新疆农业科学 ›› 2023, Vol. 60 ›› Issue (8): 1966-1974.DOI: 10.6048/j.issn.1001-4330.2023.08.018
马新超1(), 轩正英1(), 闵昊哲1, 齐志文1, 成宏宇1, 谭占明1, 王旭峰2
收稿日期:
2022-11-09
出版日期:
2023-08-20
发布日期:
2023-08-14
通信作者:
轩正英(1979-),女,辽宁朝阳人,教授,硕士生导师,研究方向为特色蔬菜种质资源与栽培生理,(E-mail)xzyzky@163.com作者简介:
马新超(1998-),男,河南南阳人,硕士研究生,研究方向为设施农业,(E-mail)mxczky@163.com
基金资助:
MA Xinchao1(), XUAN Zhengying1(), MIN Haozhe1, QI Zhiwen1, CHENG Hongyu1, TAN Zhanming1, WANG Xufeng2
Received:
2022-11-09
Online:
2023-08-20
Published:
2023-08-14
Correspondence author:
XUAN Zhengying (1979-), female, born in Chaoyang, Liaoning Province, professor, master tutor, research direction is vegetable germplasm resources and cultivation physiology of southern Xinjiang, (E-mail)xzyzky@163.comSupported by:
摘要:
【目的】研究水氮耦合对沙培黄瓜光合日变化及叶绿素荧光参数的影响,为沙培黄瓜生产提供科学的理论依据。【方法】以优胜美水果黄瓜为试材,采用2次饱和D-最优设计进行沙培黄瓜水氮耦合的田间试验,共设7个处理,在结果盛期测定黄瓜叶片光合日变化特征参数、叶绿素荧光参数、叶绿素含量和RuBP羧化酶活性。【结果】各处理的净光合速率、气孔导度及蒸腾速率日变化均呈现为单峰曲线,瞬时水分利用效率日变化总体上呈现为“升-降-升”的“N”型曲线。在适宜的水分供应下增施氮肥能够有效提高作物净光合速率,气孔导度及蒸腾速率均随着灌水水平和施氮量的增加呈现出开口朝下的抛物线趋势,瞬时水分利用效率随着灌水水平的增加而增加,但过高的灌水水平会降低瞬时水分利用效率。施氮量是影响叶片总叶绿素含量和叶绿素荧光参数的关键因子,施氮量与总叶绿素含量和PSⅡ活性等叶绿素荧光参数之间呈正相关,总叶绿素含量和叶绿素荧光参数随着灌水水平的增加大体上呈现出开口朝下的抛物线趋势;灌水水平和施氮量与RuBP羧化酶活性之间均呈开口朝下的抛物线趋势。水氮耦合下各处理光合特性的优劣排序为T4>T5>T6>T7>T3>T2>T1。【结论】T4为光合特性表现最优处理,水氮耦合方案是灌水水平为86.10%,施氮量为893.49 kg/hm2。
中图分类号:
马新超, 轩正英, 闵昊哲, 齐志文, 成宏宇, 谭占明, 王旭峰. 水氮耦合对沙培黄瓜光合日变化及叶绿素荧光参数的影响[J]. 新疆农业科学, 2023, 60(8): 1966-1974.
MA Xinchao, XUAN Zhengying, MIN Haozhe, QI Zhiwen, CHENG Hongyu, TAN Zhanming, WANG Xufeng. Effects of water and nitrogen coupling on diurnal changes of photosynthesis and chlorophyll fluorescence parameters of cucumber in sandy culture[J]. Xinjiang Agricultural Sciences, 2023, 60(8): 1966-1974.
