棉田不同菌型棉蚜体内微生物多样性分析

doi:10.6048/j.issn.1001-4330.2024.09.023

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (9): 2277-2284.DOI: 10.6048/j.issn.1001-4330.2024.09.023

• 植物保护·微生物·畜牧兽医·土壤肥料 • 上一篇下一篇

棉田不同菌型棉蚜体内微生物多样性分析

安哲¹^,²(), 牛瑞昌², 朱香镇², 王丽², 张开心², 李东阳², 姬继超², 牛林², 高雪珂², 雒珺瑜², 崔金杰²(), 马德英¹()

1.新疆农业大学农学院/农林有害生物监测与安全防控重点实验室,乌鲁木齐 830052
2.中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳 455000

收稿日期:2024-02-20 出版日期:2024-09-20 发布日期:2024-10-09
通信作者: 马德英(1968-),女,新疆乌鲁木齐人,教授,博士,硕士生/博士生导师,研究方向为有害生物绿色防控,(E-mail)mdynd@163.com；
崔金杰(1968-),男,研究员,硕士生/博士生导师,研究方向为农业昆虫与害虫防治,(E-mail)Cuijinjie@126.com
作者简介:安哲(1996-),女,内蒙古乌兰察布人,硕士研究生,研究方向为农业昆虫与害虫防治,(E-mail)anzhe1206@126.com
基金资助:
中国农业科学院科技创新工程(ZB2021046)

Analyze the microbial diversity of cotton aphids with different bacterial types in cotton fields

AN Zhe¹^,²(), NIU Ruichang², ZHU Xiangzhen², WANG Li², ZHANG Kaixin², LI Dongyang², JI Jichao², NIU Lin², GAO Xueke², LUO Junyu², CUI Jinjie²(), MA Deying¹()

1. Key Laboratory of Monitoring and Safety Prevention and Control of Agriculture and Forest Pests, College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China
2. State Key Laboratory of Cotton Biology / Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang Henan 455000, China

Received:2024-02-20 Published:2024-09-20 Online:2024-10-09
Supported by:
Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(ZB2021046)

摘要/Abstract

摘要：

【目的】研究不同菌型棉蚜体内微生物种类与丰度的差异。【方法】通过HiSeq平台对不同菌型棉田棉蚜体内共生菌的16S rRNA基因V3~V4区进行高通量测序,分析绿盲蝽体内共生菌的种类与多样性。【结果】对照组与沙雷氏菌型、汉密尔顿菌型棉蚜的优势菌门均为变形菌门,相对物种丰度分别占97.42%、95.55%和92.78%。对照组与试验组的优势菌科均为肠杆菌科,但相对丰度有所差异,相对丰度分别占96.14%、81.285%和84.22%。汉密尔顿菌型棉蚜与沙雷氏菌型棉蚜其体内汉密尔顿菌属与沙雷氏菌属微生物丰度与对照组相比显著升高,分别占比77.40%和12.04%。【结论】棉田中含有沙雷氏菌与汉密尔顿菌的棉蚜其体内微生物丰度受到显著影响,其体内汉密尔顿菌属与沙雷氏菌属相对丰度显著上升。

关键词: 16S rRNA; 棉蚜; 微生物; 沙雷氏菌; 汉密尔顿菌

Abstract:

【Objective】 To explore the differences of microbial species and abundance in different types of Aphis gossypii Glove. 【Methods】 The V3-V4 region of 16SrRNA gene of symbiotic bacteria in different types of A. gossypii in cotton fields was sequenced by HiSeq platform, and the species and diversity of symbiotic bacteria in green bug bugs were analyzed. 【Results】 The dominant phylum of A. gossypii in the control group, serratia and Hamiltonella A. gossypii was Proteus, and the relative species abundance accounted for 97.42%, 95.55% and 92.78%, respectively. The dominant bacteria in the control group and the experimental group were Enterobacteriaceae, but the relative abundance was different, accounting for 96.14%, 81.285% and 84.22%, respectively. Compared with the control group, the microbial abundance of Hamiltonella and Serratia increased significantly, accounting for 77.40% and 12.04%, respectively. 【Conclusion】 The microbial abundance of A. gossypii containing Serratia and Hamiltonella in cotton field is significantly affected, and the relative abundance of Hamiltonella and Serratia increased significantly.

Key words: 16S rRNA; Aphis gossypii Glove; symbiotic bacteria; Hamiltonella spp.; Serratia spp.

