中国美利奴羊羊毛弯曲相关microRNA的筛选

doi:10.6048/j.issn.1001-4330.2021.03.021

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (3): 573-580.DOI: 10.6048/j.issn.1001-4330.2021.03.021

• 园艺特产·植物保护·畜牧兽医·微生物·农业生态环境 • 上一篇下一篇

中国美利奴羊羊毛弯曲相关microRNA的筛选

于丽娟, 张艳花, 拉扎特·艾尼瓦尔, 徐新明, 玛尔孜娅·亚森, 狄江

新疆畜牧科学院畜牧研究所,乌鲁木齐 830011

收稿日期:2020-08-03 出版日期:2021-03-20 发布日期:2021-03-30
通信作者: 狄江(1969-),男,新疆巴里坤人,研究员,博士,研究方向为动物遗传育种,(E-mail)dijiang69@163.com
作者简介:于丽娟(1986-),女,新疆博乐人,副研究员,硕士,研究方向为动物分子遗传,(E-mail)yljxinjiang120@sina.com
基金资助:
新疆维吾尔自治区自然科学基金(2017D01B37)

Screening microRNAs Related to Wool Crimp in Chinese Merino Sheep

YU Lijuan, ZHANG Yanhua, Lazate Ainiwaer, XU Xinming, Maerziya Yasen, DI Jiang

Institute of Animal Sciences, Xinjiang Academy of Animal Sciences,Urumqi 830011,China

Received:2020-08-03 Online:2021-03-20 Published:2021-03-30
Correspondence author: DI Jiang(1969- ),male, native place: Balikun, Xinjiang.Researcher,research filed: Animal genetics and breeding,(E-mail)dijiang69@163.com
Supported by:
the Natural Science Foundation of Xinjiang Uygur Autonomous Region (2017D01B37)

摘要/Abstract

摘要： 【目的】 研究miRNA在不同羊毛弯曲中国美利奴羊皮肤组织中的表达差异及其可能参与的调控通路,筛选出与中国美利奴羊羊毛弯曲相关的miRNA,为进一步探究细毛羊羊毛弯曲性状的调控机理提供理论依据。【方法】 运用高通量测序技术对羊毛弯曲频率有极端差异表型值(大弯曲组与小弯曲组)细毛羊个体皮肤的miRNA组进行测序与比较分析。【结果】 共鉴定出425个miRNAs,其中包括143个已知miRNAs和282个新发现的miRNAs。在大弯曲组与小弯曲组共筛选到8个上调和17个下调的miRNAs。对差异miRNA的预测靶基因进行简单的Gene Ontology与KEGG Pathway富集分析。差异表达的miRNA的靶基因主要参与跨膜信号受体活动、信号转导活动、分子转导活动、膜的组成等过程。15 761个靶基因注释到252个信号通路。【结论】 筛选出了25个与细毛羊羊毛弯曲性状相关的候选miRNAs。

关键词: 中国美利奴羊; 羊毛弯曲; 皮肤组织; miRNA

Abstract: 【Objective】 To explore the role of miRNAs at post-transcriptional levels in skin tissues of different wool crimp and possible regulatory pathways.【Method】 High-throughput sequencing technology was used to compare and analyze the differential expression of miRNA in the skin tissue of fine wool sheep with extremely different wool staple crimp frequency (big crimp group and small crimp group).【Result】 A total of 424 miRNAs were identified, including 143 known and 282 newly discovered miRNAs. Difference analysis of the identified miRNAs revealed that 8 up-regulated miRNAs and 17 down-regulated miRNAs were screened in big crimp group and small crimp group. According to the corresponding relationship between miRNA and its target genes, simple Gene Ontology and KEGG Pathway enrichment analysis was performed on the target genes of differentially expressed miRNA. GO statistics demonstrated that the target genes were mainly involved in transmembrane signaling receptor activity, signal transducer activity, molecular transducer activity, integral component of membrane and other processes; and KEGG pathway analysis showed that 15,761 target genes were enriched in 252 signaling pathways.【Conclusion】 In this study, 25 candidate miRNAs related to wool crimp of fine wool sheep were screened out, which will provid a theoretical basis for further research on the regulatory mechanism of wool crimp.

Key words: Chinese Merino sheep; wool crimp; skin tissue; miRNA

中图分类号:

S826

于丽娟, 张艳花, 拉扎特·艾尼瓦尔, 徐新明, 玛尔孜娅·亚森, 狄江. 中国美利奴羊羊毛弯曲相关microRNA的筛选[J]. 新疆农业科学, 2021, 58(3): 573-580.

YU Lijuan, ZHANG Yanhua, Lazate Ainiwaer, XU Xinming, Maerziya Yasen, DI Jiang. Screening microRNAs Related to Wool Crimp in Chinese Merino Sheep[J]. Xinjiang Agricultural Sciences, 2021, 58(3): 573-580.

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中国美利奴羊羊毛弯曲相关microRNA的筛选

Screening microRNAs Related to Wool Crimp in Chinese Merino Sheep

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