红宝石无核×‘SP6164’杂交群体果实性状遗传倾向

doi:10.6048/j.issn.1001-4330.2019.09.005

摘要/Abstract

摘要： 【目的】研究红宝石无核葡萄果实性状的遗传规律。【方法】以红宝石无核×‘SP6164’杂交群体134个F₁代植株为试材,测定其种痕或种子大小、果实香味、果粒单重、果皮颜色、果粒纵横径、果实可溶性固形物含量、果粒的耐压力和果蒂的耐拉力等果实性状指标,分析群体核性、果实香味、果粒大小、果色、果形、果实含糖量、果实耐贮运性等指标的遗传倾向。【结果】群体55.97%的单株为无核,4.48%的为香型类,果粒重量呈趋中遗传趋势,91.8%的单株表现为增大,紫红-红紫的单株比例最高,为36.09%,主要趋向椭圆形,比例为55.9%,果实含糖量遗传力83.53%,有8.96%的超高亲单株,平均耐压力遗传力为83.05%,但有2.24%的超高亲单株,平均耐拉力遗传力为119.27%,具有27.61%的超高亲单株。【结论】红宝石无核与香味有核品系‘SP6164’杂交,可以获得高比例的无核后代和一定比例的香味后代,群体中各项性状指标均获得一定的加性效应表达,但在单粒重、耐拉力两项指标上表现的更显著。

关键词: 葡萄; 杂交群体; 果实性状; 遗传倾向

Abstract: 【Objective】 To explore the inheritance of the fruit traits of Ruby Seedless grape. 【Method】It evaluated the criteria of main fruit characteristics of the size of seed scar or seed, fruit fragrance, berry weight, surface color of pericarp, longitudinal and transverse diameter of fruit grains, content of soluble solids in fruits, stress tolerance of fruit grain and tensile tolerance of fruit pedicle by 134 progenies originating of Ruby Seedless × ''SP6164', and analyzed the genetic tendency of seed trait, fruit fragrance, berry weight, surface color of pericarp, fruit shape, sugar content in fruit, storage and transportation tolerance of fruits.【Result】Among the population, 55.97% were seedless, 4.48% were aromatic, and the weight of fruit grains tended to be moderately inherited. 91.8% were increased. The proportion of purple-red-purple was the highest (36.09%), mainly elliptic (55.9%), the heritability of fruit sugar content was 83.53%, and 8.96% of the super-high parents were inherited with average stress tolerance. The average heritability of pull tolerance was 119.27%, with 27.61% of the super-parent plants.【Conclusion】A large proportion of seedless progenies and a certain proportion of aroma progenies could be obtained by crossing Ruby Seedless with ''SP6164'', which was a fragrance line. The additive effect expression of each character index in the population could be obtained, but it was more significant in the two indexes of single grain weight and tensile endurance.

Key words: grape var. Ruby Seedless; hybrid population; fruit traits; inheritance trend

中图分类号:

S663.1

王勇, 李玉玲, 苏来曼·艾则孜, 孙锋, 伍国红, 骆强伟, 郭平峰. 红宝石无核×‘SP6164’杂交群体果实性状遗传倾向[J]. 新疆农业科学, 2019, 56(9): 1609-1618.

WANG Yong, LI Yu-ling, Sulaiman Aizezi, SUN Feng, WU Guo-hong, LUO Qiang-wei, GUO Ping-feng. Inheritance Trend of the Fruit Traits of Hybrid Populations of Ruby Seedless and 'SP6164'[J]. Xinjiang Agricultural Sciences, 2019, 56(9): 1609-1618.

