Effects of Rhizobium Inoculation Mode on Growth and Development of Multiple Soybean

doi:10.6048/j.issn.1001-4330.2023.01.007

Abstract

Abstract:

【Objective】 To screen out the best inoculation method and rhizobia suitable for the growth and yield of soybeans in southern Xinjiang. 【Method】 In this experiment, 3 kinds of inoculation methods (seed dressing, seed manure, drip irrigation) and 3 kinds of rhizobia (SMH12,T6, SN7-2) were used to inoculate soybean variety Suinong 35 in the field, the design of crack zone test was adopted, the changes in soybean agronomic traits, photosynthetic characteristics, yield and yield components were measured. 【Result】 Rhizobia inoculated with different inoculation methods promoted the growth and yield of soybeans in southern Xinjiang to different extents.Seed-dressing T6 treatment had the best growth on average node number of main stem of soybean; Drip irrigation T6 treatment had the best effect on average plant height and LAI growth; Drip irrigation SN7-2 treatment promoted the growth of stem diameter, LAD, grain number per plant and pod number per plant best.Seed-dressing SN7-2 treatment had the best effect on Tr, Gs and Pn, grain weight per plant and yield of soybean. 【Conclusion】 Under the experimental conditions, the combination with the best yield effect is seed dressing-SN7-2, which reached 5,946.0 kg/hm².The combinations with better yield of drip irrigation-T6 is 5,888.6 kg/hm², seed manure-SN7-2 is 5,461.6 kg/hm², and seed manure-SMH12 is 5,446.6 kg/hm².

Key words: multiple soybean; Rhizobium; inoculation method; growth and development

摘要：

【目的】筛选出适宜新疆南疆复播大豆生长及产量最佳的接种方式及根瘤菌。【方法】采用3种接种方式(拌种、种肥、滴施)和3种根瘤菌剂(SMH12、T6、SN7-2)对大豆品种绥农35进行田间接种试验,采用裂区试验设计,测定大豆农艺性状、光合特性、产量及产量构成因素的变化。【结果】不同接种方式接种根瘤菌均能不同程度促进南疆复播大豆生长及产量。拌种T6处理对大豆平均主茎节数生长最佳;滴施T6处理对大豆平均株高、LAI生长最佳;滴施SN7-2处理对促进大豆茎粗、LAD、单株粒数、单株荚数生长最佳;拌种SN7-2处理对大豆植株Tr、Gs、Pn、单株粒重及产量为最佳。【结论】产量效果最好的组合为拌种-SN7-2,高达5 946.0 kg/hm²。产量较好的组合为滴施-T6达到5 888.6 kg/hm²,种肥-SN7-2为5 461.6 kg/hm²,种肥-SMH12为5 446.6 kg/hm²。

关键词: 复播大豆, 根瘤菌, 接种方式, 生长发育

CLC Number:

S565.1
S311

WU Shu, XU Yue, XU Yaxin, HUANG Xingjun, WU Quanzhong, CHEN Guodong, ZHAI Yunlong. Effects of Rhizobium Inoculation Mode on Growth and Development of Multiple Soybean[J]. Xinjiang Agricultural Sciences, 2023, 60(1): 52-60.

吴树, 徐玥, 胥雅馨, 黄兴军, 吴全忠, 陈国栋, 翟云龙. 根瘤菌接种方式对复播大豆生长发育的影响[J]. 新疆农业科学, 2023, 60(1): 52-60.

