Isolation,identification and pathogenicity of fungi on the surface of pomegranate fruit

doi:10.6048/j.issn.1001-4330.2024.01.016

Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (1): 148-155.DOI: 10.6048/j.issn.1001-4330.2024.01.016

• Agricultural Product Analysis and Detection·Forestry·Agricultural Information·Plant Protection • Previous Articles Next Articles

Isolation,identification and pathogenicity of fungi on the surface of pomegranate fruit

YU Lyujian¹^,², YANG Jin¹^,², DING Yu¹^,², LI Xiaoman³, LIU Fengjuan², FAN Yingying², LIANG Hongyu³, JIAO Ziwei¹(), WANG Cheng²()

1. College of Biological and Geographical Sciences,Yili Normal University,Yining Xinjiang 835000,China
2. Institute of Quality Standards & Testing Technology for Agro-Products,Xinjiang Academy of Agricultural Sciences / Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region(Co-Construction by Ministry and Province),Ministry of Agriculture and Rural Affairs/ Laboratory of Quality and Safety Risk Assessment for Agro-Products(Urumqi),Ministry of Agriculture and Rural Affairs / Key Laboratory of Agro-products Quality and Safety of Xinjiang,Urumqi 830091,China
3. College of Food Sciences and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China

Received:2023-05-11 Online:2024-01-20 Published:2024-02-21
Correspondence author: JIAO Ziwei(1973-),male,born in Henan Province,professor,PhD,research direction:microbial ecology and pest control in green organic agriculture,(E-mail)jiaziwei123@163.com; WANG Cheng(1971-),male,born in Shanxi,researcher,PhD,research direction:safety and quality of agricultural products,(E-mail)wangchengxj321@sina.com
Supported by:
Major S & T Research project of Xinjiang Uygur Autonomous Region(2021A02002-4);Yili Normal University High-level Talent Program(YLSDXSDTR22001)

石榴果实表面真菌的分离鉴定及致病性测定

于吕健¹^,², 阳瑾¹^,², 丁宇¹^,², 李晓曼³, 刘峰娟², 范盈盈², 梁红玉³, 焦子伟¹(), 王成²()

1.伊犁师范大学生物与地理科学学院,新疆伊宁 835000
2.新疆农业科学院农业质量标准与检测技术研究所/农业农村部荒漠绿洲生态区特色农产品功能营养与健康重点实验室(部省共建)/农业农村部农产品质量安全风险评估实验室(乌鲁木齐)/新疆农产品质量安全实验室,乌鲁木齐 830091
3.新疆农业大学食品科学与药学学院,乌鲁木齐 830052

通讯作者: 焦子伟(1973-),男,河南人,教授,博士,研究方向为微生物生态及绿色有机农业有害生物防控,(E-mail)jiaziwei123@163.com; 王成(1971-),男,陕西人,研究员,博士,研究方向为农产品质量安全,(E-mail)wangchengxi321@sina.com
作者简介:于吕健(1996-),男,黑龙江人,硕士研究生,研究方向为微生物病害防治,(E-mail)ylj2065@sina.com
基金资助:
新疆维吾尔自治区重大科技专项计划项目(2021A02002-4);伊犁师范大学“学实高层次人才岗位”项目(YLSDXSDTR22001)

Abstract

Abstract:

【Objective】 To preliminarily explore the fungal species on the surface of Kashgar pomegranate fruits and test their pathogenic ability.This finding has provided data reference for the prevention and control of fungal diseases in pomegranate fruit in Xinjiang. 【Methods】 The fungi on the surface of pomegranate fruit were isolated,purified,identified and classified by morphology and molecular biology,and then the pathogenicity of fungi to pomegranate fruit was determined by puncture inoculation test. 【Results】 Seven different fungi were isolated and identified,among which,the following four could obviously cause pomegranate fruit rot. Both of Aspergillus luchuensis and Penicillium italicum showed higher infection ability to pomegranate peel and grain; Alternaria eichhorniae only had higher infection ability to pomegranate pulp; Penicillium adametzioid had weaker infection ability to pomegranate peel and grain. In addition,Talaromyes rubrifaciens,Cladosporium antarctica and Amorphotheca resinae did not show obvious pathogenicity to peel and grain. 【Conclusion】 The main pathogenic bacteria of pomegranate fruit under the ecological conditions in southern Xinjiang are different from those in other suitable growing habitats in China.

Key words: pomegranate; microorganism; fungal diseases; isolation and identification; pathogenicity

摘要：

【目的】研究新疆喀什石榴果实表面真菌的种类,并测试其对石榴果实的致病能力。【方法】分离纯化石榴果实表面真菌,通过形态学和分子生物学对真菌进行鉴定分类,并通过刺伤接种试验测定真菌对石榴果实的致病性,为新疆石榴果实真菌病害的预防与治理提供数据参考。【结果】分离鉴定了7株不同的石榴表面真菌,其中4株可明显致石榴果实腐烂:琉球曲霉(Aspergillus luchuensis)和意大利青霉(Penicillium italicum)对石榴果皮和籽粒均表现出较高的侵染能力,水葫芦链格孢(Alternaria eichhorniae)仅对籽粒果肉具有较高侵染能力,阿氏青霉(Penicillium adametzioide)对石榴果皮和籽粒侵染能力较弱。红色篮状菌(Talaromyes rubrifaciens)、南极枝孢菌(Cladosporium antarcticum)和煤油霉菌(Amorphotheca resinae)未表现出对果皮和籽粒明显的致病能力。【结论】新疆南疆生态条件下石榴果实主要致病菌较其他适生地存在一定区别。

关键词: 石榴, 微生物, 真菌病害, 分离鉴定, 致病性

CLC Number:

S665.4

YU Lyujian, YANG Jin, DING Yu, LI Xiaoman, LIU Fengjuan, FAN Yingying, LIANG Hongyu, JIAO Ziwei, WANG Cheng. Isolation,identification and pathogenicity of fungi on the surface of pomegranate fruit[J]. Xinjiang Agricultural Sciences, 2024, 61(1): 148-155.

于吕健, 阳瑾, 丁宇, 李晓曼, 刘峰娟, 范盈盈, 梁红玉, 焦子伟, 王成. 石榴果实表面真菌的分离鉴定及致病性测定[J]. 新疆农业科学, 2024, 61(1): 148-155.

Figures/Tables 3

References 20

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Isolation,identification and pathogenicity of fungi on the surface of pomegranate fruit

石榴果实表面真菌的分离鉴定及致病性测定

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