不同浓度GSNO对盐胁迫下番茄幼苗生长的影响

doi:10.6048/j.issn.1001-4330.2021.04.005

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (4): 625-633.DOI: 10.6048/j.issn.1001-4330.2021.04.005

• 作物遗传育种·种质资源·分子遗传学·耕作栽培 • 上一篇下一篇

不同浓度GSNO对盐胁迫下番茄幼苗生长的影响

孙艳芸, 朱普生, 刁明, 崔金霞, 徐巍, 刘慧英

石河子大学农学院园艺系/特色果蔬栽培生理与种质资源利用兵团重点实验室,新疆石河子 832000

收稿日期:2020-04-13 出版日期:2021-04-20 发布日期:2021-04-25
通信作者: 刘慧英(1970-),女,新疆人,教授,研究方向为蔬菜抗逆生理、设施园艺,(E-mail) hyliuok@aliyun.com
作者简介:孙艳芸(1994-),女,新疆人,硕士研究生,研究方向为设施园艺,(E-mail)1498147172@qq.com
基金资助:
国家自然科学基金(31860550);兵团专利运用与产业化计划项目(2016CD003-05)

Effects of Different Concentrations of S-nitrosoglutathione (GSNO) on the Growth of Tomato Seedlings under Salt Stress

SUN Yanyun, ZHU Pusheng, DIAO Ming, CUI Jinxia, XU Wei, LIU Huiying

Department of Horticulture, College of Agronomy, Shihezi University/Key Laboratory of Cultivation Physiology and Germplasm Resources Utilization of Characteristics Fruits and Vegetables of Xinjiang Production and Construction Corps,Shihezi University,Shihezi Xinjiang 832003,China

Received:2020-04-13 Online:2021-04-20 Published:2021-04-25
Correspondence author: LIU Huiying (1970 -), female, native place:Xinjiang, Professor, research field: for Vege Table Stress Physiology, facility horticulture research, (E-mail)hyliuok@aliyun.com
Supported by:
National Natural Science Foundation of China(31860550)and Patent Application and Industrialization Project of XPCC (2016 CD003-05)

摘要/Abstract

摘要： 【目的】 研究不同浓度S-亚硝基谷胱甘肽(GSNO)对盐胁迫下番茄幼苗生长的影响,筛选出提高番茄耐盐性的适宜施用浓度。【方法】 选用番茄品种中蔬4号。采用营养液栽培法。NaCl(100 mmol/L)胁迫下,番茄幼苗叶面分别喷施不同浓度的GSNO(0、0.1、0.2、0.3、0.4、0.5、0.6 mmol/L)。测定分析不同浓度外源GSNO对盐胁迫下番茄幼苗生长及根系活力、叶绿素及丙二醛(MDA)含量、电解质渗透率和脯氨酸(Pro)含量的影响。【结果】 (1)NaCl胁迫显著降低了番茄幼苗的株高、茎粗、地上部和地下部干重、鲜重、根系活力、叶绿素a、叶绿素b和叶绿素总量;显著提高了番茄幼苗的丙二醛含量和脯氨酸含量;对电解质渗透率无显著影响;(2)外源施用0.1~0.3 mmol/L GSNO可通过不同程度地提高叶绿素含量、根系活力和脯氨酸含量,降低膜脂过氧化和膜完整性的破坏程度而有效缓解盐胁迫对番茄幼苗生长的抑制作用和提高盐适应性,而实验施用的高浓度(0.5~0.6 mmol/L)GSNO的施用虽对盐胁迫下番茄幼苗的已造成伤害,但尚未达到抑制盐胁迫下番茄幼苗生长的作用。【结论】 NaCl胁迫显著影响了番茄幼苗的正常生长,外源施加适宜浓度的GSNO能有效缓解盐胁迫对番茄幼苗的影响。以0.1 mmol/L GSNO施用效果最佳。

