Effect of artificial aging on the viability and chemical content of cotton seeds

doi:10.6048/j.issn.1001-4330.2025.06.003

Xinjiang Agricultural Sciences ›› 2025, Vol. 62 ›› Issue (6): 1318-1327.DOI: 10.6048/j.issn.1001-4330.2025.06.003

• Crop Genetics and Breeding·Cultivation Physiology·Physiology and Biochemistry • Previous Articles Next Articles

Effect of artificial aging on the viability and chemical content of cotton seeds

DU Zehui¹(), GU Yuanguo², JIANG Zhengwen¹, GUO Yaping¹, WANG Yuxiang¹, DENG Xiaojuan¹()

1. College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China
2. Cotton Research Institute, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830052, China

Received:2024-11-09 Online:2025-06-20 Published:2025-07-29
Correspondence author: DENG Xiaojuan
Supported by:
Xinjiang Uygur Autonomous Region Colleges and Universities Basic Research Operating Expenses Scientific Research Project "Identification and Evaluation of Main Chemical Component Content of Cottonseed in Terrestrial Cotton Germplasm Resources and Mining of Oil Genes"(XJEDU2024J037);Xinjiang Qiangnong Fenghe Agricultural Science and Technology Co., Ltd. Horizontal Project, "GWAS-based Cottonseed Protein and Oil Gene Mining"(2523HXKT1);"Cotton Biological Breeding Innovation Project"(2021A02001-3)

人工老化对棉花种子活力和化学成分含量的影响

杜泽辉¹(), 顾元国², 蒋正文¹, 郭亚萍¹, 王宇翔¹, 邓晓娟¹()

1.新疆农业大学农学院,乌鲁木齐 830052
2.新疆维吾尔自治区农业科学院棉花研究所,乌鲁木齐 830052

通讯作者: 邓晓娟
作者简介:杜泽辉(1997-),男,浙江温州人,硕士,研究方向为作物遗传育种,(E-mail)245501176@qq.com
基金资助:
新疆维吾尔自治区高校基本科研业务费科研项目“陆地棉种质资源棉籽主要化学成分含量鉴定评价及油分基因挖掘”(XJEDU2024J037);新疆强农丰禾农业科技有限公司横向项目“基于GWAS的棉籽蛋白质和油分基因挖掘”(2523HXKT1);新疆维吾尔自治区重大专项“新疆主要农作物育种创新工程(一)”课题三“棉花生物育种创新工程”(2021A02001-3)

Abstract

Abstract:

【Objective】 Research the effect of artificial aging on cotton seed vitality and chemical composition content in the hope of providing reference for the utilization of high-vigor cotton seeds. 【Methods】 The seeds of eight Gossypium hirsutum L. varieties were taken as materials, the cotton seeds were artificially aged at 40℃ and 100% RH, and six treatments from 0 to 5 days were set up to determine the seed vigor indexes, physiological and biochemical indexes, and the content of the main chemical components of the seeds after drying naturally. 【Results】 And the results showed that, with the lengthening of the time of artificial aging the seed vigor firstly rose and then decreased, and the cell membrane permeability was constantly increasing and the activity of the protective enzymes was decreasing. 【Conclusion】 Different varieties of Gossypium hirsutum L. showed different degrees of antioxidant capacity, cell membrane stability, integrity, and seed vigor in resisting the aging process. In addition, there were significant correlations between cottonseed moisture content, protein content, oil content, etc. and germination rate, vigor index, conductivity, MDA content, etc.

Key words: Gossypium hirsutum L.; artificial aging; seed viability; chemical content

摘要：

【目的】研究人工老化对棉花种子活力和化学成分含量的影响,为高活力棉花种子利用提供参考。【方法】以8个陆地棉品种种子为材料,采用40℃、100%RH对棉籽进行人工老化处理,设置0~5 d 6个处理,自然干燥后测定种子活力指标、生理生化指标和种子主要化学成分含量。【结果】随着人工老化时间的加长种子活力先升高后降低,不断增加细胞膜透性、减少保护性酶活性。【结论】不同品种陆地棉在抵抗老化过程中展现出不同程度的抗氧化能力、细胞膜稳定性、完整性以及种子活力。此外,棉籽水分含量、蛋白质含量、油分含量等与发芽率、活力指数、电导率、MDA含量等指标之间存在显著的相关关系。

关键词: 陆地棉, 人工老化, 种子活力, 化学成分含量

CLC Number:

DU Zehui, GU Yuanguo, JIANG Zhengwen, GUO Yaping, WANG Yuxiang, DENG Xiaojuan. Effect of artificial aging on the viability and chemical content of cotton seeds[J]. Xinjiang Agricultural Sciences, 2025, 62(6): 1318-1327.

杜泽辉, 顾元国, 蒋正文, 郭亚萍, 王宇翔, 邓晓娟. 人工老化对棉花种子活力和化学成分含量的影响[J]. 新疆农业科学, 2025, 62(6): 1318-1327.

Figures/Tables 6

Fig.1 Changes of seed vitality index under artificial aging stress Notes: A: germination potential; B: germination rate; C: germination index; D: vigor index

Fig.2 Changes of seed growth indicators under artificial aging stress Notes: A: root length; B: seedling length

Fig.3 Changes of seed membrane permeability under artificial aging stress Notes: A: electrical conductivity; B: MDA content

Fig.4 Changes of seed protective enzyme activity under artificial aging stress Notes: A: POD activity; B: SOD activity

Fig.5 Changes of Seed chemical content analysis under artificial aging stress Figure Notes: A: moisture; B: free fatty acid; C: fat; D: protein

Fig.6 Changes of correlation analysis chart between seed vitality indicators and chemical components under artificial aging stress Notes: "**" at the 0.01 level (two-tailed), significant correlation, "*" at the 0.05 level (two-tailed), significant correlation

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Effect of artificial aging on the viability and chemical content of cotton seeds

人工老化对棉花种子活力和化学成分含量的影响

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