Effects of Compound Salt Stress on Germination of 7 Maize Varieties

doi:10.6048/j.issn.1001-4330.2022.08.005

Abstract

Abstract:

【Objective】 To screen out salt-tolerant maize varieties suitable for planting in salinized soil in Xinjiang. 【Methods】 Seven maize materials were prepared with salt solution in natural saline soil in the field. The germination potential, germination rate, length of radicle and germ, fresh weight of radicle and germ could bewere used to reflect the salt tolerance of seeds at germination stage from multiple angles. 【Results】 The results showed that under salt stress, the germination rate, germination potential, number of r oots, length and weight of embryo and radicle of all maize varieties decreased gradually with the increase of salt concentration, and there were great differences among different varieties. Among them, the related indexes of New Xinyu 102 and Xinyu 110 decreased less than that of the control under the same salt concentration, showing a strong tolerance to salt, while the indexes of KWS3376 and KWS2564 decreased more than that of the control, showing a sensitivitysensitivity to salt, showing and a salt intolerance. 【Conclusion】 Xinyu 102 and Xinyu 110 were selected as maize varieties with high salt tolerance. Under these conditions, the germination potential, germination rate, length and weight of germ and radicle of maize decreased significantly when the content was over 0.9%, which seriously affected the germination of maize.

Key words: salinization; corn; germination; salt stress

摘要：

【目的】筛选出适合新疆盐渍化土壤种植的耐盐玉米品种。【方法】利用田间自然盐渍土配置盐溶液,对7份玉米材料进行萌发期耐盐试验,测定发芽势、发芽率、胚根胚芽长、胚根胚芽鲜重等指标种子萌发期耐盐性。【结果】在盐胁迫下,7个玉米品种的发芽率、发芽势、发根数、胚芽和胚根的长及重量等指标随盐分浓度的增加逐渐下降,在不同品种之间下降幅度有较大差异,其中新玉102号和新玉110号品种的相关指标在同等盐分浓度下各项指标与对照相比下降幅度较少,对盐分的耐受能力较强,而KWS3376和KWS2564品种的指标与对照比降幅较大,对盐分较为敏感,表现为不耐盐。【结论】筛选出较耐盐的玉米品种是新玉102号和新玉110号品种,在此条件下含量超过0.9%时,玉米的发芽势、发芽率、胚芽和胚根长度及重量等指标都有明显的降低,影响玉米萌发。

关键词: 盐渍化, 玉米, 萌发, 盐胁迫

CLC Number:

S513

QI Tong, HUANG Jian, XU Fei, HAN Dengxu. Effects of Compound Salt Stress on Germination of 7 Maize Varieties[J]. Xinjiang Agricultural Sciences, 2022, 59(8): 1855-1863.

祁通, 黄建, 徐菲, 韩登旭. 复合盐胁迫对7个玉米品种萌发的影响[J]. 新疆农业科学, 2022, 59(8): 1855-1863.

