新疆农业科学 ›› 2024, Vol. 61 ›› Issue (7): 1727-1737.DOI: 10.6048/j.issn.1001-4330.2024.07.020
刘会芳(), 韩宏伟, 庄红梅, 王强(
), 高阿香, 王浩(
)
收稿日期:
2024-01-28
出版日期:
2024-07-20
发布日期:
2024-09-04
通信作者:
王强(1983-),男,甘肃人,研究员,博士,硕士生导师,研究方向为设施蔬菜栽培生理与逆境胁迫,(E-mail)wangqiang201004@sina.com;作者简介:
刘会芳(1989-),女,河南人,助理研究员,硕士研究生,研究方向为蔬菜栽培和逆境生理,(E-mail)568899051@qq.com
基金资助:
LIU Huifang(), HAN Hongwei, ZHUANG Hongmei, WANG Qiang(
), GAO Axiang, WANG Hao(
)
Received:
2024-01-28
Published:
2024-07-20
Online:
2024-09-04
Supported by:
摘要:
【目的】研究增施微生物肥对土壤微生物群落结构和功能的影响,为设施蔬菜栽培过程中微生物肥的使用、土壤改良及提高蔬菜产量提供理论基础。【方法】选择蔬菜为黄瓜,以土壤微生物为研究对象,以不施用微生物肥土壤为对照,采用16S rRNA和真菌ITS区测序,分析微生物肥对土壤微生物群落结构和功能的影响。【结果】增施微生物肥可增加拟杆菌门、绿弯菌门和厚壁菌门的细菌丰度和担子菌门的真菌丰度,降低子囊菌门和被孢霉门的真菌丰度;增加土壤中微生物的均匀度和细菌的多样性,降低真菌多样性;通过增加有益细菌的含量、减少病源真菌、丰富种群,有效改善土壤微生物群落功能,微生物群落的代谢功能强于对照土壤。【结论】施用微生物肥料可以改善土壤营养环境,为土壤微生物的生命活动提供充足的养分和能源,从而促进微生物生长繁殖,丰富种群,有效改善土壤微生物群落功能。
中图分类号:
刘会芳, 韩宏伟, 庄红梅, 王强, 高阿香, 王浩. 增施微生物肥对设施黄瓜土壤微生物多样性的影响[J]. 新疆农业科学, 2024, 61(7): 1727-1737.
LIU Huifang, HAN Hongwei, ZHUANG Hongmei, WANG Qiang, GAO Axiang, WANG Hao. Effects of increased application of microbial fertilizer on soil microbial diversity of cucumber in facilities[J]. Xinjiang Agricultural Sciences, 2024, 61(7): 1727-1737.
图1 不同样品土壤细菌门水平(左)和真菌门水平(右)相对丰度
Fig.1 The Relative abundance of bacteria at phylum level (left) and fungi at phylum level (right) in the different samples
图2 不同样品土壤细菌类属水平(上)和真菌类属水平(下)相对丰度
Fig.2 The Relative abundance of bacteria at genus level (up) and fungi at genus level (down) in the different soil samples
项目 Items | 处理 Treatments | 超1指数 Chao1 | 覆盖率 Ccoverage(%) | 皮耶罗均匀度 Pielou evenness | 香浓指数 Shannon index | 辛普森多样性指数 Simpson index |
---|---|---|---|---|---|---|
细菌 | H0 | 3 120.763 3 | 99.13 | 0.804 6 | 9.272 3 | 0.964 5 |
Bacteria | H1 | 3 146.606 7 | 99.15 | 0.852 5 | 9.861 7 | 0.990 0 |
真菌 | H0 | 337.959 3 | 99.997 | 0.573 8 | 4.817 3 | 0.868 6 |
Fungi | H1 | 347.045 0 | 99.997 | 0.487 2 | 4.111 6 | 0.818 6 |
表1 不同样品土壤细菌、真菌α多样性指数的变化
Tab.1 Changes of α diversity index statistics of fungi and bacteria in different soil samples
项目 Items | 处理 Treatments | 超1指数 Chao1 | 覆盖率 Ccoverage(%) | 皮耶罗均匀度 Pielou evenness | 香浓指数 Shannon index | 辛普森多样性指数 Simpson index |
---|---|---|---|---|---|---|
细菌 | H0 | 3 120.763 3 | 99.13 | 0.804 6 | 9.272 3 | 0.964 5 |
Bacteria | H1 | 3 146.606 7 | 99.15 | 0.852 5 | 9.861 7 | 0.990 0 |
真菌 | H0 | 337.959 3 | 99.997 | 0.573 8 | 4.817 3 | 0.868 6 |
Fungi | H1 | 347.045 0 | 99.997 | 0.487 2 | 4.111 6 | 0.818 6 |
[1] | Janvier C, Villeneuve F, Alabouvette C, et al. Soil health through soil disease suppression: Which strategy from descriptors to indicators?[J]. Soil Biology and Biochemistry, 2007, 39(1): 1-23. |
[2] | Salles J F, van Veen J A, van Elsas J D. Multivariate analyses of Burkholderia species in soil: effect of crop and land use history[J]. Applied and Environmental Microbiology, 2004, 70(7): 4012-4020. |
