干旱胁迫下油菜素内酯浸种对棉花种子萌发期NAC转录因子表达的影响

doi:10.6048/j.issn.1001-4330.2017.08.005

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (8): 1414-1421.DOI: 10.6048/j.issn.1001-4330.2017.08.005

干旱胁迫下油菜素内酯浸种对棉花种子萌发期NAC转录因子表达的影响

严晓红, 包秋娟, 张富春

新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐 830046

收稿日期:2017-05-10 发布日期:2020-07-22
通信作者: 张富春(1962-),男,教授,博士,研究方向为分子生物学,(E-mail)zfcxju@xju.edu.cn
作者简介:严晓红(1992-),女,硕士研究生,研究方向为植物分子生物学,(E-mail)527863765 @qq.com
基金资助:
国家自然科学基金-新疆联合基金重点项目“棉花水分高效利用的关键基因的挖掘及抗旱种质材料的创制”(U1303282)

Influences of Brassinolide under Drought Stress on the Expressions of NAC Transcription Factors in Germination Stage of Cotton Seeds

YAN Xiao-hong, BAO Qiu-juan, ZHANG Fu-chun

Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China

Received:2017-05-10 Published:2020-07-22
Correspondence author: ZHANG Fu-chun(1962-),male,native place: Urumqi,Xinjiang. Professor, research field:Molecular biology. (E-mail)zfcxju@xju.edu.cn
Supported by:
Joint key fund of National Natural Science Foundation of China and Xinjiang "Exploring key genes related to high-efficiency water utilizationand creating of drought resistant materials in cotton"(U1303282)

摘要/Abstract

摘要： 【目的】研究干旱胁迫下油菜素内酯浸种棉花种子萌发期NAC转录因子的表达变化,分析NAC转录因子在油菜素内酯(brassinolide,BR)调控棉花抗旱中的作用。【方法】根据陆地棉转录因子NAC家族的基因序列,扩增获得棉花新陆早17号NAC家族中的6个NAC基因(GhNAC1-6)。实时荧光定量PCR检测干旱胁迫下不同油菜素内酯浸种后,棉花种子萌发期子叶和胚根NAC基因的表达变化。【结果】棉花子叶中的GhNAC1在干旱胁迫下受不同浓度油菜素内酯的抑制,GhNAC2和GhNAC3受0.25 mg/L油菜素内酯诱导表达,GhNAC5只受0.5 mg/L油菜素内酯诱导表达,而GhNAC4和GhNAC6表达无显著变化。在棉花胚根中,GhNAC1同样受不同浓度油菜素内酯的抑制,GhNAC2和GhNAC3受0.25 mg/L油菜素内酯诱导,同时GhNAC3在2 mg/L油菜素内酯浸种也呈现高表达,GhNAC5无显著变化,GhNAC4和GhNAC6则受高浓度油菜素内酯诱导表达。【结论】棉花种子萌发期在干旱胁迫下,棉花种子萌发期子叶和胚根的GhNAC1的表达受油菜素内酯抑制,GhNAC2和GhNAC3受低浓度油菜素内酯的表达诱导,油菜素内酯能够影响GhNAC的表达响应干旱胁迫。

关键词: 棉花; 种子萌发; 干旱胁迫; 油菜素内酯; NAC转录因子; 基因表达

Abstract: 【Objective】 In order to investigate the expression of NAC transcription factors during seed germination of cotton(Gossypium hirsutum)under drought stress and to analyze the function of NAC transcription factors in the pathway of brassinolide (BR) regulation for drought resistance in cotton. 【Method】The gene sequences of upland cotton NAC transcription factor family were used to amplify the 6 genes of GhNAC from cotton Xinluzao17. Quantitative RT-PCR was used to detect the change of NAC gene expression in cotyledon and radicle of cotton seeds at germination stage after seed soaked with different concentrations of brassinolide under drought stress. 【Result】In cotton cotyledons,the expressions of GhNAC1under drought stress were suppressed by different concentrations of brassinolide, the expressions of GhNAC2 and GhNAC3 were induced by 0.25 mg/L brassinolide. The expressions of GhNAC5 were only induced by 0.5 mg/L brassinolide, and the expressions of GhNAC4 and GhNAC6 did not change significantly. However, in cotton radicle, the expressions of GhNAC1 were also inhibited by different concentrations of brassinolide, the expressions of GhNAC2 and GhNAC3 were induced by 0.25 mg/L brassinolide. At same time, high expressions of GhNAC3 were induced by 2 mg/L brassinolide, the expressions of GhNAC5 did not change significantly, the expressions of GhNAC4 and GhNAC6 were induced by the high concentration of brassinosteroids. 【Conclusion】The expressions of GhNAC1 in cotton cotyledons and radicles during seed germination were inhibited by brassinolide, and the expressions of GhNAC2 and GhNAC3 was induced by low concentration of brassinolide. The results showed that brassinolide could affect the expression of GhNAC in response to drought stress.

Key words: short ultivars; growth stages; major gene plus polygene inheritance; genetic analysis

中图分类号:

S565.4

严晓红, 包秋娟, 张富春. 干旱胁迫下油菜素内酯浸种对棉花种子萌发期NAC转录因子表达的影响[J]. 新疆农业科学, 2017, 54(8): 1414-1421.

YAN Xiao-hong, BAO Qiu-juan, ZHANG Fu-chun. Influences of Brassinolide under Drought Stress on the Expressions of NAC Transcription Factors in Germination Stage of Cotton Seeds[J]. Xinjiang Agricultural Sciences, 2017, 54(8): 1414-1421.

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干旱胁迫下油菜素内酯浸种对棉花种子萌发期NAC转录因子表达的影响

Influences of Brassinolide under Drought Stress on the Expressions of NAC Transcription Factors in Germination Stage of Cotton Seeds

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