Figures/Tables 5

References 39

[1]	李瑞平, 李志刚, 马日亮, 等. 大豆垄上三行窄沟密植栽培群体生理研究[J]. 大豆科学, 2009, 28(1):81-84.
	LI Ruiping, LI Zhigang, MA Riliang, et al. Effect of Narrow Trenches and Compact Planting of Three Lines of Ridge on Population Physiology in Soybean[J]. Soybean Science, 2009, 28(1):81-84.
[2]	伍惠, 钟喆栋, 樊伟, 等. 8株优良大豆根瘤菌与不同地区27个大豆主栽品种的匹配性研究[J]. 大豆科学, 2017, 36(3):405-418.
	WU Hui, ZHONG Zhedong, FAN Wei, et al. Symbiotic Compatibility Among Eight Elite Soybean Rhizobia Strains and Twenty-seven Soybean Cultivars from Different Planting Regions[J]. Soybean Science, 2017, 36(3):405-418.
[3]	谢志涛, 谢甫绨, 王海英, 等. 不同种植密度和施肥水平对大豆籽粒品质的影响[J]. 种子, 2006, 25(3):60-62.
	XIE Zhitao, XIE Futi, WANG Haiying, et al. Effects of different planting density and fertilizer levelson grain quality of soybean[J]. Seeds, 2006, 25(3):60-62.
[4]	王宏光, 孙殿君, 马忠强, 等. 大豆根瘤菌HD001的分离鉴定及结瘤能力检测[J]. 大豆科学, 2014, 33(3):379-384.
	WANG Hongguang, SUN Dianjun, MA Zhongqiang, et al. Isolation and Identification of Rhizobium HD001 and Its Nodulation Capacity Test in Soybean Germplasm[J]. Soybean Science, 2014, 33(3):379-384.
[5]	龚振平, 金喜军, 马春梅, 等. 春大豆对不同来源氮素吸收利用的研究[J]. 土壤通报, 2010, 41(5):1138-1141.
	GONG Zhengping, JIN Xijun, MA Chunmei, et al. Study on the absorption and utilization of various source nitrogen by spring soybean[J]. Chinese Journal of Soil Science, 2010, 41(5):1138-1141.
[6]	姬月梅, 罗瑞萍, 赵志刚, 等. 接种根瘤菌对春大豆生长及产量的影响[J]. 种子, 2014, 33(11):75-78.
	JI Yuemei, LUO Ruiping, ZHAO Zhigang, et al. Effect of Rhizobium Inoculation on the Growth and Yield of Spring Soybean[J]. Seeds, 2014, 33(11):75-78.
[7]	马中雨, 李俊, 张永芳, 等. 大豆根瘤菌与大豆品种共生匹配性研究[J]. 大豆科学, 2008, 27(2):221-227.
	MA Zhongyu, LI Jun, ZHANG Yongfang, et al. Symbiotic Matching between Soybean Rhizobium and Soybean Cultivars[J]. Soybean Science, 2008, 27(2):221-227.
[8]	杨文钰, 雍太文, 任万军, 等. 发展套作大豆,振兴大豆产业[J]. 大豆科学, 2008, 27(1):1-7.
	YANG Wenyu, YONG Taiwen, REN Wanjun, et al. Develop Relay-planting Soybean, Revitalize Soybean Industry[J]. Soybean Science, 2008, 27(1):1-7.
[9]	谭娟. 接种俄罗斯大豆根瘤菌对大豆生长和产量的影响[J]. 作物杂志, 2007, 38(4):36-37.
	TAN Juan. Effect of inoculation of rhizobia on soybean growth and yield[J]. Crops, 2007, 38(4):36-37.
[10]	王金生, 丁宁, 吴俊江, 等. 大豆根瘤菌接种效应及竞争结瘤能力分析[J]. 大豆科学, 2017, 36(5):761-767.
	WANG Jinsheng, DING Ning, WU Junjiang, et al. Analysis of the Inoculation Effect of Soybean Rhizobiaand the Competitive Nodulation Ability[J]. Soybean Science, 2017, 36(5):761-767.
[11]	贺学礼, 赵丽莉. 陕西豆科固氮牧草的生态学研究[J]. 中国草地, 1997,(2):45-48.
	HE Xueli, ZHAO Lili. Ecological Study on the Nitrogen Fixing Forage Plants of Fabaceae Family in Shaanxi[J]. Chinese Journal of Grassland, 1997,(2):45-48.
[12]	Heichei G H, Vance C P. Nitrate-N and rhizobium strain roles in alfalfa seedling nodulation and growth[J]. Crop Science, 1979, 19(7):512-519. DOI URL
[13]	陈文新, 汪恩涛, 陈文峰. 根瘤菌-豆科植物共生多样性与地理环境的关系[J]. 中国农业科学, 2004, 37(1):81-86.
	CHEN Wenxin, WANG Entao, CHEN Wenfeng. The Relationship Between the Symbiotic Promiscuity of Rhizobiaand Legumes and Their Geographical Environments[J]. Scientia Agricultura Sinica, 2004, 37(1):81-86.
[14]	石茂玲, 邓波, 刘忠宽, 等. 5株根瘤菌接种紫花苜蓿的效果[J]. 草业科学, 2015, 32(1):101-106.
	SHI Maoling, DENG Bao, LIU Zhongkuan, et al. Inoculation effects of five rhizobial stains to alfalfa[J]. Pratacultural Science, 2015, 32(1):101-106.
[15]	Bogino P, Banchio E, Bonfiglio C, et al. Competitiveness of abradyrhizobiumsp.strain in soils containing Indigenous Rhizobia[J]. Current Microbiology, 2008, 56(1):66-72. PMID
[16]	张红侠, 冯瑞华, 关大伟, 等. 黄土高原地区优良大豆根瘤菌的筛选与接种方式研究[J]. 大豆科学, 2010, 29(6):996-1002.
	ZHANG Hongxia, FENG Ruihua, GUAN Dawei, et al. Screening of Superior Soybean Rhizobial Strains and Analyzing of Different Inoculation Methods in Loess Plateau Region of China[J]. Soybean Science, 2010, 29(6):996-1002.
[17]	李涛, 关大伟, 李俊, 等. 黄淮海地区优良大豆根瘤菌株的筛选与接种方式研究[J]. 大豆科学, 2010, 29(4):645-650.
	LI Tao, GUAN Dawei, LI Jun, et al. Screening of Superior Soybean Rhizobial Strains and Approach to Inoculation Methods for Regions of Huang Huai Hai[J]. Soybean Science, 2010, 29(4):645-650.
[18]	马霞, 王丽丽, 李卫军, 等. 不同施氮水平下接种根瘤菌对苜蓿固氮效能及种子生产的影响[J]. 草业学报, 2013, 22(1):95-102.
	MA Xia, WANG Lili, LI Weijun, et al. Effects of different nitrogen levels on nitrogen fixation and seed production of alfalfa inoculated with rhizobia[J]. Acta Prataculturae Sinica, 2013, 22(1):95-102.
[19]	梅沛沛, 王平, 李隆, 等. 新开垦土壤上构建玉米/蚕豆-根瘤菌高效固氮模式[J]. 中国生态农业学报, 2018, 26(1):62-74.
	MEI Peipei, WANG Ping, LI Long, et al. Construction of efficient nitrogen-fixing cropping pattern: Maize/faba bean intercrop with rhizobium inoculation in reclaimed low-fertility soils[J]. Chinese Journal of Eco-Agriculture, 2018, 26(1):62-74.
[20]	梁华, 陈灿灿, 陈家炜, 等. 固氮菌肥对山西道地黄芪品质及产量的影响[J]. 山西农业科学, 2019, 47(5):841-844.
	LIANG Hua, CHEN Cancan, CHEN Jiawei, et al. Effect of Nitrogen-fixing Bacteria Fertilizer on Quality and Yield of Astragalus Daodi in Shanxi Province[J]. Journal of Shanxi Agricultural Sciences, 2019, 47(5):841-844.
[21]	热比姑丽·热合曼, 郭海玉, 李卫军, 等. 地下滴灌条件下不同接种处理对苜蓿根瘤菌的定殖、结瘤和固氮的影响[J]. 草地学报, 2018, 26(2):479-487. DOI
