Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (5): 1245-1251.DOI: 10.6048/j.issn.1001-4330.2022.05.025
• Plant Protection·Microbes·Storage and Preservation Processing • Previous Articles Next Articles
Nurziya Yarmamat1(), LUO Ying1, HAO Jingzhe1, QI Haoxuan3, PEI Longying2, SUN Chunhua4, JIA Wenjie1(), JIA Peisong1()
Received:
2021-12-05
Online:
2022-05-20
Published:
2022-06-09
Correspondence author:
JIA Wenjie, JIA Peisong
Supported by:
努尔孜亚·亚力买买提1(), 罗影1, 郝敬喆1, 祁颢萱3, 裴龙英2, 孙春花4, 贾文捷1(), 贾培松1()
通讯作者:
贾文捷,贾培松
作者简介:
努尔孜亚·亚力买买提(1977-),女,新疆人,副研究员,研究方向为食用菌资源, (E-mail) nrzy729@163.com
基金资助:
CLC Number:
Nurziya Yarmamat, LUO Ying, HAO Jingzhe, QI Haoxuan, PEI Longying, SUN Chunhua, JIA Wenjie, JIA Peisong. Regeneration and Mating Type Determination of Protoplast of Pleurotus ferulae[J]. Xinjiang Agricultural Sciences, 2022, 59(5): 1245-1251.
努尔孜亚·亚力买买提, 罗影, 郝敬喆, 祁颢萱, 裴龙英, 孙春花, 贾文捷, 贾培松. 新疆野生阿魏菇原生质体的再生与单核菌株交配型测定[J]. 新疆农业科学, 2022, 59(5): 1245-1251.
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菌种 Strain | 挑取数量 Picking number | 萌发数量 Germination number | 单核数量 Mononuclear number | 单核获得率 Mononuclear Acquisition rate (%) |
---|---|---|---|---|
PL01 | 198 | 156 | 20 | 12.8 |
PL163 | 89 | 74 | 19 | 25.68 |
PL176 | 175 | 108 | 11 | 10.18 |
Table1 The quantity of monocytic strains and the rate of monokaryogenesis
菌种 Strain | 挑取数量 Picking number | 萌发数量 Germination number | 单核数量 Mononuclear number | 单核获得率 Mononuclear Acquisition rate (%) |
---|---|---|---|---|
PL01 | 198 | 156 | 20 | 12.8 |
PL163 | 89 | 74 | 19 | 25.68 |
PL176 | 175 | 108 | 11 | 10.18 |
亲本 菌株 Parent strain | 不同交配型的阿魏菇单核菌株 Mononuclear strains of P. eryngii with different mating types |
---|---|
PL01 | 3、19、49、65、236 |
14、16、17、27、45、4、51、53、56、61、66、70、234、237、264 | |
PL176 | 22、39 |
18、23、56、71、75、79、88、146、148 | |
PL163 | 2、10、24、89 |
1、5、7、8、54、39、40、43、49、50、51、56、78、79、80 |
Table 2 The classification of mating type
亲本 菌株 Parent strain | 不同交配型的阿魏菇单核菌株 Mononuclear strains of P. eryngii with different mating types |
---|---|
PL01 | 3、19、49、65、236 |
14、16、17、27、45、4、51、53、56、61、66、70、234、237、264 | |
PL176 | 22、39 |
18、23、56、71、75、79、88、146、148 | |
PL163 | 2、10、24、89 |
1、5、7、8、54、39、40、43、49、50、51、56、78、79、80 |
菌株 Strain | 菌丝长速 Growth rate (mm/d) | 菌株 Strain | 菌丝长速 Growth rate (mm/d) |
---|---|---|---|
PL01 | 5.28±0.08a | PL163-P08 | 4.70±0.03c |
PL01-P3 | 3.57±0.1 | PL163-P10 | 2.94±0.09g |
PL01-P14 | 2.81±0.24f | PL163-P24 | 5.41±0.08b |
PL01-P16 | 5.07±0.2 | PL163-P39 | 3.42±0.16f |
PL01-P17 | 3.31±0.07e | PL163-P40 | 3.16±0.1 |
PL01-P19 | 4.74±0.17c | PL163-P43 | 3.49±0.0 |
PL01-P27 | 4.78±0.09c | PL163-P49 | 3.17±0.11h |
PL01-P45 | 4.74±0.01c | PL163-P50 | 2.02±0.04i |
PL01-P48 | 4.74±0.27c | PL163-P51 | 2.35±0.10h |
PL01-P49 | 5.26±0.1 | PL163-P54 | 4.87±0.11c |
PL01-P51 | 3.56±0.1 | PL163-P56 | 3.61±0.21f |
PL01-P53 | 3.48±0.10e | PL163-P78 | 3.28±0.10f |
