黑曲霉ZD利用棉花秸秆固体发酵产纤维素酶条件优化

doi:10.6048/j.issn.1001-4330.2018.09.018

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (9): 1717-1727.DOI: 10.6048/j.issn.1001-4330.2018.09.018

• 植物保护·土壤肥料·农业生态环境 • 上一篇下一篇

黑曲霉ZD利用棉花秸秆固体发酵产纤维素酶条件优化

郭凯^1,3, 崔卫东¹, 王宁¹, 侯敏¹, 包慧芳¹, 房世杰²

1.新疆农业科学院微生物应用研究所,乌鲁木齐 830091;
2.新疆农业科学院科研管理处,乌鲁木齐 830091;
3.新疆大学生命科学与技术学院,乌鲁木齐 830052

收稿日期:2018-08-09 出版日期:2018-09-20 发布日期:2018-12-07
通信作者: 房世杰(1982-),男,副研究员,硕士,研究方向为发酵工程,(E-mail)shwnfang2010@sina.cn
作者简介:郭凯(1993-),男,硕士研究生,研究方向为微生物发酵工程,(E-mail)guokairn@sohu.com
基金资助:
新疆维吾尔自治区青年基金项目“木质纤维素降解菌株选育及混菌发酵在棉秆饲料中降解效果研究”(2015211B019);自治区公益性科研院所基本科研业务经费资助项目“棉秸秆饲料化微生物处理技术研究”(KY2014028)

Optimization of cellulase production by Aspergillus niger ZD using solid fermentation of cotton straw

GUO Kai^1,3, CUI Wei-dong¹, WANG Ning¹, HOU Min¹, BAO Hui-fang¹, FANG Shi-jie²

1.Research Institute of Applied Microbiology / Xin Jiang Academy of Agriculture Sciences.
2.Scientific R esearch Management Office,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;
3.College of Life Sciences and Technology,Xinjiang University, Urumqi 830052,China

Received:2018-08-09 Online:2018-09-20 Published:2018-12-07
Correspondence author: FANG Shi-jie(1982-),Associate researcher,master's degree,Research direction fermentation engineering
Supported by:
Xinjiang Uygur Autonomous Region Youth Fund Project "Study on the Degradation Effect of Lignocellulose Degrading Strains and Mixed Fermentation Fermentation in Cotton Stalk Feed"(2015211B019);Autonomous Region Research Center for Public Welfare Research Institutes Funded Project "Study on Microbial Treatment Technology of Cotton Straw Feeding"(KY2014028)

摘要/Abstract

摘要： 【目的】以棉花秸秆作为发酵基质,优化黑曲霉(Aspergillus niger)ZD固体发酵产纤维素酶的发酵条件。【方法】以固体发酵条件(棉花秸秆和玉米粉的比例、发酵时间、接种率、含水量和pH值)为优化因素,通过单因素试验和Plackett-Burman试验,确定主要影响因素;再由最陡爬坡试验确定优化中心点,最后通过 Box-Behnken中心组合设计确定主要影响因素及其交互作用对固体发酵产酶的影响,并对其进行回归分析。【结果】由单因素试验和Plackett-Burman试验确定碳氮比,接种量和含水率为主要影响因素;最陡爬坡试验确定棉花秸秆与玉米粉的比例4∶1,接种量10%和含水率60%为中心点;Box-Behnken中心组合试验确定最佳发酵条件:棉花秸秆和玉米粉的比例为4.4∶1,接种量8.12%,含水率60%,发酵时间40 d,pH为4,此条件下羧甲基纤维素钠酶活最高,为310.885 U/mL;比未优化条件下的酶活207.496 U/mL提高了49.8%。【结论】确定黑曲霉ZD利用棉花秸秆固体发酵产纤维素酶的最优条件,为利用该菌制备棉花秸秆发酵饲料,降解棉花秸秆纤维素含量,提供了理论依据和工艺参数。