中图分类号:

S435.62

安哲, 牛瑞昌, 朱香镇, 王丽, 张开心, 李东阳, 姬继超, 牛林, 高雪珂, 雒珺瑜, 崔金杰, 马德英. 棉田不同菌型棉蚜体内微生物多样性分析[J]. 新疆农业科学, 2024, 61(9): 2277-2284.

AN Zhe, NIU Ruichang, ZHU Xiangzhen, WANG Li, ZHANG Kaixin, LI Dongyang, JI Jichao, NIU Lin, GAO Xueke, LUO Junyu, CUI Jinjie, MA Deying. Analyze the microbial diversity of cotton aphids with different bacterial types in cotton fields[J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2277-2284.

图/表 7

图1 Obs指数稀释曲线注:A:对照组棉蚜种群;B:沙雷氏菌棉蚜种群;C:汉密尔顿菌棉蚜种群,下同

Fig.1 Obs exponent dilution curve Note:A:Control group Aphis gossypii populations;B:Serratia Aphis gossypii populations;C:Hamiltonella Aphis gossypii populations,the same as below

表1 各样本中的序列数(条)和Alpha多样性指数

Tab.1 Sequence number and Alpha diversity index for each sample

Samples	Number of reads	Number of OTUs	Chao	ACE	Shannon	Simpson	Coverage
A	377 680	59	163.925 40	205.339 59	0.588 4	0.777 78	0.999 12
B	395 108	56	177.230 86	209.957 57	1.047 65	0.644 03	0.999 15
C	428 742	14	175.649 41	192.584 21	0.928 44	0.622 17	0.999 1

