Identification of S-genotypes of Native Pear Cultivars in Xinjiang

doi:10.6048/j.issn.1001-4330.2018.02.006

Abstract

Abstract: 【Objective】 To provide theoretical basis for the selection of pollinated varieties and genetic improvement of Korla pear by classifying and studying the S genotypes of 9 native pear varieties in Xinjiang.【Method】A specific primer was designed to amplify the genomic DNA of 9 native pear varieties in Xinjiang, including PCR amplification, fragment recovery, cloning, sequencing, and the analysis of each sequence by bioinformatics software.【Result】The S-genotypes of the seven cultivars were identified as follows: Hesejujuli S28S4, Luntaijujuli S28S4, Hesejujuli:S28S4,Luntaijujuli:S28S4,Huochengdonghuangli:S28S1,Bayueli:S28S4,Kuerlehuangsuanli:S22S35,Jujuli:S28S4,Kuikeamute1hao:S36S34S22.One gene was identified as S8Sx and another gene as S28Sx in Keteamute Pear and Qipanli Pear, respectively.【Conclusion】It has enriched the s-genotypes information of pear cultivars in China and provided a certain guidance gist for high yield cultivation and genetic improvement of Korla pear.

Key words: native pear cultivars in Xinjiang; S-genotype; DNA sequence analyses

摘要： 【目的】鉴定与研究9个新疆地方梨品种S基因型,为库尔勒香梨授粉品种的筛选及其遗传改良提供理论依据。【方法】设计特异性引物对9 个新疆地方梨品种的基因组DNA 进行PCR扩增,片段回收、克隆、测序,使用生物信息学软件对各序列进行分析。【结果】褐色句句梨、轮台句句梨、霍城冬黄梨、八月梨、库尔勒黄酸梨、句句梨、奎克阿木特1号等7个品种的S基因型分别是:S28S4、S28S4、S28S1、S28S4、S22S35、S28S4、S36S34S22;可特阿木特和棋盘梨各鉴定出一条基因,S基因型分别是 S8Sx和 S28Sx。【结论】确定了7个新疆地方梨品种S基因型,可特阿木特和棋盘梨中各有 1条 S 基因没有被鉴别出。丰富了我国梨资源的S基因型信息,为库尔勒香梨的丰产栽培和遗传改良提供了依据。

关键词: 新疆地方梨品种, S基因型, DNA 序列分析

CLC Number:

S188
S661.2

ZHANG Xiao-li, XU Ye-ting, Aishajiang Maimaiti, XU Juan, DENG Li, WANG Ji-xun. Identification of S-genotypes of Native Pear Cultivars in Xinjiang[J]. Xinjiang Agricultural Sciences, 2018, 55(2): 246-252.

张校立,徐叶挺,艾沙江·买买提,许娟,邓莉,王继勋. 新疆地方梨品种S基因型的鉴定[J]. 新疆农业科学, 2018, 55(2): 246-252.

