盐、碱及复合盐碱胁迫对番茄幼苗光合特性及抗氧化酶活性的影响

doi:10.6048/j.issn.1001-4330.2024.11.006

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (11): 2658-2666.DOI: 10.6048/j.issn.1001-4330.2024.11.006

• 种质资源·分子遗传学·生理生化·微生物 • 上一篇下一篇

盐、碱及复合盐碱胁迫对番茄幼苗光合特性及抗氧化酶活性的影响

刘会芳(), 王强(), 韩宏伟, 庄红梅, 王浩(), 常亚南

新疆农业科学院园艺作物研究所/新疆蔬菜工程技术研究中心/新疆蔬菜产业技术体系/新疆特色果蔬基因组研究与遗传改良重点实验室,乌鲁木齐 830091

收稿日期:2024-04-13 出版日期:2024-11-20 发布日期:2025-01-08
通信作者: 王强(1983-),男,甘肃人,研究员,研究方向为设施蔬菜栽培生理与逆境胁迫,(E-mail)wangqiang201004@sina.com；
王浩(1970-),男,山东人,研究员,研究方向为设施蔬菜栽培与生理,(E-mail)wanghao183@163.com
作者简介:刘会芳(1989-),女,河南人,助理研究员,硕士研究生,研究方向为蔬菜栽培和逆境生理,(E-mail)568899051@qq.com
基金资助:
新疆维吾尔自治区自然科学基金青年科学基金项目(2022D01B168);新疆农业科学院青年科技骨干创新能力培养项目(xjnkq-2023010);新疆维吾尔自治区重大科技专项(2022A02005-2);新疆蔬菜产业技术体系(XJARS-07-01);新疆蔬菜产业技术体系(XJARS-07-04);天山英才-科技创新领军人才(2023TSYCLJ0013)

Effects of salt, alkali and complex salt alkali stress on the photosynthetic characteristics and antioxidant enzyme activity of tomato seedlings

LIU Huifang(), WANG Qiang(), HAN Hongwei, ZHUANG Hongmei, WANG Hao(), CHANG Yanan

Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Xinjiang Vegetable Engineering Technology Research Center/XJARS-Vegetable/Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables,Urumqi 830091,China

Received:2024-04-13 Published:2024-11-20 Online:2025-01-08
Supported by:
Natural Science Foundation of Xinjiang Uygur Autonomous Region(2022D01B168);Youth Fund Project of Xinjiang Academy of Agricultural Sciences(xjnkq-2023010);Major Science and Technology Projects of Xinjiang(2022A02005-2);XJARS-Vegetable(XJARS-07-01);XJARS-Vegetable(XJARS-07-04);Tianshan Talents-Science and technology Innovation Leading Talent project(2023TSYCLJ0013)

摘要/Abstract

摘要：

【目的】研究盐、碱及复合盐碱胁迫对番茄幼苗光合特性及抗氧化酶活性的影响。【方法】以前期筛选的耐盐渐渗系番茄品种IL7-5-5及盐敏感栽培番茄品种M82为材料,通过对幼苗进行相同浓度的盐、碱及复合盐碱胁迫处理,研究不同胁迫条件下番茄幼苗光合特性、MDA、 $O_{2}^{-}$ 、H₂O₂含量以及SOD、POD、CAT、APX、GR活性的影响。【结果】与对照相比,盐胁迫条件下,番茄品种M82幼苗叶片光合气体交换参数显著降低,Pn降低了48.10%,WUE极显著增加了95.81%,POD和CAT活性极显著降低,GR活性显著增加,从而使 $O_{2}^{-}$ 和H₂O₂含量极显著降低了15.75%、25.85%;IL7-5-5番茄品种幼苗叶片Pn、Ci、WUE极显著降低,分别降低了34.72%、32.78%、51.02%,SOD活性极显著增加,GR活性显著增加,CAT和APX活性极显著降低,使MDA、 $O_{2}^{-}$ 和H₂O₂含量极显著降低了29.48%、34.47%和18.66%。碱胁迫条件下,番茄品种M82幼苗叶片光合气体交换参数Pn、Gs、Tr、CE极显著降低56.40%、71.02%、61.93%和41.73%,Ci显著降低了25.49%,POD和CAT活性极显著增加,APX活性极显著降低,使MDA含量极显著增加了40.55%, $O_{2}^{-}$ 和H₂O₂含量极显著降低了25.80%、20.48%;番茄品种IL7-5-5幼苗叶片光合气体交换参数Pn、Gs和Ci极显著降低68.75%、74.65%和59.49%,Tr、CE显著降低63.69%、21.54%,SOD活性极显著增加,CAT和GR活性极显著降低,使 $O_{2}^{-}$ 产生速率显著下降10.18%。盐碱胁迫条件下,番茄品种M82幼苗叶片光合气体交换参数Pn、Gs、Ci、Tr和CE极显著下降44.64%、67.05%、37.46%、56.56%和11.58%,POD活性极显著下降,APX活性显著下降,使 $O_{2}^{-}$ 极显著下降34.06%,MDA含量极显著增加37.44%;番茄品种IL7-5-5幼苗叶片光合气体交换参数Pn、Gs极显著下降27.78%、78.87%,Tr显著下降69.05%,WUE极显著增加128.17%,GR和SOD活性极显著增加,使 $O_{2}^{-}$ 产生速率极显著下降25.30%。【结论】2个番茄品种均能够通过调节抗氧化酶活性,达到清除 $O_{2}^{-}$ 和H₂O₂的效果,番茄品种IL7-5-5幼苗能够维持MDA含量在对照水平,比番茄品种M82膜脂过氧化程度轻。3种胁迫条件下,番茄幼苗的光合速率下降,2个番茄品种均以碱胁迫最低;碱胁迫和盐碱胁迫均对番茄品种M82幼苗造成了一定程度的膜脂过氧化损伤;胁迫不同程度的调节了2个番茄品种幼苗叶片抗氧化酶的活性、 $O_{2}^{-}$ 和H₂O₂的含量,其中2个番茄品种均以盐胁迫条件下H₂O₂的含量最低,其次是碱胁迫、盐碱胁迫。

