Study on Application Technology of Bacillus amyloliquefaciens B1619 in Promoting Healthy Growth of Tomato Seedlings

doi:10.6048/j.issn.1001-4330.2022.05.026

Abstract

Abstract:

【Objective】 To study the comprehensive effects of Bacillus amyloliquefaciens B1619 on growth promotion and disease prevention of processed tomato seedlings in the hope of providing a set of healthy, ecological and green management technology for the cultivation of robust seedlings and for the promotion of healthy development of processed tomato industry in Xinjiang. 【Method】 The effects of Bacillus amylolitica B1619 to growth of tomato seedlings and the prevention of seedling diseases were studied with matrix mixed medicine and spray method through laboratory experiment of hole dish seedling at seedling stage. 【Result】 The Bacillus amylolitica B1619 improves seedling emergence rate, promote growth, prevent and control damping off, its also beneficial to cultivate healthy seedlings, improve immunity, and reduce soil-borne diseases of tomato seedlings.1. Bacillus amylolitica B1619 improved the soil ecological environment of tomato seedling hole disk. The soil was loose, biomass of tomato root and seedling was significantly increased after application of bacillus compared with the control. The leaves were darker and the chlorophyll content increased. 2.Its improved immunity of tomato, enhanced disease resistance, and processed tomato seedlings had a strong role in the prevention of cataplexy. 【Conclusion】 B.amylum B1619 can improve the ecological environment of the biomass of tomato roots and seedlings; the leaf color deepens and the chlorophyll content increases. It can improve tomato immunity, enhance disease resistance, and have a strong preventive effect on cataplexy in processed tomato seedlings.

Key words: Bacillus amyloliquefaciens B1619; processed tomato; usage method; promotional role; disease control

摘要：

【目的】研究解淀粉芽孢杆菌Bacillus amyloliquefaciens B1619对加工番茄幼苗促生和苗期病害防控的综合作用,为培育壮苗,提供绿色的管理技术。【方法】采用室内穴盘育苗试验,分别用基质拌药和苗期喷雾法,研究解淀粉芽孢杆菌B1619对加工番茄幼苗生长的促进作用,以及对苗期病害的预防作用,研究其促生和防病的效果。【结果】各处理的出苗率达到稳定状态,呈现出现先抑制后促进的生长态势。1∶150出苗率最好、1∶100次之,1∶200出苗率较差,拌土法比喷雾法对番茄株高的促生作用更强。以1∶150拌土对番茄茎粗的促进作用最强。不同拌土量处理的叶绿素含量与对照之间具有极显著性差异,叶绿素含量比对照增加7.68%~10.20%,拌土法对幼苗猝倒病具有较强的预防作用,1∶150和1∶200拌土发病率只有0.00~0.08%。【结论】解淀粉芽孢杆菌B1619能够改善加工番茄育苗穴盘的土壤生态环境,施用后土壤蓬松,对番茄根系和幼苗的生物量比对照有显著性增加;叶片颜色较深,叶绿素含量增加。能够提高番茄免疫力,增强抗病性,对加工番茄幼苗猝倒病具有较强的预防作用。

关键词: 解淀粉芽孢杆菌B1619, 加工番茄, 使用方法, 促生作用, 病害控制

CLC Number:

S188

WANG Fei, QIU Guang, LUO Wenfang, SUN Xiaojun, YANG Hongmei, LIU Chengjun, XU Jianjun, HE Wei. Study on Application Technology of Bacillus amyloliquefaciens B1619 in Promoting Healthy Growth of Tomato Seedlings[J]. Xinjiang Agricultural Sciences, 2022, 59(5): 1252-1259.

汪飞, 邱光, 罗文芳, 孙晓军, 杨洪梅, 刘成军, 许建军, 何伟. 解淀粉芽孢杆菌B1619对加工番茄幼苗促生及培育壮苗的影响[J]. 新疆农业科学, 2022, 59(5): 1252-1259.

Figures/Tables 9

References 20

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处理 Treat ment	3 d出苗率 Seedling emergence rate after 3 d(%)	6 d出苗势 Seedling emergence rate after 6 d(%)	比CK±(%)
1∶100	88.20^ab	92.73±0.89^ab	1.37
1∶150	90.50^a	95.70 ±0.59^a	4.61
1∶200	75.55^b	89.92 ±1.65^b	- 1.71
0	87.73^ab	91.48 ± 1.13^ab	—

处理 Treat ment	3 d出苗率 Seedling emergence rate after 3 d(%)	6 d出苗势 Seedling emergence rate after 6 d(%)	比CK±(%)
1∶100	88.20^ab	92.73±0.89^ab	1.37
1∶150	90.50^a	95.70 ±0.59^a	4.61
1∶200	75.55^b	89.92 ±1.65^b	- 1.71
0	87.73^ab	91.48 ± 1.13^ab	—

处理 Treat ments	根鲜重 Root fresh weight(g)	比CK ±(%)	茎叶鲜重 Leaf and stem fresh weight (g)	比CK ±(%)	整株鲜重 Fresh weight (g)	比CK ±(%)	整株干重 Dry weight (g)	比CK ±(%)
1∶100	0.100^Bb	16.57	1.43^b	2.09	1.55^b	0.87	0.25^a	7.85
1∶150	0.124^Aa	43.60	1.68^a	20.02	1.73^a	12.66	0.25^a	7.9
1∶200	0.128^Aa	48.55	1.61^a	15.18	1.68^a	9.4	0.26^a	11.73
对照Control	0.086^Bb	/	1.40^b	/	1.54^b	/	0.23^a	/

处理 Treat ments	根鲜重 Root fresh weight(g)	比CK ±(%)	茎叶鲜重 Leaf and stem fresh weight (g)	比CK ±(%)	整株鲜重 Fresh weight (g)	比CK ±(%)	整株干重 Dry weight (g)	比CK ±(%)
1∶100	0.100^Bb	16.57	1.43^b	2.09	1.55^b	0.87	0.25^a	7.85
1∶150	0.124^Aa	43.60	1.68^a	20.02	1.73^a	12.66	0.25^a	7.9
1∶200	0.128^Aa	48.55	1.61^a	15.18	1.68^a	9.4	0.26^a	11.73
对照Control	0.086^Bb	/	1.40^b	/	1.54^b	/	0.23^a	/

处理方式 Treatments		叶绿素 (Spad) chlorophyll	比CK± (%)	处理方式 Treatments		叶绿素 (Spad) chlorophyll	比CK± (%)
用量 Dosage	方法 Methods	叶绿素 (Spad) chlorophyll	比CK± (%)	用量 Dosage	方法 Methods	叶绿素 (Spad) chlorophyll	比CK± (%)
20倍	喷雾	37.92^a	-0.71	1:100	拌土	56.83^Aa	10.20
30倍	喷雾	37.67^a	-1.36	1:150	拌土	56.00^Aa	8.60
40倍	喷雾	39.44^a	3.27	1:200	拌土	55.53^Aa	7.68
CK		38.19^a	/	对照		51.57^Bb