玉米ZmCDPK22基因在干旱胁迫下的表达分析

doi:10.6048/j.issn.1001-4330.2023.06.009

摘要/Abstract

摘要：

【目的】研究玉米ZmCDPK22基因的功能特征,分析该基因的编码序列,分析物种间的进化关系及蛋白互作及其功能,以叶片含水量作为干旱胁迫指标,研究ZmCDPK22在干旱胁迫下的表达特性。【方法】利用生物信息学手段分析该基因的基本特征,运用实时荧光定量(Quantitative Real-time PCR,qRT-PCR)技术分析该基因在干旱胁迫下的表达特性。【结果】该基因编码一个分子式为C₂₆₇₅H₄₂₁₇N₇₇₉O₇₉₈S₂₁,分子量为60 731.91,氨基酸大小为539 aa,等电点为6.44的亲水性蛋白。该蛋白无跨膜结构以及信号肽,预测结果显示该蛋白定位于细胞核且与10个蛋白存在互作,属于PK_like亚家族;该基因启动区含有大量的光响应和多种激素响应等元件。在不同含水量情况下,ZmCDPK22的表达量不同,与叶片含水量87%相比,其相对表达量随着叶片含水量的降低而逐渐降低,在叶片含水量为78%时相对表达量最高,在含水量为33%时相对表达量最低。【结论】该基因在叶片不同含水量的表达量不同,该基因响应干旱胁迫,可能在干旱应答反应中发挥一定功能。

关键词: 玉米; ZmCDPK22; 干旱胁迫; 生物信息学; 基因表达

Abstract:

【Objective】 In order to explore the functional characteristics of ZmCDPK22 gene in maize. We predict the function of ZmCDPK22 theoretically through analysing evolutionary relationships among species and protein interactions. Taking water content of leaf as an index of drought stress to determine the expression characteristics of ZmCDPK22 under drought stress. 【Methods】 The basic characteristics of ZmCDPK22 and its relative expression patterns in response to drought stress were analyzed by bioinformatics and RT-qPCR. 【Results】 The ZmCDPK22 protein had a molecular weight of 60,731.91 and a theoretical isoelectric point of 6.44 and which encoding a hydrophilic protein with molecular formula C₂₆₇₅H₄₂₁₇N₇₇₉O₇₉₈S₂₁and 539 amino acids. The protein was mainly localized in the nucleus and had no signal peptide and transmembrane structure. Protein-protein interaction prediction result showed that ZmCDPK22 could interact with ten proteins, and it belongs to the PK_like subfamily. The promoter region of ZmCDPK22 containd a large number of Cis-acting elements such as light response and hormone response. qRT-PCR results revealed that the expression of ZmCDPK22 was different under different water content. Compared with 87%water content of leaf, the relative expression of ZmcDPK22 decreased gradually with the decrease of water content of leavf. The relative expression of ZmcDPK22 was the highest when the water content was 78%, and the lowest when the water content was 33%. 【Conclusion】 The expression of this gene was different in different water content of leaves, indicating that this gene was responsive to drought stress and might play a role in drought response.

Key words: maize; ZmCDPK22; drought stress; bioinformation; gene expression

中图分类号:

S513

邵盘霞, 赵准, 邵武奎, 郝晓燕, 高升旗, 李建平, 胡文冉, 黄全生. 玉米ZmCDPK22基因在干旱胁迫下的表达分析[J]. 新疆农业科学, 2023, 60(6): 1372-1378.

SHAO Panxia, ZHAO Zhun, SHAO Wukui, HAO Xiaoyan, GAO Shengqi, LI Jianping, HU Wenran, HUANG Quansheng. Expression analysis of ZmCDPK22 gene in maize under drought stress[J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1372-1378.

