果山梨醇代谢相关基因的分子特性研究[D]. 杨凌: 西北农林科技大学博士论文,2010.
   LIANG Dong (2010). Molecular characteristics of genes involved in sorbitol metabolism in apple [D] PhD Dissertation. Northwest A&F Universit, Yangling. (in Chinese)
[26] Gao, Z., Maurousset, L., Lemoine, R., Yoo, S. D., Van, N. S., & Loescher, W. (2003). Cloning, expression, and characterization of sorbitol transporters from developing sour cherry fruit and leaf sink tissues. Plant Physiology, 131(4): 1,566-1,575.
[27] Zhang, H. P., Wu, J. Y., Tao, S. T., Wu, T., Qi, K. J., & Zhang, S. J., et al. (2014). Evidence for apoplasmic phloem unloading in pear fruit. Plant Molecular Biology Reporter, 32(4):931-939.
[28] 常尚连, 于贤昌, 于喜艳. 西瓜果实发育过程中糖分积累与相关酶活性的变化[J]. 西北农业学报,2006,15(3): 138-141.
   CHANG Shang-lian, YU Xian-chang, YU Xi-yan. (2006). Change of sugar accumulation and related enzyme activity during fruit development of watermelon [J]. Acta Agriculturae Boreali-occidentalis Sinica, 15(3): 138-141. (in Chinese)
[29] 陈露露,王涛,黄雪燕,等. 大棚翠冠梨果实糖积累及蔗糖代谢相关酶活性的研究[J]. 果树学报, 2011,28(3): 400-405.
   CHEN Lu -lu,WANG Tao,HUANG Xue -yan,et al. (2011). Study on enzyme activities involved in sugar accumulation and sucrose metabolization in Cuiguan pear under plastic tunnel culture [J]. Journal of Fruit Science, 28(3): 400-405. (in Chinese)
[30] 贾海锋. 蔗糖及茉莉酸信号在草莓果实发育中的作用及其机理分析[D]. 北京: 中国农业大学博士论文,2013.
   JIA Hai-feng. (2013). Roles and mechanmisms of sucrose and jasmonic acid signals in the regulation of strawberry fruit development[D]. PhD Dissertation. China Agricultural University, Beijing. (in Chinese)
[31] 陈俊伟, 张上隆, 张良诚. 果实中糖的运输、代谢与积累及其调控[J]. 植物生理与分子生物学学报, 2004,30(1): 1-10.
   CHEN Jun-wei, ZHANG Shang-long, ZHANG Liang-cheng. (2004). Sugar transport, metabolism, accumulation and their regulation in fruits [J]. Journal of Plant Physiology and Molecular Biology, 30(1): 1-10. (in Chinese)
[32] Schultz J A, Juvik J A. (2004). Current models for starch synthesis and the sugary enhancer1 (se1) mutation in Zea mays[J]. Plant Physiol Biochem, 42(6):457-464.
[33] 张红. 风味甜瓜果实酸味形成机理及糖酸遗传和积累模拟研究[D]. 杨凌: 西北农林科技大学博士论文,2009.
   ZHANG Hong.(2009). Mechanism of organic acid formation and genetics of sugar and acid and simulation of Accumulation in flavor melon fruit [D]. PhD Dissertation. Northwest A&F University, Yangling. (in Chinese)
[34] 郝敬虹, 李天来, 孟思达, 等, 孙利萍夜间低温对薄皮甜瓜果实糖积累及代谢相关酶活性的影响[J]. 中国农业科学, 2009,42(10): 3 592-3 599.
   HAO Jing-hong, LI Tian-lai, MENG Si-da, et al. (2009). Effects of night low temperature on sugar accumulation and sugar-metabolizing enzyme activities in melon fruit [J]. Scientia Agticultura Sinica, 42(10): 3,592-3,599. (in Chinese)

库尔勒香梨果实可溶性糖积累及代谢相关酶活性变化

Soluble Sugar Accumulation and Related Enzyme Activity in the Fruits of Korla Fragrant Pear

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[13]	章世奎, 阿布来克·尼牙孜, 王绍鹏, 王亚铜, 孙召展, 樊国全. 烯效唑对库尔勒香梨枝叶生长及果实品质的影响[J]. 新疆农业科学, 2020, 57(9): 1674-1680.
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