	Rebiguli Reheman, GUO Haiyu, LI Weijun, et al. Effects of Different Inoculation Treatments on Colonization, Nodulation and Nitrogen Fixation Efficiency of Rhizobium in Alfalfa Field under Subsurface Drip Irrigation[J]. Acta Agrestia Sinica, 2018, 26(2):479-487.
[22]	胥雅馨, 徐玥, 李玲, 等. 接种根瘤菌对南疆春大豆结瘤和生长的影响[J]. 大豆科学, 2021, 40(1):98-105.
	XU Yaxin, XU Yue, LI Ling, et al. Effects of Rhizobia Inoculation on Nodulation and Growth of Spring Soybean in Southern Xinjiang[J]. Soybean Science, 2021, 40(1):98-105.
[23]	衡楠楠, 陈远学, 徐开未, 等. 大豆根瘤菌SCAUs8的接种效果、促生性及系统发育研究[J]. 微生物学通报, 2016, 43(8):1708-1714.
	HENG Nannan, CHEN Yuanxue, XU Kaiwei, et al. Field assessment of symbiotic efficiency,growth-promoting ability and phylogeny of soybean rhizobial strain SCAUs8[J]. Microbiology China, 2016, 43(8):708-1714.
[24]	Haag A F, Arnold M F, Myka K K, et al. Molecular insights into bacteroid development during rhizobium-legumesymbiosis[J]. Federation of European Microbiological Societies, 2013, 37(3):364-383.
[25]	刘莉, 周俊初, 陈华癸. 不同化合态氮浓度对大豆根瘤菌结瘤和固氮作用的影响[J]. 中国农业科学, 1998, 31(4):87-89.
	LIU Li, ZHOU Junchu, CHEN Huakui. The Effect of Different Compound Nitrogen Concentration on the Nodulation and Nitrogen Fixation in Soybean[J]. Scientia Agricultura Sinica, 1998, 31(4):87-89.
[26]	杨庆锋, 杜迎辉, 赵斌. 花生根瘤菌的应用效果研究[J]. 花生学报, 2013, 42(3):56-59.
	YANG Qingfeng, DU Yinghui, ZHAO Bin. Study on the Application Effect of Peanut Rhizobia[J]. Journal of Peanut Science, 2013, 42(3):56-59.
[27]	章孜亮, 杨庆锋, 杜迎辉, 等. 根瘤菌及不同接种方式对花生生长发育及产量的影响[J]. 花生学报, 2018, 47(3):62-65.
	ZHANG Ziliang, YANG Qingfeng, DU Yinghui, et al. Effects of Different Inoculation Methods of Rhizobia on Peanut Growth and Yield[J]. Journal of Peanut Science, 2018, 47(3):62-65.
[28]	索炎炎, 张翔, 司贤宗, 等. 氮肥管理与根瘤菌接种模式对花生生长、氮吸收利用及产量的影响[J]. 中国油料作物学报, 2018, 40(6):866-871.
	SUO Yanyan, ZHANG Xiang, SI Xianzong, et al. Effects of nitrogen fertilizer management andrhizobium inoculation methods on growth, nitrogen uptake and yield of peanut[J]. Chinese Journal of Oil Crop Sciences, 2018, 40(6):866-871.
[29]	Afza R, Hardarson G, Zapata F, et al. Effects of delayed soil and foliar N fertilization on yield and N2 fixation of soybean[J]. Plantand Soil, 1987, 97(3):361-368.
[30]	孟庆英, 张春峰, 于忠和, 等. 根瘤菌对大豆根际土壤微生物及大豆农艺性状的影响[J]. 大豆科学, 2012, 31(3):498-500.
	MENG Qingying, ZHANG Chunfeng, YU Zhonghe, et al. Effects of Rhizobia on Rhizosphere Soil Microoganisms and Agronomic Characters of Soybean[J]. Soybean Science, 2012, 31(3):498-500.
[31]	李富宽, 翟桂玉, 沈益新, 等. 施磷和接种根瘤菌对黄河三角洲紫花苜蓿生长及品质的影响[J]. 草业学报, 2005, 14(3):87-93.
	LI Fukuan, ZHAIGuiyu, SHEN Yixin, et al. Effect of superphosphate application and rhizobia inoculation on growth andforage quality of Medicago sativa in the Yellow River Delta[J]. Acta Prataculturae Sinica, 2005, 14(3):87-93.
[32]	郑浩宇, 黄炳林, 王孟雪, 等. 氮肥减施与接种根瘤菌对大豆光合与产量的影响[J]. 大豆科学, 2019, 38(3):413-420.
	ZHENG Haoyu, HUANG Binglin, WANG Mengxue, et al. The Effect of Nitrogen Fertilizer Reduction and Rhizobium Inoculation on Soybean Photosynthesis and Yield[J]. Soybean Science, 2019, 38(3):413-420.
[33]	马家斌, 于晓波, 吴海英, 等. 接种根瘤菌对西南地区大豆光合性能和固氮能力的影响[J]. 中国油料作物学报, 2020, 42(1):102-108.
	MA Jiabin, YU Xiaobo, WU Haiying, et al. Effects of inoculation of different rhizobium on photosynthetic characteristics and nitrogen fixation of soybean[J]. Chinese Journal of Oil Crop Sciences, 2020, 42(1):102-108.
[34]	胡倡, 李慧明, 伍惠, 等. 解磷菌和根瘤菌复合接种对大豆和紫云英共生固氮的影响[J]. 华中农业大学学报, 2020, 39(4):38-45.
	HU Chang, LI Huiming, WU Hui, et al. Effects of co-inoculation of phosphate-solubilizing bacteria and rhizobiumon symbiotic nitrogen fixation of soybean and Astragalus sinensis[J]. Journal of Huazhong Agricultural University, 2020, 39(4):38-45.
[35]	姬月梅, 赵志刚, 罗瑞萍, 等. 不同施氮量条件下大豆品种接种根瘤菌筛选[J]. 江苏农业科学, 2017, 45(23):80-83.
	JI Yuemei, ZHAO Zhigang, LUO Ruiping, et al. Screening of rhizobium inoculated with soybean varieties under different nitrogen application rates[J]. Jiangsu Agricultural Sciences, 2017, 45(23):80-83.
[36]	杨升辉, 王素阁, 于会勇, 等. 接种根瘤菌对夏大豆籽粒灌浆特性及品质的影响[J]. 大豆科学, 2014, 33(4):534-540.
	YANG Shenghui, WANG Suge, YU Huiyong, et al. Effects of Rhizobial Inoculation on the Grain Filling Characteristics and Quality of Summer Soybean[J]. Soybean Science, 2014, 33(4):534-540.
[37]	张俊, 郝西, 刘娟, 等. 钼肥拌种量对旱薄地花生发育及氮素积累的影响[J]. 河南农业科学, 2021, 50(3):59-66.
	ZHANG Jun, HAO Xi, LIU Juan, et al. Effect of Seed Dressing with Ammonium Molybdate on Development and Nitrogen Accumulation of Peanut in Poor Dry Land[J]. Journal of Henan Agricultural Sciences, 2021, 50(3):59-66.
[38]	赵念力, 谷维, 张俐俐, 等. 俄罗斯高效大豆根瘤菌肥对大豆主要性状及产量的影响[J]. 江苏农业科学, 2014, 42(1):72-73.
	ZHAO Nianli, GU Wei, ZHANG Lili, et al. Effects of high-efficiency soybean rhizobium fertilizer on main characters and yield of soybean in Russia[J]. Jiangsu Agricultural Sciences, 2014, 42(1):72-73.
[39]	热比姑丽·热合曼, 罗明, 李卫军. 地下滴灌苜蓿根瘤菌的接种效应[J]. 草业科学, 2020, 37(3):497-505.
	Rebiguli Reheman, LUO Ming, LI Weijun. Effect of Rhizobium inoculation on alfalfa under subsurface drip irrigation[J]. Pratacultural Science, 2020, 37(3):497-505.

