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Response of Winter Wheat Canopy Structure and Microclimate to Planting Density in Walnut－Wheat Intercropping Pattern ZHANG Yongqiang, ZHANG Heng, FANG Hui, CHEN Chuanxin, Sailihan Sai, XUE Lihua, CHEN Xingwu, LEI Junjie 2020, 57(7): 1177-1186.  DOI: 10.6048/j.issn.1001-4330.2020.07.001 Abstract ( )   PDF (1564KB) ( )   【Objective】 To explore the effects of planting density on canopy structure and microclimate of winter wheat in walnut-wheat intercropping pattern.【Method】 In 2016-2017, planting densities of 4.5 million plants /hm2 (M1), 5.25 million plants /hm2 (M2), 6 million plants /hm2 (M3), 6.75 million plants /hm2 (M4) and 7.5 million plants /hm2 (M5) were set in walnut-wheat intercropping fields and canopy structure, canopy air temperature, humidity and canopy light transmittance of winter wheat were observed. 【Result】The results showed that the single leaf area, plant height and stem diameter of winter wheat in the distal crown region were higher than those in the lower crown region. With the increase of planting density, the leaf area, internode length and internode roughness of winter wheat in the subcrown and distal crown showed a trend of "first increasing and then decreasing", and the plant height varied from 76.49-81.66 cm (subcrown) to 78.34-86.27 cm (distal crown). The air temperature in the lower canopy area and the far canopy area showed a curve of "first rising and then falling". The M1 treatment in the lower canopy area was the highest, while the M4 and M5 treatment in the far canopy area were relatively high. The humidity in the far canopy area decreased slowly in the morning and rose slowly in the afternoon, and the humidity trough lasted for a short period. The variation amplitude of the canopy air humidity in the lower canopy area (44.73%-100%) was higher than that in the far canopy area (36.62%-100%).The incidence photosynthetic effective radiation (PAR) at the top of wheat canopy was significantly lower than that in the far canopy. The photosynthetic effective radiation interception (IPAR) of the canopy in the lower and far canopy areas showed a trend of "first rise and then fall" with the increase of density, and reached the maximum value in M2 treatment.【Conclusion】 It was concluded that the canopy structure and microclimate of winter wheat in walnut-wheat intercropping were more suitable when the planting density was 5.25 million plants /hm2.

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Expression of Grain Development Related Genes in Wheat ZY96-3 during Grain Filling Stage YANG Shuai, REN Mingjian, LI Zhenhua, LI Luhua, XU Ruhong 2020, 57(7): 1187-1194.  DOI: 10.6048/j.issn.1001-4330.2020.07.002 Abstract ( )   PDF (875KB) ( )   【Objective】 　To explore the expression pattern of ZY96-3 genes related to grain development during grain filling stage. in order to provide reference for further understanding of the molecular mechanism of gene regulation in grain filling. 【Method】 The real-time fluorescence quantitative PCR (qRT-PCR) technique was used to analyze the expression dynamics of 6 genes related to grain development during grain filling stage of ZY96-3. 【Result】The results showed that the expression patterns of six target genes of ZY96-3 increased first and then decreased, and all of them began to express 7 days after anthesis; TaTGW6 genes were involved in grain weight on the 7 d after flowering to express the highest amount, and the grain size TaCYP78A5 related genes, protein synthesis genes TaPBF - D and starch synthase gene GBSS Ⅰ, SBE1, AGPase1 all around 15 d after flowers express maximum amount; However, the expression level of each gene decreased significantly at 19 d after anthesis.【Conclusion】 This study showed that the grain-weight related gene TaTGW6 mainly played a key role in the early stage of wheat ZY96-3 grain filling, while the other 5 genes mainly played a role in the middle and late stage of wheat grain filling.

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Effects of Planting Density on Seed Quality of SafflowerHarvesting Sites in Xinjiang ZHOU Yuanhang, ZENG Cuiyun, WANG Huizheng, GUO Jianfu, MA Xiaolong, DU Tao, CHEN Yong 2020, 57(7): 1195-1200.  DOI: 10.6048/j.issn.1001-4330.2020.07.003 Abstract ( )   PDF (717KB) ( )   【Objective】 The effects of different planting densities in the surrounding areas of Manas County on different varieties of safflower harvest sites were discussed in order to provide some theoretical support for breeding methods such as safflower germplasm resource selection and breeding.【Method】 Xinjiang safflower variety Xinhonghua No. 4 was selected as the test material, and 5 seeding densities (1.5×104, 1.6×104, 1.8×104, 2.0×104, 2.2×104 plants / 667m2 ) with 3 repetitions and 15 cell processing were set. Under the same growth conditions of water and fertilizer and under different planting densities, the safflower seed yield and branch number, flower ball number, seed length, seed width, seed thickness, thousand seed weight, seed oil content and fatty acid content were determined.【Result】There are differences in the seed characteristics of different harvesting parts. The thousand seed weight, oil content and fatty acid content of the safflower harvested in the lower part were larger than those in the middle and top, and the top harvest index was slightly higher than that of the middle harvest. Under different planting densities, there was a negative correlation between the quality of the seeds and the number of branches in different parts of the safflower. Different planting densities affected the quality and yield of safflower harvesting parts. When the planting density reached 2.0×104 plants / 667m2, the top, middle and lower safflower seeds had the highest grain weight and yield, and the overall quality advantage was significant.【Conclusion】 The best planting density of Xinhonghua No. 4 in Manas County was 2.0×104 plants / 667m2. In the process of safflower breeding and production practice, scientific and reasonable planting density should be selected as far as possible, and the lower safflower seeds were used as propagation materials in order to achieve high quality, high yield and high efficiency.

