解淀粉芽孢杆菌B1619对加工番茄幼苗促生及培育壮苗的影响

doi:10.6048/j.issn.1001-4330.2022.05.026

新疆农业科学 ›› 2022, Vol. 59 ›› Issue (5): 1252-1259.DOI: 10.6048/j.issn.1001-4330.2022.05.026

• 植物保护·微生物·贮藏保鲜加工 • 上一篇下一篇

解淀粉芽孢杆菌B1619对加工番茄幼苗促生及培育壮苗的影响

汪飞¹(), 邱光²(), 罗文芳¹, 孙晓军¹, 杨洪梅³, 刘成军³, 许建军¹, 何伟¹

1.新疆农业科学院植物保护研究所/农业部西北荒漠作物有害生物综合治理重点实验室,乌鲁木齐 830091
2.江苏省农业科学院种质资源与生物技术研究所,南京 210014
3.中粮屯河焉耆番茄制品有限公司,新疆焉耆 841100

收稿日期:2021-11-03 出版日期:2022-05-20 发布日期:2022-06-09
通信作者: 邱光
作者简介:汪飞(1970-),男,湖北人,副研究员,研究方向为农作物病虫害防治,(E-mail) 1026028505@qq.com
基金资助:
自治区科技支疆项目计划(指令性)项目(2019E0257)

Study on Application Technology of Bacillus amyloliquefaciens B1619 in Promoting Healthy Growth of Tomato Seedlings

WANG Fei¹(), QIU Guang²(), LUO Wenfang¹, SUN Xiaojun¹, YANG Hongmei³, LIU Chengjun³, XU Jianjun¹, HE Wei¹

1. Key Laboratory of Intergraded Management of Harmful Crop Vermin of China Northwestern Oasis, Ministry of Agriculture and Rural Affairs of the P.R.C. /Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China
2. Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences,Nanjin 210014,China
3. COFCO Tunhe Yanqi Tomato Products Co. Led, Yanqi Xinjiang 841100,China

Received:2021-11-03 Online:2022-05-20 Published:2022-06-09
Correspondence author: QIU Guang
Supported by:
S & T Assisting Xinjiang Program Project of Xinjiang Uygur Autonomous Region(2019E0257)

摘要/Abstract

摘要：

【目的】研究解淀粉芽孢杆菌Bacillus amyloliquefaciens B1619对加工番茄幼苗促生和苗期病害防控的综合作用,为培育壮苗,提供绿色的管理技术。【方法】采用室内穴盘育苗试验,分别用基质拌药和苗期喷雾法,研究解淀粉芽孢杆菌B1619对加工番茄幼苗生长的促进作用,以及对苗期病害的预防作用,研究其促生和防病的效果。【结果】各处理的出苗率达到稳定状态,呈现出现先抑制后促进的生长态势。1∶150出苗率最好、1∶100次之,1∶200出苗率较差,拌土法比喷雾法对番茄株高的促生作用更强。以1∶150拌土对番茄茎粗的促进作用最强。不同拌土量处理的叶绿素含量与对照之间具有极显著性差异,叶绿素含量比对照增加7.68%~10.20%,拌土法对幼苗猝倒病具有较强的预防作用,1∶150和1∶200拌土发病率只有0.00~0.08%。【结论】解淀粉芽孢杆菌B1619能够改善加工番茄育苗穴盘的土壤生态环境,施用后土壤蓬松,对番茄根系和幼苗的生物量比对照有显著性增加;叶片颜色较深,叶绿素含量增加。能够提高番茄免疫力,增强抗病性,对加工番茄幼苗猝倒病具有较强的预防作用。

关键词: 解淀粉芽孢杆菌B1619; 加工番茄; 使用方法; 促生作用; 病害控制

Abstract:

