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Study on Microbiota Diversity of Maternal and Fetus at the Late Pregnancy of Kazakh Ewe
LI Xiaobin,MA Chen, LI Fengming, HUANG Xinxin,Yimamu Mireguli, ZANG Changjiang, LI Jiahao,LI Chao, MA Lixin, CAO Rui
2021, 58(5):
965-972.
DOI: 10.6048/j.issn.1001-4330.2021.05.021
【Objective】 The purpose of this experiment is to study the microbiota diversity of the flora of Kazakh ewe and fetal tissues, intestinal contents and related organs before delivery. 【Methods】 Five 4-year-old Kazakh ewe with average body weight of (55.64±4.37) kg and the same parity were selected. After euthanasia, tissue organ samples were isolated and collected and analyzed for bacterial diversity. 【Results】 (1) There were higher diversity of the saliva, smaller intestine contents and amniotic fluid in the ewes; the chao1 index were 1,042.02, 1,058.31, 1,011.22 and the ACE index were 1,069.18, 1,067.05, 1,014.17, respectively. (2)At the species classification level, the abundance of firmicutes was higher in the small intestine, cecal contents, umbilical cord and the ewe and lamb meconium, accounting for 79.08%, 65.94%, 49.66% and 35.69%, respectively. The bacteroides of saliva and lamb rumen was 26.98 and 51.80%, respectively. The proteobacteria of saliva and lamb rumen was 64.92% and 41.92%, respectively. (3)The results of aggregation showed that the similarity of the species in the rumen fluid, meconium and umbilical cord of the lamb was higher, the similarity of the species in the saliva of the ewes and the rumen fluid was higher, and the similarity of the contents of the small intestine of the ewes and the cecal contents of the ewe were higher. 【Conclusion】 Therefore, the saliva, small intestine contents and amniotic fluid of the ewes have higher diversity index in the late pregnancy, and the thick-walled bacteria, the proteobacteria and the bacteroides are the main species of the tissues and organs of the ewes and fetuses at this stage.
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