接种方式 Inoculation method	根瘤菌 Nodule bacteria	株高 Plant height (cm)	茎粗 Stem diameter (cm)	主茎节数 Node number of main stem(节)
拌种 Seed dressing	SMH12	46.71^cC	0.53^bB	12.8^bcA
	T6	51.37^aA	0.51^cC	13.2^abA
	SN7-2	49.57^bB	0.56^aA	13.4^aA
	CK	42.68^dD	0.50^cC	12.6^cA
种肥 Seed manure	SMH12	48.70^aA	0.58^aA	11.4^aA
	T6	44.50^cB	0.49^bB	11.0^aA
	SN7-2	46.71^bA	0.60^aA	11.4^aA
	CK	42.90^dB	0.50^bB	11.0^aA
滴施 Drip irrigation	SMH12	47.70^cC	0.57^bB	11.6^bB
	T6	54.20^aA	0.66^aA	14.4^aA
	SN7-2	50.47^bB	0.62^aAB	13.4^aAB
	CK	42.78^dD	0.50^cC	11.8^bB
接种方式平均 Mean ofinoculation method	拌种	47.58^bB	0.52^bA	13.0^aA
	种肥	45.71^cC	0.54^abA	11.2^bB
	滴施	48.79^aA	0.59^aA	12.8^aA
根瘤菌平均 Mean ofrhizobia	SMH12	47.70^cC	0.56^abA	11.9^bA
	T6	50.02^aA	0.55^abA	12.9^aA
	SN7-2	48.91^bB	0.59^aA	12.7^aA
	CK	42.79^dD	0.50^bA	11.8^bA

