Effects of Alternaria alternata infection on active oxygen metabolism in different tissues of melon

doi:10.6048/j.issn.1001-4330.2024.06.012

Abstract

Abstract:

【Objective】 To study the change trend of ROS and the activity of active oxygen metabolizing enzymes in melon tissues during the onset of black spot disease of Jiashi melon and 86-1 melon after inoculation with Alternaria alternata and discuss the relationship between the disease development and the change trend of ROS and the activity of active oxygen metabolizing enzymes. 【Methods】 The melon fruits of Jiashi and 86-1 were used as experimental materials to study the incidence of melon fruits, and the changing trend of the generation and removal of reactive oxygen species (ROS) in melon fruits when Alternaria alternata was injured and inoculated. 【Results】 After melon fruit was infected by Alternaria alternata, the contents of reactive oxygen species (H₂O₂ and O^2-) increased rapidly, and the activities of SOD and CAT increased, which reduced the production of MDA.The activities of SOD and CAT increased slowly in the early stage of infection, increased rapidly in the middle stage of infection, and decreased rapidly in the late stage of infection.During the whole storage period, the activities of SOD and CAT in Jiashi melon were higher than those in 86-1 melon, and the activities of SOD and CAT in the peel of both melons were higher than those in the pulp, and the activities of SOD and CAT in the two melon inoculation groups were higher than those in the control group. 【Conclusion】 During the storage period, the level of reactive oxygen species metabolism was closely related to the ability of the melon to defend against Alternaria alternate. The resistance of Jiashi melon is stronger against Alternaria alternate than that of 86-1 melon, especially in the middle and late storage.The disease resistance of Jiashi melon is stronger than that of 86-1 melon, and the skin disease resistance is stronger than that of pulp

Key words: muskmelon; Alternaria alternata; infection; reactive oxygen species

摘要：

【目的】研究甜瓜损伤接种链格孢菌后,伽师瓜和86-1甜瓜黑斑病发病期间,甜瓜组织内ROS、活性氧代谢酶活性的变化趋势,分析病情与ROS的变化规律以及活性氧代谢酶活性的变化特性。【方法】以伽师瓜和86-1甜瓜果实为材料,研究采后甜瓜损伤接种链格孢菌后,甜瓜果实中不同组织发病情况,分析甜瓜果实活性氧(ROS)产生和清除的变化趋势。【结果】甜瓜果实被链格孢菌侵染后,果实中活性氧H₂O₂和超氧阴离子含量迅速增加,提高SOD和CAT活性,减少了MDA的产生。侵染早期SOD和CAT活性含量上升缓慢、侵染中期迅速上升、侵染后期快速下降。整个贮藏期内,伽师瓜的SOD、CAT酶活性均比86-1甜瓜强,2种甜瓜果皮中SOD和CAT酶活性均比果肉中高,2种甜瓜接种组的SOD和CAT活性均比对照组高。【结论】甜瓜损伤接种链格孢菌后,在贮藏期间活性氧代谢水平与甜瓜抵御链格孢菌的能力关系密切。伽师瓜对链格孢菌抗性比86-1甜瓜强,尤其在贮藏中后期表现明显,伽师瓜抗病性强于86-1甜瓜,果皮抗病性强于果肉。

关键词: 甜瓜, 链格孢菌, 侵染, 活性

CLC Number:

S188

LIU Yang, ZHANG Zhengxiao, BAI Yujia, FENG Zuoshan. Effects of Alternaria alternata infection on active oxygen metabolism in different tissues of melon[J]. Xinjiang Agricultural Sciences, 2024, 61(6): 1397-1406.

刘阳, 张郑啸, 白羽嘉, 冯作山. 链格孢菌侵染对甜瓜不同组织活性氧代谢的影响[J]. 新疆农业科学, 2024, 61(6): 1397-1406.

Figures/Tables 16

Fig.1 Changes of disease incidenceof different melon varieties

Fig.2 Changes of A.alternata infection on O2- production rate of epricarp of melon

Fig.3 Changes of A.alternata infection on O2- production rate of endocarp of melon

Fig.4 Changes of A.alternata infection on O2- production rate of melon pulp

Fig.5 Changes of A.alternata infection on H2O2 content of epricarp of melon

Fig.6 Changes of A.alternata infection on H2O2 content of endocarp of melon

Fig.7 Changes of A.alternata infection on H2O2 content of melon pulp

Fig.8 Changes of A.alternata infection on MDA content of epricarp of melon

Fig.9 Changes of A.alternata infection on MDA content of endocarp of melon

Fig.10 Changes of A.alternata infection on MDA content of melon pulp

Fig.11 Changes of A.alternata infection on SOD activity of epricarp of melon

Fig.12 Changes of A.alternata infection on SOD activity of endocarp of melon

Fig.13 Changes of A.alternata infection on SOD content of melon pulp

Fig.14 Changes of A.alternata infection on CAT activity of epricarp of melon

Fig.15 Changes of A.alternata infection on CAT activity of endocarp of melon

Fig.16 Changes of A.alternata infection on CAT content of melon pulp

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