新疆农业科学 ›› 2023, Vol. 60 ›› Issue (6): 1423-1432.DOI: 10.6048/j.issn.1001-4330.2023.06.015
李佩琪1(), 孙庆培1, 王志慧2, 秦新政2(), 樊永红1()
收稿日期:
2022-09-29
出版日期:
2023-06-20
发布日期:
2023-06-20
通信作者:
秦新政(1977-),新疆石河子人,副研究员,硕士,研究方向为环境微生物学,(E-mail) qinxinzheng@163.com; 樊永红(1974-),女,甘肃静宁人,教授,博士,硕士生导师,研究方向为环境微生物学,(E-mail) yhfanzyb2004@163.com
作者简介:
李佩琪(1997-),女,硕士研究生,研究方向为环境微生物学,(E-mail)2394767902@qq.com
基金资助:
LI Peiqi1(), SUN Qingpei1, WANG Zhihui2, QIN Xinzheng2(), FAN Yonghong1()
Received:
2022-09-29
Online:
2023-06-20
Published:
2023-06-20
Correspondence author:
QIN Xinzheng(1977-), male, native place: Shihezi, Xinjiang. Associate researcher. Research field: Environmental microbiology, (E-mail) qinxinzheng@163.com; FAN Yonghong(1974-), female, native place: Jinning, Gansu. Professor. Research field: Environmental microbiology, (E-mail) yhfanzyb2004@163.com
Supported by:
摘要:
【目的】分析棉花秸秆经固体发酵转化为有机基质过程中的木质素降解与木质素降解酶活性的关联性,为研究木质素降解机理奠定基础。【方法】对棉秆进行固体发酵,测定温度、纤维素及半纤维素含量,利用紫外检测和高温灼烧法分别测定发酵过程中酸溶性木质素和酸不溶性木质素的含量变化以及3种主要的木质素降解酶的活性。【结果】在棉秆固体发酵过程中,棉秆木质素含量由31.51%显著降低至25.38%(P<0.05),木质素降解速率在高温期后显著升高,在熟化期时持续升高,直至发酵结束时达到最高,为13.84 g/d。木质素降解酶活性在熟化期时显著升高,在发酵结束时达到最高值,其中锰过氧化物酶活性12.91 U/g,木质素过氧化物酶活性55.28 U/g,漆酶活性0.19 U/g。木质素降解酶活性和木质素降解速率呈显著正相关(P<0.01),3种木质素降解酶活性之间也呈显著正相关(P<0.01);锰过氧化物酶在棉秆固体发酵的木质素降解中起到主要作用。【结论】棉秆固体发酵过程中木质素降解的规律及其与木质素降解酶活性的关联。
中图分类号:
李佩琪, 孙庆培, 王志慧, 秦新政, 樊永红. 棉秆固体发酵中木质素降解与酶活性变化的关联分析[J]. 新疆农业科学, 2023, 60(6): 1423-1432.
LI Peiqi, SUN Qingpei, WANG Zhihui, QIN Xinzheng, FAN Yonghong. Correlation analysis of lignin degradation and enzyme activity changes in solid fermentation of cotton stalks[J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1423-1432.