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Analysis of Crude Fat Accumulation Mode and Fatty Acid Composition of Sunflower LEI Zhonghua, HUANG Qixiu, ZHANG Li, WU Wei, YANG Hui, SUN Xun, XIANG Lijun 2020, 57(7): 1201-1210.  DOI: 10.6048/j.issn.1001-4330.2020.07.004 Abstract ( )   PDF (1290KB) ( )   【Objective】 The content of fatty acids and the proportion of each component in oil crops are important standards to measure their oil use value, and it is of great significance to study the formation of fatty acids in oil crops. 【Methods】 Five different types of sunflower materials planted in different ecological areas were selected as the research objects. Through the determination of crude fat and fatty acid components, correlation and principal component analysis, the effects of different genotypes and ecological conditions on the fatty acid accumulation of sunflower were revealed.【Result】 The results showed that the synthesis rate of total fatty acids in the early stage of embryo formation was higher, and the accumulation amount in the early stage could reach more than 20% of the total amount, while the accumulation amount in the later stage was generally within 15%, and the synthesis rate was significantly lower than that in the early stage. The synthesis rate of oleic acid has the same trend, except for individual varieties, the accumulation amount in the early stage can account for more than 30% of the total fatty acid, which is significantly higher than that in the later stage. The decrease of temperature in the later stage of seed maturity resulted in the decrease of the accumulation rate of oleic acid in seed. 【Conclusion】 The total amount of final fatty acids in sunflower was closely related to the duration of the rapid growth of fatty acids during the development of seed embryo; The high oleic acid varieties kept high oleic acid synthesis rate in the later stage.

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Effects of Continuous Cropping on Agronomic Shape and Yield of Sweet Sorghum No.3 Zaituniguli Kuerban , Tuerxun Tuerhong, ZHU Min, TU Zhendong, Aikebaier Yilahong 2020, 57(7): 1211-1222.  DOI: 10.6048/j.issn.1001-4330.2020.07.005 Abstract ( )   PDF (1900KB) ( )   【Objective】 To study the effects of continuous cropping on the main agronomic characters, yield and quality of sweet sorghum were studied, and select the best way of continuous cropping fertilization in the hope of providing scientific basis for rational fertilization and nutrient management of continuous cropping sweet sorghum in arid area of Xinjiang.【Method】 Based on the long-term positioning experiment of sweet sorghum, the effects of two-year continuous cropping of sweet sorghum on the growth period, plant height, stem diameter, number of internodes, individual plant weight, culm weight and its sugar brix, straw yield, grain yield, thousand grain weight and biological yield of sweet sorghum variety New Sorghum No.3 were measured.【Result】Continuous cropping inhibited the growth of New Sorghum 3, reduced the yield and delayed the growth period. In the second year of continuous cropping, plant height, stem diameter, individual plant weigh and culm weight of all fertilization treatments were significantly lower than those in the first year. In the second year of continuous cropping, the crops by all fertilization treatments ripened 15 days later than the first year. In the second year of continuous cropping, the average straw yield, grain yield and biological yield decreased by 1.51%, 17.81% and 2.31% respectively. In addition, different fertilization treatments also have effects on the agronomic characters, sugar brix and yield of sweet sorghum. The application of different types of fertilizer could significantly increase the brix of sweet sorghum stalk. No application of any fertilizer (CK) significantly inhibited the growth and quality of New Sorghum 3. The plant height, number of internodes and stem diameter of NPK treatment were higher than those by other treatments during continuous cropping, with an increase rate of 1.80% - 8.90%. The yield and thousand grain weight of NPK treatment were as high as 4.46 t/hm2 and 21.54 g, while the brix and biomass of PK treatment were as high as 20.60% and 89.73 t/hm2.【Conclusion】 The results of comprehensive analysis showed that although continuous cropping inhibited the growth of sweet sorghum and reduced the main agronomic characters and yield, fertilization could reduce the growth barriers of continuous cropping to sweet sorghum to a certain extent. The main growth parameters of NPK treatment were the highest.