[3] | Enwall K, Nyberg K, Bertilsson S, et al. Long-term impact of fertilization on activity and composition of bacterial communities and metabolic guilds in agricultural soil[J]. Soil Biology and Biochemistry, 2007, 39(1): 106-115. |
[4] | Cardinale B J, Srivastava D S, Emmett Duffy J, et al. Effects of biodiversity on the functioning of trophic groups and ecosystems[J]. Nature, 2006, 443(7114): 989-992. |
[5] | 刘海, 王玉书, 焦玉洁, 等. 三种土壤条件下紫茎泽兰根际的酶活性及细菌群落状况[J]. 生态学报, 2018, 38(23): 8455-8465. |
LIU Hai, WANG Yushu, JIAO Yujie, et al. Enzyme activities and bacterial community in the rhizosphere of Eupatorium adenophorum under different soil conditions[J]. Acta Ecologica Sinica, 2018, 38(23): 8455-8465. | |
[6] | 李荣, 宋维峰. 哈尼梯田生态系统土壤微生物量碳的影响因素[J]. 生态学报, 2020, 40(17): 6223-6232. |
LI Rong, SONG Weifeng. Factors affecting soil microbial biomass carbon in Hani terraced ecosystem[J]. Acta Ecologica Sinica, 2020, 40(17): 6223-6232. | |
[7] | Zelles L, Bai Q Y, Beck T, et al. Signature fatty acids in phospholipids and lipopolysaccharides as indicators of microbial biomass and community structure in agricultural soils[J]. Soil Biology and Biochemistry, 1992, 24(4): 317-323. |
[8] | Larkin R P, Honeycutt C W. Effects of different 3-year cropping systems on soil microbial communities and Rhizoctonia diseases of potato[J]. Phytopathology©, 2006, 96(1): 68-79. |
[9] |
Yao H, He Z, Wilson M, et al. Microbial biomass and community structure in a sequence of soils with increasing fertility and changing land use[J]. Microbial Ecology, 2000, 40(3): 223-237.
PMID |
[10] | 孙昌禹, 贾永国, 王淑芬. 氮肥施用对生态系统的影响及措施的研究[J]. 河北农业科学, 2009, 13(3): 60-63. |
SUN Changyu, JIA Yongguo, WANG Shufen. Effects of nitrogen fertilization on ecosystem and countermeasures of improving the nitrogen use efficiency[J]. Journal of Hebei Agricultural Sciences, 2009, 13(3): 60-63. | |
[11] | 郝晶, 刘冰, 谢英荷, 等. 不同氮素水平下生物菌肥施用效果研究[J]. 山西农业科学, 2006, 34(1): 50-52. |
HAO Jing, LIU Bing, XIE Yinghe, et al. Application effect of biological fertilizer in different-level nitrogen[J]. Journal of Shanxi Agricultural Sciences, 2006, 34(1): 50-52. | |
[12] | 谢达平, 雷女孝, 彭道林, 等. 微生物菌肥的作用机理研究[J]. 常德师范学院学报(自然科学版), 2002, 14(1): 48-50, 67. |
XIE Daping, LEI Xiao, PENG Daolin, et al. The study of the bacteria manure’s role to the crop[J]. Journal of Changde Teachers University, 2002, 14(1): 48-50, 67. | |
[13] | 王素英, 陶光灿, 谢光辉, 等. 我国微生物肥料的应用研究进展[J]. 中国农业大学学报, 2003, 8(1): 14-18. |
WANG Suying, TAO Guangcan, XIE Guanghui, et al. A review of effects of biofertilizers on crop yield and quality[J]. Journal of China Agricultural University, 2003, 8(1): 14-18. | |
[14] | Diosma G, Aulicino M, Chidichimo H, et al. Effect of tillage and N fertilization on microbial physiological profile of soils cultivated with wheat[J]. Soil and Tillage Research, 2006, 91(1/2): 236-243. |
