Study on Purification Process of Total Flavonoids from Armeniaca sibirica (L.) Lam by Macroporous Resin

doi:10.6048/j.issn.1001-4330.2022.04.014

Abstract

Abstract:

【Objective】 To optimize the optimal process parameters for separation and purification of total flavonoids from Armeniaca sibirica (L.) Lam with macroporous resin.【Methods】 Ultraviolet spectrophotometry was used to determine the total flavonoids content of Armeniaca sibirica (L.) Lam, and the static adsorption and desorption test was used to investigate the adsorption and desorption capacity of 4 different types of macroporous resins on the total flavonoids of Armeniaca sibirica (L.) Lam. The appropriate macroporous resin was selected and finally the best separation and purification conditions were determined.【Results】 D101 type macroporous resin had a better specific adsorption capacity and desorption capacity for total flavonoids from Armeniaca sibirica (L.) Lam. The best separation and purification process conditions were D-101 macroporous resin with a diameter-to-height ratio of 1∶6, and the loading volume of the crude extract of Armeniaca sibirica (L.) Lam was the largest: 6.3 BV, whose best loading concentration was 0.3 mg/mL, The loading rate was 1.0 mL/min, the standing time was 2 h, the washing volume was 5 BV, and the washing liquid elution flow rate was 1.5 mL/min. 80% ethanol was used as the elution solvent, whose amount was 2 BV, and the eluent flow rate 0.5 mL/min.【Conclusion】 D101 type macroporous resin can be used for the separation and purification of total flavonoids in Armeniaca sibirica (L.) Lam under the obtained technological conditions.

Key words: Armeniaca sibirica (L.) Lam; total flavonoids; D-101 macroporous resin; separation and purification; adsorption

摘要：

【目的】研究优选大孔树脂分离纯化野山杏总黄酮的最佳工艺参数。【方法】采用紫外分光光度法测定野山杏总黄酮含量,利用静态吸附解吸试验分析4种不同型号大孔树脂对野山杏总黄酮的吸附及解吸能力,选出适宜的大孔树脂及其最佳分离纯化条件。【结果】D101型大孔树脂对野山杏总黄酮有较好的比吸附量和解析附能力,最佳分离纯化工艺条件为D-101大孔树脂径高比1∶6,野山杏粗提液最大上样量为6.3 BV,最佳上样浓度为0.3 mg/mL,上样速率为1.0 mL/min,静置时间为2 h,水洗体积为5 BV,水洗液洗脱流速1.5 mL/min,洗脱溶剂用80%乙醇,洗脱溶剂用量2 BV,洗脱剂流速为0.5 mL/min。【结论】D101型大孔树脂在所得工艺条件下可以对野山杏中总黄酮进行较好的分离纯化,可用于野山杏总黄酮的分离纯化。

关键词: 野山杏, 总黄酮, D-101大孔树脂, 分离纯化, 吸附

CLC Number:

S662.1
TS255

JIN Kexu, DAI Xiaohua, GU Hongfei, SHU Jiamin, WANG Tianqi, HAI Tingyu. Study on Purification Process of Total Flavonoids from Armeniaca sibirica (L.) Lam by Macroporous Resin[J]. Xinjiang Agricultural Sciences, 2022, 59(4): 900-907.

金科旭, 戴小华, 谷虹霏, 束佳敏, 王天琪, 海婷玉. 大孔树脂纯化野山杏总黄酮工艺[J]. 新疆农业科学, 2022, 59(4): 900-907.

Figures/Tables 11

References 22

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树脂型号 Resins model	A	平均值 Average	比吸附量 Specific adsorption capacity(mg/g)	比解吸附量 Specific desorption capacity(mg/g)	解吸率 Desorption rate(%)
ADS-17	0.263 0.261 0.265	0.264	1.311	0.168	12.81
D-101	0.108 0.111 0.117	0.112	1.364	0.197	14.44
NAK-9	0.269 0.277 0.261	0.269	1.309	0.133	10.16
AB-8	0.198 0.198 0.201	0.201	1.331	0.179	13.45

树脂型号 Resins model	A	平均值 Average	比吸附量 Specific adsorption capacity(mg/g)	比解吸附量 Specific desorption capacity(mg/g)	解吸率 Desorption rate(%)
ADS-17	0.263 0.261 0.265	0.264	1.311	0.168	12.81
D-101	0.108 0.111 0.117	0.112	1.364	0.197	14.44
NAK-9	0.269 0.277 0.261	0.269	1.309	0.133	10.16
AB-8	0.198 0.198 0.201	0.201	1.331	0.179	13.45

编号 Numbering	径高比 Ratio of height to height	A	总黄酮含量 Total flavonoid content(mg/mL)
1	1:4	0.468	0.035
2	1:6	0.603	0.045
3	1:8	0.594	0.044

编号 Numbering	径高比 Ratio of height to height	A	总黄酮含量 Total flavonoid content(mg/mL)
1	1:4	0.468	0.035
2	1:6	0.603	0.045
3	1:8	0.594	0.044

流速 Flow rate (mL/min)	上样液体积 Sample volume (mL)	滤液A Filtrate A	滤液中总黄酮量 Total flavonoid content in filtrate (mg/mL)	吸附量 Adsorption capacity (mg/g)
0.5	200	0.012	0.019 4	69.98
1.0	200	0.014	0.054 8	69.95
1.5	200	0.019	0.143 4	69.86