图/表 7

参考文献 28

[1]	Kuromori T, Fujita M, Takahashi F, et al. Inter‐tissue and inter‐organ signaling in drought stress response and phenotyping of drought tolerance[J]. The Plant Journal, 2022, 109(2):342-358. DOI URL
[2]	Chen H, Dong J, Wang T. Autophagy in Plant Abiotic Stress Management[J]. International Journal of Molecular Sciences, 2021, 22(8):4075. DOI URL
[3]	Kudla J, Batistiĉ O, Hashimoto K. Calcium Signals: The Lead Currency of Plant Information Processing[J]. The Plant Cell, 2012, 22(3):541-563. DOI URL
[4]	Valmonte G R, Arthur K, Higgins C M, et al. Calcium-Dependent Protein Kinases in Plants: Evolution, Expression and Function[J]. Plant and Cell Physiology, 2014, 55(3):551-569. DOI PMID
[5]	Zhu S, Yu X, Wang X, et al. Two Calcium-Dependent Protein Kinases, CPK4 and CPK11, Regulate Abscisic Acid Signal Transduction in Arabidopsis[J]. The Plant Cell, 2007, 19(10):3019-3036. DOI URL
[6]	Zou J, Li X, Ratnasekera D, et al. Arabidopsis CALCIUM-DEPENDENT PROTEIN KINASE8 and CATALASE3 Function in Abscisic Acid-Mediated Signaling and H2O2 Homeostasis in Stomatal Guard Cells under Drought Stress[J]. The Plant Cell, 2015, 27(5):1445-1460. DOI URL
[7]	Zou J, Wei F, Wang C, et al. Arabidopsis Calcium-Dependent Protein Kinase CPK10 Functions in Abscisic Acid- and Ca²⁺-Mediated Stomatal Regulation in Response to Drought Stress[J]. Plant Physiology, 2010, 154(3):1232-1243. DOI URL
[8]	Campo S, Baldrich P, Messeguer J, et al. Overexpression of a Calcium-Dependent Protein Kinase Confers Salt and Drought Tolerance in Rice by Preventing Membrane Lipid Peroxidation?[J]. Plant Physiology, 2014, 165(2):688-704. PMID
[9]	Wei S, Hu W, Deng X, et al. A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility[J]. BMC Plant Biol, 2014, 14:133. DOI PMID
[10]	Vivek P J, Tuteja N, Soniya E V. CDPK1 from ginger promotes salinity and drought stress tolerance without yield penalty by improving growth and photosynthesis in Nicotiana tabacum[J]. PLoS One, 2013, 8(10):e76392. DOI URL
[11]	Kong X, Lv W, Jiang S, et al. Genome-wide identification and expression analysis of calcium-dependent protein kinase in maize[J]. BMC Genomics, 2013, 14(1):433. DOI
[12]	Weckwerth P, Ehlert B, Romeis T. ZmCPK1, a calcium-independent kinase member of the Zea mays CDPK gene family, functions as a negative regulator in cold stress signalling[J]. Plant, Cell & Environment, 2015, 38(3):544-558.
[13]	Szczegielniak J, Borkiewicz L, Szurmak B, et al. Maize calcium-dependent protein kinase (ZmCPK11): local and systemic response to wounding, regulation by touch and components of jasmonate signalling[J]. Physiologia Plantarum, 2012, 146(1):1-14. DOI URL
[14]	Li C, Wang M, Wu X, et al. THI1, a Thiamine Thiazole Synthase, Interacts with Ca²⁺-Dependent Protein Kinase CPK33 and Modulates the S-Type Anion Channels and Stomatal Closure in Arabidopsis?[J]. Plant Physiology, 2016, 170(2):1090-1104. DOI URL
[15]	Huang K, Peng L, Liu Y, et al. Arabidopsis calcium-dependent protein kinase AtCPK1 plays a positive role in salt/drought-stress response[J]. Biochemical and Biophysical Research Communications, 2018, 498(1):92-98. DOI PMID
[16]	Zhang H, Liu D, Yang B, et al. Arabidopsis CPK6 positively regulates ABA signaling and drought tolerance through phosphorylating ABA-responsive element-binding factors[J]. Journal of Experimental Botany, 2020, 71(1):188-203. DOI URL
[17]	Zhao P, Liu Y, Kong W, et al. Genome-Wide Identification and Characterization of Calcium-Dependent Protein Kinase (CDPK) and CDPK-Related Kinase (CRK) Gene Families in Medicago truncatula[J]. International Journal of Molecular Sciences, 2021, 22(3):1044. DOI URL
[18]	赵长江, 彭晶晶, 董洁静, 等. 玉米rboh基因家族鉴定及非生物逆境表达分析[J]. 玉米科学, 2021, 29(4):43-50.
	ZHAO Changjiang, PENG Jingjing, DONG Jiejing, et al. Analysis of abiotic stress expression of rboh gene family identified in maize genome[J]. Journal of Maize Sciences, 2021, 29(4):43-50.
[19]	Cheng C, Xu X, Gao M, et al. Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.)[J]. International Journal of Molecular Sciences, 2013, 14(12):24169-24186. DOI PMID
[20]	张腾国, 赖晶, 李萍, 等. 不同处理下油菜RbohA、RbohD基因的表达特性分析[J]. 生态学杂志, 2019, 38(1):173-180.
	ZHANG Tengguo, LAI Jing, LI Ping, et al. Expression analysis of RbohA and RbohD genes in Brassica campestris under different treatments.[J]. Chinese Journal of Ecology, 2019, 38(1):173-180.
[21]	Chehab E W, Patharkar O R, Hegeman A D, et al. Autophosphorylation and Subcellular Localization Dynamics of a Salt- and Water Deficit-Induced Calcium-Dependent Protein Kinase from Ice Plant[J]. Plant Physiology, 2004, 135(3):1430-1446. DOI PMID
[22]	Xu J, Tian Y, Peng R, et al. AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis[J]. Planta, 2010, 231(6):1251-1260. DOI PMID
[23]	Giammaria V, Grandellis C, Bachmann S, et al. StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling[J]. Planta, 2011, 233(3):593-609. DOI PMID
[24]	Lanteri M L, Pagnussat G C, Lamattina L. Calcium and calcium-dependent protein kinases are involved in nitric oxide- and auxin-induced adventitious root formation in cucumber[J]. Journal of Experimental Botany, 2006, 57(6):1341-1351. PMID
[25]	足木热木·吐尔逊, 陈勋基, 陈果, 等. 玉米ZmCPK9基因在非生物胁迫下的表达分析[J]. 新疆农业科学, 2017, 54(9):1606-1612. DOI
	Zumuremu Turxun, CHEN Xunji, CHEN Guo, et al. Expression Analysis of ZmCDPK9 Gene in Maize under Abiotic Stress[J]. Xinjiang Agricultural Sciences, 2017, 54(9):1606-1612. DOI
[26]	李建平, 足木热木·吐尔逊, 常晓春, 等. 玉米钙依赖蛋白激酶38(ZmCDPK38)的生物信息学及表达特性分析[J]. 新疆农业科学, 2021, 58(1):49-55. DOI
	LI Jianping, Zhumuremu Turxon, CHANG Xiaochun, et al. Expression Characteristics and Bioinformatics Analysis of ZmCDPK38 Gene in Maize[J]. Xinjiang Agricultural Sciences, 2021, 58(1):49-55. DOI
[27]	Yan S, Weng B, Jing L, et al. Effects of drought stress on water content and biomass distribution in summer maize(Zea mays L.)[J]. Frontiers in Plant Science, 2023, 14.
[28]	冯晓钰, 周广胜. 夏玉米叶片水分变化与光合作用和土壤水分的关系[J]. 生态学报, 2018, 38(1):177-185.
	FENG Xiaoyu, ZHOU Guangsheng. Relationship of leaf water content with photosynthesis and soil water content in summer maise[J]. Acta Ecologica Sinica, 2018, 38(1):177-185.