[15] | Yuan J, Wen T, Zhang H, et al. Predicting disease occurrence with high accuracy based on soil macroecological patterns of Fusarium wilt[J]. The ISME Journal, 2020, 14(12): 2936-2950. |
[16] | 王涛, 辛世杰, 乔卫花, 等. 几种微生物菌肥对连作黄瓜生长及土壤理化性状的影响[J]. 中国蔬菜, 2011,(18): 52-57. |
WANG Tao, XIN Shijie, QIAO Weihua, et al. Effects of different microbial fertilizers on continuous cropping cucumber growth and soil physiochemical properties[J]. China Vegetables, 2011,(18): 52-57. | |
[17] | Wan T T, Zhao H H, Wang W. Effect of biocontrol agent Bacillus amyloliquefaciens SN16-1 and plant pathogen Fusarium oxysporum on tomato rhizosphere bacterial community composition[J]. Biological Control, 2017, (112): 1-9. |
[18] | 白洋, 钱景美, 周俭民, 等. 农作物微生物组: 跨越转化临界点的现代生物技术[J]. 中国科学院院刊, 2017, 32(3): 260-265. |
BAI Yang, QIAN Jingmei, ZHOU Jianmin, et al. Crop microbiome: breakthrough technology for agriculture[J]. Bulletin of Chinese Academy of Sciences, 2017, 32(3): 260-265. | |
[19] | 黎妍妍, 王林, 彭五星, 等. 解淀粉芽孢杆菌施用方式对烟株根际土壤真菌群落的影响[J]. 中国烟草科学, 2020, 41(4): 1-6. |
LI Yanyan, WANG Lin, PENG Wuxing, et al. Promotion effects of three application methods of Bacillus amyloliquefaciens on fungal community in tobacco rhizosphere soil[J]. Chinese Tobacco Science, 2020, 41(4): 1-6. | |
[20] | Han L J, Wang Z Y, Li N, et al. Bacillus amyloliquefaciens B1408 suppresses Fusarium wilt in cucumber by regulating the rhizosphere microbial community[J]. Applied Soil Ecology, 2019, (136): 55-66. |
[21] | 张云霞, 雷鹏, 许宗奇, 等. 一株高效解磷菌Bacillus subtilis JT-1的筛选及其对土壤微生态和小麦生长的影响[J]. 江苏农业学报, 2016, 32(5): 1073-1080. |
ZHANG Yunxia, LEI Peng, XU Zongqi, et al. Screening of a high-efficiency phosphate solubilizing bacterium Bacillus subtilis JT-1 and its effects on soil microecology and wheat growth[J]. Jiangsu Journal of Agricultural Sciences, 2016, 32(5): 1073-1080. | |
[22] | Zhao J, Wang Y G, Liang H, et al. The rhizosphere microbial community response to a bio-organic fertilizer: finding the mechanisms behind the suppression of watermelon Fusarium wilt disease[J]. Acta Physiologiae Plantarum, 2017, 40(1): 17. |
[23] | 陶晶, 李晖, 李春. 芽胞杆菌组合BCL-8对番茄的促生防病效果及其促生机制初探[J]. 植物保护, 2010, 36(1): 87-91. |
TAO Jing, LI Hui, LI Chun. Growth-promotion and disease control effect of combination BCL-8 on tomato and growth-promotion mechanism[J]. Plant Protection, 2010, 36(1): 87-91. | |
[24] | 胡青平, 徐建国, 程丽萍, 等. 枯草芽胞杆菌QM3防治番茄早疫病和促进幼苗生长的研究[J]. 植物保护, 2011, 37(4): 95-100. |
HU Qingping, XU Jianguo, CHENG Liping, et al. Studies on promoting tomato growth and controlling early blight using Bacillus subtilis QM3[J]. Plant Protection, 2011, 37(4): 95-100. | |
[25] |
Islam M R, Sultana T, Joe M M, et al. Nitrogen-fixing bacteria with multiple plant growth-promoting activities enhance growth of tomato and red pepper[J]. Journal of Basic Microbiology, 2013, 53(12): 1004-1015.
