Metabolomics analysis of Bacillus velezensis JTB8-2 induced tomato antagonism towards Orobanche aegyptiaca based on defense enzyme

doi:10.6048/j.issn.1001-4330.2024.10.007

Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (10): 2396-2407.DOI: 10.6048/j.issn.1001-4330.2024.10.007

• Crop Genetics and Breeding · Germplasm Resources · Molecular Genetics · Cultivation Physiology · Physiology and Biochemistry • Previous Articles Next Articles

Metabolomics analysis of Bacillus velezensis JTB8-2 induced tomato antagonism towards Orobanche aegyptiaca based on defense enzyme

ZHU Xiafen¹^,²(), HE Wei², LUO Wenfang², ZHOU Junhui², LI Kemei¹(), XU Jianjun²()

1. College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China
2. Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis Ministry of Agriculture / Xinjiang Key Laboratory of Agricultural Biosafety / Institute of Plant Protection/Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2024-04-10 Online:2024-10-20 Published:2024-11-07
Correspondence author: LI Kemei, XU Jianjun
Supported by:
Project of Natural Science Foundation of Xinjiang Uygur Autonomous Region(2022D01A91);Project for Supporting Stable Agricultural Sci-Tech Renovation(xjnkywdzc-2022004);Earmarked Fund for XJARS(XJARS-07)

基于防御酶与代谢组学分析贝莱斯芽孢杆菌JTB8-2诱导番茄拮抗瓜列当机制

朱夏芬¹^,²(), 何伟², 罗文芳², 周军辉², 李克梅¹(), 许建军²()

1.新疆农业大学农学院,乌鲁木齐 830052
2.新疆农业科学院植物保护研究所/农业部西北荒漠作物有害生物综合治理重点实验室/新疆农业生物安全重点实验室,乌鲁木齐 830091

通讯作者: 李克梅,许建军
作者简介:朱夏芬(1999-),女,山西晋城人,硕士研究生,研究方向为列当生物防治,(E-mail)1845724579@qq.com
基金资助:
新疆维吾尔自治区自然科学基金项目(2022D01A91);农业科技创新稳定支持项目(xjnkywdzc-2022004);新疆现代农业产业技术体系(XJARS-07)

Abstract

Abstract:

【Objective】 Orobanche aegyptiaca is a completely parasitic, malignant weed. Bacillus velezensis JTB8-2 demonstrates potent inhibitory capabilities against O. aegyptiaca. Investigating its mechanism, so research into it might provide a solid theoretical foundation for the application of this strain in the field. 【Methods】 In order to investigate the molecular mechanism of B. elezensis JTB8-2-induced resistance to O. aegyptiaca in tomato, we employed pot culture, enzyme activity assays, and ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MRM-MS/MS). 【Results】 The findings revealed that the application of B. elezensis JTB8-2 fermentation broth to tomatoes at 20, 30, and 40 days after transplantation resulted in a parasitic O. aegyptiaca tubercle count of 0.33, 0.17, and 0.33, respectively. This represented a reduction of 81.67%, 96.96%, and 95.54% compared to the control group, and a reduction of 50.75%, 79.52%, and 50.75%, respectively, compared to the NB medium treatment. The catalase (CAT) activity of tomato roots was found to be higher than that of both the medium treatment and water control group after 3, 20, and 30 days. Moreover, differences were observed in the content of 11 metabolites among 28 plant hormone metabolites in tomato roots. The levels of cytokinins and auxin compounds in tomato roots treated with strain JTB8-2 were higher than those in the water control group. 【Conclusion】 The upregulation of catalase activity, cytokinin, and auxin-related hormones in tomato roots treated with strain JTB8-2 may elicit a systemic resistance response in the plant, thereby reducing the severity of O. aegyptiaca parasitism.

Key words: Orobanche aegyptiaca; Bacillus velezensis; defensive enzyme; metabolomics; induced resistance

摘要：

【目的】 瓜列当是全寄生恶性杂草,贝莱斯芽孢杆菌JTB8-2能够有效防除瓜列当,探明其作用机制可以为该菌株的田间应用提供理论依据。【方法】 采用盆栽、酶活测定及超高效液相色谱-质谱联合(UHPLC-MRM-MS /MS)技术,分析贝莱斯芽孢杆菌JTB8-2诱导番茄抗瓜列当机制。【结果】 贝莱斯芽孢杆菌JTB8-2发酵液处理在番茄移栽后20、30和40 d时,寄生的瓜列当结节数量分别为0.33、0.17和0.33个/株,较NB培养基处理分别减少了50.75%、79.52%和50.75%,较清水对照分别减少了81.67%、96.96%和95.54%。菌株发酵液浇灌番茄植株3、20和30 d后,番茄根部过氧化氢酶(CAT)活性均高于NB培养基处理和清水对照;番茄根系中28种植物激素类代谢物中 11种代谢物含量具有差异,菌株JTB8-2处理的番茄根系中细胞分裂素类与生长素类化合物含量高于清水对照。【结论】 菌株JTB8-2处理的番茄根系中过氧化氢酶活性、细胞分裂素与生长素相关激素的增加诱导了番茄系统抗性,减少了瓜列当寄生量。

关键词: 瓜列当, 贝莱斯芽孢杆菌, 防御酶, 代谢组学, 诱导抗性

CLC Number:

S641.2
S188

ZHU Xiafen, HE Wei, LUO Wenfang, ZHOU Junhui, LI Kemei, XU Jianjun. Metabolomics analysis of Bacillus velezensis JTB8-2 induced tomato antagonism towards Orobanche aegyptiaca based on defense enzyme[J]. Xinjiang Agricultural Sciences, 2024, 61(10): 2396-2407.