编辑推荐 0

Metrics

阅读次数

全文

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	7	0	0	64

来源	本网站	其他网站

次数	44	27
比例	62%	38%

摘要

183

最新录用	在线预览	正式出版

0	0	183

来源	本网站	其他网站

次数	40	143
比例	22%	78%

元件 Element	数量 Number	功能 Function	元件 Element	数量 Number	功能 Function
TATA-box	30	核心启动元件	GT1-motif	1	光响应元件
CAAT-box	26	核心启动元件	G-box	1	光响应元件
STRE	6	unknown	DRE core	1	unknown
MYC	5	unknown	AT-rich element	1	富含AT的DNA蛋白结合位点
as-1	3	unknown	A-box	1	顺式作用调控元件
CGTCA-motif	3	茉莉酸甲酯响应元件	W box	1	unknown
TGACG-motif	3	茉莉酸甲酯响应元件	CCGTCC motif	1	unknown
ARE	3	厌氧诱导元件	TCT-motif	1	光响应元件
ABRE	2	脱落酸响应元件	Box 4	1	参与光反应
ACE	2	光响应元件	G-Box	1	光响应元件
AAGAA-motif	2	unknown	WUN-motif	1	unknown
Sp1	2	光响应元件	H-box	1	unknown
AT~TATA-box	2	unknown	ERE	1	unknown
LTR	1	低温响应元件	CAT-box	1	分生组织表达元件
CCGTCC-box	1	unknown	WRE3	1	unknown
Myc	1	unknown

元件 Element	数量 Number	功能 Function	元件 Element	数量 Number	功能 Function
TATA-box	30	核心启动元件	GT1-motif	1	光响应元件
CAAT-box	26	核心启动元件	G-box	1	光响应元件
STRE	6	unknown	DRE core	1	unknown
MYC	5	unknown	AT-rich element	1	富含AT的DNA蛋白结合位点
as-1	3	unknown	A-box	1	顺式作用调控元件
CGTCA-motif	3	茉莉酸甲酯响应元件	W box	1	unknown
TGACG-motif	3	茉莉酸甲酯响应元件	CCGTCC motif	1	unknown
ARE	3	厌氧诱导元件	TCT-motif	1	光响应元件
ABRE	2	脱落酸响应元件	Box 4	1	参与光反应
ACE	2	光响应元件	G-Box	1	光响应元件
AAGAA-motif	2	unknown	WUN-motif	1	unknown
Sp1	2	光响应元件	H-box	1	unknown
AT~TATA-box	2	unknown	ERE	1	unknown
LTR	1	低温响应元件	CAT-box	1	分生组织表达元件
CCGTCC-box	1	unknown	WRE3	1	unknown
Myc	1	unknown