SSR fingerprinting and genetic diversity analysis of 26 fresh jujube cultivars

doi:10.6048/j.issn.1001-4330.2023.07.013

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (7): 1671-1678.DOI: 10.6048/j.issn.1001-4330.2023.07.013

• Horticultural Special Local Products·Storage and Preservation Processing • Previous Articles Next Articles

SSR fingerprinting and genetic diversity analysis of 26 fresh jujube cultivars

ZHANG Yanfei¹^,²(), Subina Xaokelaiti¹, YANG Lei¹, HAO Qing¹, JIN Juan¹(), FAN Dingyu¹()

1. Scientific Observing and Experimental Station of Pomology (Xinjiang),Ministry of Agriculture and Rural Affairs of the P.R.C. / Research Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
2. College of Horticulture, Xinjiang Agricultural University, Urumqi 830052, China

Received:2022-10-10 Online:2023-07-20 Published:2023-07-11
Correspondence author: JIN Juan (1990-), female, assistant researcher in Xuzhou, Jiangsu Province, research direction on fruit tree quality regulation and molecular biology of adversity, (E-mail)1475063956@qq.com；
FAN Dingyu (1983-), male, Xinzhou, Shanxi, associate researcher, research direction is reproductive development and hormone regulation of Chinese jujube, (E-mail)fandingyu2413@163.com
Supported by:
The S & T Assisting Xinjiang Program Project "Excellent Fresh Jujube 'Jingcang 1' Introduction and Supporting Cultivation Technology Integration Demonstration"(2020E0212);The Central Financial Forestry Reform and Development Fund Project "Demonstration and Promotion of High-quality and Efficient Cultivation Technology of New Fresh Jujube Varieties"(Xin[2020]TG17)

26个鲜食枣品种SSR指纹图谱构建与遗传多样性分析

张雁飞¹^,²(), 苏比娜·肖克来提¹, 杨磊¹, 郝庆¹, 靳娟¹(), 樊丁宇¹()

1.新疆农业科学院园艺作物研究所/农业部新疆地区果树科学观测实验站,乌鲁木齐 830091
2.新疆农业大学园艺学院,乌鲁木齐 830052

通讯作者: 靳娟(1990-),女,江苏徐州,助理研究员,研究方向果树品质调控及逆境分子生物学,(E-mail)1475063956@qq.com；
樊丁宇(1983-),男,山西忻州人,研究员,研究方向为果树栽培与生理,(E-mail)fandingyu2413@163.com
作者简介:张雁飞(1997-),女,甘肃靖远人,硕士研究生,研究方向为果树栽培与生理,(E-mail)2453118791@qq.com
基金资助:
新疆维吾尔自治区科技支疆项目“优良鲜食枣‘京沧1号’引进及配套栽培技术集成示范”(2020E0212);中央财政林业改革发展资金项目“鲜食枣新品种优质高效栽培技术示范与推广”(新[2020]TG17)

Abstract

Abstract:

【Objective】 This experiment aims to construct the fingerprint map on 26 fresh jujube germplasm resources and analyze their genetic diversity in the hope of providing a reference for fresh jujube variety identification and genetic diversity analysis.【Methods】 A total of 26 fresh jujube varieties were analyzed using 22 SSR primers and polymorphic by fluorescent M13 capillary electrophoresis that amplified 130 allelic fragments with an average of 5.909.【Results】 The polymorphic information content (PIC) varied from 0.237,7 to 0.855,6, with an average of 0.59.The heterozygosity for 22 SSR primers ranged from 0.500 to 1.000, with an average of 0.927.The combination of three primer pairs, BFU0308, BFU1157 and BFU0574, could completely distinguish the 26 jujube varieties.【Conclusion】 The genetic similarity coefficient of the 26 varieties is between 0.65 and 1.00, and the 26 varieties can be divided into two major groups at 0.65.

