Effects of Hydrogen-Rich Water on Barley Seed Germination under Drought Stress

doi:10.6048/j.issn.1001-4330.2022.01.010

Abstract

Abstract:

【Objective】 To study the effects of hydrogen-rich water (HRW) on barley seeds germination under drought stress and clarify the role of HRW in drought response. 【Method】 The changes of germination characteristics, osmotic regulation substance content, antioxidant enzyme activity and malondialdehyde content of barley seeds (Xinpi 6) under the concentration of semi-lethal polyethylene glycol-6000 were measured by soaking seeds with different concentrations (0,25%, 50%,75%,100%).【Result】 25% and 50% HRW soaking seeds could significantly improve the germination quality of barley seeds under drought stress. Under the above concentration of HRW treatment, the germination potential, germination rate and germination index increased significantly (P<0.05); the contents of soluble protein, soluble sugar and free proline increased to different degrees; the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) also increased to different levels and the expression of malondialdehyde (MDA) decreased significantly (P<0.05). 【Conclusion】 The appropriate concentration of HRW can enhance the tolerance of barley seeds to drought stress by enhancing the ability of osmotic regulation and antioxidation.

Key words: hydrogen-rich water; drought stress; barley(Hordeum Vulgare L.); germination

摘要：

【目的】研究富氢水对干旱胁迫下大麦种子萌发的影响,分析富氢水在干旱应答中的作用。【方法】以大麦品种新啤6号为试材,采用不同浓度(0、25%、50%、75%、100%)富氢水浸种处理,测定半致死聚乙二醇-6000浓度下大麦种子发芽特性、渗透调节物质含量、抗氧化酶活性及丙二醛含量变化。【结果】25%及50%富氢水浸种能明显改善干旱胁迫下大麦种子萌发质量。富氢水处理下,种子发芽率、发芽势、发芽指数显著增加;可溶性糖、可溶性蛋白及游离脯氨酸含量均有不同程度提升;丙二醛积累量显著下降;超氧化物歧化酶、过氧化物酶、过氧化氢酶活性明显增强。【结论】适宜浓度的富氢水能够通过提升渗透调节能力和抗氧化能力的途径增强大麦种子对干旱胁迫的耐受性。

关键词: 富氢水, 干旱胁迫, 大麦(Hordeum Vulgare L.), 萌发

CLC Number:

S512.3

SONG Ruijiao, FENG Caijun, QI Juncang. Effects of Hydrogen-Rich Water on Barley Seed Germination under Drought Stress[J]. Xinjiang Agricultural Sciences, 2022, 59(1): 79-85.

宋瑞娇, 冯彩军, 齐军仓. 富氢水对干旱胁迫下大麦种子萌发的影响[J]. 新疆农业科学, 2022, 59(1): 79-85.