Figures/Tables 8

References 15

[1]	Shabala S. Learning from halophytes: physiological basis and strategies to improve abiotic stress tolerance in crops[J]. Annals of Botany, 2013, 112( 7):1209-1221. DOI PMID
[2]	田长彦, 买文选, 赵振勇. 新疆干旱区盐碱地生态治理关键技术研究[J]. 生态学报, 2016, 36(22):7064-7068.
	TIAN Changyan, MAI Wenxuan, ZHAO Zhenyong. Study on key technologies of ecological management of saline alkali land in arid area of Xinjiang[J]. ActaEcologica Sinica, 2016, 36(22):7064-7068.
[3]	姜奇彦, 胡正, 张辉, 等. 大豆种质资源耐盐性鉴定与研究[J] .植物遗传资源学报, 2012, 13(5):726-732.
	Jiang Qiyan, Hu Zheng, Zhang Hui, et al. Evaluation for salt tolerance in soy-bean cultivars[J]. Journal of Plant Genetic Resources, 2012, 13(5):726-732.
[4]	袁海, 何鹏飞, 武君洁, 等. 盐胁迫对耐盐和盐敏感玉米幼苗生长和生理特性的影响[J]. 江苏农业科学, 2019, 47(19):86-89
	YUAN Hai, HE Pengfei, WU Junjie, et al. Effects of salt stress on growth and biological traits of salt-tolerant and salt-sensitive maize seedlings[J]. Jiangsu Agricultural Sciences, 2019, 47(19):86-89
[5]	李文阳, 胡秀娟, 王长进, 等. 盐胁迫对不同品种玉米苗期生长与叶片光合特性的影响[J]. 生态科学, 2019, 38(2): 51-55.
	LI Wenyang, HU Xiujuan, WANG Changjin, et al. Effect of salt stress on photosynthetic characteristics of leaves in the seedling stage of different maize cultivars[J]. Ecological Science, 2019, 38(2): 51-55.
[6]	邓杰, 孙丽芳, 王霞, 等. 89份玉米自交系萌发期耐盐碱性综合评价[J]. 玉米科学, 2020, 28(4):15-21.
	DENG Jie, SUN Lifang, WANG Xia, et al. Comprehensive Evaluation of Salt Tolerance and Alkalinity of 89 Maize Inbred Lines during Germination[J]. Journal of Maize Sciences, 2020, 28(4):15-21.
[7]	刘燕, 杨伟, 马晖玲, 等. 盐胁迫对6种草地早熟禾幼苗生理特性的影响[J]. 甘肃农业大学学报, 2019, 54(5):140-150,162.
	LIU Yan, YANG Wei, MA Huiling, et al. Effects of salt stress on seedling physiological characteristics of six kentucky bluegrass[J]. Journal of Gansu Agricultural University, 2019, 54(5):140-150,162.
[8]	王志恒, 魏玉清, 邹芳, 等. PEG-6000和盐碱胁迫对甜高粱种子萌发影响研究[J]. 种子, 2019, 38(5):39-43.
	WANG Zhiheng, WEI Yuqing, ZOU Fang, et al. Study on Effects of PEG-6000 and Saline-alkali Stress on Seed Germination of Sweet Sorghum[J]. Seed, 2019, 38(5):39-43.
[9]	李源, 刘贵波, 高洪文, 等. 紫花苜蓿种质耐盐性综合评价及盐胁迫下的生理反应[J]. 草业学报. 2010, 19(4):79-86.
	LI Yuan, LIU Guibo, GAO Hongwen, et al. A comprehensive evaluation of salt-tolerance and the physiological response of medicago sativa at the seedling stage[J]. Acta Prataculturae Sinica, 2010, 19(4): 79-86.
[10]	张毅, 侯维海, 冯西博, 等. 有色大麦种质芽期耐盐性鉴定[J]. 植物遗传资源学报, 2019,(20):1-10.
	ZHANG Yi, HOU Weihai, FENG Xibo, et al. Evaluation of salt tolerance of colored barley germplasm during germination[J]. Journal of Plant Ge-netic Resources, 2019,(20): 1-10.
[11]	高英波, 张慧, 薛艳芳, 等. 不同夏玉米品种耐盐性综合评价与耐盐品种筛选[J]. 玉米科学, 2020, 28(2):33-40.
	GAO Yingbo, ZHANG Hui, XUE Yanfango, et al. Comprehensive Evaluation of Salt Tolerance and Screening for Salt Tolerance Accessions of Different Summer Maize Varieties[J]. Journalof Maize Sciences. 2020, 28(2):33-40.
[12]	景宇鹏, 李跃进, 蔺亚莉, 等. 玉米耐Na₂CO₃胁迫的生理响应及耐盐碱性综合评价[J]. 中国土壤与肥料, 2019,(5):179-186.
	JING Yupeng, LI Yuejin, LIN Yali, et al. Comprehensive evaluation of physiological response and saline-alkali resistance of maize to Na₂CO₃ stress[J]. Soil and Fertilizer Sciences in China, 2019,(5):179-186.
[13]	刘丽丽, 乔梓启, 杜明, 等. 一种基于综合指标的耐盐方程式准确预测玉米耐盐性[J]. 植物生理学报, 2022, 58(2):458-468.
	LIU Lili, QIAO Ziqi, DU Ming, et al. A salt tolerance equation based on comprehensive indexes accurately predicts salt tolerance of maize inbred lines[J]. Plant Physiology Journal, 2022, 58(2):458-468.
[14]	杨书华, 张春宵, 朴明鑫, 等. 69份玉米自交系的苗期耐盐碱性分析[J]. 种子, 2011, 30(3):1-6.
	YANG Shuhua, ZHANG Chunxiao, PIAO Mingxin, et al. Analysis on salt and alkaline tolerance of sixty-nine maize inbred lines at seedling stage[J]. Seed, 2011, 30(3): 1-6.
[15]	张海艳, 赵海军. 不同品种玉米萌发期和苗期耐盐性综合评价[J]. 玉米科学, 2016, 24(5):61-67.
	ZHANG Haiyan, ZHAO Haijun. Comprehensive evaluation of salt toleranceof different corn varieties at the germination and seedling stages[J]. Journal of Maize Sciences, 2016, 24(5): 61-67.