References 21

[1]	新疆维吾尔自治区统计局.新疆统计年鉴[J].北京:中国统计出版社,2017:383.Statistics Bureau of Xinjiang Uyghur Autonomous Region (2017). Xinjiang Statistical Yearbook [J]. Beijing: China Statistics Press:383.(in Chinese).
[2]	吕文娟,冯建荣,刘小芳,等. '库尔勒香梨'自交不亲和S-RNase等位基因全长的克隆与分析[J]. 分子植物育种,2017,15(5):1 639-1 647.L Wen-juan, FENG Jian-rong, LIU Xiao-fang et al. (2017). Cloning and Analysis of Self-incompatibility S-RNase allelic Genes in Korla Fragrant Pear [J]. Molecular Plant Breeding, 15(5):1,639-1,647. (in Chinese)
[3]	徐胜利,陈小青,徐崇志,等.新疆梨系统品种花粉直感对香梨果萼发育及品质的影响[J].山西果树,2014,(5):3-6. XU Sheng-li, CHEN Xiao-qing, XU Chong-zhi et al. (2014). The xenia ofP. sinkiangensis Yu cultivars effects on Fruit calyx growth and quality [J]. Shanxi Fruits, (5):3-6. (in Chinese)
[4]	Kikuchi A (1929). Investigations in 1927 and 1928. 1. Paterclinical incompatibility in the Japanese pear Okistse Hort Soc, 24:1-6. (in Japanese).
[5]	Machida, Y. (1982). S genotype of several cultivars of japanese pear and doubts about the parents of 'Housui'. Abstr.japan.soc.hort.sci.autumn Meet. (in Japanese)
[6]	Terami, H. (1946). Analysis of the sterility factors existing in varieties of the japanese pear (pyrus serotina rehd. var. culta rehd.). Studies Hort.inst.kyoto Imp.univ, (3):267-271. (in Japanese)
[7]	Castillo, C., Takasaki, T., Saito, T., Norioka, S., & Nakanishi, T. (2002). Cloning of the S8-rnase (S8-allele) of Japanese pear (pyrus pyrifolia nakai). Plant Biotechnology, 19(1): 1-6.
[8]	Kim, H. T., Hirata, Y., Kim, H. J., & Nou, I. S. (2006). The presence of a new s-rnase allele (s 10 ) in asian pear ( pyrus pyrifolia, (burm; nakai)). Genetic Resources & Crop Evolution, 53(7): 13,755-13,83.
[9]	Zisovich, A., Stern, R., Shafir, S., & Goldway, M. (2004). Identification of seven s-alleles from the european pear (pyrus communis) and the determination of compatibility among cultivars. Journal of Pomology & Horticultural Science, 79(1): 101-106.
[10]	Takasaki, T., Moriya, Y., Okada, K., Yamamoto, K., Iwanami, H., & Bessho, H., et al. (2006). Cdna cloning of nine s alleles and establishment of a pcr-rflp system for genotyping European pear cultivars. Theoretical & Applied Genetics, 112(8): 1,543-1,552.
[11]	Mota, M., Tavares, L., & Oliveira, C. M. (2007). Identification of s -alleles in pear ( pyrus communis, l.) cv. 'rocha' and other european cultivars. Scientia Horticulturae, 113(1): 13-19.
[12]	Sanzol, J. (2009). Pistil-function breakdown in a new -allele of european pear, confers self-compatibility. Plant Cell Reports, 28(3): 457-467.
[13]	Gharehaghaji, A. N., Arzani, K., Abdollahi, H., Shojaeiyan, A., Dondini, L., & Franceschi, P. D. (2014). Genomic characterization of self-incompatibility ribonucleases in the Central Asian pear germplasm and introgression of new alleles from other species of the genus pyrus. Tree Genetics & Genomes, 10(2): 411-428.
[14]	乌云塔娜.中国白梨自交不亲和基因的分离鉴定[D]. 长沙: 中南林学院博士论文,2003.Wuyuntana. (2003). Isolation and identification of self-incompatible genes of Chinese pyruse bretschneideri [D].PhD Dissertation. The Central-South Forestry University, Changsha.
[15]	Tan, X. F., Zhang, L., Wuyun, T. N., Yuan, D. Y., Cao, Y. F., & Jiang, A. F., et al. (2007). Molecular identification of two new self-incompatible alleles (s-alleles) in chinese pear (pyrus bretschneideri). Journal of Plant Physiology & Molecular Biology, 33(1): 61-70.
[16]	陈慧,张树军,张绍铃,等.40个梨品种S基因型的鉴定及S基因频率分析[J].南京农业大学学报, 2013,36(5):21-26.CHEN Hui, ZHANG Shu-jun, ZHANG Shao-ling, et al. (2013). Identification of S-genotypes in forty pear cultivars and analysis of S-RNase genes frequency in Pyrus [J]. Journal of Nanjing Agricultural University, 36(5):21-26. (in Chinese)
[17]	江南, 张琳, 谭晓风,等. 基于 cDNA 芯片的梨品种 S 基因型鉴定及新 S-RNase 基因进化分析[J].植物遗传资源学报, 2017,18(3): 520-529.JIANG Nan, ZHANG Lin, TAN Xiao-feng, et al. (2017). Detection of Pear S-genotypes and Evolutionary Analysis of Novel S-RNase Genes Identified by cDNA Microarray-based Method [J]. Journal of Plant Genetic Resources, 18(3): 520-529. (in Chinese)
[18]	沈德绪.果树育种学[M].北京:中国农业出版社,1995:175.SHENG De-su.(1995). Fruit tree thremmatology [M]. Beijing: China Agriculture Press: 175. (in Chinese)
[19]	滕元文,柴明良,李秀根.梨属植物分类的历史回顾及新进展[J].果树学报,2004,(3):252-257.TENG Yuan-wen, CHAI Ming-liang, LI Xiu-gen. (2004). A Historic Retrospect and the Progress in the Taxonomy of the Genus pyrus.[J]. Journal of Fruit Science, (3):252-257. (in Chinese)
[20]	Teng, Y. Y., Tanabe, K., Tamura, F., & Itai, A. (2001). Genetic relationships of pear cultivars in xinjiang, china, as measured by rapd markers. Journal of Horticultural Science & Biotechnology, 76(76):771-779.
[21]	马兵钢,牛建新,吴忠华,等.新疆主要梨品种亲缘关系的分子标记分析[J].石河子大学学报(自然科学版),2004,(2):97-102.MA Bing-gang, NIU Jian-xin, WU Zhong-hua, et al. (2004). Molecular Markers Analysis of Main Cultivars Relationship between Pyrus in Xinjiang [J]. Journal of Shihezi University (Natural Science) , (2):97-102. (in Chinese)