Abstract:

【Objective】 To discover candidate genes related to salt tolerance in potatoes and provide theoretical basis for the identification and evaluation of salt tolerance in potatoes and the exploration of salt tolerance genes. 【Methods】 The salt tolerant variety Jinshu 16 and salt sensitive variety Jizhangshu 12 were used as test materials. Salt stress treatments were carried out using 0%, 0.5%, 1%, and 1.5% NaCl solutions. On the 8th day, plant phenotypes and physiological and biochemical indicators were analyzed. Subsequently, a 1% salt concentration was selected for treatment, and transcriptome sequencing and bioinformatics analysis were performed on the two varieties. 【Results】 Compared to Jizhangshu 12, Jinshu 16 had lower malondialdehyde content under salt stress conditions. Transcriptome data showed significant differences in plant hormone signaling pathways and flavonoid metabolism pathways between the two varieties. 【Conclusion】 Based on the above results, several differentially expressed genes related to plant hormone signal transduction and flavonoid metabolism are screened, which provides genetic resources for improving salt tolerance in potatoes.

Key words: potato; salt stress; transcriptome sequencing; plant hormone signaling pathways; flavonoids metabolic pathways

摘要：

【目的】研究与马铃薯耐盐性相关的候选基因,为马铃薯耐盐性鉴定与评价和耐盐基因的挖掘提供理论依据。【方法】以耐盐品种晋薯16号及盐敏感品种冀张薯12号为试材,采用0%、0.5%、1%和1.5% NaCl溶液进行盐胁迫处理,在第8 d时,分析植株表型和生理生化指标,随后选择1%的盐浓度处理,分析2个品种进行转录组测序和生物信息学。【结果】相较于冀张薯12号,晋薯16号在盐胁迫条件下,具有较低的丙二醛含量。植物激素信号转导途径和类黄酮代谢途径在2个马铃薯品种中显示出明显的差异。【结论】筛选出与植物激素信号转导和类黄酮代谢相关的差异表达基因。

关键词: 马铃薯, 盐胁迫, 转录组测序, 植物激素信号途径, 类黄酮代谢途径