接种方式 Inoculation method	根瘤菌 Nodule bacteria	株高 Plant height (cm)	茎粗 Stem diameter (cm)	主茎节数 Node number of main stem(节)
拌种 Seed dressing	SMH12	46.71^cC	0.53^bB	12.8^bcA
	T6	51.37^aA	0.51^cC	13.2^abA
	SN7-2	49.57^bB	0.56^aA	13.4^aA
	CK	42.68^dD	0.50^cC	12.6^cA
种肥 Seed manure	SMH12	48.70^aA	0.58^aA	11.4^aA
	T6	44.50^cB	0.49^bB	11.0^aA
	SN7-2	46.71^bA	0.60^aA	11.4^aA
	CK	42.90^dB	0.50^bB	11.0^aA
滴施 Drip irrigation	SMH12	47.70^cC	0.57^bB	11.6^bB
	T6	54.20^aA	0.66^aA	14.4^aA
	SN7-2	50.47^bB	0.62^aAB	13.4^aAB
	CK	42.78^dD	0.50^cC	11.8^bB
接种方式平均 Mean ofinoculation method	拌种	47.58^bB	0.52^bA	13.0^aA
	种肥	45.71^cC	0.54^abA	11.2^bB
	滴施	48.79^aA	0.59^aA	12.8^aA
根瘤菌平均 Mean ofrhizobia	SMH12	47.70^cC	0.56^abA	11.9^bA
	T6	50.02^aA	0.55^abA	12.9^aA
	SN7-2	48.91^bB	0.59^aA	12.7^aA
	CK	42.79^dD	0.50^bA	11.8^bA