关键词: 番茄; 盐胁迫; 碱胁迫; 复合盐碱胁迫; 光合特性; 酶活性

Abstract:

【Objective】 To explore the effects of salt, alkali and complex salt alkali stress on the photosynthetic characteristics and antioxidant enzyme activity of tomato seedlings.【Methods】 In this study, the salt-tolerant tomato IL7-5-5 and the salt-sensitive tomato M82 were selected as materials, and the seedlings were subjected to the same concentration of salt, alkali and complex salt alkali stress. The photosynthetic characteristics, contents of MDA, $O_{2}^{-}$ , H₂O₂ and activities of SOD, POD, CAT, APX and GR of tomato seedlings under different stress conditions were studied.【Results】 The results showed that: Compared with the control, under salt stress, photosynthetic gas exchange parameters of M82 tomato seedlings significantly decreased, Pn decreased by 48.10%, WUE increased by 95.81%, POD and CAT activities significantly decreased, and GR activity significantly increased. The contents of $O_{2}^{-}$ and H₂O₂ were significantly reduced by 15.75% and 25.85%. Leaf Pn, Ci and WUE of IL7-5-5 varieties were significantly decreased by 34.72%, 32.78% and 51.02%, respectively. SOD activity was significantly increased, GR activity was significantly increased, CAT and APX activities were significantly decreased. The contents of MDA, $O_{2}^{-}$ and H₂O₂ were significantly reduced by 29.48%, 34.47% and 18.66%. Under alkali stress, photosynthetic gas exchange parameters Pn, Gs, Tr and CE of M82 tomato seedlings significantly decreased by 56.40%, 71.02%, 61.93% and 41.73%, Ci significantly decreased by 25.49%, POD and CAT activities significantly increased, and APX activities significantly decreased. MDA content was significantly increased by 40.55%, while $O_{2}^{-}$ and H₂O₂ contents were significantly decreased by 25.80% and 20.48%. Photosynthetic gas exchange parameters Pn, Gs and Ci of IL7-5-5 tomato seedlings significantly decreased by 68.75%, 74.65% and 59.49%, Tr and CE significantly decreased by 63.69% and 21.54%, SOD activity significantly increased, CAT and GR activities significantly decreased. The production rate of $O_{2}^{-}$ decreased by 10.18%. Under salt-alkali stress, photosynthetic gas exchange parameters Pn, Gs, Ci, Tr and CE of M82 tomato seedlings significantly decreased by 44.64%, 67.05%, 37.46%, 56.56% and 11.58%, POD activity significantly decreased, APX activity significantly decreased, $O_{2}^{-}$ significantly decreased by 34.06%. MDA content was significantly increased by 37.44%; Photosynthetic gas exchange parameters Pn and Gs of IL7-5-5 tomato seedlings significantly decreased by 27.78% and 78.87%, Tr significantly decreased by 69.05%, WUE significantly increased by 128.17%, GR and SOD activity significantly increased, and $O_{2}^{-}$ production rate significantly decreased by 25.30%.【Conclusion】 Both tomato varieties can achieve the effect of eliminating $O_{2}^{-}$ and H₂O₂ by adjusting the activity of antioxidant enzymes. The content of MDA of IL7-5-5 tomato seedlings is maintained at the control level, and the degree of membrane lipid peroxidation is lighter than that of M82 tomato varieties. Under the three stress conditions, the photosynthetic rate of tomato seedlings decrease, and the alkali stress is the lowest in both varieties. Both alkali and saline-alkali stress causes membrane lipid peroxidation damage to M82 tomato seedlings to a certain extent. Stress can regulate the activity of antioxidant enzymes and the contents of $O_{2}^{-}$ and H₂O₂ in the leaves of the two tomato varieties to different degrees. The contents of H₂O₂ are the lowest in both tomato varieties under salt stress, followed by alkali stress and saline-alkali stress.