朱夏芬, 何伟, 罗文芳, 周军辉, 李克梅, 许建军. 基于防御酶与代谢组学分析贝莱斯芽孢杆菌JTB8-2诱导番茄拮抗瓜列当机制[J]. 新疆农业科学, 2024, 61(10): 2396-2407.

Figures/Tables 10

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时间 Time (d)	处理 Treatments	1-氨基环丙烷羧酸 1-Aminocyclo propanecar boxylic acid	吲哚-3-乙酸 Indole-3- acetic acid	顺式-玉米素 Cis-Zeatin	异戊烯基腺苷 Isopentenyl adenosine	反式玉米素核苷 trans- Zeatin- riboside	褪黑素 Melatonine	水杨酸 Salicylic acid	3-吲哚甲醛 Indole- 3-carboxa ldehyde	茉莉酸 Jasmonic acid	二氢茉莉酸 Dihydrojas monic acid	脱落酸 Abscisic acid
3	CK	901.32±47.38^b	179.69±6.73^b	5.02±1.02 ^b	2.9±0.23^a	201.81±24.05^b	0.74±0.01 ^a	603.92±63.52^b	18.40±0.83 ^b	331.88±124.89 ^b	8.52±0.97 ^b	338.90±20.61^b
	NB	1 727.7±139.06^a	172.96±4.68^b	11.51±0.62 ^a	4.46±1.15 ^a	344.26±55.36^a	0.76±0.03 ^a	1 135.76±102.83 ^a	36.71±0.85 ^b	608.77±44.14^a	22.05±3.56 ^a	488.11±40.29^a
	JTB8-2	1 668.43±122.45 ^a	212.71±3.42^a	13.23±1.06 ^a	2.97±0.21 ^a	183.79±8.29^b	0.84±0.08 ^a	642.91±48.71^b	73.36±10.18^a	205.02±13.87^b	13.71±0.68 ^b	417.70±21.58^ab
20	CK	867.28±27.13^b	165.87±2.79 ^a	0	3.37±0.41 ^a	155.52±38.42^b	0.81±0.02 ^a	1 077.13±150.23 ^a	15.32±1.90 ^a	211.33±39.76 ^b	15.20±2.64 ^b	145.3±43.15^b
	NB	1 230.02±58.74^a	208.54±15.12 ^a	0	4.33±0.67 ^a	377.79±32.72^a	0.8±0.06^a	1 109.04±264.36 ^a	20.83±4.13 ^a	388.77±53.69^b	9.67±1.21 ^ab	125.72±24.16^b
	JTB8-2	1 318.32±123.47 ^a	185.66±15.45^a	0	6.19±1.30 ^a	429.49±63.58^a	0.77±0.01 ^a	860.63±80.24 ^a	28.43±5.71 ^a	584.65±69.52^a	6.24±1.00 ^a	303.16±19.00^a
30	CK	2 061.35±266.97 ^a	122.59±10.45^b	0^b	1.84±0.29 ^b	180.08±11.35^a	0.84±0.05 ^a	1 315.59±87.63 ^a	20.05±1.70 ^a	765.03±46.15^a	11.54±2.31 ^a	1 004.80±219.91 ^a
	NB	1 794.80±141.57 ^a	177.72±7.90^a	0^b	3.29±0.16 ^a	230.88±86.56^a	0.77±0.05 ^a	841.93±145.93 ^a	20.85±4.98 ^a	368.51±110.82 ^b	3.32±0.39 ^b	327.14±80.60^b
	JTB8-2	1 942.68±230.51 ^a	169.77±15.11^a	6.82±1.21^a	3.57±0.16 ^a	307.98±63.41^a	0.83±0.02 ^a	1 292.95±432.77 ^a	13.56±1.35 ^a	235.59±31.28 ^b	3.06±0.41 ^b	359.94±41.51^b
40	CK	1 685.18±225.68 ^a	143.83±15.81 ^ab	0	4.62±1.91 ^a	247±29.36 ^a	1.26±0.14 ^ab	1 087.71±332.90 ^a	75.11±15.16 ^a	86.96±14.54 ^a	9.21±2.18 ^a	102.1±4.94 ^ab
	NB	1 791.51±171.03 ^a	181.93±13.16^a	0	5.38±0.63 ^a	223.25±39.89^a	1.53±0.05 ^a	535.96±76.85^a	66.31±19.89^a	202.2567 ^a	15.38±1.79 ^a	79.87±12.55 ^b
	JTB8-2	1 823.99±172.58 ^a	126.34±10.53 ^b	0	2.51±0.02 ^a	157.78±10.06^a	1.08±0.06 ^b	388.13±36.23^a	29±0.91^a	1 034.6467 ^a	10.92±1.43 ^a	129.64±8.29^a

