Mapping and genetic effect analysis of QTL for cotton seed oil content

doi:10.6048/j.issn.1001-4330.2025.05.005

Xinjiang Agricultural Sciences ›› 2025, Vol. 62 ›› Issue (5): 1084-1091.DOI: 10.6048/j.issn.1001-4330.2025.05.005

• Crop Genetics and Breeding·Cultivation Physiology·Physiology and Biochemistry • Previous Articles Next Articles

Mapping and genetic effect analysis of QTL for cotton seed oil content

DING Shugen¹(), SHI Yujie¹, Abudukeyoumu Abudurezike², XU Lin², WU Yuanlong¹, LI Zhibo¹, LIN Hairong¹, ZHAO Zengqiang³, NIE Xinhui¹()

1. Key Laboratory of Oasis Ecology/ Agricultural College of Shihezi University, Shihezi Xinjiang 832000, China
2. Laboratory of Quality and Safety Risk Assessment for Agro-Products, Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830000, China
3. Key Laboratory of Crop Germplasm Innovation and Gene Resource Utilization of XPCC / Cotton Research Institute of Xinjiang Academy of Agricultural Reclamation Sciences, Shihezi Xinjiang 832000, China

Received:2024-10-11 Online:2025-05-20 Published:2025-07-09
Correspondence author: NIE Xinhui
Supported by:
Creation of High Yield, High Quality, and High Oil Cotton Germplasm Resources(NYHXGG);Creation of High Yield, High Quality, and High Oil Cotton Germplasm Resources(2023AA102-2-1);Establishment of Molecular Assisted Selection Technology for New Cotton Varieties with Both Fiber and Feed(2023YFD2301203-05);Identification and Evaluation of Crop Germplasm Resources(BNZJ2023);Innovation of Cotton Genetic Resources and Its Application in Molecular Breeding(2021CB028)

棉花种子油分含量QTL的定位及遗传效应分析

丁树根¹(), 石玉杰¹, 阿布都克尤木·阿不都热孜克², 徐麟², 吴元龙¹, 李志博¹, 林海荣¹, 赵曾强³, 聂新辉¹()

1.石河子大学农学院 / 绿洲生态重点实验室,新疆石河子 832000
2.新疆维吾尔自治区农业科学院农业质量标准与检测技术研究所,乌鲁木齐 830000
3.新疆农垦科学院棉花研究所/作物种质创新与基因资源利用兵团重点实验室,新疆石河子 832000

通讯作者: 聂新辉
作者简介:丁树根(1998-),男,安徽人,硕士研究生,研究方向为棉花作物遗传育种,(E-mail)dsg199878@gmail.com
基金资助:
高产优质高油棉花种质资源的创制(NYHXGG);高产优质高油棉花种质资源的创制(2023AA102-2-1);纤饲兼用型棉花新品种分子辅助选择技术建立(2023YFD2301203-05);农作物种质资源鉴定与评价(BNZJ2023);棉花遗传资源创新及在分子育种中的应用(2021CB028)

Abstract

Abstract:

【Objective】 Based on SSR (Simple sequence repeat) markers, we aim to conduct correlation analysis on cotton SOC (Seed oil content), mine excellent allelic variation sites, and analyze the genetic mechanism of cotton seed oil content traits in the hope of providing a theoretical reference for cotton high-oil content breeding.【Methods】 145 pairs of SSR markers covering the entire cotton genome were screened to conduct polymorphism scanning on 245 cotton varieties; R language was used to draw population phenotype distribution and correlation diagrams, and the mixed linear model of TASSEL software was used to perform correlation analysis, and mine excellent allelic variation sites related to SOC.【Results】 34 allelic variation sites related to SOC were obtained (P<0.05). The explanation rate of phenotypic variation ranged from 1.83% to 8.7%, with an average value of 5.70%. 【Conclusion】 This study used correlation analysis to 34 sites related to oil content and cotton fiber quality and yield were discovered 9 sites.

Key words: SSR; seed oil content; correlation analysis

摘要：

【目的】基于SSR(Simple sequence repeat,简单重复序列)标记对棉花SOC(Seed oil content,种子油分含量)进行关联分析,挖掘优异等位变异位点,解析棉花种子油分含量性状的遗传机理,为棉花高油分育种提供理论参考。【方法】利用筛选出覆盖棉花全基因组的145对SSR标记对245份棉花材料进行多态性扫描;利用R语言绘制群体表型分布和相关关系图,采用TASSEL软件的混合线性模型进行关联分析,挖掘与SOC相关的优异等位变异位点。【结果】获得34个与SOC相关的等位变异位点(P<0.05)。表型变异解释率为1.83%~8.7%,平均值为5.70%。【结论】挖掘到34个与油分含量性状相关等位变异位点,以及棉花纤维品质和产量相关的位点9个。

关键词: SSR, 油分含量, 关联分析

CLC Number:

S562

DING Shugen, SHI Yujie, Abudukeyoumu Abudurezike, XU Lin, WU Yuanlong, LI Zhibo, LIN Hairong, ZHAO Zengqiang, NIE Xinhui. Mapping and genetic effect analysis of QTL for cotton seed oil content[J]. Xinjiang Agricultural Sciences, 2025, 62(5): 1084-1091.

丁树根, 石玉杰, 阿布都克尤木·阿不都热孜克, 徐麟, 吴元龙, 李志博, 林海荣, 赵曾强, 聂新辉. 棉花种子油分含量QTL的定位及遗传效应分析[J]. 新疆农业科学, 2025, 62(5): 1084-1091.