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Application Progress of CRISPR / Cas9 Technology in Horticultural Crops HUANG Shaoyong , WANG Juan, WANG Baike , LI Ning , TANG Yaping , YANG Shengbao, YANG Tao, ZHANG Guoru, Patiguli Aisimutuola, GAO Jie, YU Qinghui 2020, 57(7): 1223-1232.  DOI: 10.6048/j.issn.1001-4330.2020.07.006 Abstract ( )   PDF (909KB) ( )   【Objective】 To review the application of gene editing technology in horticultural crops in recent years in the hope of providing a reference for the basic research and variety cultivation of horticultural crops. 【Methods】 The progress report of CRISPR/Cas9 technology in horticultural crops research was collected from domestic and foreign literature. After that the relevant analyses were summarized. 【Results】 Traditional breeding methods were difficult to meet the growing demand for yield and quality of horticultural crops, and CRISPR/Cas9 technology opened up a new way for its variety improvement, which would completely change the breeding pattern of horticultural crops, and make a significant breakthrough in resisting biological stress, responding to abiotic stress, improving fruit quality and crop domestication. 【Conclusion】 Great progress has been made in CRISPR/Cas9 technology in horticultural crop research and is an indispensable key technology for future breeding work.

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Cloning, Expression Analysis and Prokaryotic Expression of Lavender DXS Gene GONG Lintao, SU Xiujuan, YIN Songsong, SUN Minghui, YAN Bowen, Adilai Abdur aimu 2020, 57(7): 1233-1242.  DOI: 10.6048/j.issn.1001-4330.2020.07.007 Abstract ( )   PDF (3471KB) ( )   【Objective】 This study intends to clone the lavender DXS gene and analyze its expression preliminarily in the hope of providing a research basis for revealing the molecular mechanism of this gene in regulating lavender terpenoid synthesis. 【Methods】 Lavender "Zahua" was taken as the testing material to clone lavender DXS gene,and then gene sequence analysis was conducted, expression comparison performed and prokaryotic expression finished. 【Results】 (1)The open reading frame length of lavender DXS gene was 2,181 bp, encoding a protein sequence consisting of 726 amino acids, with an isoelectric point of 6.57 and a molecular weight of about 78.39 KDa. Lavender DXS protein was highly conserved and closely related to the DXS protein of Lavandula angustifolia, Isodon rubescens, and Plectranthus barbatus. (2) The expression level of DXS gene was the highest in the decaying period of Zahua organs and the highest in the blooming period of French blue flower organs. The expression level of DXS gene in the five different developmental stages of Zahua organs was higher than that of French blue. The expression level of DXS gene was the highest in the calyx of Zahua and the highest in the stamens of French blue. The expression level of DXS gene in five different tissues of hybrid flowers was higher than that in French blue (except pistil and stamen). (3) The results of prokaryotic expression analysis showed that the expression of DXS protein was the largest after 4 h induction at 37℃ and IPTG 0.8 mM. 【Conclusion】 There is a positive correlation between DXS gene expression and lavender essential oil production. The results of this study provide material basis and experimental conditions for further confirming the function of DXS gene in the metabolism of lavender terpenoids.

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Comparison of Fruit Quality, Yield and Photosynthetic Characteristics of Walnut Varieties Xinxin 2 and Wen 185 Abduxukur Yakup, Muhtar Zari, MA Kai, ZHANG Qiang, HUANG Minmin, NING Wanjun 2020, 57(7): 1243-1250.  DOI: 10.6048/j.issn.1001-4330.2020.07.008 Abstract ( )   PDF (907KB) ( )   【Objective】 To reveal the relationship between photosynthetic characteristics with quality and yield of walnuts.【Method】 Xinxin 2 and Wen 185 were taken as experimental materials to analyze the relationship between photosynthetic and chlorophyll fluorescence characteristics of the leaves with yield and fruit quality of the two walnut varieties.【Result】There was no significant difference in photosynthetic rate and quantum efficiency between Wen 185 and Xinxin 2, which was consistent with the yield and yield per plant of the two varieties. There was a significant difference in the rate of kernel yield between the two varieties. The rate of Wen 185 kernel was significantly higher than that of Xinxin 2, and the respiratory rate was also significantly higher than that of Xinxin 2. The rate of kernel yield may be related to its metabolic characteristics. There was no significant difference in total chlorophyll content between the two varieties, but the chlorophyll a/b and carotenoid content of Wen 185 were significantly higher than those of Xinxin 2.【Conclusion】 The yields of Wen 185 and Xinxin 2 were related to their photosynthetic characteristics, and their qualities were related to other metabolism. Wen185 may have stronger light protection capability than Xinxin 2.