Figures/Tables 4

References 26

[1]	刘慧, 薛凤蕊. 中国大麦贸易现状及发展趋势[J]. 农业展望, 2015, 11(8):66-69.
	LIU Hui, XUE Fengrui. Trade status and development trend of China’s barley[J]. Agricultural Outlook, 2015, 11(8):66-69.
[2]	Mahajan S, Tuteja N. Cold, salinity and drought stresses: An overview[J]. Archives of Biochemistry and Biophysics, 2005, 444(2):139-158. PMID
[3]	Renwick G M, Sieĝel S M, Giumarro C. Hydrogen metabolism in higher plants[J]. Plant Physiology, 1964, 39:303-306. DOI PMID
[4]	Xu S, Zhu S S, Jiang Y L, et al. Hydrogen-rich water alleviates salt stress in rice during seed germination[J]. Plant and Soil, 2013, 370:47-57. DOI URL
[5]	Wang Y, Duan X L, Xu S, et al. Linking hydrogen-mediated boron toxicity tolerance with improvement of root elongation, water status and reactive oxygen species balance: A case study for rice[J]. Annals of Botany, 2016, 118:1279-1291. PMID
[6]	Xu D K, Cao H, Fang W, et al. Linking hydrogen-enhanced rice aluminum tolerance with the reestablishment of GA/ABA balance and miRNA-modulated gene expression: A case study on germination[J]. Ecotoxicology and Environmental Safety, 2017, 145:303-312. DOI URL
[7]	田婧芸, 张慧洁, 陕嘉楠, 等. 富氢水处理对铜胁迫下小麦幼苗生长及其细胞结构的影响[J]. 河南农业大学学报, 2018, 52(2):193-198.
	TIAN Jingyun, ZHANG Huijie, SHAN Jianan, et al. Effects of hydrogen-enriched water on growth and cell structure of wheat seedlings under[J]. Journal of Henan Agricultural University, 2018, 52(2):193-198.
[8]	李合生, 孙群, 赵世杰. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
	LI Hesheng, SUN Qun, ZHAO Shijie. Principles and techniques of plant physiological and biochemical experiments [M]. Beijing: Higher Education Press, 2000.
[9]	Bradford M M. A rapid and sensitive method for the of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976
[10]	高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006: 210-211.
	GAO Junfeng. Experimental guidance of plant physiology [M]. Beijing: Higher Education Press, 2006: 210-211.
[11]	Maehly A C, Chance B. The assay of catalases and peroxidases[J]. Methods of Biochemical Analysis, 1954, 1:357-424.
[12]	代小冬, 杨育峰, 朱灿灿, 等. 谷子萌芽期对干旱胁迫的响应及抗旱性评价[J]. 华北农学报, 2015, 30(4):139-144.
	Dai X D, Yang Y F, Zhu C C, et al. Seed germination response to drought resistance evaluation of foxtail millet[J]. Acta Agriculturae Boreali-Sinica, 2015, 30(4):139-144.
[13]	张瑜, 严琳玲, 虞道耿, 等. PEG胁迫下85份引进柱花草种子的萌发特性[J]. 热带作物学报, 2020, 41(4):676-684.
	ZHANG Yu, YAN Linling, YU Daogeng, et al. Germination characteristics of 85 introduced stylosanthesseed under PEG stress[J]. Chinese Journal of Tropical Crops, 2020, 41(4):676-684.
[14]	Heydecker W, Higgins J, Gulliver RL. Accelerated germination by osmotic seed treatment[J]. Nature, 1973, 246:42-44 DOI URL
[15]	贾祥, 陈盼盼, 王艳琳, 等. 三种西藏野生草木樨种子萌发期对PEG胁迫的响应及耐旱性评价[J]. 分子植物育种, 1-17.
	JIA Xiang, CHEN Panpan. WANG Yanlin, et al. Response of three wild melilotus in tibet to PEG stress in seed germination period and drought tolerant evaluation[J]. Molecular Plant Breeding, 1-17.
[16]	程波, 胡生荣, 高永, 等. PEG模拟干旱胁迫下5种紫花苜蓿萌发期抗旱性的评估[J]. 西北农林科技大学学报(自然科学版), 2019, 47(1):53-59.
	CHENG Bo, HU Shengrong, GAO Yong, et al. Drought resistance of 5 alfalfa species at germination period under PEG simulated drought stress[J]. Journal of Northwest A & F University(Natural Science ), 47(1):53-59.
[17]	王海宁, 张建利, 冯林, 等. 温度和干旱胁迫对3种牧草种子萌发的影响[J]. 草业科学, 2009, 26(8):87-92.
	WANG Haining, ZHANG Jianli, FENG Lin, et al. Effect of temperature and drought stress on seed germination of three forage species[J]. Pratacultural Science, 2009, 26(8):87-92.
[18]	Zhao X Q, Chen Q H, Wang Y M, et al. Hydrogen-rich water induces aluminum tolerance in maize seedlings by enhancing antioxidant capacities and nutrient homeostasis[J]. Ecotoxicology and Environmental Safety, 2017, 144:369-379. DOI URL
[19]	Chen Q H, Zhao X Q, Lei D K, et al. Hydrogen-rich water pretreatment alters photosynthetic gas exchange, chlorophyll fluorescence, and antioxidant activities in heat-stressed cucumber leaves[J]. Plant Growth Regulation, 2017, 83:69-82. DOI URL
[20]	路之娟, 张永清, 张楚. 干旱胁迫对不同苦荞品种苗期生长和根系生理特征的影响[J]. 西北植物学报, 2018, 38(1):112-120.
	LU Zhijuan, ZHANG Yongqing, ZHANG Chu. The seedling growth and root physiological traits of Fagopyrum tataricumcultivars under drought stress[J]. Acta Botanica Boreali-OccidentaliaSinica, 2018, 38(1):112-120.
[21]	刘丰娇, 蔡冰冰, 孙胜楠, 等. 富氢水浸种增强黄瓜幼苗耐冷性的作用及其生理机制[J]. 中国农业科学, 2017, 50(5):881-889.
	LIU Fengjiao, CAI Bingbing, SUN Shengnan, et al. Effect of hydrogen-rich water soaked cucumber seeds on cold tolerance and its physiological mechanism in cucumber seedlings. Scientia Agricultura Sinica, 2017, 50(5):881-889.
[22]	Su J C, Zhang Y H, Nie Y, et al. Hydrogen-induced osmotic tolerance is associated with nitric oxide-mediated proline accumulation and reestablishment of redox balance in alfalfa seedlings[J]. Environmental and Experimental Botany, 2018, 147:249-260. DOI URL
[23]	Sun Q Y, Li Z Y, Li H Y, et al. Effects of water stress on germination and physiological characteristics of nine Elymus sibiricus L. accessons[J]. Chinese Journal of Grassland, 2016, 38(3):19-25,95.
[24]	Gill S S, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J]. Plant Physiology and Biochemistry, 2010, 48:909-930. DOI URL
[25]	Apel K, Hirt H. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction[J]. Annual Review of Plant Biology, 2004, 55:373-399. DOI URL
[26]	Ohsawa I, Ishikawa M, Takahashi K, et al. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals[J]. Nature Medicine, 2007, 13:688-694. DOI URL

