阿克苏野生牛肝菌多糖提取优化及不同组分抗氧化活性分析

doi:10.6048/j.issn.1001-4330.2023.07.030

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (7): 1813-1820.DOI: 10.6048/j.issn.1001-4330.2023.07.030

• 微生物·畜牧兽医 • 上一篇

阿克苏野生牛肝菌多糖提取优化及不同组分抗氧化活性分析

冯倩¹(), 何齐¹, 李雪², 易鸳鸯², 孙建², 朱静², 顾美英², 谭慧林³, 张志东¹^,²()

1.新疆农业大学食品科学与药学学院,乌鲁木齐 830052
2.新疆农业科学院微生物应用研究所/新疆特殊环境微生物重点实验室,乌鲁木齐 830091
3.阿克苏地区食品安全检测中心,新疆阿克苏 843000

收稿日期:2022-10-11 出版日期:2023-07-20 发布日期:2023-07-11
通信作者: 张志东
作者简介:冯倩(1995-),女,新疆人,硕士研究生,研究方向为食品科学与食品安全, (E-mail)534675240@qq.com
基金资助:
国家重点研发计划项目(2018YFC1603400);新疆农业科学院科技创新重点培育专项(xjkcpy-2021002)

Extraction and antioxidant activity of different polysaccharide components from the wild boletus in Aksu region

FENG Qian¹(), HE Qi¹, LI Xue², YI Yuanyang², SUN Jian², ZHU Jing², GU Meiying², TAN Huilin³, ZHANG Zhidong¹^,²()

1. College of Food and Pharmaceutical Sciences, Xinjiang Agricultural University, Urumqi 830052, China
2. Xinjiang Key Laboratory of Special Environmental Microbiology/Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
3. Food Safety Testing Center of Aksu Region, Aksu Xinjiang 843000, China

Received:2022-10-11 Online:2023-07-20 Published:2023-07-11
Correspondence author: ZHANG Zhidong
Supported by:
Key R&D Program project of China(2018YFC1603400);Key Cultivation Project for Scientific and Technological Innovation of Xinjiang Academy of Agricultural Science(xjkcpy-2021002)

摘要/Abstract

摘要：

【目的】研究阿克苏野生牛肝菌多糖的最佳提取工艺,分析不同多糖组分的理化性质和抗氧化能力,为野生牛肝菌多糖的深入开发及利用提供科学参考。【方法】以阿克苏地区野生牛肝菌为材料,优化多糖水提条件,采用体积分数为40% 和 80% 的乙醇进行分级沉淀,得到2种粗多糖组分 LD-40 和 LD-80;测定多糖的水溶性、持水力和粘度,清除DPPH自由基、羟基自由基进行还原力试验,评价2种多糖组分的特性。【结果】牛肝菌多糖的最佳提取工艺条件为料液比1:30,温度80℃,时间3 h,多糖最高得率为13.36%。乙醇分级沉淀获得LD-40和 LD-80分别占总多糖的92.37%和7.63%,各自水溶性分别为66.9%和74.9%,持水力为7.71和6.16 g/g。相同浓度下,LD-40粘度较LD-80更强,但LD-80抗氧化能力比LD-40强。【结论】阿克苏地区野生牛肝菌富含多糖,具有较好的持水力,但抗氧化活性较弱。

关键词: 牛肝菌; 多糖; 分级醇沉

Abstract:

【Objective】 To optimize the extraction conditions of polysaccharides from the wild boletus in Aksu region and determine the physical properties and antioxidant capacity of different polysaccharide components. 【Methods】 The wild boletus was sampled from Aksu region,and water extraction conditions of polysaccharide were optimized. Two polysaccharide components,LD-40 and LD-80,were obtain by fractional precipitation of ethanol with volume fraction of 40% and 80%. The polysaccharide properties of water solubility,water retention and viscosity were tested. The antioxidant capacity was evaluated by free radical scavenging of DPPH and hydroxyl and reducing power experiments. 【Results】 The optimal extraction conditions were solid-liquid ratio 1∶30,temperature at 80℃,time for 3 h,then the yield of polysaccharide was 13.36%. Polysaccharide components,LD-40 and LD-80,obtained from ethanol fractional precipitation accounted for 92.37% and 7.63% of the total polysaccharide,respectively. The water solubility of LD-40 and LD-80 was 66.9% and 74.9%,respectively,and the water holding capacities were 7.71 and 6.16 g/g,respectively. At the same concentration,the viscosity of LD-40 was stronger than that of LD-80,but LD-80 had stronger antioxidant capacity than LD-40.【Conclusion】 The wild boletus in Aksu region is rich in polysaccharides which has good water holding capacity,but the antioxidant activity is weak. The results can provide scientific reference for the further development and utilization of wild boletus polysaccharides.