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Effects of Chemical Blossom Thinners and Cost on Xinhong 1 Fuji Apple WANG Anli, LI Wensheng, ZHOU Wenjing, WU Zezhen, ZHANG Zhenjun, HU Anhong 2020, 57(7): 1251-1258.  DOI: 10.6048/j.issn.1001-4330.2020.07.009 Abstract ( )   PDF (715KB) ( )   【Objective】 Comparison To compare the effect and cost of different chemical blossom thinners on Red Fuji apples, and find suitable chemicals and itsand concentration which can well regulate the load and improve quality and reduce costs in Aksu. 【Methods】 Taking the initial fruit stage and high yield stage Xinhong No. 1 as the test material, several blossom thinning treatments were evaluated, including hand-thinning (control), stone sulphur, ethephon, calcium formate, and thinner. Chemical treatments were applied when 75%-85% of center flower and 70%-85% of whole tree flower was blooming. Compared with hand thinnedthinning, the effects of thinning and cost were analyzed. 【Results】 Different concentrations of stone sulphur mixture, ethephon, calcium formate and thinner had different thinning effects. Compared with the control, there are differences in cost., The cost varies greatly. 【Conclusion】 Through the comprehensive comparison of fruit setting rate of inflorescence, fruit setting rate of flower, percentage of single fruit, empty platform rate and removal rate, the effect of the stone sulphur mixture 200 times solution in the initial fruit stage was the closest to artificial fruit thinning, and the cost was 17.08% of human labor. The effect of the stone sulphur mixture 150 times solution in the full bearing period was the closest to artificial fruit thinning, and the cost was 24..79% of human labor. The cost of chemical thinning was positively correlated with the price of thinning and tree age.

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Effects of Different Remaining Fruit Spikes on Leaf Photosynthetic Characters, Yield, and Quality of Tomato produced in Gobi Greenhouses SONG Yu, JIANG Chengyao, LI Yushan 2020, 57(7): 1259-1266.  DOI: 10.6048/j.issn.1001-4330.2020.07.010 Abstract ( )   PDF (3607KB) ( )   【Objective】 To investigate the effects of different remaining fruit spikes on leaf photosynthetic characteristics and fruit quality of tomatoes growing in Gobi solar greenhouses in southern Xinjiang in the hope of obtaining the economic optimal tomato fruit number left for Gobi greenhouse tomato production. 【Method】 NS3389 tomato was used as the test material, and four kinds of remaining spikes were set (T3: three spikes; T4: four spikes; T5: five spikes; T6: six spikes). Leaf photosynthetic characteristics, plant yield, and fruit quality of tomatoes were monitored to figure out better beneficial fruit remaining spikes. 【Result】 Except for T3 and T6 treatments, the photosynthetic pigment content in functional leaves of tomato with different numbers of remaining fruit spikes generally increased with the increase of the number of retained fruit. The net photosynthetic rates (PN) were significantly higher under those of T4 and T5 treatments, while the stomatal conductance (GS), intercellular CO2 concentration (Ci), and transpiration rate (Tr) of tomato leaves under T5 and T6 treatments were higher than those of other treatments. The data of fruit yield per plant in T6 treatment was significantly higher than those in other treatments, and the annual yield of greenhouse at T4 and T5 treatments were the highest. There was no significant difference in the fruit shape index among the treatments, while the contents of soluble solids, ascorbic acid (VC), and lycopene of fruits under T4 and T5 treatments were significantly higher than those under other treatments. 【Conclusion】 According to comprehensive yield and quality analysis, tomato production in Gobi greenhouses in southern Xinjiang is more suitable to adopt 4 and 5 spike fruit retention modes.

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Selection of Main-Planted Tomato Varieties in Xinjiang SONG Fangyuan, ZHAO Zhiyong, LI Jixin 2020, 57(7): 1267-1275.  DOI: 10.6048/j.issn.1001-4330.2020.07.011 Abstract ( )   PDF (1603KB) ( )   【Objective】 To screen out the processed tomato varieties with excellent quality in Xinjiang. 【Methods】 In this study, 17 processed tomato varieties were taken the experimental materials and their changes of growth period, soluble solids, fruit hardness, fruit hanging time, decay rate and pressure tolerance of 17 early, middle and late ripening varieties were studied. 【Results】 The results showed that the varieties with different maturation stages were more concentrated, with a difference of 7-15 days. The time of hanging branches was inversely proportional to the rate of decay. The lower the rate of decay, the longer the time of hanging branches. The order of the length of hanging branches was: precocious variety > medium-maturing variety > late-maturing variety; The order of soluble solids content of fruit during the whole period of red ripening was: late-maturing variety > medium-maturing variety > precocious variety;The fruit hardness of all varieties decreased gradually. The hardness of Shifan 28 of precocious varieties was the best, the best of medium-maturing varieties was Henshi 3402 andYaxin, and the best of late-maturing varieties were Henshi 9780 and Kenfan 2. 【Conclusion】 Three high-quality precocious varieties, Heinz 2206, Shifan 15 and Shifan 28 were selected by comprehensive comparison;Two medium-maturing varieties that were selected were: Yaxin 8 and SF227;Three late-maturing varieties that were selected were: Henshi 9780, Kenfan 2 and Kenfan 5.