图2 样本中的主要菌门水平

Fig.2 Main bacteria in samples

图3 样本中的主要菌科

Fig.3 Main bacteriaceae in samples

图4 样本中的主要菌属

Fig.4 Major genera in samples

图5 OTU韦恩图

Fig.5 Venn diagram of OTU

图6 物种PCA分析

Fig.6 PCA species analysis

参考文献 50

[1]	Crotti E, Balloi A, Hamdi C, et al. Microbial symbionts: a resource for the management of insect-related problems[J]. Microbial Biotechnology, 2012, 5(3): 307-317.
[2]	Engl T, Kaltenpoth M. Influence of microbial symbionts on insect pheromones[J]. Natural Product Reports, 2018, 35(5): 386-397.
[3]	Baumann P. Biology bacteriocyte-associated endosymbionts of plant sap-sucking insects[J]. Annual Review of Microbiology, 2005, (59): 155-189.
[4]	Gündüz E A, Douglas A E. Symbiotic bacteria enable insect to use a nutritionally inadequate diet[J]. ProceedingsBiological Sciences, 2009, 276(1658): 987-991.
[5]	Von Dohlen C D, Moran N A. Molecular data support a rapid radiation of aphids in the Cretaceous and multiple origins of host alternation[J]. Biological Journal of the Linnean Society, 2000, 71(4): 689-717.
[6]	Nakabachi A, Ishikawa H. Provision of riboflavin to the host aphid, Acyrthosiphonpisum, by endosymbiotic bacteria, Buchnera[J]. Journal of Insect Physiology, 1999, 45(1): 1-6.
[7]	Fukatsu T, Hosokawa T. Capsule-transmitted gut symbiotic bacterium of the Japanese common plataspid stinkbug, Megacoptapunctatissima[J]. Applied and Environmental Microbiology, 2002, 68(1): 389-396.
[8]	Chen D Q, Purcell A H. Occurrence and transmission of facultative endosymbionts in aphids[J]. Current Microbiology, 1997, 34(4): 220-225.
[9]	Tsuchida T, Koga R, Fujiwara A, et al. Phenotypic effect of “CandidatusRickettsiellaviridis,” a facultative symbiont of the pea aphid (Acyrthosiphonpisum), and its interaction with a coexisting symbiont[J]. Applied and Environmental Microbiology, 2014, 80(2): 525-533.
[10]	Heyworth E R, Ferrari J. A facultative endosymbiont in aphids can provide diverse ecological benefits[J]. Journal of Evolutionary Biology, 2015, 28(10): 1753-1760.
[11]	West S A, Cook J M, Werren J H, et al. Wolbachia in two insect host-parasitoid communities[J]. Molecular Ecology, 1998, 7(11): 1457-1465.
[12]	Fukatsu T, Tsuchida T, Nikoh N, et al. Spiroplasma symbiont of the pea aphid, Acyrthosiphonpisum (Insecta: Homoptera)[J]. Applied and Environmental Microbiology, 2001, 67(3): 1284-1291.
[13]	Jousselin E, Cœurd’Acier A, Vanlerberghe-Masutti F, et al. Evolution and diversity of Arsenophonus endosymbionts in aphids[J]. Molecular Ecology, 2013, 22(1): 260-270.
[14]	Chen D Q, Campbell B C, Purcell A H. A new rickettsia from a herbivorous insect, the pea aphid Acyrthosiphonpisum (Harris)[J]. Current Microbiology, 1996, 33(2): 123-128.
[15]	N., A., Moran, et al. Evolutionary Relationships of Three New Species of Enterobacteriaceae Living as Symbionts of Aphids and Other Insects[J]. Applied and Environmental Microbiology, 2005, 71(6): 3302-3310.
[16]	Montllor C B, Maxmen A, Purcell A H. Facultative bacterial endosymbionts benefit pea aphids Acyrthosiphonpisum under heat stress[J]. Ecological Entomology, 2002, 27(2): 189-195.
[17]	Slosser J E, Pinchak W E, Rummel D. A review of known and potential factors affecting the population dynamics of the cotton aphid[J]. Southwestern Entomologist, 1989.
[18]	Jacobson R J, Croft P. Strategies for the control of Aphis gossypii glover (Hom.: Aphididae) with Aphidiuscolemaniviereck (Hym.: Braconidae) in protected cucumbers[J]. Biocontrol Science and Technology, 1998, 8(3): 377-387.
[19]	Hullé M, Chaubet B, Turpeau E, et al. Encyclop’Aphid: a website on aphids and their natural enemies[J]. EntomologiaGeneralis, 2020, 40(1): 97-101.
[20]	Balabanidou V, Grigoraki L, Vontas J. Insect cuticle: a critical determinant of insecticide resistance[J]. Current Opinion in Insect Science, 2018, (27): 68-74.
[21]	Broderick N A, Raffa K F, Handelsman J. Midgut bacteria required for Bacillus thuringiensis insecticidal activity[J]. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(41): 15196-15199.
[22]	Kikuchi Y, Hayatsu M, Hosokawa T, et al. Symbiont-mediated insecticide resistance[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(22): 8618-8622.
[23]	Liu X D, Guo H F. Importance of endosymbionts Wolbachia and Rickettsia in insect resistance development[J]. Current Opinion in Insect Science, 2019, (33): 84-90.
[24]	Ghanim M, Kontsedalov S. Susceptibility to insecticides in the Q biotype of Bemisiatabaci is correlated with bacterial symbiont densities[J]. Pest Management Science, 2009, 65(9): 939-942.
[25]	Kontsedalov S, Zchori-Fein E, Chiel E, et al. The presence of Rickettsia is associated with increased susceptibility of Bemisiatabaci (Homoptera: Aleyrodidae) to insecticides[J]. Pest Management Science, 2008, 64(8): 789-792.
