不同产地黄芪活性成分与硒含量比较分析

doi:10.6048/j.issn.1001-4330.2021.05.011

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (5): 873-881.DOI: 10.6048/j.issn.1001-4330.2021.05.011

• 园艺特产·土壤肥料·植物保护 • 上一篇下一篇

不同产地黄芪活性成分与硒含量比较分析

靳元元^1,2, 马生军¹, 徐万里², 张军³, 耿阳¹, 吴文平¹, 王雪双¹

1.新疆农业大学食品科学与药学学院,乌鲁木齐 830052;
2.新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐 830092;
3.特克斯砺剑锋农林科技有限公司 ,新疆特克斯 835500

收稿日期:2020-03-11 出版日期:2021-05-20 发布日期:2021-05-26
通信作者: 马生军(1977-),男,新疆乌鲁木齐人,副教授,博士,研究方向为中药材规范化生产及质量调控机制,(E-mail)wlmqmsj@sina.com徐万里(1971-),男,陕西宝鸡人,研究员,博士,研究方向为土壤培肥与改良,(E-mail)wlxu2005@163.com
作者简介:靳元元(1994-),女,河北人,硕士研究生,研究方向为中药材规范化生产及质量调控机制,(E-mail)1435380155@qq.com
基金资助:
新疆维吾尔自治区科技成果转化示范专项-科技特派员扶贫行动“富硒农产品标准化生产技术推广应用”(2019C01020); 2019年度中国农业大学-新疆农业大学科研合作基金项目“根瘤菌接种甘草和黄芪的增效减肥机制及绿色化种植技术示范”; 2020年度校企合作技术基金项目“富硒黄芪生产关键技术及产品开发研究”

Comparative Analysis of Active Ingredients and Selenium Contents in Astragalus from Different Habitats

JIN Yuanyuan^1,2, MA Shengjun¹, XU Wanli², ZHANG Jun³, GENG Yang¹, WU Wenping¹, WANG Xueshuang¹

1. College of Food Sciences and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China;
2. Institute of Soil Fertilizer and Agricultural Water Conservation,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;
3. Tex Lijianfeng Agroforestry Technology Co. , Ltd,Tekes Xinjiang 835500,China

Received:2020-03-11 Online:2021-05-20 Published:2021-05-26
Correspondence author: MA Shengjun (1977-), male, from Urumqi, Xinjiang, associate professor, Doctor of Medicine, mainly engaged in the research of standardized production and quality control mechanism of Chinese medicinal materials, (E-mail) wlmqmsj @ Sina. com XU Wanli (1971-), male, Baoji, Shaanxi,researcher, Doctor of Agriculture, mainly engaged in soil fertility and improvement research, (E-mail) wlxu2005@ 163.com
Supported by:
Special Demonstration Project for Transformation of Scientific and Technological Achievements in Xinjiang Uygur Autonomous Region "Popularization and Application of Standardized Production Technology of Selenium Rich Agricultural Products" (2019C01020); 2019 China Agricultural University -Xinjiang Agricultural University Scientific Research Cooperation Fund Project "Rhizobium Inoculation Licorice and Astragalus in synergistic Weight Loss Mechanism and Green Planting Technology Demonstration"; 2020 School-Enterprise Cooperation Technology Fund Project "Selenium-Enriched Astragalus Production Key Technology and Product Development Research"

摘要/Abstract

摘要： 【目的】研究不同产地黄芪药材中活性成分与硒元素的含量,分析不同产地黄芪之间的差异及黄芪中硒含量与活性成分之间的相关性,为富硒黄芪的种植及资源开发利用提供参考依据。【方法】采用紫外分光光度法测定黄芪总皂苷、总黄酮、总多糖含量,高效液相色谱法(HPLC)测定不同产地黄芪药材中毛蕊异黄酮葡萄糖苷含量,氢化物发生-原子荧光法测定硒的含量,并对各指标进行统计分析。【结果】山西平鲁区黄芪总皂苷含量最高为1.557%,新疆昭苏县黄芪总黄酮含量最高为0.472%,宁夏隆德县黄芪毛蕊异黄酮葡萄糖苷含量最高为0.091%,新疆博湖县总多糖含量和硒含量均是最高,分别为27.617%和0.323 mg/kg;硒含量与总多糖含量呈极显著正相关(P<0.01),聚类分析中黄芪样品可按照不同成分的含量差异分为3类。【结论】22个不同产地黄芪活性成分含量与硒元素的含量有显著差异(P<0.05)。