Key words: fresh jujube; SSR; capillary electrophoresis; fingerprint

摘要：

【目的】研究26份鲜食枣种质资源构建指纹图谱,分析遗传多样性,为鲜食枣品种鉴定和遗传多样性分析提供参考依据。【方法】利用22对SSR引物对26个鲜食枣品种进行分析,采用荧光M13毛细管电泳技术进行多态检测,共扩增出130条等位片段,平均5.909个。【结果】多态信息含量(PIC)变化范围为0.237 7~0.855 6,平均为0.59。22对SSR引物的杂合度介于0.500~1.000,平均为0.927。3对引物BFU0308,BFU1157和BFU0574组合能完全区分26个枣品种。【结论】26个品种遗传相似系数在0.65~1.00,相似系数0.65处可将26个品种分为两大类群。

关键词: 鲜食枣, SSR, 毛细管电泳, 指纹图谱

CLC Number:

S665.1

ZHANG Yanfei, Subina Xaokelaiti, YANG Lei, HAO Qing, JIN Juan, FAN Dingyu. SSR fingerprinting and genetic diversity analysis of 26 fresh jujube cultivars[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1671-1678.

张雁飞, 苏比娜·肖克来提, 杨磊, 郝庆, 靳娟, 樊丁宇. 26个鲜食枣品种SSR指纹图谱构建与遗传多样性分析[J]. 新疆农业科学, 2023, 60(7): 1671-1678.

Figures/Tables 5

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编号 Code	品种名称 Cultiver name	来源 Origin	形态特征 Main characteristics
1	京沧1号	河北	果实大,扁圆形,果皮脆薄,赭红色,中熟
2	雨娇	河北	果实近圆形,果皮中厚,赭红色,中晚熟
3	金脆蜜	山西	果实椭圆形,果皮薄,金黄色,极早熟
4	早秋红	山东	果实近圆形,果皮薄,鲜红色,早熟
5	赛蜜酥1号	新疆	果实近圆形,果皮薄,鲜红色
6	京沧8号	河北	果实大,近圆形,果皮薄,赭红色
7	阜帅	河北	果实近圆形,果品中厚,赭红色
8	秦820	陕西	果实近圆形,果皮脆薄,赭红色,早熟
9	早脆王	河北	果实卵圆形,果皮鲜红
10	迎秋红	山东	果实倒卵圆形,果皮薄,鲜红色,早熟
11	早冬1号	山东	果实近圆形,果皮脆薄,赭红色,早熟
12	月光	河北	果实近橄榄形,果皮脆薄,赭红色
13	阜香	河北	果实长圆形,果皮赭红色
14	香脆	山西	果实近圆形,果皮赭红色
15	金芒果枣	新疆	果实圆柱形,果皮薄,赭红色
16	冬枣2号	河北	果实近圆形,果皮脆薄,赭红色
17	京沧6号	河北	果实圆锥形,果皮薄,赭红色
18	伏脆蜜	山东	果实短圆柱形,果皮脆薄,鲜红色
19	ZS 19	河北	果实葫芦形或子弹形,果皮赭红色
20	圆铃2号	山东	果实短圆形,果皮中厚,亮紫红色
21	蜂蜜罐	陕西	果实近圆形,果皮薄,鲜红色
22	京枣39	河北	果实圆柱形,果皮薄,赭红色
23	雨帅	河北	果实长圆形,果皮赭红色
24	美蜜枣	河北	果实圆柱形,果皮红色
25	懒汉冬枣	山东	果实近圆形,果皮薄,赭红色
26	迎白露	山东	果实近圆形,果皮红色

