Polyphenols from Raisins in Xinjiang: Optimization of Ultrasonic-assisted Extraction Process by Response Surface Methodology

doi:10.6048/j.issn.1001-4330.2019.04.012

Xinjiang Agricultural Sciences ›› 2019, Vol. 56 ›› Issue (4): 696-706.DOI: 10.6048/j.issn.1001-4330.2019.04.012

Polyphenols from Raisins in Xinjiang: Optimization of Ultrasonic-assisted Extraction Process by Response Surface Methodology

Xie Hui^1,2, Wang Xian³, Zhang Wen², Wu Guo-hong⁴, Wang Min², Han Shou-an², Yi Zhati·Tuerdahong⁴, Zhong Hai-xia², Pan Ming-qi², Lu Sheng-zu²,Li Jiang¹

1 College of Forestry and Horticulture Xinjiang Agricultural University, Urumqi 830052;
2 Institute of Horticultural of Xinjiang Academy of Agricultural Sciences, Urumqi 830091;
3 Research Institute of Crain Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091,China;
4 Research Institute of Grapes and Melons of Xinjiang Uygur Autonomous Region, Shanshan Xinjiang 838200, China ;
5 College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052

Received:2018-12-10 Online:2019-04-20 Published:2019-07-08
Correspondence author: Li Jiang (1958- ), male, native place: Shaodong, Hunan. Professor, Ph. D., field: Fruit Tree cultivation Physiology and Fruit Germplasm Resources, (E-mail) lijiangxj@ 163.com. Lu Sheng-zu (1962-), male, native place: Hami, Xinjiang, associate researcher, research field: Preliminary processing of fruit, (Email):1256220860@qq.com

新疆葡萄干多酚超声波辅助提取工艺响应面法优化研究

谢辉^1,2, 王仙³, 张雯², 伍国红⁴, 王敏², 韩守安², 玛尔哈巴·毛拉洪⁵, 依扎提·吐尔达洪⁴, 钟海霞², 潘明启³, 陆胜祖², 李疆¹

1.新疆农业大学林学与园艺学院,乌鲁木齐 830052;
2.新疆农业科学院园艺作物研究所,乌鲁木齐,830091;
3.新疆农业科学院粮食作物研究所,乌鲁木齐 830091;
4.新疆维吾尔自治区葡萄瓜果研究所,新疆鄯善 838200;
5.新疆农业大学食品科学与药学学院,乌鲁木齐 830052

通讯作者: 陆胜祖(1962-),男,新疆哈密人,副研究员,研究方向为果品初加工,(E-mail)1256220860@qq.com
李疆(1958-),男,湖南邵东人,教授,博士研究生导师,研究方向为果树栽培生理与种质资源,(E-mail)lijiangxj@163.com
作者简介:谢辉(1984-),男,山西永济人, 博士研究生,研究方向为葡萄栽培与加工,(E-mail)xhxjnky@163.com
基金资助:
国家自然科学基金(31460429);新疆重点研发任务专项“制干用葡萄品种的选育”(2016B01005-4);新疆自治区农牧业骨干人才培养计划;现代农业产业技术体系专项资金(CARS-29-ZP-08)

Abstract

Abstract: 【Objective】 The Box-Behnken test design combined with response surface analysis was used to optimize the extraction process of polyphenols in raisins.【Methods】The optimum range of ethanol concentration, material and liquid ratio, extraction time and extraction temperature was determined by single factor test under the condition of ultrasonic assistance. Then, response surface method was adopted to design and measure the extraction rate of total polyphenols and select the optimal conditions.【Results】The optimal conditions of extraction rate was: the ethanol concentration was 54%, material and liquid ratio was 68∶1(mL/g), extraction time was 35 min, extraction temperature was 75℃. The raisin polyphenol content in the validation test was 3.599,2 mg/g with a difference of 0.064,2 mg/g. The dark reaction time was determined to be 40 min. The content of SP522 in raisins was the highest, reaching 3.94 mg/g. The content of non-nuclear purple raisins in the main varieties was the highest, reaching 2.08 mg/g.【Conclusion】The optimized raisin polyphenol extraction process is reasonable and easy to operate