参考文献

[1] 姜建福, 樊秀彩, 张颖, 等. 中国葡萄品种选育的成就与可持续发展建议[J].中外葡萄与葡萄酒, 2018,(1):60-67.
   JANG Jian-fu, FAN Xiu-cai, ZHANG Ying, et al. (2018). Achievements and sustainable development suggestions of grape breeding in China [J]. Sino-overseas Grapevine &Wine, (1):60-67. (in Chinese)
[2] 国家葡萄产业技术体系办公室. 国家葡萄产业技术体系启动大会在京召开[J]. 中外葡萄与葡萄酒, 2009,(3):75.
   NATIONAL Grape-industry-technical-system-office. (2009) The National Grape Industry Technology System Initiation Conference held in Beijing [J]. Sino-overseas Grapevine &Wine, (3):75. (in Chinese)
[3] 赵月玲, 程贯召, 王汉海. 无核葡萄的育种与胚挽救技术的运用[J]. 生物学通报, 2004, 39(9):15-16.
   ZHAO Yue-ling, CHENG Guan-zhao, WANG Han-hai. (2004). Breeding of seedless grape and application of Embryo Rescue Technology [J]. Bulletin of Biology, 39(9):15-16. (in Chinese)
[4] 王壮伟, 赵密珍, 吴伟民, 等. 无核葡萄胚珠培养技术研究[J]. 江西农业学报, 2007,(2): 27-29, 62.
   WANG Zhuang-wei, ZHAO Mi-zhen, WU Wei-min, et al. (2007). Study on techniques of ovule culture for seedless grape [J]. Acta Agriculturae Jiangxi, (2): 27-29, 62. (in Chinese)
[5] Ramming D. W., Emershad R. L.(1982). In-ovule embryo culture of seeded and seedless Vitis Vinifera L (Abst.). Hortscience, 17(3): 487.
[6] Ramming, D. W., Ledbetter, C. A. , & Tarailo, R. (1990). Hybridization of seedless grapes. Vitis, Special issue: 439-444.
[7] Garcia, E. , Martinez, A. , Garcia, d. l. C. E. , Perez, L. J. , Cenis, J. L. , & Carre?o, J, et al. (2000). In vitro culture of ovules and embryos of grape for the obtention of new seedless table grape cultivars. Acta Horticulturae, 25(528):663-666.
[8] P Ponce, M. T., Agüero, C.B., Gregori, M. T., & Tizio, R. (2000). Factors affecting the development of stenospermic grape (vitis vinifera) embryos cultured in vitro. Acta Horticulturae(528): 667-672.
[9] 郭印山, 高秀岩, 赵玉辉, 等. 金星无核葡萄胚挽救技术研究初报[J]. 中外葡萄与葡萄酒, 2004,(3):6-8.
   GUO Yin-shan, GAO Xiu-yan, ZHAO Yu-hui, et al.(2004). Preliminary report on the embryo rescue technique in venus seedless grape [J]. Sino-overseas Grapevine &Wine, (3):6-8.(in Chinese)
[10]王珊, 李荣, 樊新民, 等. 紫香无核胚培养技术研究[J]. 北方园艺, 2010,(2):148-150.
   WANG Shan, LI Rong, FAN Xin-min, et al. (2010). Study on Embryo Rescue of Zixiang Seedless Grape (Vitis vinifera L) [J]. Northern Horticulture, (2):148-150.(in Chinese)
[11]蒋爱丽, 李世诚, 金佩芳, 等. 无核葡萄"喜乐"胚挽救培养研究与应用[J]. 上海农业学报, 2010, 26(4):39-42.
   JIANG Ai-li, LI Shi-cheng, JIN Pei-fang, et al. (2010). Research and application of the embryo reseue of seedless grape "Himrord Seedless"[J]. Acta Agriculturae Shanghai, 26(4):39-42. (in Chinese)
[12]武书哲, 张娜, 田淑芬. 无核葡萄希姆劳特胚挽救中影响胚发育的因子[J]. 河北林业科技,2014,(5&6):41-43.