【Objective】 To study the comprehensive effects of Bacillus amyloliquefaciens B1619 on growth promotion and disease prevention of processed tomato seedlings in the hope of providing a set of healthy, ecological and green management technology for the cultivation of robust seedlings and for the promotion of healthy development of processed tomato industry in Xinjiang. 【Method】 The effects of Bacillus amylolitica B1619 to growth of tomato seedlings and the prevention of seedling diseases were studied with matrix mixed medicine and spray method through laboratory experiment of hole dish seedling at seedling stage. 【Result】 The Bacillus amylolitica B1619 improves seedling emergence rate, promote growth, prevent and control damping off, its also beneficial to cultivate healthy seedlings, improve immunity, and reduce soil-borne diseases of tomato seedlings.1. Bacillus amylolitica B1619 improved the soil ecological environment of tomato seedling hole disk. The soil was loose, biomass of tomato root and seedling was significantly increased after application of bacillus compared with the control. The leaves were darker and the chlorophyll content increased. 2.Its improved immunity of tomato, enhanced disease resistance, and processed tomato seedlings had a strong role in the prevention of cataplexy. 【Conclusion】 B.amylum B1619 can improve the ecological environment of the biomass of tomato roots and seedlings; the leaf color deepens and the chlorophyll content increases. It can improve tomato immunity, enhance disease resistance, and have a strong preventive effect on cataplexy in processed tomato seedlings.

Key words: Bacillus amyloliquefaciens B1619; processed tomato; usage method; promotional role; disease control

中图分类号:

S188

汪飞, 邱光, 罗文芳, 孙晓军, 杨洪梅, 刘成军, 许建军, 何伟. 解淀粉芽孢杆菌B1619对加工番茄幼苗促生及培育壮苗的影响[J]. 新疆农业科学, 2022, 59(5): 1252-1259.

WANG Fei, QIU Guang, LUO Wenfang, SUN Xiaojun, YANG Hongmei, LIU Chengjun, XU Jianjun, HE Wei. Study on Application Technology of Bacillus amyloliquefaciens B1619 in Promoting Healthy Growth of Tomato Seedlings[J]. Xinjiang Agricultural Sciences, 2022, 59(5): 1252-1259.