关键词: 蒙古黄芪; 总皂苷; 总黄酮; 多糖; 硒

Abstract: 【Objective】 To explore the content of active ingredients and selenium in Astragalus membranaceus from different places and clarify the difference between Astragalus in different places and the correlation between selenium content in Astragalus and active ingredients. 【Methods】 UV spectrophotometry was used to determine the content of total saponins, total flavonoids and total polysaccharides in Astragalus membranaceus, high performance liquid chromatography (HPLC) was applied to determine the content of Calycosin-7-Glucosidein Astragalus membranaceus from different places, and hydride generation-atomic fluorescence method was employed to determine selenium content. After that, statistical analysis of each index was performed. 【Results】 The highest total saponin content of Astragalus in Pinglu district of Shanxi was 1.557%, the highest total flavonoid content of Astragalus in Zhaosu County, Xinjiang was 0.472%, the highest content of Calycosin-7-Glucosidein Astragalus Verticillus in Longde County, Ningxia was 0.091%. Both total polysaccharide content and selenium content were the highest in Bohu County, Xinjiang, which were 27.617% and 0.323 mg/kg, respectively. Correlation analysis showed that the content of selenium and total polysaccharide content were extremely significantly positively correlated (P<0.01). The content differences could be divided into 3 categories. 【Conclusion】 There are significant differences between the active ingredient content of Astragalus and selenium content in 22 different places (P<0.05). The relationship between selenium in Astragalus and total polysaccharide and total saponin content is worthy of more in-depth study, which might provide reference for planting and resource development and utilization.

Key words: Mongolian astragalus; total saponins; total flavonoids; polysaccharides; selenium

中图分类号:

靳元元, 马生军, 徐万里, 张军, 耿阳, 吴文平, 王雪双. 不同产地黄芪活性成分与硒含量比较分析[J]. 新疆农业科学, 2021, 58(5): 873-881.

JIN Yuanyuan, MA Shengjun, XU Wanli, ZHANG Jun, GENG Yang, WU Wenping, WANG Xueshuang. Comparative Analysis of Active Ingredients and Selenium Contents in Astragalus from Different Habitats[J]. Xinjiang Agricultural Sciences, 2021, 58(5): 873-881.

