伊犁马MCT1基因多态性与速度性能的关联性分析

doi:10.6048/j.issn.1001-4330.2022.11.028

新疆农业科学 ›› 2022, Vol. 59 ›› Issue (11): 2815-2821.DOI: 10.6048/j.issn.1001-4330.2022.11.028

• 马运动生理与健康养殖 • 上一篇下一篇

伊犁马MCT1基因多态性与速度性能的关联性分析

吴金秋¹^,²(), 张冲¹, 孟军¹^,²^,³, 曾亚琦¹^,²^,³, 加娜尔・努尔顿⁴, 王川坤¹^,², 袁鑫鑫¹^,², 王彤亮¹^,², 姚新奎¹^,²^,³()

1.新疆农业大学动物科学学院,乌鲁木齐 830052
2.新疆农业大学马产业研究院,乌鲁木齐 830052
3.新疆马繁育与运动生理重点实验室,乌鲁木齐 830052
4.新疆伊犁哈萨克自治州昭苏马场,新疆昭苏 835000

收稿日期:2022-01-11 出版日期:2022-11-20 发布日期:2022-12-28
通信作者: 姚新奎
作者简介:吴金秋(1993-),女,新疆乌鲁木齐人,硕士研究生,研究方向为动物生产学,(E-mail)1610637765@qq.com
基金资助:
自治区重点实验室开放课题“基于血液转录组的伊犁马运动相关功能基因筛选及其功能研究”;新疆维吾尔自治区重大科技专项(2022A02007-1);自治区创新环境(人才、基地)建设专项(PT2220);新疆农业大学大学生创新项目“伊犁马MCT1基因多态性与速度性能关联性分析”(dxscx2021108)

Correlation Analysis between MCT1 Gene Polymorphismsand Speed Performance in Yili Horses

WU Jinqiu¹^,²(), ZHANG Chong¹, MENG Jun¹^,²^,³, ZENG Yaqi¹^,²^,³, Jianaer Nuerdun⁴, WANG Chuankun¹^,², YUAN Xinxin¹^,², WANG Tongliang¹^,², YAO Xinkui¹^,²^,³()

1. College of Animal Science, Xinjiang Agricultural University,Urumqi 830052 China
2. Research Institute of Horse Industry, Xinjiang Agricultural University, Urumqi 830052, China
3. Xinjiang Key Laboratory of Equine Breeding and Exercise Physiology, Urumqi 830052, China
4. Xinjiang Yili Zhaosu Stud, Zhaosu 835000, China

Received:2022-01-11 Online:2022-11-20 Published:2022-12-28
Correspondence author: YAO Xinkui
Supported by:
The open project of the key laboratory of the autonomous region "Screening and functional research of Yili horse movement-related functional genes based on blood transcriptome";Major science and technology projects of Xinjiang Uygur Autonomous Region(2022A02007-1);The Innovation Environment (Talent, Base) Construction Project of Xinjiang Uygur Autonomous Region(PT2220);"Association analysis of MCT1 gene polymorphism and speed performance in Yili horses", the innovation project of Xinjiang Agricultural University(dxscx2021108)

摘要/Abstract

摘要：

【目的】研究伊犁马MCT1基因多态性与速度性能的关联性,为专门化用途速度马的选种育种提供技术支撑。【方法】以50匹参加2 000 m速度赛的2岁伊犁马为研究对象,运用DNA直接测序法筛选伊犁马MCT1基因第2、3外显子的突变位点,分析其多态性位点以及与伊犁马2 000 m比赛成绩的关联性。【结果】在伊犁马MCT1基因第2外显子上发现一个SNP位点,在伊犁马MCT1基因第3外显子上没有发现突变位点,在g.52168582处,碱基T突变为碱基G,此突变位点为错义突变,该突变导致其编码氨基酸由苯丙氨酸变为亮氨酸,属于中度多态位点,处于Hardy-Weinberg平衡状态,共存在TT、TG、GG 3种基因型,TT基因平均速度高于GG和TG基因型平均速度,且TT基因型平均速度显著高于GG基因型平均速度(P<0.05)。【结论】在伊犁马MCT1基因第2外显子g.52168582T>G处TT基因型更有利于伊犁马2 000 m比赛速度。

关键词: 伊犁马; MCT1基因; 遗传多态性; 速度性能

Abstract:

【Objective】 The study the correlation between MCT1 gene polymorphism and speed performance in Yili horses, and provide technical support for the selection and breeding of specialized speed horses.【Methods】 50 2-year-old Yili horses participating in the 2,000 m speed race were selected as the research objects. The mutation site of exon 2 and 3 of MCT1 gene of Yili horse was screened by DNA direct sequencing method, and analyze its polymorphism loci and its association with the performance of Yili horse 2000 m race.【Results】 SNP site was found on the second exon of the Yili horse MCT1 gene, but no mutation site was found on the third exon of the Yili horse MCT1 gene. At g.52168582, the base T was mutated to the base G, and the mutation site was a missense mutation, which caused the encoded amino acid to be changed from phenylalanine. Acid changes to leucine, which belongs to moderate polymorphism site and is in a Hardy-Weinberg equilibrium state. There are three genotypes of TT, TG and GG. The correlation analysis with the 2000 m race results shows that the average speed of TT gene is higher than that of GG and GG. The average speed of TG genotype, and the average speed of TT genotype was significantly higher than that of GG genotype (P<0.05).【Conclusion】 The TT genotype at the second exon g.52168582T>G of the MCT1 gene of Yili horses is more conducive to the 2000 m race speed of Yili horses.