Key words: tomato; salt stress; alkali stress; compound saline-alkali stress; photosynthetic characteristics; enzyme activity

中图分类号:

S641.2

刘会芳, 王强, 韩宏伟, 庄红梅, 王浩, 常亚南. 盐、碱及复合盐碱胁迫对番茄幼苗光合特性及抗氧化酶活性的影响[J]. 新疆农业科学, 2024, 61(11): 2658-2666.

LIU Huifang, WANG Qiang, HAN Hongwei, ZHUANG Hongmei, WANG Hao, CHANG Yanan. Effects of salt, alkali and complex salt alkali stress on the photosynthetic characteristics and antioxidant enzyme activity of tomato seedlings[J]. Xinjiang Agricultural Sciences, 2024, 61(11): 2658-2666.

图/表 6

图1 盐、碱及复合盐碱胁迫下番茄幼苗叶片光合气体交换参数的变化注:小写字母表示处理间差异显著性(P<0.05),大写字母表示处理间差异显著性(P<0.01),下同

Fig.1 Changes of salt, alkali stress and combined salt-alkalistress on gas exchange parameters in leaves of tomato seedlings Notes: The lowercase letters mean significant difference ( P<0.05),The capital letters mean significant difference (P<0.01),the same as below

图2 盐、碱及复合盐碱胁迫下番茄幼苗WUE和CE的变化

Fig.2 Changes of salt, alkali stress and combined salt-alkalistress on WUE, CE in leaves of tomato seedlings

图3 盐、碱及复合盐碱胁迫下番茄幼苗叶片MDA含量的变化

Fig.3 Changes of salt, alkali stress and combined salt-alkalistress on gas exchange parameters in leaves of tomato seedlings

图4 盐、碱及复合盐碱胁迫下番茄幼苗叶片超氧阴离子产生速率及过氧化氢含量的变化

Fig.4 Changes of salt, alkali stress and combined salt-alkalistress on superoxide anion production rate and hydrogen peroxide content in leaves of tomato seedlings

图5 不同胁迫条件下番茄幼苗叶片SOD、POD、CAT活性的变化

Fig.5 Changes of different stress conditions on SOD, POD and CAT activities in leaves of tomato seedling

图6 不同胁迫条件下番茄幼苗叶片GR和APX活性的变化

Fig.6 Changes of different stress conditions on GR and APX activities in leaves of tomato seedlings