品种 Variety	盐分浓度Salt concentration
品种 Variety	0	0.6%	0.9%	1.2%	1.5%
KWS2564	3±1.3^b	2±0.7^b	1.3±0.3^c	1.1±0^c	1.2±0.3^c
新玉110号 Xinyu 110	5.1±0.7^a	3.3±0.1^b	3.0±0.4^ab	3.0±0.4^a	2.3±0^a
新玉54号 Xinyu 54	4.5±1.0^ab	3.5±0.4^ab	2.4±0.4^bc	2.1±0.8^abc	2.0±0.2^ab
新玉102号 Xinyu 102	4.1±0.5^ab	4.6±0.4^a	3.6±0.2^a	2.6±0.5^a	2.3±0.1^a
新玉29号 Xinyu 29	4.1±0.5^ab	3.3±0.2^b	2.3±0.6^bc	2.3±0.3^ab	1.5±0.4^abc
KWS9384	4.4±0.2^ab	2.7±0.4^bc	2.4±0.3^bc	2.3±0.4^ab	1.5±0.3^bc
KWS3376	2.8±0.1^b	2.0±0.3^b	2.0±0.6^bc	1.6±0.2^bc	1.1±0.5^c
均值 Mean value	4.0±1.0	3.1±0.9	2.4±0.8	2.3±0.7	1.7±0.5
F值	3.554*	15.619**	9.273**	9.542**	9.613**

品种 Variety	盐分浓度Salt concentration
品种 Variety	0	0.6%	0.9%	1.2%	1.5%
KWS2564	3±1.3^b	2±0.7^b	1.3±0.3^c	1.1±0^c	1.2±0.3^c
新玉110号 Xinyu 110	5.1±0.7^a	3.3±0.1^b	3.0±0.4^ab	3.0±0.4^a	2.3±0^a
新玉54号 Xinyu 54	4.5±1.0^ab	3.5±0.4^ab	2.4±0.4^bc	2.1±0.8^abc	2.0±0.2^ab
新玉102号 Xinyu 102	4.1±0.5^ab	4.6±0.4^a	3.6±0.2^a	2.6±0.5^a	2.3±0.1^a
新玉29号 Xinyu 29	4.1±0.5^ab	3.3±0.2^b	2.3±0.6^bc	2.3±0.3^ab	1.5±0.4^abc
KWS9384	4.4±0.2^ab	2.7±0.4^bc	2.4±0.3^bc	2.3±0.4^ab	1.5±0.3^bc
KWS3376	2.8±0.1^b	2.0±0.3^b	2.0±0.6^bc	1.6±0.2^bc	1.1±0.5^c
均值 Mean value	4.0±1.0	3.1±0.9	2.4±0.8	2.3±0.7	1.7±0.5
F值	3.554*	15.619**	9.273**	9.542**	9.613**

