Effects of High Temperature Stress on Physiological Characteristics on Jujube Seedlings

doi:10.6048/j.issn.1001-4330.2018.03.006

Abstract

Abstract: 【Objective】 To explore the adaptation mechanism of sour and hui jujube seedlings to high temperature stress.【Method】Sour jujube and hui jujube seedlings were used as the materials, plug experiments were conducted to investigate the effects of 45℃ high temperature treatment at different time (0, 1, 3, 5 d) on relative conductivity, malonaldehyde, osmotic substances content, antioxidant enzyme activity and the photosynthetic pigments in leaves of sour jujube and huizao jujube seedlings.【Result】Leaf relative conductivity of sour jujube seedlings increased significantly only on the 5th-day high temperature treatment compared with the control treatment, which was 18.1% higher than that of the CK, but no significant differences were observed in leaf MDA content among all the treatments. The relative electrical conductivity and MDA content in leaves of Zizyphus jujuba seedlings increased significantly, and the cell membrane system was damaged seriously. As the high temperature treatment time prolonged, the soluble sugar content in leaves of sour jujube seedlings first decreased and then increased, proline, SOD and CAT activity significantly increased, the soluble sugar, SOD activity of hui jujube seedlings first decreased and then increased, proline and CAT activity significantly increased. High temperature treatment had no significant effect on leaf soluble protein, POD activity of sour jujube and hui jujube seedlings. The photosynthetic pigment content in the leaves of jujube and gray jujube seedlings was significantly decreased by high temperature treatment, and the decrease rate of photosynthetic pigment content in the leaves of wild jujube seedlings was smaller than that of gray jujube seedlings.【Conclusion】With the extension of high temperature stress time, soluble sugar, proline content, SOD and CAT activities in leaves of sour and hui jujube seedlings increased, and the content of photosynthetic pigments decreased. Moreover, the cell membrane stability of the leaves of jujube was stronger than that of the seedlings of Zizyphus jujuba, which showed that sour jujube seedlings had stronger heat resistance.

Key words: jujube; high temperature stress; physiological and biochemical indexes; photosynthetic pigment

摘要： 【目的】研究酸枣和灰枣实生苗对高温胁迫的适应机制。【方法】以酸枣和灰枣穴盘苗为材料,研究45℃高温处理不同时间(0、1、3、5 d)对枣叶片相对电导率、丙二醛、渗透调节物质含量、抗氧化酶活性以及光合色素含量的影响。【结果】高温处理不同时间下,酸枣苗叶片相对电导率仅在高温处理第5 d显著增加,较对照提高18.1%,叶片MDA含量变化不显著,灰枣实生苗叶片相对电导率和MDA含量显著升高,细胞膜系统受损严重。随着高温处理时间的延长,酸枣叶片可溶性糖含量先下降后上升,脯氨酸、SOD和CAT活性显著升高,灰枣实生苗叶片可溶性糖、SOD活性先下降后上升,脯氨酸和CAT活性显著升高。高温处理下酸枣和灰枣实生苗叶片可溶性蛋白、POD活性变化不大。高温处理显著降低酸枣和灰枣实生苗叶片光合色素含量,酸枣叶片光合色素含量下降幅度较灰枣实生苗小。【结论】随着高温胁迫时间的延长,酸枣和灰枣实生苗叶片可溶性糖、脯氨酸、SOD和CAT活性均提高,光合色素含量均下降,并且酸枣叶片细胞膜稳定性较灰枣实生苗强,光合色素下降幅度较灰枣实生苗小,耐热性较灰枣实生苗强。

关键词: 枣, 高温胁迫, 生理生化指标, 光合色素

CLC Number:

S665.1

JIN Juan, YANG Lei, FAN Ding-yu, HAO Qing. Effects of High Temperature Stress on Physiological Characteristics on Jujube Seedlings[J]. Xinjiang Agricultural Sciences, 2018, 55(3): 439-447.

靳娟,杨磊,樊丁宇,郝庆. 高温胁迫对枣苗生理特性的影响[J]. 新疆农业科学, 2018, 55(3): 439-447.

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