Key words: Yili horse; MCT1 gene; genetic polymorphism; speed performance

中图分类号:

S821.9

吴金秋, 张冲, 孟军, 曾亚琦, 加娜尔・努尔顿, 王川坤, 袁鑫鑫, 王彤亮, 姚新奎. 伊犁马MCT1基因多态性与速度性能的关联性分析[J]. 新疆农业科学, 2022, 59(11): 2815-2821.

WU Jinqiu, ZHANG Chong, MENG Jun, ZENG Yaqi, Jianaer Nuerdun, WANG Chuankun, YUAN Xinxin, WANG Tongliang, YAO Xinkui. Correlation Analysis between MCT1 Gene Polymorphismsand Speed Performance in Yili Horses[J]. Xinjiang Agricultural Sciences, 2022, 59(11): 2815-2821.

图/表 7

参考文献 26

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名称 Name	引物序列(5’→3’) Sequences(5’→3’)	退火温度 Annealing temperature	产物大小 Size (bp)
MCT1-1	F: CAATTCAGTTACATGAGAGCTAGGT R: ACATTTCACTTACCTCCACCATAC	65℃	371 bp
MCT1-2	F: ACCAAGGTGTTTTCAGCCTACC R: CCAGGGAATAGCAAATAGCCAT	65℃	342 bp

名称 Name	引物序列(5’→3’) Sequences(5’→3’)	退火温度 Annealing temperature	产物大小 Size (bp)
MCT1-1	F: CAATTCAGTTACATGAGAGCTAGGT R: ACATTTCACTTACCTCCACCATAC	65℃	371 bp
MCT1-2	F: ACCAAGGTGTTTTCAGCCTACC R: CCAGGGAATAGCAAATAGCCAT	65℃	342 bp

组分 Component	浓度 Concentration	体积 Volume(μL)
Buffer I	10×	2.0
dNTP	2.5 mM	1.6
上游引物 Forward primer	5.0 μL/M	0.6
和下游引物 Reverse primer	5.0 μL/M	0.6
HS Taq	5.0 U/μL	0.2
DNA模板 DNA template	20~50 ng/μL	2.0
ddH₂O		13.0

组分 Component	浓度 Concentration	体积 Volume(μL)
Buffer I	10×	2.0
dNTP	2.5 mM	1.6
上游引物 Forward primer	5.0 μL/M	0.6
和下游引物 Reverse primer	5.0 μL/M	0.6
HS Taq	5.0 U/μL	0.2
DNA模板 DNA template	20~50 ng/μL	2.0
ddH₂O		13.0

温度 Temperature	时间 Time	循环 Cycle
95℃预变性	5 min
95℃变性	30 s
65℃退火	30 s	10
72℃延伸	30 s
95℃变性	30 s
55℃退火	30 s	30
72℃延伸	30 s
72℃复延伸	10 min
4℃保存	∞

伊犁马MCT1基因多态性与速度性能的关联性分析

Correlation Analysis between MCT1 Gene Polymorphismsand Speed Performance in Yili Horses

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参考文献 26

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Metrics

编号 I.D.	速度 Speed (m/s)	编号 I.D.	速度 Speed (m/s)	编号 I.D.	速度 Speed (m/s)	编号 I.D.	速度 Speed (m/s)	编号I.D.	速度 Speed (m/s)
A₁	12.71	A₁₁	10.80	A₂₁	12.45	A₃₁	10.80	A₄₁	13.00
A₂	12.69	A₁₂	12.22	A₂₂	11.42	A₃₂	10.79	A₄₂	10.39
A₃	12.67	A₁₃	11.99	A₂₃	11.44	A₃₃	10.49	A₄₃	13.06
A₄	12.43	A₁₄	12.85	A₂₄	13.15	A₃₄	10.22	A₄₄	12.00
A₅	12.10	A₁₅	12.56	A₂₅	12.93	A₃₅	10.30	A₄₅	12.85
A₆	12.59	A₁₆	10.49	A₂₆	11.60	A₃₆	12.34	A₄₆	13.22
A₇	11.27	A₁₇	12.00	A₂₇	10.24	A₃₇	12.12	A₄₇	11.93
A₈	10.52	A₁₈	12.62	A₂₈	10.23	A₃₈	11.85	A₄₈	12.13
A₉	12.17	A₁₉	11.34	A₂₉	10.33	A₃₉	12.23	A₄₉	12.55
A₁₀	11.81	A₂₀	12.49	A₃₀	10.12	A₄₀	12.99	A₅₀	13.63

	β折叠(％) Beta turn	α螺旋 Alpha helix (％)	无规卷曲度 Random coil (％)	伸展度 Extended strand (％)
突变前 Before mutatin	10.48	27.54	28.25	33.73
突变后 After mutatin	10.79	28.57	27.30	33.33

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