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Synergistic Effect of Pretreatment and Cultural Method on Lilium ×fomolongi Germination WU Xiaomei, YUAN Yanping, WEI Xiaoru, CAO Fuxiang, CHEN Jiren, LÜ Changping, LI Yufan 2020, 57(7): 1276-1286.  DOI: 10.6048/j.issn.1001-4330.2020.07.012 Abstract ( )   PDF (1975KB) ( )   【Objective】 Lilium formolongi Raizan No.1 was used as experimental material, the orthogonal experiment design of three factors and four levels was used to research the effects of different seed pretreatments and cultural temperatures, and cultural method on Lilium formolongi Raizan No.1 seed germination. This experiment also researched the dynamic change of soluble sugars and superoxide dismutase (SOD) in different temperatures. 【Results】 The results showed that the key factor was cultural temperature, followed by pretreatment and cultural method. And as for Raizan No.1, the best way of pretreatment was soaking seed in 4℃ for 7 days, the optimum temperature was 21℃,the optimum culture mode was sowing in soil, its germination was 99.18% and the germination index was 4.65. At different temperatures, the activity of superoxide dismutase (sod) increased first and then decreased at germination stage, but the soluble sugar content showed a tendency of going down first and then up. Among them, the resistance of the seeds under 21℃ treatment and the conversion utilization of the stored substances were higher than those under 15℃,18℃ and 25℃ treatment, which confirmed that 21℃ was the appropriate temperature. 【Conclusion】 The optimum treatment of the Rayshan 1seeds was to sow at 21℃ for 4℃ for 7 days .

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Effects Fertilizer Application Amount in Substrate on Growth of Pepper Plug Seedlings XIE Yanru, TANG Dan, ZHANG Pu, ZHAO Zhixin, DONG Ruifang, CUI Yongmin, XU Youzhang, GUI Ruiqi, YE Lihong, LI Meichen, QIN Yong 2020, 57(7): 1287-1294.  DOI: 10.6048/j.issn.1001-4330.2020.07.013 Abstract ( )   PDF (803KB) ( )   【Objective】 To select the suitable amount of chemical fertilizer in the substrate for pepper(Capsicum frutescence L.)plug seedling cultivation in Hotan region of Xinjiang.【Method】 In this experiment, hot pepper variety Hangjiao S605 was selected as the testing material, coconut bran∶ fine sand∶ organic fertilizer=20∶1∶1 was used as seedling substrate formula to carry out pepper plug seedling experiments, and during seedling raising, urea, potassium hydrogen phosphate and potassium sulfate were fertilized according to different proportions; By observing and comparing the growth status and physiological indexes of pepper seedlings with different fertilizer application rates in the substrate, the effects of different fertilizer application rates on the growth of pepper plug seedlings were studied so as to screen out the fertilizer application amount suitable for the growth of pepper plug seedlings in Hotan region of Xinjiang.【Results】The result showed that when pepper seedlings were raised with the matrix formula of coconut bran∶ commercial organic fertilizer∶ sand = 20∶1∶1(volume ratio), treatment 2 (the application amounts of N,P2O5 and K2O are 200 ,200 , 200 g/m3, respectively) was superior to other treatments in stem diameter, dry weight and dry-fresh ratio. The strong seedling index of treatment 2 was the largest, 0.099,6; The strong seedling index of CK was 0.068,8. Many indexes of treatment 4 (the application amounts of N,P2O5 and K2O are 400, 400,400 g/m3, respectively) were the minimum, and strong seedling index was also the minimum, only 0.0556.【Conclusion】 Therefore, treatment 2 (the application amounts of N,P2O5 and K2O are 200, 200,200 g/m3, respectively) can be used as the amount of chemical fertilizer in the substrate for pepper plug seedling raising, which is beneficial to the growth of pepper seedlings.