图/表 9

参考文献 20

[1]	张萍, 赵建华, 鞠召彬, 等. 放线菌肥对新疆加工番茄促生、防病增产及列当的防控效果[J]. 中国蔬菜, 2019,(2):49-52.
	ZHANG Ping, ZHAO Jianhua, JU Zhaobin, et al. Effect of Actinomycete Fertilizer on Growth Promotion,Disease Prevention,Yield Increase and Broomrape Control of Processing Tomato in Xinjiang[J]. China Vegetables, 2019,(2):49-52.
[2]	李凯, 谭丹. 新疆番茄产业国际竞争力分析[J]. 区域经济, 2018, (35):65-68.
	LI Kai, TAN Dan. Analysis on the International Competitiveness of Tomato Industry in Xinjiang[J]. District Economy, 2018(35):65-68.
[3]	崔元玗, 杨华, 孙晓军, 等. 新疆加工番茄主要病害发生[J]. 新疆农业科学, 2004, 41(3):160-163.
	CUI Yuanyu, YANG Hua, SUN Xiaojun, et al. Occurrence and Damage of Important Diseases of Processing Tomato in Xinjiang[J]. Xinjiang Agricultural Sciences, 2004, 41(3):160-163.
[4]	Lugtenberg B, Kamilova F. Plant - growth - promoting rhizobacteria[J]. Annual Review of Microbiology, 2009, 63:541-556 DOI PMID
[5]	郑爱萍, 李平. 微生物生物防治存在的问题及发展方向[J]. 中国农学通报, 2000, 16(6):28-30.
	ZHENG Aiping, LI Ping. Microbial biological control problems and development direction[J]. Chinese Agriculture Science Bulletin, 2000, 16(6):28-30.
[6]	邱光, 张新凤, 李建伟, 等. 解淀粉芽孢杆菌B1619对草莓保苗促生效果[J]. 江苏农业科学, 2019, 47(4):98-100.
	QIU Guang, ZHANG Xinfeng, LI Jianwei, et al. Effect of bacillus amyloliquefaciens B1619 on growth of strawberry seedlings[J]. Jiangsu Agricultural Sciences, 2019, 47(4):98-100.
[7]	邱光, 李建伟, 张新凤, 等. 有益芽孢杆菌类生物药剂是解决土壤问题的根本出路[J]. 农药市场信息, 2018,(13):49-55.
	QIU Guang, LI Jianwei, ZHANG Xinfeng, et al. Beneficial bacillus biological agents are the fundamental way to solve soil problems[J]. Pesticide Market News, 2018,(13):49-55.
[8]	王立伟, 王明友. 生物菌肥对番茄连作土壤质量及根结线虫病的影响[J]. 河南农业科学, 2014, 43(4):51-55.
	WANG Liwei, WANG Mingyou. Effects of microbial manure on soil quality and root knot nematode disease under tomato continuous cropping[J]. Journal of Henan Agricultural Sciences, 2014, 43(4):51-55.
[9]	王玉, 卞中华, 胡晓辉, 等. 放线菌剂对连作番茄生长及叶片PPO活性的影响[J]. 西北农林科技大学学报(自然科学版), 2012, 40(5):99-103.
	WANG Yu, BIAN Zhonghua, HU Xiaohui, et al. Effect of actinomycets on potato growth and PPO Activity during seedling stage in continuously cropped soils[J]. Journal of Northwest A&F University (Natural Science Ed.), 2012, 40(5):99-103.
[10]	柴阿丽, 吴兰平, 迟庆勇, 等. 加工番茄病害主要类型及防治建议[J]. 中国蔬菜, 2014,(10):62-64.
	CHAI Ali, WU Lanping, CHI Qingyong, et al. Main types and control suggestions of processing tomato diseases[J]. China Vegetables, 2014,(10):62-64.
[11]	何伟, 许建军, 杨华, 等. 复合微生物菌剂“宁盾”在新疆加工番茄田间应用效果[J]. 新疆农业科学, 2018, 55(11) : 2042-2050. DOI
	HE Wei, XU Jianjun, YANG Hua, et al. Study of the field application effect of compound microbial inoculum "Ningdun" on processing tomatoes in Xinjiang[J]. Xinjiang Agricultural Sciences, 2018, 55(11) : 2042-2050. DOI
[12]	Omar I, O’neill T M, Rossall S. Biological control of Fusarium crown and root rot of tomato with antagonistic bacteria and integrated control when combined with the fungicide carbendazim[J]. Plant Pathology, 2006, 55(1):92-99. DOI URL
[13]	郝晓娟, 刘波, 谢关林, 等. 短短芽孢杆菌JK-2菌株对番茄枯萎病的抑菌作用及其小区防效[J]. 中国生物防治, 2007, 23(3): 233-236.
	HAO Xiaojuan, LIU Bo, XIE Guanlin, et al. Inhibition of Brevibacillus brevis JK-2 strain against the pathogen of tomato fusarium wilt[J]. Chinese Journal of Biological Control, 2007, 23(3): 233-236.
[14]	王璐瑶. 生防解淀粉芽孢杆菌B1619生物学特性、诱导抗病性、田间应用技术的研究[D]. 南京: 南京农业大学, 2017.
	WANG Luyao. The Biological Characteristics Characteristics, Induced Resistance, Application Technologies of Biocotrol Bacterium B 1619[D]. Nanjing: Nanjing Agricultural University, 2017.
[15]	王璐瑶, 蒋盼盼, 甘颖, 等. 解淀粉芽孢杆菌B1619诱导番茄抗病酶活性提高和抗病相关基因的表达上调[J]. 中国生物防治学报, 2017, 33(2):234-240.
	WANG Luyao, JIANG Panpan, GAN Ying, et al. Increase of Defense Enzyme Activity and Up-expression of Defense Gene in Tomato Induced by Bacillus amyloliquefaciens B1619[J]. Chinese Journal of Biological Control, 2017, 33(2):234-240.
[16]	杨晓云, 陈志谊, 蒋盼盼, 等. 解淀粉芽孢杆菌B1619对番茄的促生作用[J]. 中国生物防治学报, 2016, 33(3):349-356.
	YANG Xiaoyun, CHEN Zhiyi, JIANG Panpan, et al. Growth-promotion effect of Bacillus amyloliquefaciens B1619 on tomato plant[J]. Chinese Journal of Biological Control, 2016, 33(3):349-356.
[17]	詹发强, 侯敏, 杨蓉, 等. 一株番茄枯萎病生防菌的鉴定、定殖与盆栽防效研究[J]. 新疆农业科学, 2013, 50(7): 1277-1287.
	ZHAN Faqiang, HOU Min, YANG Rong, et al. Identification, colonization and control effect of an antagonistic bacterium against Fusarium wilt tomato[J]. Xinjiang Agricultural Sciences, 2013, 50(7): 1277-1287.
[18]	向亚萍, 周华飞, 刘永锋, 等. 解淀粉芽孢杆菌B1619脂肽类抗生素的分离鉴定及其对番茄枯萎病菌的抑制作用[J]. 中国农业科学, 2016, 49(15):2935-2944.
	XIANG Yaping, ZHOU Huafei, LIU Yongfeng, et al. Isolation and Identification of Lipopeptide Antibiotics Produced by Bacillus amyloliquefaciens B1619 and the Inhibition of the Lipopeptide Antibiotics to Fusarium oxysporum f. sp. Lycopersici[J]. Scientia Agricultura Sinica, 2016, 49(15):2935-2944.
[19]	王夏雯, 孟佳丽, 刘永锋, 等. 淀粉芽孢杆菌 B1619对连作西瓜生长及枯萎病发生的影响[J]. 福建农业学报, 2019, 34(11):1302-1308.
	WANG Xiawen, MENG Jiali, LIU Yongfeng, et al. Effects of Bacillus amyloliquefaciens B1619 on Growth and Fusarium Wilt of Continuous Cropping Watermelon[J]. Fujian Journal of Agricultural Sciences, 2019, 34(11):1302-1308.
[20]	乔俊卿, 刘邮洲, 夏彦飞, 等. 生防菌B1619在番茄根部的定植及对根际微生态的影响[J]. 植物保护学报, 2013, 40(6):507-511.
	QIAO Junqin, LIU Youzhou, XIA Yanfei, et al. Root colonization by Bacillus amyloliquefaciens B1619 and its impact on the microbial community of tomato rhizosphere[J]. Acta Phytophylacica Sinica, 2013, 40(6):507-511.

