Optimization, Purifcation and Preliminary Structural Characterization of Artemisia rupestris L. Polysaccharides

doi:10.6048/j.issn.1001-4330.2018.06.012

Abstract

Abstract: 【Objective】 The extraction of Artemisia rupestris L. polysaccharides (ARP) from Xinjiang was optimized by response surface method, and its separation, purification and preliminary structure analysis of ARP were carried out.【Method】Using Artemisia rupestris L. in Xinjiang as the raw material, ARP were obtained through ultrasonic-assisted method, Sevag deproteinization, DEAE cellulose and Sephadex G-100 gel filter column chromatographies. The structure of the ARP was analyzed and characterized by ultraviolet (UV) and infrared (IR) spectroscopy.【Result】A yield of ARP of 3.92%± 0.065% was obtained under optimized process condition (ultrasonic power 716 W, ratio of water to raw material 36 and ultrasonic time 25 min). The crude polysaccharides were purifed by fltration, DEAE-52 cellulose column and Sephadex G-100 size-exclusion chromatography in that order. A fraction, ARP-2 was obtained through the extraction and purifcation steps. The absorption spectra of the polysaccharides showed that the polysaccharides-related absorption peaks at 917, 1,100, 1,147, 1,332, 1,423, 1,611, 1,742, 2,939 and 3,410 cm^-1.【Conclusion】The optimized preparation process of ARP is reasonable and feasible. UV scanning showed that ARP-2 had no apparent absorption peaks of nucleic acid and protein. The characteristic absorption peak of ARP-2 containing polysaccharides was obtained by IR analysis, which has laid a foundation for the structural analysis and the pharmacological activity of ARP.

Key words: Artemisia rupestris L.; polysaccharides; ultrasonic extraction; purifcation; response surface methodology

摘要： 【目的】 采用响应面法优化超声波法提取新疆一枝蒿多糖(ARP)的工艺条件,对其进行分离纯化和结构分析,这为ARP的结构分析及药理活性研究奠定基础。【方法】以新疆一枝蒿为原料,采用超声波法提取,Sevag法除蛋白,经DEAE纤维素-52柱和Sephadex G-100凝胶色谱柱纯化得ARP。利用紫外光谱扫描、红外光谱法对其化学结构进行分析。【结果】提取ARP的优化工艺条件为超声功率716 W、液料比36∶1和超声时间25 min,在此条件下,ARP的得率为3.92%±0.065%。ARP-2的红外吸收峰出现在917、1 100、1 147、1 332、1 423、1 611、1 742、2 939和3 410 cm^-1。【结论】优化的ARP制备工艺合理、可行,紫外光谱扫描结果显示ARP-2无明显的核酸和蛋白质吸收峰,ARP-2含有多糖类物质的特征吸收峰。

关键词: 新疆一枝蒿, 多糖, 超声波法, 纯化, 响应面法

CLC Number:

:S188

WANG Xu-hui^1,2, XU Xin ³, WANG Hui², YE Kai², TU Zhen-dong², CHAO Qun-fang¹. Optimization, Purifcation and Preliminary Structural Characterization of Artemisia rupestris L. Polysaccharides[J]. Xinjiang Agricultural Sciences, 2018, 55(6): 1085-1097.

王旭辉, 徐鑫, 王卉, 叶凯, 涂振东, 晁群芳. 新疆一枝蒿多糖的提取、纯化及其结构分析[J]. 新疆农业科学, 2018, 55(6): 1085-1097.

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