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Study on the Change of Dry Matter Accumulation and the Contents of Crude Starch and Crude Protein in Lanzhou Edible Lily in Different Planting Areas LIN Yuhong, PEI Huaidi, LI Shujie, SHI Youtai 2020, 57(7): 1295-1304.  DOI: 10.6048/j.issn.1001-4330.2020.07.014 Abstract ( )   PDF (1878KB) ( )   【Objective】 To study the changes of dry matter accumulation and crude starch and crude protein content in edible lily of Lanzhou in different ecological regions and to explore the regional differences. 【Method】 The growth analysis method was used to detect the accumulation of dry matter and the changes of crude starch and crude protein content in lily bulbs.【Results】 The cumulative growth of dry matter of lily bulbs in different growth years of Yongjing County (YJ), Weiyuan County (WY) and Lintao County ( LT) planting areas was different. Compared with the three planting areas, the differences in dry matter accumulation of lily bulbs reached extremely significant levels (P<0.01). The cumulative amount of dry matter of Lanzhou edible lily bulbs in triennial was: LT>WY>YJ. The crude starch content LT of lily bulb was higher than those of YJ (24.74%) and WY (68.02%). The order of crude starch content was: LT>YJ>WY; the crude protein content LT of lily bulb was higher than those of YJ (8.55%) and WY (11.09%), the order of crude protein content was: LT>YJ>WY. The triennial Lanzhou edible lily, the dry matter accumulation of the WY and LT eco-regions was significantly positively correlated with the crude protein content (0.99**, 1.00**, P<0.01).【Conclusion】 The nutrient content of edible lily in different planting areas was different, and the difference between areas was significant.

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Screening and Optimization of Clarifiers for Thompson Seedless Wine CHEN Ya, LEI Jing, LIAN Weijia, HAN Chen, WU Bin, REN Hongsong 2020, 57(7): 1305-1313.  DOI: 10.6048/j.issn.1001-4330.2020.07.015 Abstract ( )   PDF (1692KB) ( )   【Objective】 To study the effect of clarifier on Thompson seedless wine and lay the foundation for producing high quality Thompson seedless wine. 【Method】 The effects of three factors including chitosan addition amount, bentonite addition amount, gelatin addition amount and their interaction on transmittance were studied by single factor test and response surface method. Regression equations were established and analyzed by significance and variance. The optimum clarification agent of Thompson seedless wine were then obtained and verified by experiment. 【Result】Among the five single clarifiers (chitosan, bentonite, gelatin, pectinase and phytic acid), chitosan, gelatin and bentonite had a better clarification effect on Thompson seedless wine. The addition amount of chitosan, bentonite and gelatin was used as single factor test. The Optimum clarifying agent of thompson seedless wine by single factor experiment and response surface method. The transmittance was 98.45%, which was basically consistent with the theoretical value (98.473,1%). 【Conclusion】 The best compound clarifiers for Thompson seedless wine were chitosan addition amount 0.09 g/L, bentonite addition amount 0.16 g/L, gelatin addition amount 0.11 g/L. Under these conditions, Thompson seedless wine has the best clarification effect.

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Effects of Nitrogen on the Activities of Key Enzymes for Nitrogen Assimilation of Salicornia europaea L. during Seedling Stage HUANG Jian, DUAN Jingjing, MA Xiaodong, QI Tong, FU Yanbo 2020, 57(7): 1314-1320.  DOI: 10.6048/j.issn.1001-4330.2020.07.016 Abstract ( )   PDF (1165KB) ( )   【Objective】 In order to reveal the nitrogen catalysis and assimilation capacity of Salicornia europaea L. seedlings under nitrogen application in salt environment, and to deeply understand the mechanism of the grass to nitrogen uptake and transformation. 【Method】 The pot experiment method was used to study the effects of different nitrogen application levels on the key enzyme activities of nitrogen assimilation of Salicornia europaea L. seedlings growing under salt environment. 【Results】 The results showed that, compared with the treatment without nitrogen application, under severe salt environment conditions, nitrogen application increased the activities of roots, stems, assimilated shoots NR, GS, GOGAT, GLDH of the plant. Under N 1.2 g/kg, the NR activity, GS, GOGAT activity of the root, stem, and assimilated branches of Salicornia europaea L. seedlings reached their maximum values; When N (2.4 g / kg) was applied, the roots and assimilated branches of GS, GOGAT activity and GLDH activity of root, stem and assimilated branches reached the maximum. 【Conclusion】 Nitrogen application in salt environment can significantly enhance the key enzyme activities of nitrogen assimilation of Salicornia europaea L. seedlings, increase its ability to nitrogen stress, thus enhancing its adaptability to high nitrogen environment.

