Studies on Tissue Specific Regulation of OsHOX6 Promoter in Rice

doi:10.6048/j.issn.1001-4330.2019.10.005

Xinjiang Agricultural Sciences ›› 2019, Vol. 56 ›› Issue (10): 1800-1805.DOI: 10.6048/j.issn.1001-4330.2019.10.005

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Studies on Tissue Specific Regulation of OsHOX6 Promoter in Rice

HE Dan¹, Yushanjiang Maimaiti², YANG Du², HAN Shen², Slamet-Loedin I.H.³

1.Forestry Technology Extension General Station of Xinjiang Uygur Autonomous Region, Urumqi 830000, China;
2.Key Laboratory of Intergraded Management of Harmful Crop Vermin in China North-Western Oasis, Ministry of Agriculture, P. R. China/Research Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
3.Gene Transformation Lab, Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Banos, Laguna, Philippines

Received:2019-07-12 Published:2019-11-22
Correspondence author: Yushanjiang Maimaiti(1978-) ,Male,Born in Xinjiang ,Doctor, Associate researcher, The research direction is study on the mechanism of disease resistance and stress resistance of crops,(E-mail) yusanjan418@163.com

水稻OsHOX6启动子的组织特异性表达

何丹¹, 玉山江·麦麦提², 杨渡², 韩盛², Inez Hortense Slamet-Loedin³

1.新疆维吾尔自治区林业技术推广总站,乌鲁木齐 830000;
2.农业部西北荒漠绿洲作物有害生物综合治理重点实验室新疆农业科学院植物保护研究所,乌鲁木齐 830091;
3.国际水稻研究所作物育种和生物化学系转基因研究室, 菲律宾

通讯作者: 玉山江·麦麦提(1978-),男,新疆人,副研究员,博士,研究方向为农作物抗病抗逆机理,(E-mail)yusanjan418@163.com
作者简介:何丹(1989-),女,四川人,助理工程师,硕士,研究方向为植物生理,(E-mail)10780365098@qq.com
基金资助:
自治区公益性科研院所基本科研业务费专项(KY2014036)

Abstract

Abstract: 【Objective】 To study the expression of OsHOX6 gene promoter, a member of homeodomain-leucine zipper (HD-Zip I) transcription factor family in rice (Oryza sativa L.) 【Method】 The plant expression vector with fusion gene, OsHOX6 promoter and GUS gene was established, and IR64 was transformed by the agro bacterium-mediated transformation method, then roots, stems, leaves and pollen and other organs in transformed plants during in the different growing stage were dyed with X-Gluc. 【Result】 The transgenic plants with hygromysine resistance and positive detection by PCR were obtained. It showed that some blue spots in the lateral roots, pollen and wound tissue in the plants with fusion gene (OsHOX6 promoter and GUS gene) were detected by using X-Gluc dye, while other organs did not display blue spots, It was found that the green spots were concentrated in the root cortex, indicating that the rice OsHOX6 gene promoter could drive the GUS gene and express it specifically on the transgenic rice lateral roots and pollen. 【Conclusion】 The promoter is specifically expressed in rice lateral roots and pollen, which will be further studied in the study of genes related to lateral roots and endocortical formation in rice.

Key words: rice; gene OsHOX6; specific expression; lateral roots; transgenic

摘要： 【目的】研究水稻HD-ZipⅠ转录因子家族的成员OsHOX6基因启动子的表达。【方法】通过构建水稻OsHOX6基因启动子与GUS基因融合表达载体,利用农杆菌(Agrobacterium)介导,以未成熟水稻胚作为试验材料,转化到水稻IR64,通过PCR检测和潮霉素抗性筛选出阳性的转基因植株,从不同组织取样品,进行X-Gluc染色并观察。【结果】转基因植株的叶、根、茎、花等器官经过X-Gluc染色后,主要在侧根、花粉以及组织损伤部分出现蓝色斑点,其它组织均未检测出蓝色斑点,观察根解剖结构,绿色斑点集中在根内皮层。【结论】 水稻OsHOX6基因启动子能够驱动GUS基因,在转基因水稻侧根和花粉上特异表达。

关键词: 水稻, OsHOX6基因, 特异性表达, 侧根和花粉, 转基因

CLC Number:

:S511

HE Dan, Yushanjiang Maimaiti, YANG Du, HAN Shen, Slamet-Loedin I.H.. Studies on Tissue Specific Regulation of OsHOX6 Promoter in Rice[J]. Xinjiang Agricultural Sciences, 2019, 56(10): 1800-1805.

何丹, 玉山江·麦麦提, 杨渡, 韩盛, Inez Hortense Slamet-Loedin. 水稻OsHOX6启动子的组织特异性表达[J]. 新疆农业科学, 2019, 56(10): 1800-1805.

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Studies on Tissue Specific Regulation of OsHOX6 Promoter in Rice

水稻OsHOX6启动子的组织特异性表达

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