Key words: boletus; polysaccharide; fractional precipitation

中图分类号:

S567.3

冯倩, 何齐, 李雪, 易鸳鸯, 孙建, 朱静, 顾美英, 谭慧林, 张志东. 阿克苏野生牛肝菌多糖提取优化及不同组分抗氧化活性分析[J]. 新疆农业科学, 2023, 60(7): 1813-1820.

FENG Qian, HE Qi, LI Xue, YI Yuanyang, SUN Jian, ZHU Jing, GU Meiying, TAN Huilin, ZHANG Zhidong. Extraction and antioxidant activity of different polysaccharide components from the wild boletus in Aksu region[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1813-1820.

图/表 12

图1 阿克苏杨树林下野生牛肝菌

Fig.1 Wild boletus under the poplar forest in Aksu Region

表1 牛肝菌粗多糖提取条件优化正交试验因素与水平

Tab.1 Factors and levels of orthogonal tests for extraction optimization of polysaccharide from the wild Boletus

水平 Level	因素Factor
水平 Level	A料液比 (g:mL)	B提取温度 (℃)	C提取时间 (h)
1	1∶20	75	2
2	1∶25	80	3
3	1∶30	85	4

图2 多糖测定工作曲线

Fig.2 Standard curve for polysaccharide determination

图3 不同提取温度下多糖得率变化

Fig.3 Effect of extraction temperature on the yield of polysaccharides

图4 不同料液比下多糖得率变化

Fig.4 Effect of solid-liquid ratio on the yield of polysaccharides

图5 不同提取时间下多糖得率变化

Fig.5 Effect of extraction time on the yield of polysaccharides

表2 牛肝菌粗多糖提取正交试验

Tab.2 Results and analysis of orthogonal tests for extraction conditions optimization of polysaccharides from Boletus

试验 Test	A	B	C	多糖得率 Polysaccharide yield(%)
1	1	1	1	8.58
2	1	2	2	12.77
3	1	3	3	11.25
4	2	1	2	12.92
5	2	2	3	11.16
6	2	3	1	10.70
7	3	1	3	11.33
8	3	2	1	11.75
9	3	3	2	11.86
k₁	10.867	10.943	10.343
k₂	11.593	11.893	12.517
k₃	11.647	11.27	11.247
极差R Range	0.78	0.95	2.174

表3 不同多糖组分的水合性质

Tab.3 Hydration properties of different polysaccharide components

特性 Character	多糖组分 Polysaccharide components
特性 Character	LD-40	LD-80
水溶性 Water solubility(%)	66.6±3.45	74.9±2.35
持水力 Water holding capacity(g/g)	7.71±0.37	6.16±0.43

图6 不同浓度下各多糖组分粘度变化

Fig.6 Viscosity of each polysaccharide component at different concentrations

图7 不同浓度下各多糖组分对DPPH自由基清除变化

Fig.7 Effects of polysaccharide components at different concentrations on DPPH radicals scavenging activity

图8 不同浓度下各多糖组分对羟基自由基清除变化

Fig.8 Effects of polysaccharide components at different concentrations on OH radicals scavenging activity

图9 不同浓度各多糖组分的还原能力

Fig.9 Reducing power of the polysaccharide components at different concentrations

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阿克苏野生牛肝菌多糖提取优化及不同组分抗氧化活性分析

Extraction and antioxidant activity of different polysaccharide components from the wild boletus in Aksu region

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