关键词: 番茄幼苗; 盐胁迫; S-亚硝基谷胱甘肽

Abstract: 【Objective】 To investigate the effects of different concentrations of S-nitrosoglutathione (GSNO) on the growth of tomato seedlings under salt stress in the hope of screening out the appropriate concentration to improve the salt tolerance of tomato. 【Methods】 The tomato variety Zhongshu 4 was used as the experimental material and nutrient solution cultivation method was used. Under the stress of NaCl (100 mmol/L), the leaves of tomato seedlings were sprayed with different concentrations of GSNO (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6 mmol/L). The effects of exogenous GSNO on the growth, root activity, chlorophyll content, malondialdehyde (MDA) content, electrolyte permeability and proline content of tomato seedlings under salt stress were determined. 【Results】 (1) NaCl stress significantly reduced plant height, stem diameter, dry weight, fresh weight, root activity, chlorophyll a, chlorophyll b and total chlorophyll content of tomato seedlings; significantly increased MDA content and proline content of tomato seedlings; had no significant effect on electrolyte permeability; (2) exogenous application of 0.1-0.3 mmol/L GSNO can effectively alleviate the inhibition of salt stress on Tomato Seedling Growth and improve salt adaptability by increasing chlorophyll content, root activity and proline content, reducing membrane lipid peroxidation and membrane integrity damage Although the application of GSNO did harm to the growth of Tomato Seedlings under salt stress, it did not inhibit the growth of Tomato Seedlings under salt stress. 【Conclusion】 NaCl stress significantly affected the normal growth of tomato seedlings, and exogenous GSNO with appropriate concentration could effectively alleviate the effect of salt stress on tomato seedlings. In this experiment, 0.1 mmol/L GSNO was the best.

Key words: tomato seedling; salt stress; S-nitrosoglutathione

中图分类号:

S641.3

孙艳芸, 朱普生, 刁明, 崔金霞, 徐巍, 刘慧英. 不同浓度GSNO对盐胁迫下番茄幼苗生长的影响[J]. 新疆农业科学, 2021, 58(4): 625-633.

SUN Yanyun, ZHU Pusheng, DIAO Ming, CUI Jinxia, XU Wei, LIU Huiying. Effects of Different Concentrations of S-nitrosoglutathione (GSNO) on the Growth of Tomato Seedlings under Salt Stress[J]. Xinjiang Agricultural Sciences, 2021, 58(4): 625-633.