固体发酵时期 Solid fermentation period | 失重率 Weight loss rate (%) | 纤维素含量 Cellulose content (%) | 半纤维素含量 Hemicellulose content (%) | 木质素含量 Lignin content (%) |
---|---|---|---|---|
初始 Initial stage | 0.00a | 29.32±1.81a | 15.17±0.71a | 31.51±0.98a |
升温期 Warming period | 5.86±1.05b | 28.47±1.49a | 13.30±0.67b | 31.45±0.88a |
高温期 High temperature period | 6.42±0.9b | 28.36±0.87a | 11.48±0.16c | 30.60±1.19ab |
熟化期 Maturation period | 29.30±0.75c | 21.27±0.38b | 8.42±0.15d | 27.52±1.25ab |
末期 Final stage | 35.59±1.55d | 18.18±0.82c | 7.75±0.34d | 25.38±0.98b |
表1 固体发酵棉秆中纤维素、半纤维素和木质素含量
Tab.1 Contents of cellulose, hemicellulose and lignin in solid fermented cotton straw
固体发酵时期 Solid fermentation period | 失重率 Weight loss rate (%) | 纤维素含量 Cellulose content (%) | 半纤维素含量 Hemicellulose content (%) | 木质素含量 Lignin content (%) |
---|---|---|---|---|
初始 Initial stage | 0.00a | 29.32±1.81a | 15.17±0.71a | 31.51±0.98a |
升温期 Warming period | 5.86±1.05b | 28.47±1.49a | 13.30±0.67b | 31.45±0.88a |
高温期 High temperature period | 6.42±0.9b | 28.36±0.87a | 11.48±0.16c | 30.60±1.19ab |
熟化期 Maturation period | 29.30±0.75c | 21.27±0.38b | 8.42±0.15d | 27.52±1.25ab |
末期 Final stage | 35.59±1.55d | 18.18±0.82c | 7.75±0.34d | 25.38±0.98b |
图2 棉秆发酵过程中木质纤维素降解速率变化 注:IS:初始;WP:升温期:HP:高温期;MP:熟化期;FS:末期,下同
Fig.2 Change of lignocellulose degradation rate during cotton stalk fermentation Note: IS: Initial stage;WP:Warming period:HP:High temperature period;MP:Maturation period;FS:Final stage, the same as below
图6 木质素降解酶活性及木质素降解速率曲线 注:A锰过氧化物酶;B木质素过氧化物酶;C漆酶
Fig.6 Lignin degradation enzyme activity and lignin degradation rate curve Note: A Manganese peroxidase; B Lignin peroxidase; C Laccase
指标 Index | 发酵温度 Fermentation temperature | Lac活性 Lac activity | LiP活性 LiP activity | MnP活 MnP activity | 木质素 降解速率 Lignin degradation rate | 纤维素降解速 Cellulose degradation rate | 半纤维素 降解速率 Hemicellulose degradation rate |
---|---|---|---|---|---|---|---|
发酵温度 Fermentation temperature | 1 | ||||||
Lac活性Lac activity | -0.461 | 1 | |||||
LiP活性LiP activity | -0.29 | 0.848** | 1 | ||||
MnP活性MnP activity | -0.281 | 0.888** | 0.908** | 1 | |||
木质素降解速率 Lignin degradation rate | -0.088 | 0.611** | 0.698** | 0.695** | 1 | ||
纤维素降解速率 Cellulose degradation rate | 0.344 | 0.467* | 0.477* | 0.617** | 0.666** | 1 | |
半纤维素降解速率 Hemicellulose degradation rate | 0.919** | -0.363 | -0.205 | -0.164 | -0.049 | 0.403 | 1 |
表2 木质素降解酶活性变化与木质素降解相关性
Tab.2 Correlation analysis between lignin degradation enzyme activity and lignin degradation
指标 Index | 发酵温度 Fermentation temperature | Lac活性 Lac activity | LiP活性 LiP activity | MnP活 MnP activity | 木质素 降解速率 Lignin degradation rate | 纤维素降解速 Cellulose degradation rate | 半纤维素 降解速率 Hemicellulose degradation rate |
---|---|---|---|---|---|---|---|
发酵温度 Fermentation temperature | 1 | ||||||
Lac活性Lac activity | -0.461 | 1 | |||||
LiP活性LiP activity | -0.29 | 0.848** | 1 | ||||
MnP活性MnP activity | -0.281 | 0.888** | 0.908** | 1 | |||