富氢水浓度 HRW level (%)	发芽率 Germination rate(%)	发芽势 Germination potential(%)	发芽指数 Germination index
0(CK)	60.0±2.0^c	34.0±1.2^b	52.51±0.16^c
25	73.3±0.7^a	49.3±4.1^a	75.72±4.08^a
50	69.3±0.7^b	47.3±4.4^a	70.48±3.81^a
75	67.3±0.7^b	34.0±1.2^b	60.23±0.26^b
100	62.7±1.3^c	28.0±2.3^b	51.92±0.69^c

富氢水浓度 HRW level (%)	发芽率 Germination rate(%)	发芽势 Germination potential(%)	发芽指数 Germination index
0(CK)	60.0±2.0^c	34.0±1.2^b	52.51±0.16^c
25	73.3±0.7^a	49.3±4.1^a	75.72±4.08^a
50	69.3±0.7^b	47.3±4.4^a	70.48±3.81^a
75	67.3±0.7^b	34.0±1.2^b	60.23±0.26^b
100	62.7±1.3^c	28.0±2.3^b	51.92±0.69^c

富氢水浓度 HRW level (%)	可溶性糖含量 Soluble sugar content (mg/g FW)	可溶性蛋白含量 Soluble protein content (mg/g FW)	游离脯氨酸含量 Free proline content (μg/g FW)
0(CK)	206.12±1.069^c	39.03±1.78^c	486.90±44.59^b
25	280.67±2.972^a	45.51±1.12^ab	565.19±19.69^ab
50	271.04±1.281^a	46.85±2.03^a	645.39±23.72^a
75	224.46±5.351^b	40.85±2.45^bc	632.69±21.24^a
100	226.64±8.753^b	40.34±0.84^bc	511.51±39.86^b

富氢水浓度 HRW level (%)	可溶性糖含量 Soluble sugar content (mg/g FW)	可溶性蛋白含量 Soluble protein content (mg/g FW)	游离脯氨酸含量 Free proline content (μg/g FW)
0(CK)	206.12±1.069^c	39.03±1.78^c	486.90±44.59^b
25	280.67±2.972^a	45.51±1.12^ab	565.19±19.69^ab
50	271.04±1.281^a	46.85±2.03^a	645.39±23.72^a
75	224.46±5.351^b	40.85±2.45^bc	632.69±21.24^a
100	226.64±8.753^b	40.34±0.84^bc	511.51±39.86^b

富氢水浓度 HRW level (%)	丙二醛含量 MDA content [μmol/(g FW)]	超氧化物歧化酶活性 SOD activity [U/(gFW·min)]	过氧化物酶活性 POD activity [U/(gFW·min)]	过氧化氢酶活性 CAT activity [U/(gFW·min)]
0(CK)	4.77±0.04^a	229.33±10.00^b	36.36±1.69^c	212.95±12.63^d
25	4.08±0.07^b	343.23±18.28^a	46.45±2.66^ab	337.26±52.20^bc
50	4.37±0.21^b	290.17±3.04^ab	50.76±4.43^a	467.44±8.20^a
75	4.36±0.06^b	318.97±13.98^a	41.72±0.69^bc	404.35±44.21^ab
100	4.46±0.09^a	259.20±11.86^ab	40.58±0.91^bc	285.14±17.13^cd