   WU Shu-zhe, ZHANG Na, TIAN Shu-fen. (2014). Factors affecting embryo development in embryo rescue of seedless grape [J]. The Journal of Hebei Forestry Science & Technology, (5&6):41-43.(in Chinese)
[13]潘学军, 李顺雨, 张文娥, 等. '森田尼无核'葡萄胚发育及败育的细胞学特征[J]. 北方园艺, 2011,(5):41-43.
   PAN Xue-jun, LI Shun-yu, ZHANG Wen-e, et al. (2011). Cytological study on embryo development and abortion in 'stenospermocarpic' grapes [J]. Northern Horticulture, (5):41-43.(in Chinese)
[14]李玉玲, 王勇, 孙锋, 等. 火洲黑玉和品系SP87-13-3胚挽救体系的建立[J]. 中外葡萄与葡萄酒, 2013, (3):18-21.
   LI Yu-ling, WANG Yong, SUN Feng, et al. (2013). Study on the embryo rescue technology system of 'Huozhouheiyu' and strain 87-13-3[J]. Sino-overseas Grapevine & Wine, (3):18-21.(in Chinese)
[15]王庆莲, 吴伟民, 赵密珍, 等. 无核葡萄及其杂交组合胚挽救技术[J]. 江苏农业学报, 2015, 31(6):1 413-1 418.
   WANG Qing-lian, WU Wei-min, ZHAO Mi-zhen, et al. (2015). Embryo rescue techniques for seedless grapes and reciprocal cross combinations [J]. Jiangsu J.of Agr. Sci. , 31(6):1,413-1,418.(in Chinese)
[16]田淑芬, 温晓敏, 张娜, 等. 无核葡萄自交胚挽救技术研究[J]. 中外葡萄与葡萄酒, 2017,(4):25-27.
   TIAN Shu-fen, WEN Xiao-min, ZHANG Na, et al. (2017). The studies on embryo rescue techniques of seedless grape selfing [J]. Sino-overseas Grapevine &Wine, (4):25-27. (in Chinese)
[17]史文静, 骆强伟, 王跃进. 无核香味葡萄胚挽救育种研究[J]. 西北植物学报, 2018, 38(6):983-993.
   SHI Wen-jing, LUO Qiang-wei, WANG Yue-jin. (2018). Breeding grapevine varieties for seedlessness with flavour using embryo rescue [J]. Acta Botanica Boreali-Occidentalia Sinica , 38(6):983-993. (in Chinese)
[18]孔庆山. 中国葡萄志[M]. 北京: 中国农业科学技术出版社, 2004: 340.
   KONG Qing-shan. (2004). Chinese grape flora [M]. Beijing: China Agricultural Science and Technology Press: 340.(in Chinese)
[19]张剑侠, 牛茹萱. 无核葡萄胚挽救技术的研究现状与展望[J]. 园艺学报, 2013, 40(9):1 645-1 655.
   ZHANG Jian-xia, NIU Ru-xuan. (2013). The present situation and prospect of embryo rescue technique research in seedless grape breeding [J]. Acta Horticulturae Sinica, 40(9):1,645-1,655.(in Chinese)
[20]蒋爱丽, 李世诚, 金佩芳, 等. 胚培无核葡萄新品种--沪培1号的选育[J]. 果树学报, 2007, 24(3):402-403.
   JIANG Ai-li, LI Shi-cheng, JIN Pei-fang, et al. (2007). Hupei l--a new triploid seedless grape cultivar obtained by embryo culture [J]. Journal of Fruit Science, 24(3):402-403. (in Chinese)
[21]蒋爱丽, 李世诚, 杨天仪, 等. 无核葡萄新品种一沪培2号的选育[J]. 果树学报, 2008, 25(4):618-619.
   Jiang Ai-li, Li Shi-cheng, Yang Tian-yi, et al. (2008). A new seedless grape cultiva--Hupei No.2 [J]. Journal of Fruit Science,25(4): 618-619. (in Chinese)
[22]蒋爱丽, 奚晓军, 程杰山, 等.早熟葡萄新品种--'申爱'的选育[J]. 果树学报, 2014, 31(2):335-336.