接种方式 Inoculation method	根瘤菌 Rhizobia	胞间CO₂浓度Ci (μmol/mol)	蒸腾速率Tr (mmol/(m²·s))	气孔导度Gs (mol/(m²·s))	净光合速率Pn (μmol/(m²·s))
拌种 Seed dressing	SMH12	269.592^bB	8.573^bB	0.641^cB	25.255^cC
	T6	254.651^cC	9.088^aA	0.729^bA	26.433^bB
	SN7-2	239.090^dD	9.140^aA	0.772^aA	27.345^aA
	CK	291.551^aA	7.864^cC	0.479^dC	24.711^dC
种肥 Seed manure	SMH12	279.492^bB	8.289^bB	0.632^bB	27.528^bB
	T6	288.403^aA	6.121^dD	0.363^dD	22.048^dD
	SN7-2	263.468^cC	9.000^aA	0.990^aA	28.420^aA
	CK	281.332^bB	7.857^cC	0.530^cC	24.831^cC
滴施 Drip irrigation	SMH12	279.015^bA	8.301^bB	0.638^cC	25.360^bB
	T6	258.407^dC	9.042^aA	0.747^aA	27.042^aA
	SN7-2	271.026^cB	8.713^aAB	0.721^bB	25.911^bB
	CK	283.868^aA	7.268^cC	0.466^dD	24.591^cC