Key words: raisin; polyphenols; extraction process; response surface methodology

摘要： 【目的】研究Box-Behnken试验设计结合响应面分析法优化葡萄干中多酚的提取工艺。【方法】在超声波辅助条件下,采用单因素试验确定乙醇浓度、料液比、提取时间、提取温度的最佳范围,采用响应面法设计,测定以上4个因素3水平的多酚提取率,得到多酚超声波辅助提取的最佳工艺并进行验证。【结果】葡萄干多酚最佳提取工艺乙醇浓度为54%、料液比68∶1(mL/g)、提取时间35 min、提取温度75℃。验证试验中葡萄干多酚的含量为3.599 2 mg/g,差值为0.064 2 mg/g。暗反应时间确定为40 min。葡萄干中SP522含量最高,达到3.94 mg/g,主要品种中无核紫葡萄干含量最高,达到2.08 mg/g。【结论】优化的葡萄干多酚提取工艺易操作、合理。

关键词: 葡萄干, 多酚, 提取工艺, 响应面

CLC Number:

S663.1
S609

Xie Hui , Wang Xian , Zhang Wen , Wu Guo-hong , Wang Min , Han Shou-an , Yi Zhati·Tuerdahong , Zhong Hai-xia , Pan Ming-qi , Lu Sheng-zu ,Li Jiang. Polyphenols from Raisins in Xinjiang: Optimization of Ultrasonic-assisted Extraction Process by Response Surface Methodology[J]. Xinjiang Agricultural Sciences, 2019, 56(4): 696-706.

谢辉, 王仙, 张雯, 伍国红, 王敏, 韩守安, 玛尔哈巴·毛拉洪, 依扎提·吐尔达洪, 钟海霞, 潘明启, 陆胜祖, 李疆. 新疆葡萄干多酚超声波辅助提取工艺响应面法优化研究[J]. 新疆农业科学, 2019, 56(4): 696-706.