参考文献 27

[1]	张蔷,高文远,满淑丽. 黄芪中有效成分药理活性的研究进展[J]. 中国中药杂志,2012,37(21) : 3203-3206. ZHANG Qiang,GAO Wenyuan,MAN Shuli. Chemical composition and pharmacological activities of Astragali Radix [J]. China Journal of Chinese Materia Medica,2012,37(21): 3203-3206.
[2]	王辰,侯连兵. 黄芪新剂型的临床应用[J]. 医药导报,2006,25(6):526. WANG Chen,HOU Lianbing. Clinical application of Astragalus new formulation [J]. Herald of Medicine,2006,25(6): 526.
[3]	国家药典委员会. 中华人民共和国药典:一部[M]. 2015版. 北京:化学工业出版社,2015: 302. National Pharmacopoeia Commission. Pharmacopoeia of the people's republic of China: One part(2015 Ed.) [M]. Beijing: Chemical Industry Press,2015:302.
[4]	刘力进,唐萌萌,谢宏晨,等. 富硒无花果提取物片急性毒性及体外肿瘤细胞生长抑制试验研究[J]. 食品安全质量检测学报,2019,10(21):7234-7239. LIU Lijin,TANG Mengmeng,XIE Hongchen,et al. Experimental study on actue toxicity and inhibition of tumor cell growth in virto of selenium-enriched fig extract tablet [J]. Journal of Food Safety and Quality,2019,10(21): 7234-7239.
[5]	杨翠玲,郭爱华. 不同等级浑源黄芪中黄芪甲苷及硒元素含量测定[J]. 中国中药杂志,2011,36(13):1 720-1 721. YANG Cuiling,GUO Aihua. Determination of Astragaloside IV and selenium in Astragalus membranaceus in different grades [J]. China Journal of Chinese Materia Medica,2011,36(13): 1720-1721.
[6]	吴应亮,蔡宗源,何康明,等. 富硒植物中硒化合物的提取和分析[J]. 暨南大学学报(自然科学与医学版),1994,(3):70-73. WU Yingliang,CAI Zongyuan,HE Kangming,et al. Stuadies on selenium compounds in some selenium-rich plants [J]. Journal of Jinan University (Natural Science and Medicine Ed.),1994,(3): 70-73.
[7]	邵杰敏,连玉菲,阎乐浩,等.不同产地黄芪中的黄芪总皂苷含量对比分析[J].陕西中医,2018,39(2):268-270.SHAO Jiemin,LIAN Yufei,YAN Lehao,et al. Comparative analysis of total saponins in Astragalus membranaceus from different habitats [J]. Shaanxi Journal of Traditional Chinese Medicine,2018,39(2): 268-270.
[8]	程研,闵会,何厚洪,等.紫外分光光度法测定不同产地黄芪总黄酮的含量[J].江西中医药大学学报,2015,27(1):58-60.CHENG Yan,MIN Hui,HE Houhong,et al. Determination of flavonoid in Astragalus grown in different areas by UV Spectrophotometry [J]. Journal of Jiangxi University of Traditional Chinese Medicine,2015,27(1): 58-60.
[9]	丁慧,许纪锋,沈君子,等.黄芪中毛蕊异黄酮葡萄糖苷的含量测定[J].食品安全质量检测学报,2017,8(1):223-226.DING Hui,XU Jifeng,SHEN Junzi,et al. Determination of calycosin-7-glucoside in Astragalus membranaceus [J]. Journal of Food Safety and Quality,2017,8(1): 223-226.
[10]	张艳贺,张雷鸣,刘秀波,等.不同产地黄芪中黄芪多糖的含量测定[J].中医药信息,2013,30(5):69-71.ZHANG Yanhe,ZHANG Leiming,LIU Xiubo,et al. Content determination of Astragalus polysaccharide from Astragalus in different regions [J]. Information on Chinese Medicine,2013,30(5) : 69-71.
[11]	耿丽平,乔斌,赵全利,等. 土培条件下外源硒对黄芪吸收和转运硒的影响[J]. 水土保持学报,2018,32(2):372-376. GENG Liping,QIAO Bin,ZHAO Quanli,et al. Effect of exogenous Se on the uptake and translocation of Se in Astragalusmembranaceus using soil pot expriment [J]. Journal of Soil and Water Conservation,2018,32(2): 372-376.
[12]	罗定祥,冶军,侯振安,等. 不同有机物料对土壤硒形态及小麦硒吸收的影响[J]. 新疆农业科学,2018,55(2):328-336.LUO Dingxiang,YE Jun,HOU Zhenan,et al. Effects of different organic materials on the forms selenium and the absorption of selenium in wheat [J]. Xinjiang Agricultural Sciences,2018,55(2): 328-336.
[13]	樊英,于晓清,王淑娴,等. 不同剂型的黄芪多糖在刺参养殖中的应用研究[J]. 水产科学,2012,31(11):663-667. FAN Ying,YU Xiaoqing,WANG Shuxian,et al. Application of different forms ofAstragalus polysaccharides in sea cucumber apostichopus japonicus aquaculture [J]. Fishery Science,2012,31(11): 663-667.
[14]	张海平. 复合山楂黄芪抗衰老保健饮料的研制及功能性研究[D]. 新乡: 河南科技学院,2012. ZHANG Haiping. The development and functional study of hawkthorn and Astragalus membranaceus compound resisting apolexis beverage [D]. Xinxiang: Henan University of Science and Technology,2012.