编号 Code	品种名称 Cultiver name	来源 Origin	形态特征 Main characteristics
1	京沧1号	河北	果实大,扁圆形,果皮脆薄,赭红色,中熟
2	雨娇	河北	果实近圆形,果皮中厚,赭红色,中晚熟
3	金脆蜜	山西	果实椭圆形,果皮薄,金黄色,极早熟
4	早秋红	山东	果实近圆形,果皮薄,鲜红色,早熟
5	赛蜜酥1号	新疆	果实近圆形,果皮薄,鲜红色
6	京沧8号	河北	果实大,近圆形,果皮薄,赭红色
7	阜帅	河北	果实近圆形,果品中厚,赭红色
8	秦820	陕西	果实近圆形,果皮脆薄,赭红色,早熟
9	早脆王	河北	果实卵圆形,果皮鲜红
10	迎秋红	山东	果实倒卵圆形,果皮薄,鲜红色,早熟
11	早冬1号	山东	果实近圆形,果皮脆薄,赭红色,早熟
12	月光	河北	果实近橄榄形,果皮脆薄,赭红色
13	阜香	河北	果实长圆形,果皮赭红色
14	香脆	山西	果实近圆形,果皮赭红色
15	金芒果枣	新疆	果实圆柱形,果皮薄,赭红色
16	冬枣2号	河北	果实近圆形,果皮脆薄,赭红色
17	京沧6号	河北	果实圆锥形,果皮薄,赭红色
18	伏脆蜜	山东	果实短圆柱形,果皮脆薄,鲜红色
19	ZS 19	河北	果实葫芦形或子弹形,果皮赭红色
20	圆铃2号	山东	果实短圆形,果皮中厚,亮紫红色
21	蜂蜜罐	陕西	果实近圆形,果皮薄,鲜红色
22	京枣39	河北	果实圆柱形,果皮薄,赭红色
23	雨帅	河北	果实长圆形,果皮赭红色
24	美蜜枣	河北	果实圆柱形,果皮红色
25	懒汉冬枣	山东	果实近圆形,果皮薄,赭红色
26	迎白露	山东	果实近圆形,果皮红色

引物名称 Primer name	连锁群 LG	正向引物(5'-3') Forward primer	反向引物(5'-3') Reverse primer	退火温度 Annealing temperature(℃)
BFU0263	1	GGTTTTTGTGGGTATGGAGGT	AGGAAAACAAGGGGATGGAGA	55
BFU0478	8	AACGCTGAAGATTTCCTCCTC	CCTGAATTCCAACCAAAACAG	55
BFU1205	6	TGTTGCTGGTTCAATTCCAG	CTTATGGCTTTTTCATTTTGTGA	55
BFU0586	9	CGAACTTGGAGAGCTTGGAG	TTGAGCTCTGCAACGAAATG	55
BFU0377	2	CCAGCTGGTATCCAATTGCT	ACGACGATGCCATGAAAGAT	55
BFU0539	4	CCGGAAACGTTTAAAATGACA	GGAGGAAGAAGGATCCAAGG	55
BFU1279	1	TTTTTCAAGACCTCCACGATG	TCCCACCACTTTCCTCTCAT	55
BFU0249	3	AATGGGTCCACGTAGACAGG	GCCCTGAGGTTGGACATAGA	55
BFU0733	9	TCCTTTTGCCGAGAATATGAA	GTGAAGCCCCTAATTGTGTCA	55
BFU0467	3	CCGGACCGAGTGGAGTTATTA	AGAATATGGCATCAACCTATACCA	55
BFU0308	1	TTTCCACCCCAAAATACCAA	AGACGCTGGATGAGGATGAT	55
BFU0584	2	AGGTCGATTTCCCCATCAC	GCTGAGAGAGAATCCCAACG	55
BFU0473	3	GTCCTGATGTGGAGTGCATTT	TCTACAAGGACGAATCGTTGC	55
BFU1157	2	TCCCTAAATTACCCTTCCCAAT	AAAGCGACAGCGAAAACTGT	55
BFU0501	3	GCCATGCTTGACTTGCTACA	AATGTTCCCATCCTCCCTTC	55
BFU0614	1	GATCGGTCCGAGACGATAAA	ATACGCTCACGCCCTAGTGT	55
BFU1178	2	CCTTGGTGGATTTTGGTTTG	TATACTTTGGCAGCGGTGTG	55
BFU0479	1	GAAAACCATTGTTGGAGACCA	TGAACCAAGCAACAAAAATCA	55
BFU0574	1	GAAGGTTGAAGATGCTCTCTCTC	CCTGACATCCATTTGAAGGAA	55
BFU0521	9	CCTTTACTCGGCATTTCCAA	TGGTGAAGCAGCAAAAACAG	55
BFU1409	1	AAATGATGGATCGAGCAAA	AATGGAGGACAAACCGTCAC	55
BFUO277	6	GCACTACCCTGTGGAACTCAA	GTGTTGACCTGGCAAGAAGA	55

