Electrophysiological responses of the Monolepta hieroglyphica (Motschulsky) to 10 volatiles of cotton and maize

doi:10.6048/j.issn.1001-4330.2018.02.011

Abstract

Abstract: 【Objective】 To screen plant volatiles with biological activity against Monolepta hieroglyphica (Motschulsky) adults.【Method】The volatile compounds of cotton and maize were identified and quantitatively analyzed by gas chromatography and mass spectrometry (GC-MS). The electroantennogram (EAG) technique was used to determine the electrophysiological responses of the female and male Monolepta hieroglyphica to 10 volatiles.【Result】The response of M. hieroglyphica male to Tricyclo, Dragosantol, α-Pinene, Myrcene, 3-Carene, 1,3-Butadiene, Palmitic acid were relatively obvious. The response of M. hieroglyphica female to 10 kinds of volatile with part concentration was relatively obvious.【Conclusion】The response of M. hieroglyphica males to 3-Carene is the most sensitive, the response of M. hieroglyphica females to Dragosantol is the most sensitive.

Key words: Monolepta hieroglyphica; cotton; corn; volatiles; electrophysiological response

摘要： 【目的】筛选对双斑长跗萤叶甲Monolepta hieroglyphica (Motschulsky)成虫有生物活性的植物挥发物。【方法】通过气相-质谱联用仪(GC-MS)对棉花和玉米挥发物进行鉴定及定量分析,采用触角电位技术 (EAG)测定双斑长跗萤叶甲雌、雄成虫对10种挥发物的触角电位反应。【结果】双斑长跗萤叶甲雄虫对α-长叶蒎烯、α-红没药醇、α-蒎烯、月桂烯、3-蒈烯、1,3丁二烯、棕榈酸反应较为明显;雌虫对10种挥发物部分浓度均有明显反应。【结论】双斑长跗萤叶甲雄虫对3-蒈烯反应最敏感,雌虫对α-红没药醇反应最敏感。

关键词: 双斑长跗萤叶甲, 棉花, 玉米, 挥发物, 触角电生理反应

CLC Number:

ZHANG Zhi-Hu, GUO Dan-Dan, CHEN Jing, HAN Xiao-Qiang. Electrophysiological responses of the Monolepta hieroglyphica (Motschulsky) to 10 volatiles of cotton and maize[J]. Xinjiang Agricultural Sciences, 2018, 55(2): 285-292.

张志虎,郭丹丹,陈静,韩小强. 双斑长跗萤叶甲对棉花和玉米10种挥发物的触角电生理反应[J]. 新疆农业科学, 2018, 55(2): 285-292.

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