处理 Treat- ments | 码值方案 Code value scheme | 实际值 Actual value | ||
---|---|---|---|---|
灌水水平 Irrigation level | 施氮量 N application | 灌水水平 Irrigation level(%) | 施氮量 N application (kg/hm2) | |
T1 | -1 | -1 | 65 | 150 |
T2 | 1 | -1 | 100 | 150 |
T3 | -1 | 1 | 65 | 1 250 |
T4 | -0.131 5 | -0.131 5 | 80.20 | 623 |
T5 | 0.394 4 | 1 | 89.40 | 1 250 |
T6 | 1 | 0.394 4 | 100 | 917 |
T7 | 1 | 1 | 100 | 1 250 |
表1 黄瓜水氮耦合试验设计
Tab.1 Design scheme of cucumber water nitrogen coupling test
处理 Treat- ments | 码值方案 Code value scheme | 实际值 Actual value | ||
---|---|---|---|---|
灌水水平 Irrigation level | 施氮量 N application | 灌水水平 Irrigation level(%) | 施氮量 N application (kg/hm2) | |
T1 | -1 | -1 | 65 | 150 |
T2 | 1 | -1 | 100 | 150 |
T3 | -1 | 1 | 65 | 1 250 |
T4 | -0.131 5 | -0.131 5 | 80.20 | 623 |
T5 | 0.394 4 | 1 | 89.40 | 1 250 |
T6 | 1 | 0.394 4 | 100 | 917 |
T7 | 1 | 1 | 100 | 1 250 |
处理 Treatments | PSⅡ实际光化学量子产量 PSⅡ Actual Photochemical Quantum Yield (ΦPSⅡ) | PSⅡ最大光化学效率 PSⅡ maximum photochemical efficiency (Fv/Fm) | 光化学猝灭系数 Photochemical quenching coefficient (qP) | 非光化学猝灭系数 Non-photochemical quenching coefficient (NPQ) |
---|---|---|---|---|
T1 | 0.30±0.02d | 0.75±0.03d | 0.87±0.04c | 0.24±0.04a |
T2 | 0.30±0.01d | 0.71±0.02e | 0.85±0.04c | 0.22±0.0 |
T3 | 0.36±0.02bc | 0.84±0.01abc | 0.90±0.03b | 0.22±0.03ab |
T4 | 0.37±0.01b | 0.84±0.01ab | 0.93±0.02ab | 0.19±0.03b |
T5 | 0.42±0.01a | 0.85±0.02a | 0.94±0.02a | 0.19±0.01b |
T6 | 0.35±0.02c | 0.83±0.01bc | 0.93±0.01ab | 0.21±0.02ab |
T7 | 0.36±0.02bc | 0.81±0.02c | 0.93±0.02ab | 0.24±0.04a |
表2 水氮耦合下沙培黄瓜叶绿素荧光参数变化
Tab.2 Effect of water and nitrogen coupling on chlorophyll fluorescence parameters of cucumber in sand culture
处理 Treatments | PSⅡ实际光化学量子产量 PSⅡ Actual Photochemical Quantum Yield (ΦPSⅡ) | PSⅡ最大光化学效率 PSⅡ maximum photochemical efficiency (Fv/Fm) | 光化学猝灭系数 Photochemical quenching coefficient (qP) | 非光化学猝灭系数 Non-photochemical quenching coefficient (NPQ) |
---|---|---|---|---|
T1 | 0.30±0.02d | 0.75±0.03d | 0.87±0.04c | 0.24±0.04a |
T2 | 0.30±0.01d | 0.71±0.02e | 0.85±0.04c | 0.22±0.0 |
T3 | 0.36±0.02bc | 0.84±0.01abc | 0.90±0.03b | 0.22±0.03ab |
T4 | 0.37±0.01b | 0.84±0.01ab | 0.93±0.02ab | 0.19±0.03b |
T5 | 0.42±0.01a | 0.85±0.02a | 0.94±0.02a | 0.19±0.01b |
T6 | 0.35±0.02c | 0.83±0.01bc | 0.93±0.01ab | 0.21±0.02ab |
T7 | 0.36±0.02bc | 0.81±0.02c | 0.93±0.02ab | 0.24±0.04a |
处理 Treat- ments | 正理想解 距离D+ Positive ideal solution distance | 负理想解 距离D- Negative ideal solution distance | 相对接近度 Ci Relative proximity | 排序 Sorts |
---|---|---|---|---|
T1 | 0.384 | 0.052 | 0.12 | 7 |
T2 | 0.293 | 0.162 | 0.357 | 6 |
T3 | 0.257 | 0.175 | 0.405 | 5 |
T4 | 0.16 | 0.298 | 0.651 | 1 |
T5 | 0.163 | 0.288 | 0.639 | 2 |
T6 | 0.183 | 0.268 | 0.594 | 3 |
T7 | 0.227 | 0.321 | 0.586 | 4 |
表3 光合生理指标的综合评价
Tab.3 Comprehensive evaluation of photosynthetic physiological index
处理 Treat- ments | 正理想解 距离D+ Positive ideal solution distance | 负理想解 距离D- Negative ideal solution distance | 相对接近度 Ci Relative proximity | 排序 Sorts |
---|---|---|---|---|
T1 | 0.384 | 0.052 | 0.12 | 7 |
T2 | 0.293 | 0.162 | 0.357 | 6 |
T3 | 0.257 | 0.175 | 0.405 | 5 |
T4 | 0.16 | 0.298 | 0.651 | 1 |
T5 | 0.163 | 0.288 | 0.639 | 2 |
T6 | 0.183 | 0.268 | 0.594 | 3 |
T7 | 0.227 | 0.321 | 0.586 | 4 |
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