中药复方多糖对不同MHC B-LβⅡ基因型蛋鸡育雏育成期免疫功能的影响

doi:10.6048/j.issn.1001-4330.2018.04.021

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (4): 763-773.DOI: 10.6048/j.issn.1001-4330.2018.04.021

中药复方多糖对不同MHC B-LβⅡ基因型蛋鸡育雏育成期免疫功能的影响

刘晓婷,罗燕,陈洁,连科讯,刘钢,马昭,朱晓庆,谷新利

石河子大学动物科技学院,新疆石河子 832003

收稿日期:2018-01-04 出版日期:2018-04-20 发布日期:2018-07-18
通信作者: 朱晓庆(1981-),女,甘肃酒泉人,讲师,博士,研究方向为中兽药制剂,(E-mail)zxhqqmy@126.com 谷新利(1963-),男,河南许昌人,博士,博士生导师,研究方向为中药制剂,(E-mail)xlgu@shzu.edu.cn
作者简介:刘晓婷(1991-),女,河南濮阳人,硕士研究生,研究方向为中草药制剂,(E-mail)1341280217@qq.com
基金资助:
国家自然科学基金项目“一定纯度中药复方多糖对不同MHC B-Lβ II基因型鸡免疫调节作用及其作用机制研究”(31460673)

Effects of Chinese Herbal Compound Polysaccharides on Immune Functions during the Brood Incubation Period in Different MHC B-Lβ II Genotype of Laying Hens

LIU Xiao-ting, LUO Yan, CHEN Jie, LIAN Ke-xun, LIU Gang, MA Zhao, ZHU Xiao-qing, GU Xin-li

College of Animal Science and Technology, Shihezi University, Shihezi Xinjiang 832003, China

Received:2018-01-04 Online:2018-04-20 Published:2018-07-18
Correspondence author: ZHU Xiao-qing (1981-), femal, native place: Jiuquan Gansu, lecturer,doctor, research field: research and development in traditional Chinese veterinary medicine preparation. (E-mail) zxhqqmy@126.com Gu Xin-li (1963-), male, native place: Xuchang Henan, doctoral tutor, doctor, research field: development and application of traditional Chinese medicine preparation. (E-mail)xlgu@shzu. edu.cn.
Supported by:
The National Natural Science Foundation of China "Study on immunomodulatory effect of polysaccharides with certain purity on chicken with different MHC B-L β II genotypes and its mechanism"（31460673）

摘要/Abstract

摘要： 【目的】研究肌注中药复方多糖对不同MHC B-Lβ II基因型蛋鸡免疫器官指数、血常规指标及血清中GM-CSF、G-CSF质量浓度的影响。【方法】采用PCR-SSCP方法,对试验鸡按不同基因型进行分组,将各组分成高、中、低剂量中药复方多糖试验组和空白对照组。各组试验鸡分别于8日龄肌肉注射0.2 mL 50、25和12.5 mg/mL中药复方多糖和生理盐水,连续7 d,于21、35和49日龄时从各组随机抽取5只鸡,测定胸腺、脾脏和法氏囊脏器指数,并采集血液检测血常规指标、粒细胞集落刺激因子(G-CSF)和粒-巨噬细胞集落刺激因子(GM-CSF)质量浓度的变化。【结果】不同基因型鸡中,高、中、低剂量多糖对免疫器官、血常规部分指标及GM-CSF均有一定影响,但各剂量多糖对各基因型同一指标的影响不相同。其中,AA基因型鸡中,高、中剂量中药复方多糖可显著提高胸腺、脾脏和法氏囊的脏器指数,低剂量中药复方多糖可显著提高其白细胞总数和淋巴细胞数;BB基因型鸡中,高、中剂量中药复方多糖可显著提高胸腺和脾脏指数,而对血常规指标无影响;BC基因型鸡中,高剂量中药复方多糖可显著提高胸腺指数、白细胞总数和淋巴细胞数,中剂量中药复方多糖可显著提高白细胞总数和淋巴细胞数。【结论】不同剂量的中药复方多糖可以不同程度的提高各基因型组蛋鸡免疫器官指数、部分血常规指数及GM-CSF质量浓度,以高、中剂量中药复方多糖免疫效果更好。

关键词: 中药复方多糖; MHC B-Lβ II基因型; 蛋鸡; 免疫器官指数; 血常规

Abstract: 【Objective】 This experiment aims to study the effects of intramuscular injection of compound polysaccharides on immune organs, blood routine indexes and the GM-CSF, G-CSF mass fraction in different MHC B-Lβ II genotype of laying hens.【Method】500 chickens were divided into three groups according to different genotypes by PCR-SSCP method. The genotypes were divided into high, medium and low dose Chinese herbal compound polysaccharide group and blank control group. Each group of the testing chickens were intramuscularly injected with 50 mg / mL, 25 mg / mL, 12.5 mg / mL Chinese herbal compound polysaccharide and saline, 0.2 mL / day for 7 days. At the age of 21, 35 and 49 days, 5 chickens were randomly selected from each group to measure the thymus, spleen and bursa of Fabricius viscera index, and blood samples were collected to measure the blood routine indexes and the changes of granulocyte colony stimulating factor G-CSF and granulocyte macrophage colony stimulating factor GM-CSF.【Result】The high, medium and low dose polysaccharides had certain effects on immune organs, blood routine indexes and GM-CSF in different genotype chickens, but the effects of different dosage polysaccharides on the same index of different genotypes were different. Among them, the high and medium doses of traditional Chinese medicine compound polysaccharide could significantly improve the organ index of thymus, spleen and bursa of fabricius in AA genotype chickens, and low dose of traditional Chinese medicine compound polysaccharide could significantly improve the total number of white blood cells and lymphocytes. In the BB genotype chickens, the high and medium dose of traditional Chinese medicine compound polysaccharide could significantly improve the thymus and spleen index, but had no effect on blood routine indexes. In the BC genotype chickens, high dose of traditional Chinese medicine compound polysaccharide could significantly improve the thymus index, white blood cell count and lymphocyte count, and the medium dose of traditional Chinese medicine compound polysaccharide could significantly improve the total white blood cell count and lymphocyte count.【Conclusion】Different doses of traditional Chinese medicine compound polysaccharide can improve the immune organ index, some blood routine index and GM-CSF mass concentration of hens in different genotype groups, and in general, the immune effect of Chinese herbal compound polysaccharide with high and medium doses is better.

Key words: Chinese medicine compound polysaccharide;MHC B-Lβ II genotype;laying hens; immune organs; blood routine indexes

中图分类号:

S853.72

刘晓婷,罗燕,陈洁,连科讯,刘钢,马昭,朱晓庆,谷新利. 中药复方多糖对不同MHC B-LβⅡ基因型蛋鸡育雏育成期免疫功能的影响[J]. 新疆农业科学, 2018, 55(4): 763-773.

LIU Xiao-ting, LUO Yan, CHEN Jie, LIAN Ke-xun, LIU Gang, MA Zhao, ZHU Xiao-qing, GU Xin-li. Effects of Chinese Herbal Compound Polysaccharides on Immune Functions during the Brood Incubation Period in Different MHC B-Lβ II Genotype of Laying Hens[J]. Xinjiang Agricultural Sciences, 2018, 55(4): 763-773.

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中药复方多糖对不同MHC B-LβⅡ基因型蛋鸡育雏育成期免疫功能的影响

Effects of Chinese Herbal Compound Polysaccharides on Immune Functions during the Brood Incubation Period in Different MHC B-Lβ II Genotype of Laying Hens

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