引物名称 Primer name	连锁群 LG	正向引物(5'-3') Forward primer	反向引物(5'-3') Reverse primer	退火温度 Annealing temperature(℃)
BFU0263	1	GGTTTTTGTGGGTATGGAGGT	AGGAAAACAAGGGGATGGAGA	55
BFU0478	8	AACGCTGAAGATTTCCTCCTC	CCTGAATTCCAACCAAAACAG	55
BFU1205	6	TGTTGCTGGTTCAATTCCAG	CTTATGGCTTTTTCATTTTGTGA	55
BFU0586	9	CGAACTTGGAGAGCTTGGAG	TTGAGCTCTGCAACGAAATG	55
BFU0377	2	CCAGCTGGTATCCAATTGCT	ACGACGATGCCATGAAAGAT	55
BFU0539	4	CCGGAAACGTTTAAAATGACA	GGAGGAAGAAGGATCCAAGG	55
BFU1279	1	TTTTTCAAGACCTCCACGATG	TCCCACCACTTTCCTCTCAT	55
BFU0249	3	AATGGGTCCACGTAGACAGG	GCCCTGAGGTTGGACATAGA	55
BFU0733	9	TCCTTTTGCCGAGAATATGAA	GTGAAGCCCCTAATTGTGTCA	55
BFU0467	3	CCGGACCGAGTGGAGTTATTA	AGAATATGGCATCAACCTATACCA	55
BFU0308	1	TTTCCACCCCAAAATACCAA	AGACGCTGGATGAGGATGAT	55
BFU0584	2	AGGTCGATTTCCCCATCAC	GCTGAGAGAGAATCCCAACG	55
BFU0473	3	GTCCTGATGTGGAGTGCATTT	TCTACAAGGACGAATCGTTGC	55
BFU1157	2	TCCCTAAATTACCCTTCCCAAT	AAAGCGACAGCGAAAACTGT	55
BFU0501	3	GCCATGCTTGACTTGCTACA	AATGTTCCCATCCTCCCTTC	55
BFU0614	1	GATCGGTCCGAGACGATAAA	ATACGCTCACGCCCTAGTGT	55
BFU1178	2	CCTTGGTGGATTTTGGTTTG	TATACTTTGGCAGCGGTGTG	55
BFU0479	1	GAAAACCATTGTTGGAGACCA	TGAACCAAGCAACAAAAATCA	55
BFU0574	1	GAAGGTTGAAGATGCTCTCTCTC	CCTGACATCCATTTGAAGGAA	55
BFU0521	9	CCTTTACTCGGCATTTCCAA	TGGTGAAGCAGCAAAAACAG	55
BFU1409	1	AAATGATGGATCGAGCAAA	AATGGAGGACAAACCGTCAC	55
BFUO277	6	GCACTACCCTGTGGAACTCAA	GTGTTGACCTGGCAAGAAGA	55