References 16

[1]	李忠新, 朱占江,杨莉玲,等.推进新疆葡萄干走向国际市场的技术对策研究[J].新疆农业科学, 2012, 49(6):1 103-1 109.LI Zhong-xin, ZHU Zhan-jiang, YANG Li-ling, et al. (2012). Study on the Technical Countermeasure of Promoting Xinjiang Raisin into the International Market [J]. Xinjiang Agricultural Sciences, 49(6): 1,103-1,109. (in Chinese)
[2]	谢辉, 张雯, 伍新宇,等. 新疆葡萄干生产研究现状及展望[J]. 北方园艺, 2015,(21):182-184.XIE Hui, ZHANG Wen, WU Xin-yu, et al. (2015). Present situation and prospect of Xinjiang raisin production [J]. North Horticulture, (21):182-184. (in Chinese)
[3]	谢辉,张雯,林静,等.南疆庭院无核白鸡心葡萄快速制干工艺浅析[J].农村科技,2018,(5):62-64.XIE Hui, ZHANG Wen, LIN Jing, et al. (2018). A brief analysis on the rapid dry process of seedless white heart grape in nanjiang courtyard [J]. Rural Science and Technology, (5):62-64. (in Chinese)
[4]	Morton, L. W., Croft, K. D., Puddey, I. B., & Byrne, L. (2000). Phenolic acids protect low density lipoproteins from peroxynitrite-mediated modification in vitro. Redox Report,5(2-3): 124-125.
[5]	Kountouri, A. M., Gioxari, A., Karvela, E., Kaliora, A. C., Karvelas, M., & Karathanos, V. T. (2013). Chemopreventive properties of raisins originating from greece in colon cancer cells. Food & Function, 4(3): 366-372.
[6]	Barnes, J. L., Painter, J. E., Knoll, S. E., & Carughi, A. (2011). Preliminary results using the association of official analytical chemists (AOAC) analysis method 2009.01 for fiber: raisins now contain 10% of the daily value. Journal of the American Dietetic Association, 111(9-supp-S), A47.
[7]	Camire, M. E., & Dougherty, M. P. (2003). Raisin dietary fiber composition and in vitro bile acid binding. Journal of Agricultural and Food Chemistry, 51(3): 834-837.
[8]	Parker, T.L., Wang, X.H., Pazmino, J., Engeseth, N. J. (2007). Antioxidant capacity and phenolic content of grapes, sun-dried raisins, and golden raisins and their effect on ex vivo serum antioxidant capacity. Journal of Agricultural and Food Chemistry, 55(21): 8,472-8,477.
[9]	Anderson, J. W., & Waters, A. R. (2013). Raisin consumption by humans: effects on glycemia and insulinemia and cardiovascular risk factors. Journal of Food Science, 78(S1): 11-17.
[10]	王星天, 李桂水, 程丽君,等. 响应面试验优化桑叶茶中游离氨基酸与多酚的提取工艺[J].食品科学, 2015, 36(24):83-88.WANG Xing-tian, LI Gui-shui, CHENG Li-jun, et al. (2015). Optimization of the Extraction Process of Free Amino Acids and Polyphenols from Mulberry Leaf Tea by Response Surface Methodology [J]. Food Science, 36(24):83-88. (in Chinese)
[11]	令博,王捷,吴洪斌,等. 葡萄皮渣多酚超声波辅助提取工艺响应面法优化及抗氧化活性研究[J]. 食品科学,2011,32(18):24-29.LING Bo, WANG Jie, WU Hong-bin, et al. (2011). Polyphenols from Grape Skin: Optimization of Ultrasonic-assisted Extraction Process by Response Surface Methodology and Antioxidant Activity Evaluation [J]. Food Science, 32(18):24-29. (in Chinese)
[12]	冯进, 曾晓雄, 李春阳. 响应面法优化蓝莓叶多酚提取工艺[J]. 食品科学, 2013,34(4):59-64.FENG Jin, ZENG Xiao-xiong, LI Chun-yang. (2013). Optimization of Extraction Process for Polyphenols from Blueberry Leaves by Response Surface Methodology [J]. Food Science, 34(4):59-64. (in Chinese)
[13]	邓娜, 乔沈, 高芯,等. 响应面试验优化石榴皮多酚提取工艺及石榴不同部位多酚的抗氧化活性分析[J]. 食品科学, 2016, 37(6):39-43.DENG Na, QIAO Shen, GAO Xin, et al. (2016). Optimization of Extraction Process of Polyphenols from Pomegranate Peel by Response Surface Methodology and Antioxidant Activity Analysis of Polyphenols in Different Parts of Pomegranate Fruit [J]. Food Science, 37(6):39-43. (in Chinese)
[14]	李珍,哈益明,李安,等. 响应面优化苹果皮渣多酚超声提取工艺研究[J]. 中国农业科学, 2013, 46(21):4 569-4 577.LI Zhen, HA Yi-ming, LI An, et al. (2013). Optimization of Ultrasound-Assisted ExtractionTechnology of Polyphenols from Apple Pomace by Response Surface Methodology [J]. Scientia Agricultura Sinica, 46(21):4,569-4,577. (in Chinese)
[15]	杨聪颖, 骆亚丽, 郭时印,等. 响应面优化金柑多酚的提取工艺[J]. 现代食品科技,2018,34(5):174-181.YANG Cong-ying, LUO Ya-li, GUO Shi-yin, et al. (2018). Optimization of Polyphenols from Kumquat through Response Surface Methodology [J]. Modern Food Science & Technology, 34(5):174-181. (in Chinese)
[16]	高海荣, 赵爱娟, 陈秀丽,等.从茶叶中提取茶多酚工艺的对比研究[J].中国食品添加剂,2017,(3):133-137.GAO Hai-rong, ZHAO Ai-juan, CHEN Xiu-li, et al. (2017). The comparison of three process of tea polyphenols extraction from tea [J]. China Food Additives, (3):133-137. (in Chinese)

Polyphenols from Raisins in Xinjiang: Optimization of Ultrasonic-assisted Extraction Process by Response Surface Methodology