参考文献 15

[1]	闫永庆, 刘兴亮, 王崑, 等. 白刺对不同浓度混合盐碱胁迫的生理响应[J]. 植物生态学报, 2010, 34(10): 1213-1219. DOI
	YAN Yongqing, LIU Xingliang, WANG Kun, et al. Effect of complex saline-alkali stress on physiological parameters of Nitratia tangutorum[J]. Chinese Journal of Plant Ecology, 2010, 34(10): 1213-1219. DOI
[2]	刘建新, 王金成, 王瑞娟, 等. 混合盐碱胁迫对燕麦幼苗矿质离子吸收和光合特性的影响[J]. 干旱地区农业研究, 2017, 35(1): 178-184, 239.
	LIU Jianxin, WANG Jincheng, WANG Ruijuan, et al. Effect of complex saline-alkali stress on the mineral ions absorption and photosynthetic characteristics of oat seedlings[J]. Agricultural Research in the Arid Areas, 2017, 35(1): 178-184, 239.
[3]	王佺珍, 刘倩, 高娅妮, 等. 植物对盐碱胁迫的响应机制研究进展[J]. 生态学报, 2017, 37(16): 5565-5577.
	WANG Quanzhen, LIU Qian, GAO Yani, et al. Review on the mechanisms of the response to salinity-alkalinity stress in plants[J]. Acta Ecologica Sinica, 2017, 37(16): 5565-5577.
[4]	潘雄波, 向丽霞, 胡晓辉, 等. 外源亚精胺对盐碱胁迫下番茄幼苗根系线粒体功能的影响[J]. 应用生态学报, 2016, 27(2): 491-498.
	PAN Xiongbo, XIANG Lixia, HU Xiaohui, et al. Effects of exogenous spermidine on mitochondrial function of tomato seedling roots under salinity-alkalinity stress[J]. Chinese Journal of Applied Ecology, 2016, 27(2): 491-498. PMID
[5]	李辛, 赵文智. 荒漠区植物雾冰藜光合特性对混合盐碱胁迫的响应[J]. 生态学报, 2018, 38(4): 1183-1193.
	LI Xin,ZHAO Wenzhi. Response of various salt-alkaline mixed stresses on the photosynthetic characteristics of Bassia dasyphylla in a desert region[J]. Acta Ecologica Sinica, 2018, 38(4): 1183-1193.
[6]	刘会芳, 何晓玲, 肖春燕, 等. 外源GSH对NaCl胁迫下番茄幼苗光合特性及碳同化关键酶基因表达的影响[J]. 应用生态学报, 2014, 25(9): 2637-2644.
	LIU Huifang, HE Xiaoling, XIAO Chunyan, et al. Effects of exogenous GSH on photosynthetic characteristics and expression of key enzyme genes of CO₂ assimilation in leaves of tomato seedlings under NaCl stress[J]. Chinese Journal of Applied Ecology, 2014, 25(9): 2637-2644.
[7]	Sreenivasulu N, Grimm B, Wobus U, et al. Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedlings of foxtail millet (Setaria italica)[J]. Physiologia Plantarum, 2000, 109(4): 435-442.
[8]	Geng G, Li R R, Stevanato P, et al. Physiological and transcriptome analysis of sugar beet reveals different mechanisms of response to neutral salt and alkaline salt stresses[J]. Frontiers in Plant Science, 2020, 11: 571864.
[9]	Lin J X, Yu D F, Shi Y J, et al. Salt-alkali tolerance during germination and establishment of Leymus chinensis in the Songnen Grassland of China[J]. Ecological Engineering, 2016, 95: 763-769.
[10]	Song T T, Xu H H, Sun N, et al. Metabolomic analysis of alfalfa (Medicago sativa L.) root-symbiotic rhizobia responses under alkali stress[J]. Frontiers in Plant Science, 2017, 8: 1208.
[11]	Shi C C, Yang F, Liu Z H, et al. Uniform water potential induced by salt, alkali, and drought stresses has different impacts on the seedling of Hordeum jubatum: from growth, photosynthesis, and chlorophyll fluorescence[J]. Frontiers in Plant Science, 2021, 12: 733236.
[12]	郭瑞, 周际, 杨帆, 等. 碱胁迫对小麦(Triticum aestivum linn)叶片代谢过程的影响[J]. 中国农业科学, 2017, 50(2): 250-259. DOI
	GUO Rui, ZHOU Ji, YANG Fan, et al. Effects of alkaline stress on metabonomic responses of wheat(Triticum aestivum linn) leaves[J]. Scientia Agricultura Sinica, 2017, 50(2): 250-259. DOI
[13]	石德成, 赵可夫. NaCl和Na2CO3对星星草生长及营养液中主要矿质元素存在状态的影响[J]. 草业学报, 1997, 6(2): 51-61.
	SHI Decheng, ZHAO Kefu. Effects of NaCl and Na₂CO₃ on the growth of PUCCINELLIA tenuiflora and the existing state of main mineral elements in nutrient solution[J]. Acta Prataculturae Sinica, 1997, 6(2): 51-61.
[14]	Wang C, Zhang S H, Wang P F, et al. Metabolic adaptations to ammonia-induced oxidative stress in leaves of the submerged macrophyte Vallisneria natans (Lour.) Hara[J]. Aquatic Toxicology, 2008, 87(2): 88-98. DOI PMID
[15]	邹晓云, 向华, 于晓英. Cu²⁺胁迫对香菇草生长和生理生化特性的影响[J]. 天津农业科学, 2011, 17(1): 22-24.
	ZOU Xiaoyun, XIANG Hua, YU Xiaoying. Effects of Cu²⁺ stress on the growth and physiological and biochemical characteristics of Hydrocotyle vulgaris[J]. Tianjin Agricultural Sciences, 2011, 17(1): 22-24.

盐、碱及复合盐碱胁迫对番茄幼苗光合特性及抗氧化酶活性的影响

Effects of salt, alkali and complex salt alkali stress on the photosynthetic characteristics and antioxidant enzyme activity of tomato seedlings

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