引物名称 Primer name	等位基因数目 Number of alleles	PIC值 PIC Value	Na	Ne	I	Ho	He
BFU0263	2	0.365 6	2.000	1.929	0.675	0.654	0.482
BFU0478	4	0.600 7	4.000	2.889	1.199	0.500	0.654
BFU1205	7	0.793 2	7.000	5.496	1.787	0.846	0.818
BFU0586	7	0.665 6	7.000	3.476	1.463	0.769	0.712
BFU0377	6	0.674 2	6.000	3.577	1.420	0.923	0.720
BFU0539	7	0.584 4	7.000	2.828	1.292	1.000	0.646
BFU1279	10	0.831 4	10.000	6.627	2.045	1.000	0.849
BFU0249	7	0.758 9	7.000	4.711	1.727	1.000	0.788
BFU0733	3	0.577 8	3.000	2.834	1.070	1.000	0.647
BFU0467	8	0.748 7	8.000	4.583	1.688	1.000	0.782
BFU0308	10	0.855 6	10.000	7.638	2.142	0.962	0.869
BFU0584	4	0.429 2	4.000	2.052	0.866	1.000	0.513
BFU0473	5	0.510 9	5.000	2.224	1.082	1.000	0.550
BFU1157	9	0.827 6	9.000	6.531	1.993	1.000	0.847
BFU0501	3	0.514 0	3.000	2.368	0.975	1.000	0.578
BFU0614	2	0.278 3	2.000	1.501	0.516	0.731	0.334
BFU1178	4	0.642 9	4.000	3.338	1.269	1.000	0.700
BFU0479	7	0.768 2	7.000	4.971	1.694	1.000	0.799
BFU0574	8	0.775 7	8.000	4.934	1.830	1.000	0.797
BFU0521	5	0.237 7	5.000	1.325	0.567	1.000	0.246
BFU1409	5	0.611 6	5.000	3.025	1.243	1.000	0.669
BFUO277	7	0.731 2	7.000	4.225	1.620	1.000	0.763
总计Total	130
平均Mean	5.909	0.59	5	3.776	1.371	0.927	0.671

SSR fingerprinting and genetic diversity analysis of 26 fresh jujube cultivars

26个鲜食枣品种SSR指纹图谱构建与遗传多样性分析

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品种编号Cultiver code	指纹图谱Fingerprint
1	BFU0308,136,151/BFU1157,217,234/BFU0574,89,120
2	BFU0308,151,157/BFU1157,226,232/BFU0574,89,95
3	BFU0308,136,151,171/BFU1157,217,232/BFU0574,89,121
4	BFU0308,149,173/BFU1157,217,219/BFU0574,89,95
5	BFU0308,135,175/BFU1157,230,232/BFU0574,89,121
6	BFU0308,135,151,157/BFU1157,205,230/BFU0574,89,103
7	BFU0308,151,157/BFU1157,205,230/BFU0574,89,111
8	BFU0308,135,175/BFU1157,226,234/BFU0574,89,121
9	BFU0308,149,173/BFU1157,217,218/BFU0574,89
10	BFU0308,151,165,171/BFU1157,217,234/BFU0574,89
11	BFU0308,135,175/BFU1157,226,234/BFU0574,89
12	BFU0308,157,165/BFU1157,230,234/BFU0574,89,111
13	BFU0308,135,151/BFU1157,226,230/BFU0574,89,121
14	BFU0308,135,171/BFU1157,226,232/BFU0574,89,11
15	BFU0308,151/BFU1157,217,232/BFU0574,89,119
16	BFU0308,135,175/BFU1157,226,234/BFU0574,89,119
17	BFU0308,157,171/BFU1157,230,232/BFU0574,95,103
18	BFU0308,135,175/BFU1157,230,232/BFU0574,95,121
19	BFU0308,171/BFU1157,232,234/BFU0574,95,103
20	BFU0308,157,175/BFU1157,217,230/BFU0574,103,111
21	BFU0308,171,177/BFU1157,226,230/BFU0574,111,121
22	BFU0308,157,165,171/BFU1157,217,234/BFU0574,111,119
23	BFU0308,173/BFU1157,219,230/BFU0574,118,120
24	BFU0308,149/BFU1157,219,232/BFU0574,119,121
25	BFU0308,135,151,171/BFU1157,217,232/BFU0574,119,121
26	BFU0308,135,171/BFU1157,226,232/BFU0574,119,121

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