新疆牛羊源金黄色葡萄球菌D353质粒pD353序列分析

doi:10.6048/j.issn.1001-4330.2023.07.029

摘要/Abstract

摘要：

【目的】分析金黄色葡萄球菌耐甲氧西林菌株和耐万古霉素菌株的野生型质粒序列特征,为研究超级耐药菌提供参考。【方法】多重耐药菌株D353通过16S rRNA鉴定菌种,应用BLAST、MEGA 11、Mauve和trf等软件,分析多重耐药菌株D353的质粒pD353进行序列特征、基因、功能及复制方式。【结果】pD353质粒大小为34 063 bp,其G+C mol%含量为30.42%。pD353质粒与金黄色葡萄球菌的质粒序列pIT1-S和pIT4-R有高度同源性,其存在丰富的重金属转运蛋白和插入序列转座酶,其rep蛋白与pSK639家族质粒有高度同源性。【结论】该质粒为Theta复制,且未发现抗生素抗性基因。

关键词: 金黄色葡萄球菌; 多重耐药菌株; 质粒; 重复序列

Abstract:

【Objective】 To analyze the wild plasmid sequence characteristics of Staphylococcus aureus methicillin-resistant strains and vancomycin-resistant strains in the hope of helping the study of the formation of super-drug-resistant bacteria.【Methods】 The multidrug-resistant strain D353 was identified by 16s rRNA, and the sequence features, genes, function and replication of the plasmid pD353 were analyzed by BLAST, MEGA 11, Mauve, and trf.【Results】 The results showed that the plasmid size from pD353 was 34,063 bp, the content of G-C mol was 30.42%.Plasmid sequence analysis found that pD353 plasmid and Staphylococcus aureus plasmid sequence pIT1-S and pIT4-R had a high homologous, which was rich in heavy metal transport protein and insertion sequence translocation enzyme, its rep protein and pSK639 family plasmids had a high homologous.【Conclusion】 Series repeat sequence analysis initially concluds that the plasmid is a θ replication and antibiotic resistance gene is unfound.

Key words: Staphylococcus aureus; multidrug-resistant strains; plasmids; repeat sequence

中图分类号:

S855.1

贺腾飞, 刘英玉, 张柳青, 陈旺, 李泽亚, 胡芸, 蒋金豆, 祖力胡马尔·艾力. 新疆牛羊源金黄色葡萄球菌D353质粒pD353序列分析[J]. 新疆农业科学, 2023, 60(7): 1806-1812.

HE Tengfei, LIU Yingyu, ZHANG Liuqing, CHEN Wang, LI Zeya, HU Yun, JIANG Jindou, Zulihumaer Aili. Analysis of sequence analysis of pD353 plasmids of Staphylococcus aureus D353, the source of cattle and sheep in Xinjiang[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1806-1812.

图/表 5

图1 质粒pD353 16S rRNA系统发育树

Fig.1 plasmid pD353 16S rRNA phylogenetic tree

图2 pD353质粒圈

Fig.2 pD353 plasmid circle diagram

图3 质粒序列共线性

Fig.3 Plasmid sequence collinearity analysis graph

图4 质粒pD353 repB 系统发育树

Fig.4 plasmid pD353 repB Phylogenetic tree

图5 质粒pD353复制起点

Fig.5 Starting point map of plasmid pD353 replication

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