基于农艺性状鹰嘴豆抗旱核心种质库构建

doi:10.6048/j.issn.1001-4330.2022.04.008

摘要/Abstract

摘要：

【目的】运用多年田间试验及种质遗传多样性分析筛选出100份抗旱性表现优异种质为参试材料,结合田间抗旱鉴定构建鹰嘴豆抗旱核心资源库,为鹰嘴豆抗旱种质利用提供基础材料。【方法】比较随机取样、位点优先取样、偏离度取样策略及取样比例,采用均值差异百分率、方差差异百分率、极差符合率以及变异系数变化率评价各核心子集农艺性状的变异保有量,筛选构建抗旱核心库。利用主成分分析确定鹰嘴豆抗旱种质核心库。【结果】采用的位点优先取样方法40%取样比例下构建的核心种质与原种质在多样性上无显著差异。并且检验种质各性状的均值与原种质符合率均大于96.33%,变异系数符合率大于97.25%,优于其它2种取样策略。主成分分析4个主成分包含各质量性状85%以上遗传多样性信息,确定构建的40份核心种质资源能够代表原种质资源遗传多样性。【结论】位点优先取样策略下,40%的取样比例所建立的鹰嘴豆抗旱核心种质资源库可以代表原种质资源的遗传多样性。

关键词: 鹰嘴豆; 抗旱性; 农艺性状; 种质资源; 核心库

Abstract:

【Objective】 Years of field trials and germplasm genetic diversity analysis screened out 100 germplasms with excellent drought resistance performance as test materials, combined with field drought resistance identification, the chickpea drought resistance core resource bank was initially constructed with a view to provide basic materials for drought resistant germplasm utilization of chickpea in the later stage.【Methods】 By comparing random sampling, site priority sampling, deviation sampling strategy and sampling ratio, the variation retention of agronomic traits in each core subset was evaluated by means of mean difference percentage, variance difference percentage, extreme difference coincidence rate and variation rate of coefficient of variation, and the final strategy of constructing drought-resistant core library was selected. In addition to that, the core database of drought resistant germplasm of chickpea was determined by principal component analysis.【Results】 There was no significant difference in diversity between the core collection constructed with the site-first sampling method at 40% sampling ratio and the original collection. And the coincidence rate of the mean value of each trait of the tested germplasm with the original germplasm was greater than 96.33%, and the coincidence rate of the coefficient of variation was greater than 97.25%, which was better than the other two sampling strategies.The four principal components of principal component analysis contained more than 85% genetic diversity information of each quality trait, and 40 core germplasm resources constructed could represent the genetic diversity of the original germplasm resources. 【Conclusion】 Under the site-priority sampling strategy, the chickpea drought-tolerant core germplasm bank established with a sampling ratio of 40% can represent the genetic diversity of the original germplasm resources.

Key words: chickpea; drought resistance; agronomic traits; germplasm resources; core germplasm bank

中图分类号:

S529

聂石辉, 王仙, 彭琳, 季良. 基于农艺性状鹰嘴豆抗旱核心种质库构建[J]. 新疆农业科学, 2022, 59(4): 847-854.

NIE Shihui, WANG Xian, PENG Lin, JI Liang. Preliminary Construction of Chickpea Drought Resistance Core Germplasm Based on Agronomic Traits[J]. Xinjiang Agricultural Sciences, 2022, 59(4): 847-854.

图/表 7

参考文献 24

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编号 No.	抗旱指数 DI	编号 No.	抗旱指数 DI	编号 No.	抗旱指数 DI	编号 No.	抗旱指数 DI	编号 No.	抗旱指数 DI
YZ-1	1.132	YZ-21	1.263	YZ-41	1.330	YZ-61	1.419	YZ-81	1.520
YZ-2	1.135	YZ-22	1.267	YZ-42	1.334	YZ-62	1.420	YZ-82	1.524
YZ-3	1.160	YZ-23	1.267	YZ-43	1.334	YZ-63	1.425	YZ-83	1.525
YZ-4	1.182	YZ-24	1.268	YZ-44	1.348	YZ-64	1.425	YZ-84	1.537
YZ-5	1.189	YZ-25	1.271	YZ-45	1.351	YZ-65	1.428	YZ-85	1.550
YZ-6	1.193	YZ-26	1.272	YZ-46	1.354	YZ-66	1.429	YZ-86	1.556
YZ-7	1.195	YZ-27	1.276	YZ-47	1.356	YZ-67	1.442	YZ-87	1.569
YZ-8	1.204	YZ-28	1.283	YZ-48	1.360	YZ-68	1.443	YZ-88	1.571
YZ-9	1.206	YZ-29	1.284	YZ-49	1.360	YZ-69	1.457	YZ-89	1.574
YZ-10	1.208	YZ-30	1.289	YZ-50	1.362	YZ-70	1.461	YZ-90	1.577
YZ-11	1.212	YZ-31	1.294	YZ-51	1.362	YZ-71	1.485	YZ-91	1.592
YZ-12	1.216	YZ-32	1.299	YZ-52	1.363	YZ-72	1.488	YZ-92	1.592
YZ-13	1.235	YZ-33	1.307	YZ-53	1.365	YZ-73	1.493	YZ-93	1.607
YZ-14	1.238	YZ-34	1.309	YZ-54	1.379	YZ-74	1.494	YZ-94	1.610
YZ-15	1.240	YZ-35	1.311	YZ-55	1.379	YZ-75	1.496	YZ-95	1.626
YZ-16	1.247	YZ-36	1.313	YZ-56	1.380	YZ-76	1.500	YZ-96	1.640
YZ-17	1.251	YZ-37	1.322	YZ-57	1.381	YZ-77	1.507	YZ-97	1.644
YZ-18	1.258	YZ-38	1.324	YZ-58	1.413	YZ-78	1.512	YZ-98	1.646
YZ-19	1.259	YZ-39	1.324	YZ-59	1.413	YZ-79	1.513	YZ-99	1.654
YZ-20	1.260	YZ-40	1.326	YZ-60	1.415	YZ-80	1.515	YZ-100	1.659