   JIANG Ai-li, XI Xiao-jun, CHENG Jie-shan, et al. (2014). Breeding of a new early-ripening grape variety - 'Shen Ai' [J]. Journal of Fruit Science,31(2):335-336. (in Chinese)
[23] 月丫, 张娜, 田淑芬, 等. '奥迪亚无核'与玫瑰香香型葡萄杂交胚挽救技术研究[J]. 中外葡萄与葡萄酒, 2017,(6):22-25.
   YUE Ya, ZHANG Na, TIAN Shu-fen, et al.(2017). Study on embryo rescue techniques of hybrid grape of 'Audia Seedless' and rose fragrance [J]. Sino-overseas Grapevine &Wine, (6):22-25. (in Chinese)
[24] 田淑芬, 月丫, 张娜, 等. '红宝石无核'与玫瑰香香型葡萄杂交胚挽救技术研究[J]. 中外葡萄与葡萄酒, 2018,(4):61-64.
   TIAN Shu-fen , YUE Ya, ZHANG Na, et al. (2018). Study on embryo rescue techniques of 'Ruby Seedless'and rose fragrant grape hybrids [J]. Sino-overseas Grapevine & Wine, (4):61-64. (in Chinese)
[25] 李顺雨, 潘学军, 张文娥, 等. '红宝石无核'胚珠败育的直观形态学研究[J]. 北方园艺, 2009,(4):33-36.
   LI Shun-yu, PAN Xue-jun, ZHANG Wen-e, et al. (2009). A visual morphological study on ovule abortion of `Ruby Seedless'[J]. Northern Horticulture, (4):33-36. (in Chinese)
[26] 赵密珍, 苏家乐, 钱亚明, 等. '红宝石无核'葡萄胚珠培养成苗技术研究[J]. 果树学报, 2005, 22(2):166-168.
   ZHAO Mi-zhen, SU Jia-le, QIAN Ya-ming, et al. (2005). Study on ovule culture and seedling formation of 'Ruby Seedless' grape [J]. Journal of Fruit Science, 22(2):166-168. (in Chinese)
[27] 温晓敏, 张娜, 路凤珍, 等. '红宝石无核'和'希姆劳特'葡萄杂交胚挽救技术研究[J]. 天津师范大学学报(自然科学版), 2017, 37(5):32-35.
   WEN Xiao-min, ZHANG Na, LU Feng-zhen, et al. (2017). Study on embryo rescue techniques of 'Ruby Seedless'and 'Himlaut' Grape Hybrids [J]. Journal of Tianjin Normal University (Natural Science Edition), 37(5):32-35. (in Chinese)
[28] 潘学军, 李顺雨, 张文娥, 等. '红宝石无核'葡萄胚发育及败育的观察[J]. 种子, 2011, 30(11):66-67.
   PAN Xue-jun, LI Shun-yu, ZHANG Wen-e, et al. (2011). Observation on embryo development and abortion of 'Ruby Seedless' grape [J]. Seed, 30(11):66-67. (in Chinese)
[29] 刘崇怀, 沈育杰, 陈俊. 葡萄种质资源描述规范和数据标准[M]. 北京:中国农业出版社, 2006: 98-102.
   LIU Chong-huai, SHEN Yu-jie, CHEN Jun. (2006). Descriptors and data standard for grape(Vitis L.) [M]. Beijing: China Agriculture Press:98-102. (in Chinese)
[30] 贺普超. 葡萄学[M]. 北京:中国农业出版社, 1999: 265,269-270,273-275.
   HE Pu-chao. (1999). Viticulture [M]. Beijing: China Agricultural Press, 265,269-270,273-275. (in Chinese)
[31]王勇, 苏来曼·艾则孜, 李玉玲, 等. '火州黑玉'葡萄杂交后代果实性状遗传倾向分析[J]. 西北植物学报, 2015, 35(2): 275-281.
   WANG Yong, SULAIMAN Ai-ze-zi, LI Yu-ling, et al. (2015). Inheritance trend of the fruit traits of 'Huozhouheiyu' Grape[J]. Acta Bot. Boreal.-Occident. Sin., 35(2): 275-281. (in Chinese)
[32]谭伟, 李晓梅, 唐晓萍, 等. 鲜食葡萄杂交后代果实玫瑰香香味表型的遗传倾向初报[J]. 中外葡萄与葡萄酒, 2013,(4):6-9.