时间 Time (d)	处理 Treatments	1-氨基环丙烷羧酸 1-Aminocyclo propanecar boxylic acid	吲哚-3-乙酸 Indole-3- acetic acid	顺式-玉米素 Cis-Zeatin	异戊烯基腺苷 Isopentenyl adenosine	反式玉米素核苷 trans- Zeatin- riboside	褪黑素 Melatonine	水杨酸 Salicylic acid	3-吲哚甲醛 Indole- 3-carboxa ldehyde	茉莉酸 Jasmonic acid	二氢茉莉酸 Dihydrojas monic acid	脱落酸 Abscisic acid
3	CK	901.32±47.38^b	179.69±6.73^b	5.02±1.02 ^b	2.9±0.23^a	201.81±24.05^b	0.74±0.01 ^a	603.92±63.52^b	18.40±0.83 ^b	331.88±124.89 ^b	8.52±0.97 ^b	338.90±20.61^b
	NB	1 727.7±139.06^a	172.96±4.68^b	11.51±0.62 ^a	4.46±1.15 ^a	344.26±55.36^a	0.76±0.03 ^a	1 135.76±102.83 ^a	36.71±0.85 ^b	608.77±44.14^a	22.05±3.56 ^a	488.11±40.29^a
	JTB8-2	1 668.43±122.45 ^a	212.71±3.42^a	13.23±1.06 ^a	2.97±0.21 ^a	183.79±8.29^b	0.84±0.08 ^a	642.91±48.71^b	73.36±10.18^a	205.02±13.87^b	13.71±0.68 ^b	417.70±21.58^ab
20	CK	867.28±27.13^b	165.87±2.79 ^a	0	3.37±0.41 ^a	155.52±38.42^b	0.81±0.02 ^a	1 077.13±150.23 ^a	15.32±1.90 ^a	211.33±39.76 ^b	15.20±2.64 ^b	145.3±43.15^b
	NB	1 230.02±58.74^a	208.54±15.12 ^a	0	4.33±0.67 ^a	377.79±32.72^a	0.8±0.06^a	1 109.04±264.36 ^a	20.83±4.13 ^a	388.77±53.69^b	9.67±1.21 ^ab	125.72±24.16^b
	JTB8-2	1 318.32±123.47 ^a	185.66±15.45^a	0	6.19±1.30 ^a	429.49±63.58^a	0.77±0.01 ^a	860.63±80.24 ^a	28.43±5.71 ^a	584.65±69.52^a	6.24±1.00 ^a	303.16±19.00^a
30	CK	2 061.35±266.97 ^a	122.59±10.45^b	0^b	1.84±0.29 ^b	180.08±11.35^a	0.84±0.05 ^a	1 315.59±87.63 ^a	20.05±1.70 ^a	765.03±46.15^a	11.54±2.31 ^a	1 004.80±219.91 ^a
	NB	1 794.80±141.57 ^a	177.72±7.90^a	0^b	3.29±0.16 ^a	230.88±86.56^a	0.77±0.05 ^a	841.93±145.93 ^a	20.85±4.98 ^a	368.51±110.82 ^b	3.32±0.39 ^b	327.14±80.60^b
	JTB8-2	1 942.68±230.51 ^a	169.77±15.11^a	6.82±1.21^a	3.57±0.16 ^a	307.98±63.41^a	0.83±0.02 ^a	1 292.95±432.77 ^a	13.56±1.35 ^a	235.59±31.28 ^b	3.06±0.41 ^b	359.94±41.51^b
40	CK	1 685.18±225.68 ^a	143.83±15.81 ^ab	0	4.62±1.91 ^a	247±29.36 ^a	1.26±0.14 ^ab	1 087.71±332.90 ^a	75.11±15.16 ^a	86.96±14.54 ^a	9.21±2.18 ^a	102.1±4.94 ^ab
	NB	1 791.51±171.03 ^a	181.93±13.16^a	0	5.38±0.63 ^a	223.25±39.89^a	1.53±0.05 ^a	535.96±76.85^a	66.31±19.89^a	202.2567 ^a	15.38±1.79 ^a	79.87±12.55 ^b
	JTB8-2	1 823.99±172.58 ^a	126.34±10.53 ^b	0	2.51±0.02 ^a	157.78±10.06^a	1.08±0.06 ^b	388.13±36.23^a	29±0.91^a	1 034.6467 ^a	10.92±1.43 ^a	129.64±8.29^a

Metabolomics analysis of Bacillus velezensis JTB8-2 induced tomato antagonism towards Orobanche aegyptiaca based on defense enzyme

基于防御酶与代谢组学分析贝莱斯芽孢杆菌JTB8-2诱导番茄拮抗瓜列当机制

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