编号 No.	抗旱指数 DI	编号 No.	抗旱指数 DI	编号 No.	抗旱指数 DI	编号 No.	抗旱指数 DI	编号 No.	抗旱指数 DI
YZ-1	1.132	YZ-21	1.263	YZ-41	1.330	YZ-61	1.419	YZ-81	1.520
YZ-2	1.135	YZ-22	1.267	YZ-42	1.334	YZ-62	1.420	YZ-82	1.524
YZ-3	1.160	YZ-23	1.267	YZ-43	1.334	YZ-63	1.425	YZ-83	1.525
YZ-4	1.182	YZ-24	1.268	YZ-44	1.348	YZ-64	1.425	YZ-84	1.537
YZ-5	1.189	YZ-25	1.271	YZ-45	1.351	YZ-65	1.428	YZ-85	1.550
YZ-6	1.193	YZ-26	1.272	YZ-46	1.354	YZ-66	1.429	YZ-86	1.556
YZ-7	1.195	YZ-27	1.276	YZ-47	1.356	YZ-67	1.442	YZ-87	1.569
YZ-8	1.204	YZ-28	1.283	YZ-48	1.360	YZ-68	1.443	YZ-88	1.571
YZ-9	1.206	YZ-29	1.284	YZ-49	1.360	YZ-69	1.457	YZ-89	1.574
YZ-10	1.208	YZ-30	1.289	YZ-50	1.362	YZ-70	1.461	YZ-90	1.577
YZ-11	1.212	YZ-31	1.294	YZ-51	1.362	YZ-71	1.485	YZ-91	1.592
YZ-12	1.216	YZ-32	1.299	YZ-52	1.363	YZ-72	1.488	YZ-92	1.592
YZ-13	1.235	YZ-33	1.307	YZ-53	1.365	YZ-73	1.493	YZ-93	1.607
YZ-14	1.238	YZ-34	1.309	YZ-54	1.379	YZ-74	1.494	YZ-94	1.610
YZ-15	1.240	YZ-35	1.311	YZ-55	1.379	YZ-75	1.496	YZ-95	1.626
YZ-16	1.247	YZ-36	1.313	YZ-56	1.380	YZ-76	1.500	YZ-96	1.640
YZ-17	1.251	YZ-37	1.322	YZ-57	1.381	YZ-77	1.507	YZ-97	1.644
YZ-18	1.258	YZ-38	1.324	YZ-58	1.413	YZ-78	1.512	YZ-98	1.646
YZ-19	1.259	YZ-39	1.324	YZ-59	1.413	YZ-79	1.513	YZ-99	1.654
YZ-20	1.260	YZ-40	1.326	YZ-60	1.415	YZ-80	1.515	YZ-100	1.659

性状 Trait	平均值 Average	最小值 Min	最大值 Max	标准差 s	极差 Range	变异系数 CV/%
株高 Plant height ( cm )	45.61	28.73	76.13	14.558	47.40	16.32
单株一级分枝数 Number of prmary branches	5.66	3.22	9.80	1.698	6.58	18.41
单株荚数 Pods per palnt	138.64	22.26	296.45	76.911	274.19	48.80
每果节荚数 Pods per node	0.55	0.22	1.04	0.265	0.82	27.98
荚长 Pod length ( cm )	1.44	1.20	2.11	0.281	0.91	8.98
单株粒数 Seeds per plant	186.20	27.86	400.19	120.657	372.33	47.30
单株粒重 Seed yield per plant ( g )	36.89	8.43	76.38	21.763	67.95	40.50
百粒重 100-seed weight ( g )	19.74	6.67	38.71	10.290	32.04	23.21
产量 Yield per hectare ( kg/hm² )	2 039.45	1 126.65	3 561.73	56.695	2 435.08	12.23

性状 Trait	平均值 Average	最小值 Min	最大值 Max	标准差 s	极差 Range	变异系数 CV/%
株高 Plant height ( cm )	45.61	28.73	76.13	14.558	47.40	16.32
单株一级分枝数 Number of prmary branches	5.66	3.22	9.80	1.698	6.58	18.41
单株荚数 Pods per palnt	138.64	22.26	296.45	76.911	274.19	48.80
每果节荚数 Pods per node	0.55	0.22	1.04	0.265	0.82	27.98
荚长 Pod length ( cm )	1.44	1.20	2.11	0.281	0.91	8.98
单株粒数 Seeds per plant	186.20	27.86	400.19	120.657	372.33	47.30
单株粒重 Seed yield per plant ( g )	36.89	8.43	76.38	21.763	67.95	40.50
百粒重 100-seed weight ( g )	19.74	6.67	38.71	10.290	32.04	23.21
产量 Yield per hectare ( kg/hm² )	2 039.45	1 126.65	3 561.73	56.695	2 435.08	12.23

因子 Factor	特征值 Eigen value	贡献率 Average (%)	累计贡献率 Accumulating contribution rate(%)
1	4.232	40.590	40.590
2	2.733	24.214	64.804
3	1.194	12.467	77.271
4	1.040	10.221	87.492