品种 Variety	盐分浓度Salt concentration
品种 Variety	0	0.6%	0.9%	1.2%	1.5%
KWS2564	2.6±0.4^a	2.0±0.2^a	1.8±0.5^bc	1.2±0.1^b	0.7±0.3^c
新玉110号 Xinyu 110	2.5±0.3^a	1.6±0.8^a	2.3±0.4^b	2.1±0.2^a	1.4±0.3^bc
新玉54号 Xinyu 54	2.6±0.3^a	1.9±0.4^a	1.9±0.3^bc	1.7±0.2^bc	1.1±0.3^bc
新玉102号 Xinyu 102	2.7±0.2^a	2.6±0.4^a	2.5±0.2^a	2.2±0.3^a	2.0±0.5^a
新玉29号 Xinyu 29	2.3±0.8^a	1.4±0.4^a	1.6±0.5^bc	1.3±0.2^bc	1.1±0.2^bc
KWS9384	2.8±0.2^a	2.5±1.2^a	1.8±0.7^bc	1.6±0.4^bc	1.7±0.1^b
KWS3376	2.3±0.4^a	1.7±0.4^a	1.5±0.3^bc	1.8±0.2^bc	1.5±0.2^b
均值 Mean value	2.6±0.4	1.9±0.7	1.9±0.6	1.7±0.4	1.4±0.6
F值	0.637ns	1.669ns	2.830*	3.433*	10.837**

品种 Variety	盐分浓度Salt concentration
品种 Variety	0	0.6%	0.9%	1.2%	1.5%
KWS2564	2.6±0.4^a	2.0±0.2^a	1.8±0.5^bc	1.2±0.1^b	0.7±0.3^c
新玉110号 Xinyu 110	2.5±0.3^a	1.6±0.8^a	2.3±0.4^b	2.1±0.2^a	1.4±0.3^bc
新玉54号 Xinyu 54	2.6±0.3^a	1.9±0.4^a	1.9±0.3^bc	1.7±0.2^bc	1.1±0.3^bc
新玉102号 Xinyu 102	2.7±0.2^a	2.6±0.4^a	2.5±0.2^a	2.2±0.3^a	2.0±0.5^a
新玉29号 Xinyu 29	2.3±0.8^a	1.4±0.4^a	1.6±0.5^bc	1.3±0.2^bc	1.1±0.2^bc
KWS9384	2.8±0.2^a	2.5±1.2^a	1.8±0.7^bc	1.6±0.4^bc	1.7±0.1^b
KWS3376	2.3±0.4^a	1.7±0.4^a	1.5±0.3^bc	1.8±0.2^bc	1.5±0.2^b
均值 Mean value	2.6±0.4	1.9±0.7	1.9±0.6	1.7±0.4	1.4±0.6
F值	0.637ns	1.669ns	2.830*	3.433*	10.837**

品种 Variety	盐分浓度Salt concentration
品种 Variety	0	0.6%	0.9%	1.2%	1.5%
KWS2564	2.59±0.11^a	1.08±0.08^cd	0.8±0.03^b	0.46±0.01^c	0.55±0.03^c
新玉110号 Xinyu 110	3.48±0.01^a	2.57±0.05^ab	2.12±0.17^ab	1.78±0.1^ab	1.47±0.03^ab
新玉54号 Xinyu 54	2.58±0.12^a	2.74±0.03^ab	1.58±0.18^b	1.83±0.07^ab	0.88±0.07^bc
新玉102号 Xinyu 102	3.37±0.22^a	3.2±0.12^a	3.01±0.02^a	2.16±0.12^a	1.83±0.04^a
新玉29号 Xinyu 29	2.98±0.1^a	1.52±0.08^bcd	0.93±0.05^b	1.18±0.02^abc	0.61±0.02^c
KWS9384	3.13±0.06^a	2.26±0.14^abc	1.99±0.02^ab	1.27±0.06^abc	0.74±0.04^bc
KWS3376	2.27±0.02^a	0.81±0.05^d	0.8±0.06^b	0.76±0.04^bc	0.49±0.08^c
均值 Mean value	2.91±0.12	2.03±0.16	1.69±0.17	1.35±0.11	0.94±0.09
F值	1.292ns	9.301**	7.387**	6.155*	9.019**