接种方式 Inoculation method	根瘤菌 Rhizobia	胞间CO₂浓度Ci (μmol/mol)	蒸腾速率Tr (mmol/(m²·s))	气孔导度Gs (mol/(m²·s))	净光合速率Pn (μmol/(m²·s))
拌种 Seed dressing	SMH12	269.592^bB	8.573^bB	0.641^cB	25.255^cC
	T6	254.651^cC	9.088^aA	0.729^bA	26.433^bB
	SN7-2	239.090^dD	9.140^aA	0.772^aA	27.345^aA
	CK	291.551^aA	7.864^cC	0.479^dC	24.711^dC
种肥 Seed manure	SMH12	279.492^bB	8.289^bB	0.632^bB	27.528^bB
	T6	288.403^aA	6.121^dD	0.363^dD	22.048^dD
	SN7-2	263.468^cC	9.000^aA	0.990^aA	28.420^aA
	CK	281.332^bB	7.857^cC	0.530^cC	24.831^cC
滴施 Drip irrigation	SMH12	279.015^bA	8.301^bB	0.638^cC	25.360^bB
	T6	258.407^dC	9.042^aA	0.747^aA	27.042^aA
	SN7-2	271.026^cB	8.713^aAB	0.721^bB	25.911^bB
	CK	283.868^aA	7.268^cC	0.466^dD	24.591^cC

接种方式 Inoculation method	根瘤菌 Rhizobia	单株粒重 Seed weigh per plant(g)	单株荚数 Pod number per plant	单株粒数 Seed number per plant	百粒重 100-seed weight(g)	产量 Yield (kg/hm²)
拌种 Seed dressing	SMH12	21.5±0.4^bB	38.6±0.5^cC	106.4±0.7^bB	20.2±0.3^aA	4 651.5±64.0^cC
	T6	23.7±0.3^aA	35.4±0.5^bB	98.0±1.0^aA	18.8±0.3^cB	5 208.8±22.0^bB
	SN7-2	24.3±0.4^aA	44.6±0.5^cBC	105.0±0.9^abAB	19.6±0.5^abAB	5 946.0±52.4^aA
	CK	19.6±0.9^cC	33.4±0.9^aA	88.2±0.8^aAB	19.2±0.1^bcAB	4 120.6±18.0^dD
种肥 Bacterial manure	SMH12	17.4±0.2^bA	26.2±0.8^bB	77.0±1.0^bB	20.6±0.3^aA	5 446.6±58.7^aA
	T6	13.5±0.1^dC	26.0±0.7^bB	67.0±0.7^aA	18.9±0.1^bB	3 305.3±88.4^cC
	SN7-2	17.9±0.2^aA	31.6±0.5^bB	82.6±0.9^bB	19.2±0.3^bB	5 461.6±61.7^aA
	CK	15.3±0.4^cB	25.8±0.8^aA	66.6±0.9^aA	19.1±0.1^bB	3 957.4±56.2^bB
滴施 Drip irrigation	SMH12	18.3±0.1^cC	37.4±0.9^bA	101.8±1.5^bB	16.5±0.2^cB	4 739.6±52.4^cB
	T6	22.6±0.3^aA	48..0±0.7^aA	117.0±1.2^aA	17.4±0.0^bA	5 888.6±61.0^aA
	SN7-2	19.0±0.3^bB	38..0±1.2^bA	102.6±0.9^bB	17.9±0.3^aA	4 948.4±46.7^bB
	CK	16.1±0.4^dD	36.6±0.5^bA	84.0±1.2^cC	16.4±0.3^cB	4 094.8±54.7^dC
接种方式平均 Mean of inoculation method	拌种	23.1±0.3^aA	38.0±0.4^bB	99.4±0.4^bB	19.4±0.1^aA	4 981.7±39.1^aA
	种肥	19.7±0.2^bB	27.4±0.5^cC	73.3±0.4^cC	19.5±0.1^aA	4 542.7±35.4^bB
	滴施	20.0±0.1^bB	40.0±0.4^aA	101.4±0.8^aA	17.1±0.2^bB	4 917.8±53.7^aA
根瘤菌平均 Mean of rhizobia	SMH12	19.0±0.1^cC	33.0±0.5^bB	92.3±0.6^bB	19.1±0.1^aA	4 945.9±23.5^bB
	T6	19.9±0.1^bB	37.5±0.3^aA	96.3±0.5^aA	18.4±0.1^cC	4 800.9±42.5^cB
	SN7-2	20.4±0.1^aA	38.1±0.4^aA	97.2±0.5^aA	18.9±0.3^bB	5 452.0±12.5^aA
	CK	17.0±0.4^dD	31.9±0.6^cB	79.6±0.7^cC	18.2±0.1^dC	4 057.6±5.5^dC