Hoxc13基因在苏博美利奴羊胎羔生长发育期不同组织中的表达分析

doi:10.6048/j.issn.1001-4330.2019.05.017

新疆农业科学 ›› 2019, Vol. 56 ›› Issue (5): 936-944.DOI: 10.6048/j.issn.1001-4330.2019.05.017

• 土壤肥料·贮藏加工·植物保护·畜牧水产 • 上一篇下一篇

Hoxc13基因在苏博美利奴羊胎羔生长发育期不同组织中的表达分析

杜建文¹, 何军敏¹, 陈春艳¹, 田月珍², 徐新明², 付雪峰², 哈尼克孜·吐拉甫²,
赵冰茹¹, 朱桦¹, 黄锡霞¹, 田可川²

1.新疆农业大学动物科学学院,乌鲁木齐,830052;
2.新疆畜牧科学院畜牧研究所,乌鲁木齐,830011

收稿日期:2019-04-10 发布日期:2019-05-20
通信作者: 黄锡霞(1963-),女,新疆人,教授,博士生导师,研究方向为动物遗传育种,(E-mail)1103335122@qq.com;
田可川(1963-),男,新疆人,研究员,博士生导师,研究方向为动物遗传育种,(E-mail)15909001963
作者简介:杜建文(1995-),女,新疆人,硕士研究生,研究方向为动物遗传育种,(E-mail)1103335122@qq.com
基金资助:
国家自然科学基金(31460593,3760655);国家绒毛用羊现代产业技术体系建设项目(CARS-40)

Expression Analysis of Hoxc13 Gene in Different Tissues of Subo Merino Sheep during Growth and Development

DU Jian-wen¹, HE Jun-min¹, CHEN Chun-yan¹, TIAN Yue-zhen², XU Xin-ming², FU Xue-feng², Hanikezi Tulafu ², ZHAO Bing-ru¹, ZHU Hua¹, HUANG Xi-xia¹ , TIAN Ke-chuan²

1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
2. Institute of Animal Science, Xinjiang Academy of Animal Sciences, Urumqi 830000, China

Received:2019-04-10 Published:2019-05-20
Correspondence author: HUANG Xi-xia(1963-), female, native place: Xinjiang, professor, PhD Tutor, research field: Animal genetic breeding, (E-mail)au-huangxixia@163.com
TIAN Ke-chuan(1963-), male, native place: Xinjiang, researcher, PhD Tutor, research field: Animal genetic breeding,(E-mail) tiankechuan@163.com
Supported by:
National Natural Science Foundation(No.31460593,No.3760655); National sheep & Goat modern industrial technology system construction project(CARS-40)

摘要/Abstract

摘要： 【目的】分析苏博美利奴羊不同组织中Hoxc13基因mRNA的表达量差异,为分子标记辅助育种以及,研究细毛羊主要经济性状,奠定理论基础。【方法】使用RT-PCR技术检测Hoxc13基因在苏博美利奴羊65和135 d皮肤、肌肉和不同内脏组织(心脏、脾脏、肝脏、肺脏、肾脏)中mRNA的表达量,并采用相对定量法对其表达量进行统计分析。【结果】在苏博美利奴羊皮肤、肌肉和不同内脏组织中均有Hoxc13基因的表达,并且在皮肤中的表达量显著高于其它组织(P<0.05),毛囊的形成和毛发生长与Hoxc13基因密切相关。【结论】建立了胎龄分别在65和135 d的苏博美利奴羊,其不同组织中适于定量检测的Hoxc13基因表达量的RT-PCR方法,从分子水平探讨了苏博美利奴羊的组织器官与Hoxc13基因表达之间的内在联系。

关键词: 苏博美利奴羊; RT-PCR; 组织; Hoxc13基因

Abstract: 【Objective】 The main purpose of this study is to lay a theoretical foundation for further development of molecular marker-assisted breeding of important economic traits of fine wool sheep by analyzing the expression difference of Hoxc13 gene mRNA in different tissues of Subo Merino sheep. 【Methods】In this study, RT-PCR was used to detect the mRNA expression of Hoxc13 gene in skin, muscle and different visceral tissues (heart, spleen, liver, lung, kidney) in Subo Merino for 65 days and 135 days, and the relative quantitative method was used to conduct the statistical analysis.【Results】The results showed that the Hoxc13 gene was expressed in skin and different tissues of Subo Merino sheep, and the expression level in the skin was significantly higher than that of other tissues(P<0.05).【Conclusion】This experiment establishes a RT-PCR method of the expression of Hoxc13 for detection of the gestational ages of 65 and 135 days of Subo Merino sheep in its different tissues in order to investigate the intrinsic link between the different tissues and the expression of Hoxc13 of Subo Merino sheep at the molecular level

Key words: Subo Merino sheep; RT-PCR; tissues; Hoxc13

中图分类号:

S826.8

杜建文, 何军敏, 陈春艳, 田月珍, 徐新明, 付雪峰, 哈尼克孜·吐拉甫,
赵冰茹, 朱桦, 黄锡霞, 田可川. Hoxc13基因在苏博美利奴羊胎羔生长发育期不同组织中的表达分析[J]. 新疆农业科学, 2019, 56(5): 936-944.

DU Jian-wen, HE Jun-min, CHEN Chun-yan, TIAN Yue-zhen, XU Xin-ming, FU Xue-feng, Hanikezi Tulafu , ZHAO Bing-ru, ZHU Hua, HUANG Xi-xia , TIAN Ke-chuan. Expression Analysis of Hoxc13 Gene in Different Tissues of Subo Merino Sheep during Growth and Development[J]. Xinjiang Agricultural Sciences, 2019, 56(5): 936-944.

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Hoxc13基因在苏博美利奴羊胎羔生长发育期不同组织中的表达分析

Expression Analysis of Hoxc13 Gene in Different Tissues of Subo Merino Sheep during Growth and Development

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