   TAN Wei, LI Xiao-mei, TANG Xiao-ping, et al. (2013). Preliminary study on the inheritance of the muscat flavor phenotype in hybrids of table grapes [J]. Sino-Overseas Grapevine & Wine, 4:6-9. (in Chinese)
[33] 郭修武, 郭印山, 李轶晖, 等. 葡萄杂交后代主要经济性状的遗传倾向[J]. 果树学报, 2004, 21(4):319-323.
   GUO Xiu-wu, GUO Yin-shan, LI Yi-hui, et al. (2004). Studies on the inheritance trend of the main economic characteristics of crossed grape progeny [J]. Journal of Fruit Science, 21(4):319-323. (in Chinese)
[34] Barritt, B HEinset, JBarritt, B. H. and Einset, J. (1969). The inheritance of three major fruit color in grapes. Journal of the American Society for Horticultural Science (1969) 94(2) :87-89.
[35] Dauelsberg, P. , José Tomás Matus, María Josefina Poupin, Andrés Leiva-Ampuero, Godoy, F. , & Vega, A. , et al. (2011). Effect of pollination and fertilization on the expression of genes related to floral transition, hormone synthesis and berry development in grapevine. Journal of Plant Physiology,168(14):1,667-1,674.
[36] Rodriguez, G. R. , Munos, S. , Anderson, C. , Sim, S. C. , Michel, A. , & Causse, M. , et al. (2011). Distribution of sun, ovate, lc, and fas in the tomato germplasm and the relationship to fruit shape diversity. Plant Physiology, 156(1):275-285.
[37] 张恒, 刘众杰, 樊秀彩, 等. 葡萄果粒形状简化基因组关联分析[J]. 园艺学报, 2017, 44 (10):1 959-1 968.
   ZHANG Heng, LIU Zhocg-jie, FAN Xiu-cai, et al. (2017). Genome-wide association mapping of berry shape traits via the reduced representation sequencing in grape [J]. Acta Horticulturae Sinica, 44 (10):1,959-1,968. (in Chinese)
[38] 张培安, 樊秀彩, 刘众杰, 等. 葡萄种质资源果形性状的分析[J]. 园艺学报, 2018, 45 (8):1 456-1 466.
   ZHANG Pei-an, FAN Xiu-cai, LIU Zhong-jie, et al. (2018). Investigation and analysis on the berry shape of grape germplasm resources [J]. Acta Horticulturae Sinica, 45(8):1,56-1,466. (in Chinese)
[39] Cléa Houel, M.. Martin agniette, Stéphane D. Nicolas, Lacombe, T. , & A. Adam London. (2013). Genetic variability of berry size in the grapevine (vitis vinifera l.). Australian Journal of Grape and Wine Research, 19(2):208-220.
[40] 罗素兰, 贺普超. 葡萄种间杂交一代果穗果粒性状的遗传[J]. 中外葡萄与葡萄酒, 1999, (4):17-21.
    LUO Su-lan, HE Pu-chao. (1999). Inheritance of the traits of ear and grains in the first generation of interspecific hybridization of grape [J]. Sino-Overseas Grapevine & Wine, 4:17-21. (in Chinese)
[41] 王军, 宋润刚, 尹立荣, 等. 山葡萄与欧亚种葡萄杂交后代性状遗传[J]. 特产研究, 1997, (1):5-9.
   WANG Jun, SONG Run-gang, YI Li-rong, et al. (1997). Inheritance of characters in hybrid progenies of vitis amurensis and Eurasian grape [J]. Special Wild Economic Animal and Plant Research, (1):5-9. (in Chinese)