DOI PMID |
[1] | 曾婉盈, 耿洪伟, 程宇坤, 李思忠, 钱松廷, 高卫时, 张立明. 甜菜品系叶丛快速生长期抗旱性综合评价[J]. 新疆农业科学, 2024, 61(9): 2140-2151. |
[2] | 徐毛毛, 高杰, 李君明, 李鑫, 刘磊, 潘峰. 20个番茄商业品种群体的多样性分析[J]. 新疆农业科学, 2024, 61(9): 2191-2196. |
[3] | 苗雨, 陈翠霞, 马艳明, 邢国芳, 董裕生, 陈智军, 王仙, 向莉. 276份中亚大麦种质资源表型性状的遗传多样性分析[J]. 新疆农业科学, 2024, 61(8): 1888-1895. |
[4] | 王庆朋, 闫成才, 王喆, 苟长青, 王兰, 冯宏祖, 郝海婷. 库尔勒香梨开花前后花药细菌多样性分析[J]. 新疆农业科学, 2024, 61(8): 1976-1982. |
[5] | 杨璐, 王娜, 范少丽, 程平, 李宏, 汪阳东. 黑桑种质资源表型性状变异特征分析[J]. 新疆农业科学, 2024, 61(5): 1172-1181. |
[6] | 杨跃发, 王春霞, 梁飞, 蓝明菊. 季节性冻融期土壤微生物的数量影响因素Meta分析[J]. 新疆农业科学, 2024, 61(5): 1236-1249. |
[7] | 耿美菊, 王新绘, 刘晓颖, 吕佩. 封育对荒漠草原真菌群落的影响[J]. 新疆农业科学, 2024, 61(5): 1250-1258. |
[8] | 姚宇翔, 王国强, 王可, 伊莎, 杨欣雅, 罗晓霞. 塔里木河上游不同生境细菌群落结构及多样性分析[J]. 新疆农业科学, 2024, 61(3): 708-718. |
[9] | 蒲明, 宋学栋, 张鹏, 张明敏, 张代玉, 薛莲, 袁宁, 王海鹏, 高铁功. 不同滴灌技术水肥耦合在设施黄瓜上的应用分析[J]. 新疆农业科学, 2024, 61(2): 402-412. |
[10] | 王帆, 李玉姗, 王威, 邓超宏, 赵连佳, 马越, 肖菁, 庄红梅, 许红军. 基于表型性状鉴定及SSR分子标记分析芜菁种质资源遗传多样性[J]. 新疆农业科学, 2024, 61(11): 2601-2613. |
[11] | 李超, 杨英, 郑贺云, 杨建丽, 陈伟, 杨咪, 孙玉萍. 基于SSR荧光标记分析新疆甜瓜种质资源遗传多样性与群体结构[J]. 新疆农业科学, 2024, 61(11): 2614-2625. |
[12] | 马钥珺, 谭占明, 程云霞, 吴慧, 张乔乔, 杜佳庚, 王琦, 崔贺伟, 马兴. 不同基质配比及砧穗组合对黄瓜生长发育的影响[J]. 新疆农业科学, 2024, 61(11): 2635-2647. |
[13] | 黄幸磊, 王为然, 王萌, 朱家辉, 林峰, 秦国礼, 杨静, 阿里甫·艾尔西, 吴全忠, 孔杰. 海岛棉种质资源机采农艺性状相关性分析[J]. 新疆农业科学, 2024, 61(1): 1-8. |
[14] | 于吕健, 阳瑾, 丁宇, 李晓曼, 刘峰娟, 范盈盈, 梁红玉, 焦子伟, 王成. 石榴果实表面真菌的分离鉴定及致病性测定[J]. 新疆农业科学, 2024, 61(1): 148-155. |
[15] | 席欧彦, 王晨日, 古丽奴尔·吐拉西, 胡红英. 伊犁河谷野果林传粉昆虫物种多样性及访花行为特征[J]. 新疆农业科学, 2024, 61(1): 190-198. |
阅读次数 | ||||||||||||||||||||||||||||||||||||||||||||||||||
全文 37
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||
摘要 103
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||