处理 Treat ment	3 d出苗率 Seedling emergence rate after 3 d(%)	6 d出苗势 Seedling emergence rate after 6 d(%)	比CK±(%)
1∶100	88.20^ab	92.73±0.89^ab	1.37
1∶150	90.50^a	95.70 ±0.59^a	4.61
1∶200	75.55^b	89.92 ±1.65^b	- 1.71
0	87.73^ab	91.48 ± 1.13^ab	—

处理 Treat ment	3 d出苗率 Seedling emergence rate after 3 d(%)	6 d出苗势 Seedling emergence rate after 6 d(%)	比CK±(%)
1∶100	88.20^ab	92.73±0.89^ab	1.37
1∶150	90.50^a	95.70 ±0.59^a	4.61
1∶200	75.55^b	89.92 ±1.65^b	- 1.71
0	87.73^ab	91.48 ± 1.13^ab	—

处理 Treat ments	根鲜重 Root fresh weight(g)	比CK ±(%)	茎叶鲜重 Leaf and stem fresh weight (g)	比CK ±(%)	整株鲜重 Fresh weight (g)	比CK ±(%)	整株干重 Dry weight (g)	比CK ±(%)
1∶100	0.100^Bb	16.57	1.43^b	2.09	1.55^b	0.87	0.25^a	7.85
1∶150	0.124^Aa	43.60	1.68^a	20.02	1.73^a	12.66	0.25^a	7.9
1∶200	0.128^Aa	48.55	1.61^a	15.18	1.68^a	9.4	0.26^a	11.73
对照Control	0.086^Bb	/	1.40^b	/	1.54^b	/	0.23^a	/

处理 Treat ments	根鲜重 Root fresh weight(g)	比CK ±(%)	茎叶鲜重 Leaf and stem fresh weight (g)	比CK ±(%)	整株鲜重 Fresh weight (g)	比CK ±(%)	整株干重 Dry weight (g)	比CK ±(%)
1∶100	0.100^Bb	16.57	1.43^b	2.09	1.55^b	0.87	0.25^a	7.85
1∶150	0.124^Aa	43.60	1.68^a	20.02	1.73^a	12.66	0.25^a	7.9
1∶200	0.128^Aa	48.55	1.61^a	15.18	1.68^a	9.4	0.26^a	11.73
对照Control	0.086^Bb	/	1.40^b	/	1.54^b	/	0.23^a	/

处理方式 Treatments		叶绿素 (Spad) chlorophyll	比CK± (%)	处理方式 Treatments		叶绿素 (Spad) chlorophyll	比CK± (%)
用量 Dosage	方法 Methods	叶绿素 (Spad) chlorophyll	比CK± (%)	用量 Dosage	方法 Methods	叶绿素 (Spad) chlorophyll	比CK± (%)
20倍	喷雾	37.92^a	-0.71	1:100	拌土	56.83^Aa	10.20
30倍	喷雾	37.67^a	-1.36	1:150	拌土	56.00^Aa	8.60
40倍	喷雾	39.44^a	3.27	1:200	拌土	55.53^Aa	7.68
CK		38.19^a	/	对照		51.57^Bb