PL01-P56 | 5.03±0.1 | PL163-P79 | 4.26±0.02d |
PL01-P61 | 4.94±0.3 | PL163-P80 | 2.92±0.04g |
PL01-P65 | 4.67±0.05c | PL176 | 5.99±0.19a |
PL01-P66 | 3.96±0.21d | PL176-P18 | 4.15±0.0 |
PL01-P70 | 4.85±0.14c | PL176-P22 | 4.48±0.0 |
PL01-P234 | 4.77±0.10c | PL176-P23 | 5.73±0.0 |
PL01-P236 | 4.88±0.0 | PL176-P39 | 5.08±0.15a |
PL01-P237 | 4.66±0.03c | PL176-P56 | 4.40±0.3 |
PL01-P264 | 3.89±0.34d | PL176-P71 | 4.89±0.0 |
PL163 | 6.20±0.11a | PL176-P75 | 3.34±0.3 |
PL163-P01 | 4.13±0.3 | PL176-P79 | 2.96±0.0 |
PL163-P02 | 3.26±0.29f | PL176-P88 | 4.86±0.0 |
PL163-P05 | 3.92±0.06e | PL176-P146 | 4.92±0.2 |
PL163-P07 | 6.08±0.07a | PL176-P148 | 3.93±0.2 |
Table 3 The growth speed of mononuclear strains
菌株 Strain | 菌丝长速 Growth rate (mm/d) | 菌株 Strain | 菌丝长速 Growth rate (mm/d) |
---|---|---|---|
PL01 | 5.28±0.08a | PL163-P08 | 4.70±0.03c |
PL01-P3 | 3.57±0.1 | PL163-P10 | 2.94±0.09g |
PL01-P14 | 2.81±0.24f | PL163-P24 | 5.41±0.08b |
PL01-P16 | 5.07±0.2 | PL163-P39 | 3.42±0.16f |
PL01-P17 | 3.31±0.07e | PL163-P40 | 3.16±0.1 |
PL01-P19 | 4.74±0.17c | PL163-P43 | 3.49±0.0 |
PL01-P27 | 4.78±0.09c | PL163-P49 | 3.17±0.11h |
PL01-P45 | 4.74±0.01c | PL163-P50 | 2.02±0.04i |
PL01-P48 | 4.74±0.27c | PL163-P51 | 2.35±0.10h |
PL01-P49 | 5.26±0.1 | PL163-P54 | 4.87±0.11c |
PL01-P51 | 3.56±0.1 | PL163-P56 | 3.61±0.21f |
PL01-P53 | 3.48±0.10e | PL163-P78 | 3.28±0.10f |
PL01-P56 | 5.03±0.1 | PL163-P79 | 4.26±0.02d |
PL01-P61 | 4.94±0.3 | PL163-P80 | 2.92±0.04g |
PL01-P65 | 4.67±0.05c | PL176 | 5.99±0.19a |
PL01-P66 | 3.96±0.21d | PL176-P18 | 4.15±0.0 |
PL01-P70 | 4.85±0.14c | PL176-P22 | 4.48±0.0 |
PL01-P234 | 4.77±0.10c | PL176-P23 | 5.73±0.0 |
PL01-P236 | 4.88±0.0 | PL176-P39 | 5.08±0.15a |
PL01-P237 | 4.66±0.03c | PL176-P56 | 4.40±0.3 |
PL01-P264 | 3.89±0.34d | PL176-P71 | 4.89±0.0 |
PL163 | 6.20±0.11a | PL176-P75 | 3.34±0.3 |
PL163-P01 | 4.13±0.3 | PL176-P79 | 2.96±0.0 |
PL163-P02 | 3.26±0.29f | PL176-P88 | 4.86±0.0 |
PL163-P05 | 3.92±0.06e | PL176-P146 | 4.92±0.2 |
PL163-P07 | 6.08±0.07a | PL176-P148 | 3.93±0.2 |
Fig.3 Microstructure of monokaryontic and multinucleated mycelia of 7 d Note: A: Monokaryontic mycelia ; B: Multinucleated mycelia,clamp connection by the black arrows
杂交菌株 Hybrid strain | 杂交菌株 Hybrid strain |
---|---|
PL176P23×PL01P16 | PL01P49×PL163P7 |
PL176P39×PL01P16 | PL01P49×PL163P24 |
PL176P23×PL01P49 | PL163P24×PL176P23 |
PL176P39×PL01P49 | PL163P24×PL176P39 |
PL01P16×PL163P7 | PL163P7×PL176P23 |
PL01P16×PL163P24 | PL163P7×PL176P39 |
Table 4 hybrid strain
杂交菌株 Hybrid strain | 杂交菌株 Hybrid strain |
---|---|
PL176P23×PL01P16 | PL01P49×PL163P7 |
PL176P39×PL01P16 | PL01P49×PL163P24 |
PL176P23×PL01P49 | PL163P24×PL176P23 |
PL176P39×PL01P49 | PL163P24×PL176P39 |
PL01P16×PL163P7 | PL163P7×PL176P23 |
PL01P16×PL163P24 | PL163P7×PL176P39 |
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