Study on Purification of Total Flavonods from Fruit of Pomegranate Cultivars of Xinjiang by D-101 Macroporous Adsorption Resin

doi:10.6048/j.issn.1001-4330.2019.06.012

Xinjiang Agricultural Sciences ›› 2019, Vol. 56 ›› Issue (6): 1095-1103.DOI: 10.6048/j.issn.1001-4330.2019.06.012

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Study on Purification of Total Flavonods from Fruit of Pomegranate Cultivars of Xinjiang by D-101 Macroporous Adsorption Resin

MENG Xin-tao^{1, 2}, WEI Jian³, ZHANG Ting^{1, 2}, LI De-hua⁴, TAN Gui⁴, ZHU Li-na⁴, SHAO Wen-zhi⁴, PAN Yan^{1, 2}

1. Research Institute of Agro-products Storage and Processing, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
2. Research center of Main Agricultural Products Processing Engineering in Xinjiang, Urumqi 830091,China;
3. College of Life and Geography Sciences, Kashi University, Kashi Xinjiang 844006, China;
4. College of Food Sciences and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China

Online:2019-06-20 Published:2019-08-08
Correspondence author: PAN Yan(1979-),male,doctoral candidate,research associate,engaged in farm product storage and processing, (E-mail)panyan1590@163.com
Supported by:
Supported by National Science and Technology Support Project (2011BAD27B01-01-02) and Outstanding Young Scientific Talent Foundation of Xinjaing Academy of Agricultural Sciences (xjnkq-2015038)

基于D-101大孔吸附树脂分离纯化新疆石榴皮中的总黄酮

孟新涛^{1, 2}, 魏健³, 张婷^{1, 2}, 李德华⁴, 谭归⁴, 朱丽娜⁴, 邵文志⁴, 潘俨^{1, 2}

1.新疆农业科学院农产品贮藏加工研究所, 乌鲁木齐 830091;
2.新疆主要农副产品精深加工工程技术研究中心, 乌鲁木齐 830091;
3.喀什大学生命与地理科学学院,新疆喀什 844006;
4.食品科学与药学学院,新疆农业大学,乌鲁木齐 830052

通讯作者: 潘俨(1979-),男,副研究员,博士研究生,研究方向为农产品贮藏加工与保鲜,(E-mail)panyan1590@163.com
作者简介:孟新涛(1985-),女,助理研究员,硕士研究生,研究方向为农产品贮藏加工与保鲜,(E-mail)mengxintao_929@126.com
基金资助:
国家科技支撑项目(2011BAD27B01-01-02);新疆农业科学院青年基金(xjnkq-2015038)

Abstract

Abstract: 【Objective】 In this study, D-101 macroporous adsorption resin was used to separate and purify total flavonods from fruits of pomegranate cultivars from Xinjiang.【Method】 Based on the single factor study, the conditions for the separation and purification of total flavonoids from pomegranate skin by DX101 macroporous adsorption resin were further studied by investigating the temperature, pH, adsorption penetration curve, eluent concentration and ratio of material to liquid, and the optimum technological conditions were found.【Result】The optimal purification conditions were as follows:pH 3-4,temperature of 30℃, material ratio of 1∶4(g/mL), the volume of ethanol in the eluent was 90%. Under this process condition, the purity of total flavonoids in pomegranate peel increased from 7.25% to 17.32%. 【Conclusion】Under this process condition, the non-target ingredients, such as pigment and chlorophyll can be eluted effectively, and the total flavonoids of pomegranate cultivars of Xinjiang can be separated and purified scientifically and reasonably and the operation is simple, safe and of low cost.

Key words: pomegranate; peel; flavonoid; D-101

摘要： 【目的】研究采用D-101大孔吸附树脂分离纯化新疆石榴皮中的总黄酮。【方法】以单因素为研究基础,分析温度、pH、吸附穿透曲线及洗脱液浓度、料液比等因素,研究D-101大孔吸附树脂分离纯化石榴皮总黄酮的条件,并找到最佳工艺条件。【结果】D101大孔吸附树脂对石榴皮中黄酮类物质纯化的最佳工艺为:提取液pH为4、温度30℃、料液比1∶4(g/mL)、洗脱液乙醇体积为90%。该工艺富集纯化石榴皮黄酮类物质效果较好,石皮总黄酮的纯度由7.25％提高至17.32％。【结论】能有效的洗脱色素、叶绿素等非目标成分,科学合理的分离纯化石榴皮中总黄酮,且操作简单、安全、成本低廉。

关键词: 石榴, 分离纯化, 黄酮, D-101

CLC Number:

MENG Xin-tao, WEI Jian, ZHANG Ting, LI De-hua, TAN Gui, ZHU Li-na, SHAO Wen-zhi, PAN Yan. Study on Purification of Total Flavonods from Fruit of Pomegranate Cultivars of Xinjiang by D-101 Macroporous Adsorption Resin[J]. Xinjiang Agricultural Sciences, 2019, 56(6): 1095-1103.

孟新涛, 魏健, 张婷, 李德华, 谭归, 朱丽娜, 邵文志, 潘俨. 基于D-101大孔吸附树脂分离纯化新疆石榴皮中的总黄酮[J]. 新疆农业科学, 2019, 56(6): 1095-1103.

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Study on Purification of Total Flavonods from Fruit of Pomegranate Cultivars of Xinjiang by D-101 Macroporous Adsorption Resin

基于D-101大孔吸附树脂分离纯化新疆石榴皮中的总黄酮

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