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Study on the Classification of Qiaxi National Forest Park Based on the GF-2 Image SHI Yuxia, WANG Zhenxi, YANG Yongqiang, LIAN Ling, GAO Yali 2020, 57(7): 1321-1329.  DOI: 10.6048/j.issn.1001-4330.2020.07.017 Abstract ( )   PDF (1440KB) ( )   【Objective】 To select the object-oriented supervised classification method for remote sensing classification of spruce forest in Tianshan Mountain, and to select a method with the best classification effect in the hope of providing the basis for the investigation of forest land resources and dynamic monitoring and evaluation in this area. 【Methods】 Based on GF-2 remote sensing image data, the optimal segmentation scale of objects in the study area was screened by the combination of ESP scale evaluation tool and visual interpretation. On the basis of three different classification methods, remote sensing classification was conducted. 【Results】 The optimal segmentation scales of water body, road, other land, forest land and grassland in the study area were 390, 372, 316, 296 and 246, respectively. Secondly, under the optimal segmentation scale of local objects, compared with the nearest neighbor classification, combined with vector data classification and threshold classification, it was found that the Kappa coefficient and overall precision of the three classification methods were 0.760,7, 0.782,0, 0.840,6 and 0.814,8, 0.830,5 and 0.876,5, respectively. 【Conclusion】 The threshold classification method is superior to the other two classification methods, which indicates that it is feasible to select a better threshold classification method to solve the problem of forest land resources investigation in this area.

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Effects of Crop Distribution on Occurrence and Damage of Ostrinia furnacalis Population WANG Xiaowu, DING Xinhua, FU Kaiyun, LIU Fanghui, ZHANG Huiju, Tursun, GUO Wenchao 2020, 57(7): 1330-1337.  DOI: 10.6048/j.issn.1001-4330.2020.07.018 Abstract ( )   PDF (1197KB) ( )   【Objective】 Ostrinia furnacalis is the main pest on corn. The relationship between Ostrinia furnacalis occurrence and crop distribution was studied in the hope of providing a theoretical basis for ecological prevention and control of the pest.【Methods】 In the typical occurrence area of Ostrinia furnacalis in Shule County, the effect of crop distribution experiment was conducted in which the amount of moth, eggs dropping amount, larvae, alfalfa and the index of decay rate of stem, the number of holes on stalks and cluster and living insects were used to evaluate the population dynamics and damage of Ostrinia furnacalis under maize+cotton (Tr1), maize+melon (Tr2) and maize+wheat (Tr3) intercrop patterns.【Results】In terms of population dynamics, there were some differences in the amount of trapped moths, dropped eggs, larvae and alfalfa under different crop distribution (P < 0.005). The amount of moth (overwintering, 1st and 2nd generations), eggs, larvae and alfalfa of Tr1 adjacent field were significantly higher than those of Tr2 and Tr3 (P < 0.005); Moreover, the amount of dropped moths, dropped eggs, larvae and alfalfa in the Tr2 adjacent field were higher than those in the Tr3, while the difference was not significant (P > 0.005); In terms of hazard: There were some differences in the occurrence and damage of Ostrinia furnacalis (P < 0.005) under different crop distribution; In addition, the average rate of corn stalk rate was 18.03% and the difference was significant (P < 0.005), and the total number of holes and living insects from large to small was Tr1>Tr3>Tr2 and Tr1>Tr2>Tr3. 【Conclusion】 After emergence of overwinting adults of the Ostrinia furnacalis, they preferred to migrate to the Tr1 neighboring field and the 1st and 2nd generation adults preferred the field of corn planting around the cotton. Above all, occurrence and damage of the Ostrinia furnacalis could be regulated by crop distribution and rational chemical control.

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Effects of Forest Conditions and Stand Structure on the Quantity of Agrilus mali Mats in Tianshan Wild Apple Forest MA Zhilong, PENG Bin, Caddey Kader, ZHANG Yuanming, Adili Sattar 2020, 57(7): 1338-1348.  DOI: 10.6048/j.issn.1001-4330.2020.07.019 Abstract ( )   PDF (1665KB) ( )   【Objective】 To understand the occurrence of natural quantity of Agrilus mali Matsumura in Tianshan wild apple forest and its relationship with influencing factors.【Methods】 This study was based on 17 standard plots of wild apple trees in Tianshan Mountains, taking Agrilus mali Matsumura as the object of study to study the effects of site factors such as altitude, gradient, slope position and aspect, forest stand factors such as stand canopy, stand density, wild apples ratio and tree species richness on the quantity of Agrilus mali Matsumura, and to build the prediction mode.【Results】The results showed that: The average quantity of insect in stands with an altitude higher than 1,400 m was the lowest, only 0.49 /m sample branch, the average insect quantity was the highest in the range of 1,250-1,299 m, up to 1.26 /m sample branch. With the increase of the gradient of the sample land, the quantity of Agrilus mali Matsumura was decreasing, the average insect quantity in the gentle slope stands(6°≤gradient≤15°) was the highest, up to 1.24 /m branches, which was significantly higher than the 0.39 /m sample branches on the steep slope (26°≤ gradient ≤35°). The highest number of insect quantity was found in stands with canopy density of 0-0.29, up to 1.44 /m branches, it was significantly higher than stand density of 0.30-0.49 and 0.50-0.79 (P＜0.05). The average number of insect quantity in sparse stands with stand density lower than 0.11 was the highest, which was 1.5/m sample branches. Partial correlation analysis showed that the absolute value of partial correlation coefficient between elevation and slope was larger, was 0.598 and 0.542, it was the key influencing factor of the quantity of Agrilus mali Matsumura. T test results showed that the relationship between them and the average quantity reached a significant level(PX1=0.031;PX3=0.047;＜0.05). The multiple regression model: Y=5.541-0.003X1-0.033X4 was established based on altitude (X1), slope position (X4) and number of quantity (Y). F test shows that the linear regression prediction model reaches a significant level(F=12.021, df=2, 16, P=0.001). The predicted value was compared with the measured value and the average difference was 0.193. The measured value and the predicted value were tested by T, the results showed that there was no significant difference. 【Conclusion】 Altitude and gradient are the key factors that affect the amount of Agrilus mali Matsumura, slope position is the second, the effects of stand canopy, stand density, slope direction, wild apple ratio and tree species richness on the number of Agrilus mali Matsumura population are weak, and they are non-major factors.

