蛋白质组学在番茄非生物逆境胁迫中的研究进展

doi:10.6048/j.issn.1001-4330.2021.10.008

摘要/Abstract

摘要：

【目的】回顾近年来蛋白质组学在番茄逆境胁迫中的研究进展,综述蛋白质组学技术在番茄响应非生物(盐碱、干旱、高温、低温、其它)胁迫上的研究进展,为利用蛋白质组学技术进一步研究番茄响应非生物逆境胁迫的分子机制奠定理论基础。【方法】运用统计学方法收集文献资料,并分析汇总蛋白质组学技术在番茄响应非生物(盐碱、干旱、高温、低温等)胁迫的研究文献进展情况。【结果】盐胁迫耐受性(渗透调节,渗透保护,离子稳态,消除氧清除剂,胁迫反应等)与胁迫的持续时间有关;下调的蛋白主要参与代谢和能量转换,上调的蛋白参与信号转导或运输;干旱应答蛋白包括与耐热性和渗透性保护剂的产生、脂质代谢、细胞壁修饰、神经酰胺代谢和丝裂原活化蛋白磷酸化相关的蛋白;蛋白质广泛参与了细胞过程,包括防御/应激反应,离子结合/转运,光合作用和蛋白质合成;最初如何感知胁迫条件,以及植物器官激活了哪些主要反应,可以避免低温胁迫晚期相关蛋白的干扰。【结论】在非生物逆境胁迫条件下,番茄通过改变自身的蛋白质表达水平对各种非生物胁迫作出响应。蛋白质组学研究能够全面揭示番茄响应胁迫时其细胞内蛋白质的动态变化规律,鉴定差异表达的蛋白质,是番茄抗逆生物学研究的重要组成部分。

关键词: 番茄; 非生物逆境胁迫; 蛋白质组

Abstract:

【Objective】 Abiotic stress is a key factor that restricts the growth and development of tomato, and affects the quality and yield of tomato crops, review in recent years, the research development of proteomics in tomato adversity stress, summarized proteomics technology in tomato response to abiotic (salt, drought, high temperature, low temperature, other) stress on the research progress of in-depth study for the use of proteomics technology.The molecular mechanism of tomato response to abiotic stress lays a theoretical foundation. 【Methods】 Statistical methods were used to collect literature data, and proteomics in tomato response to abiotic (saline-alkali, drought,High temperature, low temperature, etc.) stress was analyzed and summarized. 【Results】 Salt tolerance (osmotic regulation, osmotic protection, ion homeostasis, oxygen elimination scavengers, stress response, etc.) was related to the duration of stress. Down-regulated proteins were mainly involved in metabolism and energy conversion, and up-regulated proteins were involved in signal transduction or transport. Drought response proteins include proteins associated with heat and osmotic protectant production, lipid metabolism, cell wall modification, ceramide metabolism, and mitogen-activated protein phosphorylation. Proteins are involved in a wide range of cellular processes, including defense/stress responses, ion binding/transport, photosynthesis and protein synthesis, how stress conditions are initially perceived, and which major responses are activated by plant organs. 【Conclusion】 Under abiotic stress, tomato responded to various abiotic stresses by changing its protein expression level. Proteomic studies can reveal the dynamic changes of cellular proteins in tomato during stress response and identify differentially expressed proteins, which is an important part of tomato resistance biology research.

Key words: tomato; abiotic stress; proteome

中图分类号:

S641.2
S-1

王强, 王柏柯, 刘会芳, 韩宏伟, 庄红梅, 王娟, 杨涛, 王浩, 秦勇. 蛋白质组学在番茄非生物逆境胁迫中的研究进展[J]. 新疆农业科学, 2021, 58(10): 1829-1837.

Qiang WANG, Baike WANG, Huifang LIU, Hongwei HAN, Hongmei ZHUANG, Juan WANG, Tao YANG, Hao WANG, Yong QIN. Advances in Tomato Proteomics Research under Abiotic Stress[J]. Xinjiang Agricultural Sciences, 2021, 58(10): 1829-1837.

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