[26]	Zhang S, Su H H, Jiang W L, et al. Symbiotic microbial studies in diverse populations of Aphis gossypii, existing on altered host plants in different localities during different times[J]. Ecology and Evolution, 2021, 11(20): 13948-13960.
[27]	Oliver K M, Degnan P H, Burke G R, et al. Facultative symbionts in aphids and the horizontal transfer of ecologically important traits[J]. Annual Review of Entomology, 2010, (55): 247-266.
[28]	Meseguer A S, Manzano-Marín A, Coeur d’Acier A, et al. Buchnera has changed flatmate but the repeated replacement of co-obligate symbionts is not associated with the ecological expansions of their aphid hosts[J]. Molecular Ecology, 2017, 26(8): 2363-2378.
[29]	Oliver K M, Russell J A, Moran N A, et al. Facultative bacterial symbionts in aphids confer resistance to parasitic wasps[J]. Proceedings of the National Academy of Sciences of the United States of America, 2003, 100(4): 1803-1807.
[30]	Russell J A, Moran N A. Costs and benefits of symbiont infection in aphids: variation among symbionts and across temperatures[J]. ProceedingsBiological Sciences, 2006, 273(1586): 603-610.
[31]	Mago T, Salzberg S L. FLASH: fast length adjustment of short reads to improve genome assemblies[J]. Bioinformatics, 2011, 27(21): 2957-2963.
[32]	Edgar R C. UPARSE: highly accurate OTU sequences from microbial amplicon reads[J]. Nature Methods, 2013, 10(10): 996-998.
[33]	Edgar R C, Haas B J, Clemente J C, et al. UCHIME improves sensitivity and speed of chimera detection[J]. Bioinformatics, 2011, 27(16): 2194-2200.
[34]	Caporaso J G, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing data[J]. Nature Methods, 2010, (7): 335-336.
[35]	Edgar R C. Search and clustering orders of magnitude faster than BLAST[J]. Bioinformatics, 2010, 26(19): 2460-2461.
[36]	Schloss P D, Westcott S L, Ryabin T, et al. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities[J]. Applied and Environmental Microbiology, 2009, 75(23): 7537-7541.
[37]	Wilkinson T J, Huws S A, Edwards J E, et al. CowPI: a rumen microbiome focussed version of the PICRUSt functional inference software[J]. Frontiers in Microbiology, 2018, (9): 1095.
[38]	Chaves S, Neto M, Tenreiro R. Insect-symbiont systems: from complex relationships to biotechnological applications[J]. Biotechnology Journal, 2009, 4(12): 1753-1765.
[39]	Schloss P D, Delalibera I Jr, Handelsman J, et al. Bacteria associated with the guts of two wood-boring beetles: Anoplophoraglabripennis and Saperdavestita (Cerambycidae)[J]. Environmental Entomology, 2006, 35(3): 625-629.
[40]	Behar A, Yuval B, Jurkevitch E. Gut bacterial communities in the Mediterranean fruit fly (Ceratitis capitata) and their impact on host longevity[J]. Journal of Insect Physiology, 2008, 54(9): 1377-1383.
[41]	Zhang S, Luo J Y, Wang L, et al. Bacterial communities in natural versus pesticide-treated Aphis gossypii populations in North China[J]. MicrobiologyOpen, 2019, 8(3): 652-657.
[42]	Xu T T, Chen J, Jiang L Y, et al. Diversity of bacteria associated with Hormaphidinae aphids (Hemiptera: Aphididae)[J]. Insect Science, 2021, 28(1): 165-179.
[43]	Arneodo J D, Ortego J. Exploring the bacterial microbiota associated with native South American species ofAphis(Hemiptera: Aphididae)[J]. Environmental Entomology, 2014, 43(3): 589-594.
[44]	Gauthier J P, Outreman Y, Mieuzet L, et al. Bacterial communities associated with host-adapted populations of pea aphids revealed by deep sequencing of 16S ribosomal DNA[J]. PLoS One, 2015, 10(3): e0120664.
[45]	Tsuchida T, Koga R, Shibao H, et al. Diversity and geographic distribution of secondary endosymbiotic bacteria in natural populations of the pea aphid, Acyrthosiphonpisum[J]. Molecular Ecology, 2002, 11(10): 2123-2135.
[46]	Hansen A K, Moran N A. Aphid genome expression reveals host-symbiont cooperation in the production of amino acids[J]. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108(7): 2849-2854.
[47]	Koga R, Tsuchida T, Fukatsu T. Changing partners in an obligate symbiosis: a facultative endosymbiont can compensate for loss of the essential endosymbiont Buchnera in an aphid[J]. ProceedingsBiological Sciences, 2003, 270(1533): 2543-2550.
[48]	Oliver K M, Higashi C H. Variations on a protective theme: Hamiltonelladefensa infections in aphids variably impact parasitoid success[J]. Current Opinion in Insect Science, 2019, (32): 1-7.
[49]	Li Q, Sun J X, Qin Y G, et al. Reduced insecticide susceptibility of the wheat aphid Sitobion miscanthi after infection by the secondary bacterial symbiont Hamiltonelladefensa[J]. Pest Management Science, 2021, 77(4): 1936-1944.
[50]	Li Q, Fan J, Sun J X, et al. Anti-plant defense response strategies mediated by the secondary symbiont Hamiltonelladefensa in the wheat aphid Sitobion miscanthi[J]. Frontiers in Microbiology, 2019, (10): 2419.

棉田不同菌型棉蚜体内微生物多样性分析

Analyze the microbial diversity of cotton aphids with different bacterial types in cotton fields

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