木质素降解速率 Lignin degradation rate | -0.088 | 0.611** | 0.698** | 0.695** | 1 | ||
纤维素降解速率 Cellulose degradation rate | 0.344 | 0.467* | 0.477* | 0.617** | 0.666** | 1 | |
半纤维素降解速率 Hemicellulose degradation rate | 0.919** | -0.363 | -0.205 | -0.164 | -0.049 | 0.403 | 1 |
成分 Components | 初始特征值 Initial eigenvalue | 提取平方和载入 Extraction sums of squared loadings | ||||
---|---|---|---|---|---|---|
特征值 Eigenvalue | 方差贡献率 Variance contribution rate (%) | 累积方差贡献率 Cumulative variance contribution rate (%) | 特征值 Eigenvalue | 方差贡献率 Variance contribution rate (%) | 累积方差贡献率 Cumulative variance contribution rate (%) | |
1 | 6.259 | 62.591 | 62.591 | 6.259 | 62.591 | 62.591 |
2 | 2.280 | 22.800 | 85.391 | 2.280 | 22.800 | 85.391 |
3 | 0.501 | 5.013 | 90.404 | |||
4 | 0.369 | 3.687 | 94.091 | |||
5 | 0.276 | 2.760 | 96.851 | |||
6 | 0.110 | 1.101 | 97.952 | |||
7 | 0.087 | 0.874 | 98.826 | |||
8 | 0.073 | 0.730 | 99.555 | |||
9 | 0.035 | 0.352 | 99.907 | |||
10 | 0.009 | 0.093 | 100.000 |
表3 总方差的解释
Tab.3 Interpretation of total variance
成分 Components | 初始特征值 Initial eigenvalue | 提取平方和载入 Extraction sums of squared loadings | ||||
---|---|---|---|---|---|---|
特征值 Eigenvalue | 方差贡献率 Variance contribution rate (%) | 累积方差贡献率 Cumulative variance contribution rate (%) | 特征值 Eigenvalue | 方差贡献率 Variance contribution rate (%) | 累积方差贡献率 Cumulative variance contribution rate (%) | |
1 | 6.259 | 62.591 | 62.591 | 6.259 | 62.591 | 62.591 |
2 | 2.280 | 22.800 | 85.391 | 2.280 | 22.800 | 85.391 |
3 | 0.501 | 5.013 | 90.404 | |||
4 | 0.369 | 3.687 | 94.091 | |||
5 | 0.276 | 2.760 | 96.851 | |||
6 | 0.110 | 1.101 | 97.952 | |||
7 | 0.087 | 0.874 | 98.826 | |||
8 | 0.073 | 0.730 | 99.555 | |||
9 | 0.035 | 0.352 | 99.907 | |||
10 | 0.009 | 0.093 | 100.000 |
指标 Index | 成分 Components | |
---|---|---|
1 | 2 | |
纤维素含量Cellulose content | -0.959 | 0.145 |
锰过氧化物酶活性Manganese peroxidase activity | 0.957 | 0.070 |
半纤维素含量Hemicellulose content | -0.955 | -0.187 |
木质素过氧化物酶活性Lignin peroxidases activity | 0.931 | -0.155 |
漆酶活性Laccase activity | 0.909 | 0.007 |
木质素降解速率Lignin degradation rate | 0.790 | 0.260 |
木质素含量Lignin content | -0.775 | 0.026 |
半纤维素降解速率Hemicellulose degradation rate | -0.278 | 0.918 |
温度Temperature | -0.362 | 0.898 |
纤维素降解速率Cellulose degradation rate | 0.621 | 0.690 |
表4 主成分因子负荷矩阵
Tab.4 Principal component factor loading matrix
指标 Index | 成分 Components | |
---|---|---|
1 | 2 | |
纤维素含量Cellulose content | -0.959 | 0.145 |
锰过氧化物酶活性Manganese peroxidase activity | 0.957 | 0.070 |
半纤维素含量Hemicellulose content | -0.955 | -0.187 |
木质素过氧化物酶活性Lignin peroxidases activity | 0.931 | -0.155 |
漆酶活性Laccase activity | 0.909 | 0.007 |
木质素降解速率Lignin degradation rate | 0.790 | 0.260 |
木质素含量Lignin content | -0.775 | 0.026 |
半纤维素降解速率Hemicellulose degradation rate | -0.278 | 0.918 |
温度Temperature | -0.362 | 0.898 |
纤维素降解速率Cellulose degradation rate | 0.621 | 0.690 |
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