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Morphological Observation of Hermetia illucens and Preliminary Screening of Oviposition-inducing Bacteria JING Haiquan, ZHONG Wen, ZHAN Faqiang, YANG Rong, BAO Huifang, HOU Min, LONG Xuanqi 2020, 57(7): 1349-1360.  DOI: 10.6048/j.issn.1001-4330.2020.07.020 Abstract ( )   PDF (2765KB) ( )   【Objective】 To ascertain the life history and the change rule of male-female ratio of adult Hermetia illucens population under indoor feeding conditions, screen and preliminary identify oviposition-inducing bacteria from the egg surface and larval intestinal tract in the hope providing theoretical basis for the large-scale breeding and comprehensive utilization of Hermertia illucens in local area.【Method】 Hermetia illucen introduced has been reared 4 generations indoors, its life span and life habits in different diets were recorded by direct observation methods, morphological character was observed with microscope, oviposition-inducing bacteria from the egg surface and larval intestinal tract were separated and purified by dilute coating flat plate and flat plate scribing method, and were screened and preliminary identified as the bacteria afterwards.【Result】Compared with interior area, the period from eggs to adults was slightly prolonged slightly in Xinjiang, but it was developed well and could be reared for generations in this region. Eggs, larvae, pupae, adults and their genital morphology were observed and recorded. Two strains of bacteria named e1 and Y3 with oviposition-inducin effect were screened. The fermentation broth treatment could increase oviposition rate by 6.26 times and 7.40 times respectively compared with the control. The two strains have been tested as Gram-positive bacteria by Gram staining, and preliminary identified as Bacillus siamensis and Athrobacter respectively with methods of 16S RNA sequencing. Y3 was regarded as a potential new bacteria.【Conclusion】 Annual circular aquaculture of Hermetia illucens in Xinjiang is feasible. Hermetia illucens also has the potential of large-scale production and comprehensive development and utilization.

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A Visual Analysis of Informatization Related Papers of Xinjiang Agricultural Sciences Based on Citespace Method MA Jihong, TIAN Changyan, LÜ Guanghui, GUO Wenchao 2020, 57(7): 1361-1370.  DOI: 10.6048/j.issn.1001-4330.2020.07.021 Abstract ( )   PDF (1911KB) ( )   【Objective】 To know the informatization construction, technological evolution and hot spots of agricultural science and technology journals in China in the hope of providing basis for the publication of agricultural sci-tech journals. 【Method】 Based on CNKI, using Citespace and bibliometrics, this paper made a visual analysis of the related papers of periodical informatization including agriculture, constructed the evolution map of the main nodes of the periodical informatization keywords co-occurrence area map and information technology network, and visualized its information research intuitively, application and development process. 【Result】In the CNKI database, there were 2,472 articles related to journal informatization from 2000 to 2019. The keyword network had 304 nodes and 1,718 network lines. The representative key nodes were networking, digitalization, new media, big data, Wechat public platform and media integration according to the time sequence.Among the key words co-occurrence clustering, main clusters were wechat, internet and other information technologies. The frequency distribution of key words also reflected that new media technology and its integration were the mainstream of the current journal informatization construction and publication. There was only “new media” node in the high frequency of related literature keywords in the network for informatization construction of science and technology periodicals. 【Conclusion】 Although agricultural sci-tech journals have been improved in informatization construction, they still lag behind the overall development of Chinese academic journals. At the same time of speeding up informatization and digitalization, Xinjiang Agricultural Sciences has improved its publishing and academic communication ability through new media operation, and has been at the forefront of the informatization construction of agricultural journals in China. For improving its academic communication ability and influence, media integration and intelligent publishing may be an important direction for the informatization construction and publication of this journal in the next stage in the future.