Figures/Tables 7

References 28

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环境 Environments	最小值 Min(%)	最大值 Max(%)	均值 Mean(%)	标准差 SD	变异系数 CV(%)	广义遗传力 H²(%)
2018年库尔勒 Korla in 2018	13.41	21.98	17.70	3.29	5.38	96.98
2019年库尔勒 Korla in 2019	14.45	21.76	17.79	1.93	9.22
2018年石河子 Shihezi 2018	13.96	21.05	17.43	1.86	9.37
2019年石河子 Shihezi 2019	14.17	19.67	16.84	1.59	10.59

环境 Environments	最小值 Min(%)	最大值 Max(%)	均值 Mean(%)	标准差 SD	变异系数 CV(%)	广义遗传力 H²(%)
2018年库尔勒 Korla in 2018	13.41	21.98	17.70	3.29	5.38	96.98
2019年库尔勒 Korla in 2019	14.45	21.76	17.79	1.93	9.22
2018年石河子 Shihezi 2018	13.96	21.05	17.43	1.86	9.37
2019年石河子 Shihezi 2019	14.17	19.67	16.84	1.59	10.59

性状 Trait	位点 Locus	P_FDR	表型变异解释率 Phenotypic variation explanatiory rateR²(%)
	HAU4483b^**	2.38E-06	8.70
	HAU4483a^**	6.10E-06	8.68
	MON_SHIN-1585a^**	6.29E-06	7.54
	MON_SHIN-1481a^**	1.14E-05	7.32
	MON_CGR5447a^**	1.96E-05	7.00
	HAU4022b^**	8.97E-05	6.93
	MUSS422aa^**	1.45E-04	6.90
SOC	HAU3071ad^**	3.18E-04	6.82
种子油分含量	HAU0875^**	0.0034	6.69
	NAU3346ba^**	0.0036	6.65
	MON_DPL0375ca^**	0.0042	6.61
	HAU-SNP113b^**	0.0055	6.54
	NAU3774a^**	0.0061	6.54
	NAU3736bb^*	0.0241	6.38
	HAU2588a^*	0.0285	6.37
	HAU2588b^*	0.0368	6.36
	NAU3827b^*	0.0395	6.30
	MUSS422ab^*	0.0432	6.06
	NAU5172b^*	0.029	5.18
	HAU-SNP113a^*	0.0362	5.14
	NBRI_HQ526730c^*	0.0422	5.00
	NBRI_HQ527566^*	0.0121	5.00
	DPL0062^*	0.0183	4.98
	HAU4022c^*	0.0224	4.84
SOC	BNL2449b^*	0.0237	4.83
种子油分含量	BNL2449a^*	0.029	4.76
	HAU1952bc^*	0.0368	4.71
	NAU7182^*	0.0395	4.55
	NAU3774b^*	0.0432	4.47
	MON_DPL0893a^*	0.0493	4.46
	BNL2535ba^*	0.0496	4.45
	BNL2535bb^**	1.14E-05	3.06
	NBRI_HQ527820a^**	1.96E-05	2.06
	MON_DPL0502a^**	8.97E-05	1.83

性状 Trait	位点 Locus	P_FDR	表型变异解释率 Phenotypic variation explanatiory rateR²(%)
	HAU4483b^**	2.38E-06	8.70
	HAU4483a^**	6.10E-06	8.68
	MON_SHIN-1585a^**	6.29E-06	7.54
	MON_SHIN-1481a^**	1.14E-05	7.32
	MON_CGR5447a^**	1.96E-05	7.00
	HAU4022b^**	8.97E-05	6.93
	MUSS422aa^**	1.45E-04	6.90
SOC	HAU3071ad^**	3.18E-04	6.82
种子油分含量	HAU0875^**	0.0034	6.69
	NAU3346ba^**	0.0036	6.65
	MON_DPL0375ca^**	0.0042	6.61
	HAU-SNP113b^**	0.0055	6.54
	NAU3774a^**	0.0061	6.54
	NAU3736bb^*	0.0241	6.38
	HAU2588a^*	0.0285	6.37
	HAU2588b^*	0.0368	6.36
	NAU3827b^*	0.0395	6.30
	MUSS422ab^*	0.0432	6.06
	NAU5172b^*	0.029	5.18
	HAU-SNP113a^*	0.0362	5.14
	NBRI_HQ526730c^*	0.0422	5.00
	NBRI_HQ527566^*	0.0121	5.00
	DPL0062^*	0.0183	4.98
	HAU4022c^*	0.0224	4.84
SOC	BNL2449b^*	0.0237	4.83
种子油分含量	BNL2449a^*	0.029	4.76
	HAU1952bc^*	0.0368	4.71
	NAU7182^*	0.0395	4.55
	NAU3774b^*	0.0432	4.47
	MON_DPL0893a^*	0.0493	4.46
	BNL2535ba^*	0.0496	4.45
	BNL2535bb^**	1.14E-05	3.06
	NBRI_HQ527820a^**	1.96E-05	2.06
	MON_DPL0502a^**	8.97E-05	1.83

位点 Locus	染色体 Chr	物理位置 Location (bp)	已报道研究性状及文献 Reported traits and references
HAU3071	Chr14	307	FE^[20]
NAU3346	Chr15	305	FL^[20]
NAU3736	Chr01	203	FE^[21]
NAU3827	Chr18	136	LY^[21]
NAU3774	Chr26	293	FU^[22],BW^[23], MV^[24],LW^[23-24]
DPL0062	Chr21	137	LW^[18], FUHML^[18], FU^[18],MV^[23-24]
MUSS422	Chr01	207	FU^[25], SFC^[25]
BNL2449	Chr13	183	BW^[18], FUHML^[18], MV^[18], FE^[18], LP^[26],FU^[27]
NBRI_HQ526730	Chr01	228	SI^[28],FE^[28]

Mapping and genetic effect analysis of QTL for cotton seed oil content

棉花种子油分含量QTL的定位及遗传效应分析

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