解淀粉芽孢杆菌B1619对加工番茄幼苗促生及培育壮苗的影响

Study on Application Technology of Bacillus amyloliquefaciens B1619 in Promoting Healthy Growth of Tomato Seedlings

在线阅读

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 20

相关文章 15

编辑推荐

Metrics

处理Treatments		调查株数 (株) Survey number	发病株数 (株) Diseased number	发病率 (%) Rate	防控效果 (%) Prevention effects
药剂 Chemicals	施药方式 Methods	调查株数 (株) Survey number	发病株数 (株) Diseased number	发病率 (%) Rate	防控效果 (%) Prevention effects
B1619	1∶100拌土	1 280	6	0.47^A	97.86^A
	1∶150拌土	1 280	1	0.08^A	99.64^A
	1∶200拌土	1 280	0	0.00^A	100.00^A
精甲霜锰锌	600倍液喷雾	1 280	21	1.64^B	92.53^B
空白对照 Control		1 280	281	21.95^C	-

[1]	蒲敏, 阮向阳, 肖乐乐, 索常凯, 陈国永, 冶军, 高波. 枸溶性钙镁肥对加工番茄钙、镁吸收及品质的影响[J]. 新疆农业科学, 2023, 60(8): 1987-1995.
[2]	杜红艳, 庞胜群, 马海翔, 吉雪花. 加工番茄早熟突变体农艺性状相关性及通径分析[J]. 新疆农业科学, 2023, 60(4): 943-950.
[3]	芦大伟, 哈丽哈什·依巴提, 李青军. 磷肥用量对滴灌加工番茄产量、品质及磷肥利用率的影响[J]. 新疆农业科学, 2023, 60(1): 185-191.
[4]	林青, 史应武, 王娜, 华兰兰, 杨红梅, 楚敏, 曾军, 高雁, 霍向东. 加工番茄系统抗性诱导促生菌的筛选鉴定及其促生防病效果[J]. 新疆农业科学, 2022, 59(6): 1466-1474.
[5]	何伟, 罗文芳, 周军辉, 许建军, 孙晓军. 贝莱斯芽孢杆菌JTB8-2对加工番茄促生作用及其安全性评价[J]. 新疆农业科学, 2022, 59(5): 1260-1269.
[6]	郭慧静, 李自芹, 李冀新, 赵志永, 宋方圆. 加工番茄全自动耐压测定仪的设计与性能评价[J]. 新疆农业科学, 2022, 59(10): 2495-2501.
[7]	王海琪, 庞胜群, 杜红艳, 吉雪花, 马海翔, 张小燕. 加工番茄亲代与子代生长期内源激素含量与植株生长相关性分析[J]. 新疆农业科学, 2021, 58(1): 40-48.
[8]	宋方圆, 赵志永, 李冀新. 筛选品质优良的新疆主栽加工番茄品种[J]. 新疆农业科学, 2020, 57(7): 1267-1275.
[9]	景博, 牛宁, 张文龙, 刁明. 不同施氮量对加工番茄生长及土壤氮素平衡的影响[J]. 新疆农业科学, 2020, 57(10): 1830-1838.
[10]	杨涛, 唐亚萍, 张国儒, 李晓琴, 帕提古丽, 王柏柯, 李宁, 王娟, 余庆辉, 杨生保. 基于加工用番茄果实及果肉组织主要性状的相关性分析[J]. 新疆农业科学, 2019, 56(5): 797-807.
[11]	哈丽哈什·依巴提, 张炎, 李青军. 新型尿素对加工番茄的产量和氮素利用率的影响[J]. 新疆农业科学, 2019, 56(2): 278-286.
[12]	张晓宏,郭刚,王著龙,周小云. 外源5-氨基乙酰丙酸对加工番茄叶光合色素及果实产量品质的影响[J]. 新疆农业科学, 2018, 55(8): 1436-1443.
[13]	何伟, 许建军, 杨华, 孙晓军. 加工番茄田列当致病菌的筛选与鉴定[J]. 新疆农业科学, 2018, 55(6): 1124-1132.
[14]	付紫梅,袁伦,邵冬南,郝小军,崔百明,向本春. 靶向加工番茄elF4E1的CRISPR/Cas9载体有效性验证[J]. 新疆农业科学, 2018, 55(2): 230-237.
[15]	单淑玲, 庞胜群, 郭晓珊, 张国儒. 加工番茄小孢子细胞学发育时期与花器形态相关性研究[J]. 新疆农业科学, 2018, 55(11): 2028-2034.