关键词: 黑曲霉; 棉花秸秆; 响应面; 固体发酵

Abstract: 【Objective】 Optimization of cellulase production by Aspergillus niger ZD using solid fermentation, using cotton straw as a fermentation substrate.【Method】Identify the main influencing factors by single factor test and Plackett-Burman test, as optimization factor of the ratio of cotton straw and corn flour, fermtation time, inoculation rate, water content and pH value under solid fermentation conditions. Then determines the optimization center point through the steepest climbing test, finally the influence of main influencing factors and their interactions on enzyme production was determined by Box-Behnken center combination design under solid fermentation, and regression analysis of the main influences.【Result】Identify the main influences of the ratio of cotton straw and corn flour, inoculation rate and water content by single factor test and Plackett-Burman test; dedetermines the optimization center point of 4∶1 of cotton straw-corn flour ratio, 10% of inoculation rate, 60% of water content; the optimal fermentation conditions was 4.4∶1 of cotton straw-corn flour ratio, 8.12% of inoculation rate, 65% of water content by Box-Behnken center combination tests.The maximum carboxymethylcellulose sodium enzyme activity under this condition was 310.885 U/mL, which was 49.8% higher than the enzyme activity 207.496 U/mL under unoptimized conditions. 【Conclusion】In conclusion, the optimal fermentation conditions of cellulase production by Aspergillus niger ZD using cotton straw solid fermentation was obtain. The results can be used to make cotton straw fermented feed using bacteria mixed, to degradate cellulose content of cotton straw and provide theoretical basis and process parameters.

Key words: Aspergillus niger; cotton straw; solid fermentation; response surface method ; ;

中图分类号:

Q815
S562

郭凯, 崔卫东, 王宁, 侯敏, 包慧芳, 房世杰. 黑曲霉ZD利用棉花秸秆固体发酵产纤维素酶条件优化[J]. 新疆农业科学, 2018, 55(9): 1717-1727.

GUO Kai, CUI Wei-dong, WANG Ning, HOU Min, BAO Hui-fang, FANG Shi-jie. Optimization of cellulase production by Aspergillus niger ZD using solid fermentation of cotton straw[J]. Xinjiang Agricultural Sciences, 2018, 55(9): 1717-1727.