参考文献 38

[1]	努尔曼·阿布拉,故丽米热·卡克什,沙比尔江,热萨拉提.西梅品种“法兰西”引种试验表现及栽培技术[J].新疆林业,2011,(6):24-25, 46.Nuerman Abula, Guolimire Kakesh, Shabierjiang Resalati. Ximei variety "France" introduction test performance and cultivation technology [J]. Xinjiang Forestry, 2011,(6): 24-25, 46.
[2]	刘建新,王鑫,李博萍.外源一氧化氮供体SNP对NaCl胁迫下黑麦草幼苗叶片抗坏血酸-谷胱甘肽循环的影响[J].草业学报,2010,19(2):82-88. LIU Jianxin, WANG Xin, LI Boping. Effects of exogenous nitric oxide SNP on Ascorbic acid-Glutathione cycle in ryegrass seedlings under NaCl stress [J].Acta Prataculturae Sinica, 2010, 19(2):82-88.
[3]	樊瑞苹,周琴,周波,等.盐胁迫对高羊茅生长及抗氧化系统的影响[J].草业学报,2012,21(1):112-117.FAN Ruiping, ZHOU Qin, ZHOU Bo, et al. Effects of NaCl stress on growth and antioxidant system of Festucaelata[J]. Acta Prataculturae Sinica, 2012, 21(1):112-117.
[4]	刘成刚.青川箭竹不同光保护途径与碳氮代谢对干旱胁迫的响应及磷素调控[D].北京:中国科学院大学,2014.LIU Chenggang. Responses of different photoprotection pathways and carbon and nitrogen metabolism to drought stress and regulation of phosphorus in Fargesia Qingchuan County [D]. Beijing: University of Chinese Academy of Sciences, 2014.
[5]	倪星.紫花苜蓿种质资源的耐盐性综合评价研究[D].宁夏:宁夏大学,2016.NI Xing. Comprehensive Evaluation of salt tolerance of Alfalfa Germplasm Resources [D]. Ningxia: Ningxia University, 2016.
[6]	Besson-Bard A, Pugin A, Wendehenne D. New insights into nitric oxide signaling in plants [J]. Annual Review of Plant Biology, 2008, (59): 21-39.
[7]	Bethke P C, Libourel I G L, Jones L. itric oxide reduces seed dormancy in Arabidopsis[J]. Journal of Experimental Botany, 2006, 57(3):517-526.
[8]	Delledonne M. NO news is good news for plants [J]. Current Opinion in Plant Biology, 2005,8(4):390-396.
[9]	Guo F Q,Crawford N M. Arabidopsis nitric oxide synthase1 is targeted to mitochondria and protects against oxidative damage and darkinduced senescence [J]. Plant Cell, 2005, 17(12):3436-3450.
[10]	Sokolovski S, Hills A, Gay R, et al, Protein phosphorylation is a prerequisite for intracellular Ca2+ release and ion channel control by nitric oxide and abscisic acid in guard cells [J]. Plant Journal, 2005, 43(4):520-529.
[11]	Huang X, Stettmaier K, Michel C, et al. Nitric oxide is induced by wounding and influences jasmonic acid signaling in Arabidopsis thaliana[J]. Planta, 2004, 218(6):938-946.
[12]	Grun S, Lindermayr C, Sell S, et al. Nitric oxide and gene regulation in plants [J]. Journal of Experimental Botany, 2006, 57(3):507-516.
[13]	Ruan H H, Shen W B, Ye M B, et al .Protectiv e effects of nitric oxide on salt stress-induced oxidative damage to wheat (Trriticum aestivumL .) leaves [J] .Chinese Science Bulletin, 2001, 46(23): 1993-1997.
[14]	樊怀福,郭世荣,焦彦生,等.外源一氧化氮对NaCl胁迫下黄瓜幼苗生长、活性氧代谢和光合特性的影响[J] .生态学报,2007, 27(2): 546-553. FAN Huaifu, GUO Shirong, JIAO Yansheng, et al. Effects of exogenous Nitric Oxide on growth, active oxygen metabolism and photosynthetic characteristics of cucumber seedlings under NaCl stress [J]. Acta Prataculturae Sinica, 2007,27(2):546-553.
[15]	吴雪霞,朱月林,朱为民, 等.外源一氧化氮对NaCl胁迫下番茄幼苗生理影响[J].中国农业科学, 2006, 39(3): 575-581.WU Xuexia, ZHU Yuelin, ZHU Weimin, et al. Effects of exogenous Nitric Oxide on physiology of tomato seedlings under NaCl stress [J]. Scientia Agricultura Sinica, 2006, 39(3):575-581.
[16]	孙立荣,郝福顺. 一氧化氮与植物耐盐性[J]. 植物生理学通讯,2009,45(10): 947-952.SUN Lirong, HAO Fushun. Nitric Oxide and plant Salt Tolerance [J]. Plant physiology, 2009, 45(10):947-952.
[17]	Cui Xiumin,Wu Xiaobing, Li Xiaoyun, et al. Responses of growth, functional enzyme activity in biomembrane of tomato seedlings to excessive copper, cadmium and the alleviating effect of exogenous nitric oxide [J]. Plant Nutrition and Fertilizer Science, 2011, 17(2):349-357.
[18]	Nagalakshmi N, Prasad MNV. Responses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus hijugatus[J]. Plant Sci, 2001, 160(2): 291-299.
[19]	Park, J. Reaction of S- nitrosoglutathione with sulfhydryl groups in protein [J]. Biochem. Biophys. Res. Comm., 1988, (152):916-920.
[20]	温泽林. 外源GSH介导NO调控番茄幼苗盐适应性研究[D].石河子:石河子大学,2018.WEN Zelin. Exogenous GSH mediates NO regulation on salt adaptation of Tomato Seedlings [D]. Shihezi: Shihezi University, 2018.