[15]	刘杨,包华音,王晓燕. 不同产地黄芪药材中毛蕊异黄酮葡萄糖苷的定量分析[J]. 现代中药研究与实践,2014,28(3): 8-10. LIU Yang,BAO Huayin,WANG Xiaoyan. Determination of Calycosin-7-Glucoside in Astragali Radix from different place by HPLC [J]. Research and Practice on Chinese Medicines,2014,28(3): 8-10.
[16]	刘晓庆,李军,薛恒跃,等.不同来源、不同等级黄芪饮片中毛蕊异黄酮葡萄糖苷含量分析[J]. 药物分析杂志,2013,33(5): 874-880.LIU Xiaoqing,LI Jun,XUE Hengyue,et al. Content analysis of calycosin glucoside in Astragalus slices of different sources and different grad [J]. Chinese Journal of Pharmaceutical Analysis,2013,33(5): 874-880.
[17]	田文仓,冯紫薇,石福娟,等.不同栽培方式产地蒙古黄芪中黄芪甲苷和总黄酮含量差异分析[J].中国现代中药,2020,20(4): 585-590, 602.TIAN Wencang,FENG Ziwei,SHI Fujuan,et al. Difference analysis of Astragaloside Ⅳ and total flavonoids content from Astragalus membranaceus var. mongolicus under different cultivation patterns and producing areas [J]. Modern Chinese Medicine, 2020, 20(4): 585-590, 602.
[18]	郭建富,陈勇,郝秀英.药食同源中草药黄芪在北疆地区产业发展展望[J].新疆农业科技,2018,(3):23-24.GUO Jianfu,CHEN Yong,HAO Xiuying. Prospects for the industrial development of medicinal and edible Chinese herbal medicine Astragalus in northern Xinjiang [J]. Xinjiang Agricultural Science and Technology, 2018,(3): 23-24.
[19]	汪喜生,许洲,饶姗姗. 土壤中硒元素在中草药中的富集[J]. 光谱实验室,2005,(3):625-628. WANG Xisheng,XU Zhou,RAO Shanshan. The biological accumulation of Se from Soil in Chinese traditional herbs [J]. Chinese Journal of Spectroscopy Laboratory,2005,(3): 625-628.
[20]	唐玉霞,王慧敏,刘巧玲,等.土壤和植物硒素研究综述[J].河北农业科学,2008,(5):43-45.TANG Yuxia,WANG Huimin,LIU Qiaoling,et al. Summary of selenium in soil and plant [J]. Journal of Hebei Agricultural Sciences,2008,(5): 43-45.
[21]	谢娟平. 安康富硒区域不同产地中药材中硒含量的分析与比较[J]. 陕西农业科学,2018,64(8):9-12. XIE Juanping. Comparison and analysis of Se contents in Chinese medical herbs from different places of origin in Se-rich soil of Ankang [J]. Shaanxi Journal of Agricultural Sciences,2018,64(8): 9-12.
[22]	王鹏,侯振安,冶军,等. 磷和不同价态硒对小麦硒吸收转运的影响[J]. 新疆农业科学,2018,55(1):33-43. WANG Peng,HOU Zhenan,YE Jun,et al. Effects of phosphorus and different valence selenium on the absorption and transport of selenium in wheat [J]. Xinjiang Agricultural Sciences,2018,55 (1): 33-43.
[23]	马麦艳,张相锋,马正海,等. Na2SeO3对药食用真菌蛹虫草子实体生长及功能成分腺苷、虫草素的影响[J]. 新疆农业科学,2019,56(8):1510-1517. MA Maiyan,ZHANG Xiangfeng,MA Zhenghai,et al. Effect of sodium selenite (Na2SeO3) on fruiting body growth, adenosine and cordycepin of functional components in medical edible fungus of cordyceps militaris [J]. Xinjiang Agricultural Sciences,2019,56(8): 1510-1517.
[24]	Ren D. Y.,Hu Y. Y.,Luo Y. Y.,et al. Selenium-containing polysaccharides from Ziyang green tea ameliorate high-fructose diet induced insulin resistance and hepatic oxidative stress in mice [J]. Food Function,2015,6(10): 3342-3350.
[25]	Wang Y. F.,Li Y. F.,Liu Y. Y.,et al. Extraction, characterization and antioxidant activities of Se-enriched tea polysaccharides [J]. International Journal of Biological Macromolecules,2015,(77): 76-84.
[26]	董卫华,昝玉玺,李贞霞.亚硒酸钠对枸杞总黄酮与枸杞多糖含量的影响[J].时珍国医国药,2013,24(9):2259-2260.DONG Weihua,ZAN Yuxi,LI Zhenxia.Effect of sodium selenite on the content of total flavonoids and polysaccharides in wolfberry [J]. Lishizhen Medicine and Materia Medica Research,2013,24(9): 2259-2260.
[27]	田秀英,王正银.硒对苦荞硒、总黄酮和芦丁含量、分布与累积的影响[J].植物营养与肥料学报,2008,(4):721-727.TIAN Xiuying,WANG Zhengyin. Effects of selenium application on content, distribution and accumulation of selenium, flavonoids and rutin in tartary buckwheat [J]. Plant Nutrition and Fertilizer Science,2008,(4) : 721-727.

不同产地黄芪活性成分与硒含量比较分析

Comparative Analysis of Active Ingredients and Selenium Contents in Astragalus from Different Habitats

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