参考文献

[1] 龚安家.棉花秸秆饲料的开发与利用[J].农村科技,2007,(6):73-74.
   GONG An-jia.(2007). Development and Utilization of Cotton Straw Feed[J]. Rural Science and Technology,(6):73-74. (in Chinese)
[2]高瑞芳,张吉树.新疆棉花秸秆饲料化开发利用研究[J].中国畜牧杂志,2016,52(8):76-80.
   GAO Rui-fang, ZHANG Ji-shu. (2016). Study on the Development and Utilization of Cotton Straw Feed in Xinjiang[J]. Chinese Journal of Animal Science,52(8):76-80. (in Chinese)
[3]王双磊,李金埔,赵洪亮,等.棉花秸秆利用现状与还田潜力分析研究[J].山东农业大学学报(自然科学版),2014,45(2):310-315.
   WANG Shuang-lei, LI Jin-pu, ZHAO Hong-liang,et al. (2014). Analysis of the Utilization Status of Cotton Straw and the Potential of Returning Field[J]. Journal of Shandong Agricultural University,45(2):310-315. (in Chinese)
[4]张福元,宋燕青,程文晓,等.黑曲霉发酵玉米秸秆产纤维素酶的研究[J].山西农业大学学报(自然科学版),2009,29(3):206-210.
   ZHANG Fu-yuan, SONG Yan-qing, CHENG Wen-xiao,et al. (2009). Study on cellulase production by fermentation of corn stover by Aspergillus niger[J]. Journal of Shanxi Agricultural University,29(3):206-210. (in Chinese)
[5]伍红,陆兆新,吕玫,等.黑曲霉AF-98固体发酵产纤维素酶的产酶条件研究[J].菌物学报,2006,(3):475-480.
   WU Hong, LU Zhao-xin, LÜ Mei, et al. (2006). Study on the enzyme production conditions of cellulase produced by Aspergillus niger AF-98 solid fermentation[J]. Journal of Fungal Science,(3):475-480. (in Chinese)
[6]张宁,蒋剑春,杨静,等.应用里氏木霉发酵制备纤维素酶固体曲的研究[J].生物质化学工程,2014,48(5):7-10.
   ZHANG Ning, JIANG Jian-chun, YANG Jing, et al. (2014). Study on the Preparation of Cellulase Solids by Fermentation of Trichoderma Reesei[J]. Biomass Chemical Engineering,48(5):7-10. (in Chinese)
[7]曲丽丽. 海洋黑曲霉固态发酵耐盐纤维素酶的培养条件优化及粗酶制剂的制备[D].浙江大学,2017.
   QU Li-li. (2017).Optimization of culture conditions of solid fermentation of salt-tolerant cellulase by Aspergillus niger and preparation of crude enzyme preparation[D]. Zhejiang University. (in Chinese)
[8]严烨,杨绍青,范光森,等.樟绒枝霉(Malbranchea cinnamomea)固体发酵产木聚糖酶的发酵条件优化[J].微生物学通报,2013,40(8):1 339-1 346.
   YAN Yan, YANG Shao-qing, FAN Guang-sen, et al. (2013). Optimization of Fermentation Conditions for Producing Xylanase by Solid Fermentation of Malbranchea cinnamomea[J]. Microbiology,40(8):1,339-1,346. (in Chinese)
[9]訾慧. 青霉ZH1产纤维素酶条件的优化及其酶学性质研究[D].沈阳农业大学,2017.
   QI Hui. (2017). Optimization of Cellulase Production Conditions and Enzymatic Properties of Penicillium ZH1[D]. Shenyang Agricultural University. (in Chinese)
[10]黄晓梅,赵红晓,范金霞,等.一株高产纤维素酶绿色木霉菌株诱变选育与发酵研究[J].东北农业大学学报,2018,49(6):22-31.
   HUANG Xiao-mei, ZHAO Hong-xiao, FAN Jin-xia,et al.(2018). Study on Mutation Breeding and Fermentation of a High-yield Cellulase Trichoderma Strains[J]. Journal of Northeast Agricultural University,49(6):22-31. (in Chinese)
[11]赵旭,王文丽,李娟.1株产高温纤维素酶细菌的分离及Biolog鉴定[J].甘肃农业科技,2018,(6):30-33.
   ZHAO Xu, WANG Wen-li, LI Juan.(2018). Isolation and Biolog Identification of a High Temperature Cellulase Producing Bacterium[J]. Gansu Agricultural Science and Technology,(6):30-33. (in Chinese)
[12]蒋明星,丁晓帆.纤维素降解细菌的筛选及其酶活测定[J].中国农学通报,2015,31(36):161-164.
   JIANG Ming-xing, DING Xiao-fan.(2015). Screening of Cellulose-Degrading Bacteria and Its Enzyme Activity Measurement[J]. Chinese Agricultural Science Bulletin,31(36):161-164. (in Chinese)
[13]邹水洋,郭祀远.空白实验及测试条件对纤维素酶活测定的影响[J].食品工业科技,2010,31(6):344-346.
   ZOU Shui-yang,GUO Yu-yuan. (2010). Effect of blank experiment and test conditions on cellulase activity determination[J]. Science and Technology of Food Industry,31(6):344-346. (in Chinese)
[14]宋晓妍,孙彩云,张桂香,等.绿色木霉Tr-A1固体发酵生产纤维素酶[J].济南大学学报(自然科学版),2011,25(3):265-268.
   SONG Xiao-yan, SUN Cai-yun, ZHANG Gui-xiang, et al. (2011). Study on cellulase production by solid fermentation of Trichoderma viride Tr-A1[J]. Journal of Jinan University(Natural Science Edition) ,25(3):265-268. (in Chinese)
[15]吴发远.康氏木霉固体发酵生产纤维素酶条件的研究[J].中国西部科技,2011,10(23):1-3.
   WU Fa-yuan. (2011). Study on the Conditions of Cellulase Production by Solid Fermentation of Trichoderma koningii[J]. Western Science and Technology,10(23):1-3. (in Chinese)
[16]林蒙蒙. 白腐真菌Inonotus obliquus产生纤维素酶的发酵工艺及木质纤维素糖化研究[D].浙江理工大学,2018.
   LIN Meng-meng. (2018). Cellulase fermentation process and lignocellulose saccharification of white rot fungus Inonotus obliquus[D]. Zhejiang University of Science and Technology. (in Chinese)
[17]靖德兵,李培军,王力华,等.康氏木霉固体发酵生产纤维素酶优化研究-培养基用料与工艺条件优化[J].食品科学,2004,(5):82-86.
   JING De-bing, LI Pei-jun, WANG Li-hua, et al. (2004). Optimization of Cellulase Production by Solid Fermentation of Trichoderma koningii-Optimization of Medium Materials and Process Conditions[J]. Food Science,(5):82-86. (in Chinese)
[18]袁丽环.固体发酵玉米秸秆产纤维素酶条件的优化[J].陕西农业科学,2011,57(2):13-14, 47.
   YUAN Li-huan.(2011). Optimization of Cellulase Production Conditions in Solid Fermented Corn Stalks[J]. Shaanxi Agricultural Sciences,57(2):13-14, 47. (in Chinese)
[19]张辉,桑青,马艳霞.嗜热侧孢霉固体发酵产纤维素酶条件研究[J].食品研究与开发,2008,(1):4-7.
   ZHANG Hui, SANG Qing, MA Yan-xia.(2008). Study on the Conditions of Cellulase Production by Solid Fermentation of Thermomyces faecalis[J]. Food Research and Development,(1):4-7. (in Chinese)

黑曲霉ZD利用棉花秸秆固体发酵产纤维素酶条件优化

Optimization of cellulase production by Aspergillus niger ZD using solid fermentation of cotton straw

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