[21]	徐一馨,王飞,张成双,慕自新.谷胱甘肽存在条件下NO_2~-对玉米植株干旱胁迫的缓解作用[J].西北农林科技大学学报(自然科学版),2014,42(6):41-47, 54.XU Yixin, WANG Fei, ZHANG Chengshuang, et al. Effects of NO_2 ~ - on drought stress of maize plants under the presence of glutathione [J]. Journal of Northwest A & F University (Natural Science Ed.), 2014, 42(6) : 41-47, 54.
[22]	严洁萍. GSNO或诱生型NOS激活糖酵解通路及HIF-1α相关机制研究[D].杭州:浙江大学,2013.YAN Jieping. Study on glycolytic pathway activated by GSNO or inducible NOS and related mechanism of HIF-1 [D]. Hangzhou: Zhejiang University, 2013.
[23]	董志刚,程智慧.番茄品种资源芽苗期和幼苗期的耐盐性及耐盐指标评价[J].生态学报,2009,29(3):1348-1355.DONG Zhigang, CHENG Zhihui. Evaluation of salt tolerance and salt tolerance index of tomato germplasm resources at seedling stage and seedling stage [J]. Acta Ecologica Sinica, 2009, 29(3):1348-1355.
[24]	李合生,陈华癸.21世纪农林本科生物系列课程改革的研究与实践(上)[J].中国农业教育,2000,(4):19-22.LI Hesheng, CHEN Huakui. Research and practice on the reform of biology series courses for undergraduates majoring in agriculture and forestry in the 21st century [J]. Agricultural Education in China, 2000,(4):19-22.
[25]	张志良,瞿伟菁,李小方.植物生理学实验指导[M].北京:高等教育出版社,2009.ZHANG Zhiliang, QU Weijing, LI Xiaofang. Plant physiology. BeiJing: Higher Education Press, 2009.
[26]	Arnon D I.Copper enzymes in isolated chloroplast;polyph-enoloxidase in Beta vulgaris [J]. Plant Physiol, 1949, (24):1-15.
[27]	邹琦.植物生理学试验指导[M].北京:中国农业出版社,2000:159-130.ZOU Qi. Plant Physiology [M]. Beijing: China Agriculture Press, 2000:159-130.
[28]	余叔文,汤章成.植物生理与分子生物学[M].北京:科学出版社,1998.YU Shuwen, TANG Zhangcheng. Plant physiology and Molecular Biology [M]. Beijing: Science Press, 1998.
[29]	许卉,赵丽萍.盐胁迫对金银花生理生化的影响[J].湖北林业科技,2007,143(1):9-12.XU Hui, ZHAO Liping. Effects of salt stress on physiology and biochemistry of JinYinHua[J]. Hubei Forestry Science and Technology, 2007, 143(1):9-12.
[30]	郭金耀,杨晓玲.盐藻DNA对大豆幼苗耐盐性的影响[J].安徽农业科学,2011,39(25):15241-15242.GUO Jinyao,YANG Xiaoling. Effects of Dunaliella Salina DNA on salt tolerance of soybean seedlings [J]. Journal of Anhui Agricultural Sciences, 2011, 39(25):15241-15242.
[31]	王松,莘冰茹,周艳,等.外源NO对NaCl胁迫下番茄幼苗生长及光合特性的影响[J].石河子大学学报(自然科学版),2016,34(3):321-327.WANG Song, XIN Bingru, ZHOU Yan, et al. Effects of exogenous NO on growth and photosynthetic characteristics of tomato seedlings under NACL STRESS [J]. Journal of Shihezi University, 2016, 34(3):321-327.
[32]	Mir R R,Zaman-Allah M, Sreenivasulu N, et al. Integrated genomics,physiology and breeding approaches for improving drought tolerance in crops[J].Theoretical and Applied Genetics, 2012,(125):625-645.
[33]	张丽梅. 两种白头翁属植物种子发芽特性及幼苗抗旱性的研究[D].哈尔滨:东北林业大学,2013.ZHANG Limei. Study on seed Germination characteristics and seedling drought resistance of two species of Pulsatilla L.[D]. Harbin: Northeast Forestry University, 2013.
[34]	杨梅. 邻羟基苯甲酸胁迫对不同杉木无性系化感效应及差异蛋白质组分析[D].福州:福建农林大学,2007.YANG Mei. Allelopathic effects of salicylic acid stress on different Chinese fir clones and differential proteome analysis [D]. Fuzhou: Fujian Agriculture and Forestry University, 2007.
[35]	刘玉民. 酸铝环境马尾松根系分泌物特性及其缓解铝毒的根际效应[D].重庆:西南大学,2018.LIU Yumin. Characteristics of root exudates of Pinus Massoniana under al2o3 environment and rhizosphere effect of al2o3 Alleviation [D]. Chongqing: Southwest University, 2018.
[36]	段国锋,王争争,李丽娟,等.高压静电场对菜豆生理活性的影响[J].北方园艺,2014,(11):24-26.DUAN Guofeng,WANG Zhengzheng,LI Lijuan,et al. Effects of high voltage electrostatic field on physiological activity of Kidney Bean[J]. Northern Horticulture, 2014,(11):24-26.
[37]	Siddiqui M H, Al-Whaibi M H, Basalah M O. Role of nitric oxide in tolerance of plants to abiotic stress [J]. Protoplasma, 2011, (248):447-455.
[38]	王超,顾伟咏,安定,等.盐胁迫对番茄几个形态及生理指标的影响[J].安徽农业科学,2013,41(27):10911-10913, 10942.WANG Chao, GU Weiyong, AN Ding, et al. Effects of salt stress on several morphological and physiological indexes of Tomato [J]. Journal of Anhui Agricultural Sciences, 2013, 41(27):10911-10913, 10942.

不同浓度GSNO对盐胁迫下番茄幼苗生长的影响

Effects of Different Concentrations of S-nitrosoglutathione (GSNO) on the Growth of Tomato Seedlings under Salt Stress

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