新疆葡萄干多酚超声波辅助提取工艺响应面法优化研究

PDF

Knowledge

Abstract

Cite this article

share this article

References 16

Related Articles 15

Recommended Articles

Metrics

[1]	HU Jinge, BAI Shijian. Study on the fruit quality and raisins character of thompson seedless and Its lines [J]. Xinjiang Agricultural Sciences, 2023, 60(11): 2751-2763.
[2]	XUE Feng, PAN Xubing, WEI Dengpan, LIU Xiuhai, FAN Guoyuan, HU Jinge, ZHAO Ronghua, CAI Junshe, XIE Hui, BAI Shijian. Analysis on Cultivation Characteristics and Raisin Characteristics of Five Seedless Grape Cultivars in Turpan Region [J]. Xinjiang Agricultural Sciences, 2023, 60(1): 105-115.
[3]	ZHAO Jinghua, YANG Tingrui, ZHANG Heng, Hudan Tumaibai, MA Liang, CHEN Kaili. Optimal Selection of Water and Fertilizer for Spring Wheat under Drip Irrigation in Gravel Sandy Soil Based on Response Surface Methodology [J]. Xinjiang Agricultural Sciences, 2023, 60(1): 43-51.
[4]	GE Hui, GUO Zengxiang, ZHAO Wanqian, WANG Huizhen, WANG Pan, LIU Dong, ZHAO Xin, DU Tao. Effects of Sowing Date and Sowing Rate on Yield and Quality of Angelica sinensis Seedlings and Medicines [J]. Xinjiang Agricultural Sciences, 2022, 59(6): 1537-1544.
[5]	LIAN Weijia, XU Yanjun, Iskar Maitiniyazi, TANG Qiuju, CHEN Ya, LIU Fengjuan. Effects of Different Drying Promoters on Drying Rate and Sensory Quality of Seedless Green Grape [J]. Xinjiang Agricultural Sciences, 2022, 59(5): 1277-1283.
[6]	LIAN Weijia, CHEN Ya, HAN Chen, LI Haifeng, LIU Zhigang, XU Guixiang, LEI Jing, WU Jiuyun. Study on Optimization of Anti-browning Technology of Heat Pump Drying Seedless Green Raisins by Response Surface Methodology [J]. Xinjiang Agricultural Sciences, 2022, 59(3): 625-633.
[7]	LEI Jing, BI Jingfeng, LIAN Weijia, CHEN Ya, HAN Chen. Optimization of Pulsed Sudden Decompression Flash Drying Process for Crispy Raisin by Response Surface Methodology [J]. Xinjiang Agricultural Sciences, 2021, 58(8): 1529-1539.
[8]	LEI Jing, LIAN Weijia, CHEN Ya, HAN Chen, REN Hongsong, XIAO Li. Changes of Phenolic Substances in Seedless White and Green Raisins before and after Washing and Their Relationship with Enzymatic Browning [J]. Xinjiang Agricultural Sciences, 2021, 58(7): 1316-1322.
[9]	LEI Jing, CHEN Ya, LIAN Weijia, HAN Chen, Ayijiamali Jiapaer, REN Hongsong. Optimization of Fermentation Technology for Mulberry Fruit and Leaf Wine by Response Surface Methodology [J]. Xinjiang Agricultural Sciences, 2021, 58(6): 1113-1123.
[10]	Xie Yuqing, DAI Jinping, Chen Jin, Wang Zhifang, GuliAhmat, Yang Xinping, Zhang Huitao, Wang Xiaowu, Feng Lei. Optimizing Carbon and Nitrogen Source in Liquid Fermentation Medium of G.resinaceum LZ02 Producing Laccase with Response Surface Methodology [J]. Xinjiang Agricultural Sciences, 2020, 57(5): 869-876.
[11]	WANG Jingjing, FANG Fang, ZHOU Xiaoming, WU Xinyu, SU Min. Geographical Source Traceability of Raisins Based on Mineral Element Contents [J]. Xinjiang Agricultural Sciences, 2020, 57(1): 69-77.
[12]	WANG Xu-hui^1,2, XU Xin ³, WANG Hui², YE Kai², TU Zhen-dong², CHAO Qun-fang¹. Optimization, Purifcation and Preliminary Structural Characterization of Artemisia rupestris L. Polysaccharides [J]. Xinjiang Agricultural Sciences, 2018, 55(6): 1085-1097.
[13]	XIE Jing-long, LI Xiao-bin, ZHAO Fang, WANG Chen-chen, YANG Kai-lun. Optimization of Fermentation Medium Components for Dihydrodaidzein Producing Strain Using RSM [J]. Xinjiang Agricultural Sciences, 2018, 55(1): 143-155.
[14]	WANG Xu-hui, YE Kai, XU Xin, Shanqimike, WANG Hui, LI Guan, DENG Yu. Study on the Isolation,Identification of Salt-tolerant Cellulose-Degrading Strains and Optimization of Its Strain Ratio [J]. Xinjiang Agricultural Sciences, 2017, 54(12): 2282-2292.
[15]	XU Xin, MAO Hong-yan, YU Ming. Study on Optimization of Chickpea Resistant Starch Preparation and Its Structural Properties [J]. Xinjiang Agricultural Sciences, 2017, 54(10): 1847-1855.